• Title/Summary/Keyword: Well resistance

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Suppression of UDP-glycosyltransferase-coding Arabidopsis thaliana UGT74E2 Gene Expression Leads to Increased Resistance to Psuedomonas syringae pv. tomato DC3000 Infection

  • Park, Hyo-Jun;Kwon, Chang-Seob;Woo, Joo-Yong;Lee, Gil-Je;Kim, Young-Jin;Paek, Kyung-Hee
    • The Plant Pathology Journal
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    • v.27 no.2
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    • pp.170-182
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    • 2011
  • Plants possess multiple resistance mechanisms that protect themselves against pathogen attack. To identify unknown components of the defense machinery in Arabidopsis, gene-expression changes were monitored in Arabidopsis thaliana under 18 different biotic or abiotic conditions using a DNA microarray representing approximately 25% of all Arabidopsis thaliana genes (www.genevestigator.com). Seventeen genes which are early responsive to salicylic acid (SA) treatment as well as pathogen infection were selected and their T-DNA insertion mutants were obtained from SALK institute. To elucidate the role of each gene in defense response, bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000 was inoculated onto individual T-DNA insertion mutants. Four mutants exhibited decreased resistance and five mutants displayed significantly enhanced resistance against Pst DC3000-infection as measured by change in symptom development as compared to wild-type plants. Among them, member of uridin diphosphate (UDP)-glycosyltransferase (UGT) was of particular interest, since a UGT mutant (At1g05680) showed enhanced resistance to Pst-infection in Arabidopsis. In systemic acquired resistance (SAR) assay, this mutant showed enhanced activation of SAR. Also, the enhanced SAR correlated with increased expression of defense-related gene, AtPR1. These results emphasize that the glycosylation of UGT74E2 is a part of the SA-mediated disease-resistance mechanism.

Identification of Novel Source of Resistance and Differential Response of Allium Genotypes to Purple Blotch Pathogen, Alternaria porri (Ellis) Ciferri

  • Nanda, Satyabrata;Chand, Subodh Kumar;Mandal, Purander;Tripathy, Pradyumna;Joshi, Raj Kumar
    • The Plant Pathology Journal
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    • v.32 no.6
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    • pp.519-527
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    • 2016
  • Purple blotch, caused by Alternaria porri (Ellis) Cifferi, is a serious disease incurring heavy yield losses in the bulb and seed crop of onion and garlic worldwide. There is an immediate need for identification of effective resistance sources for use in host resistance breeding. A total of 43 Allium genotypes were screened for purple blotch resistance under field conditions. Allium cepa accession 'CBT-Ac77' and cultivar 'Arka Kalyan' were observed to be highly resistant. In vitro inoculation of a selected set of genotypes with A. porri, revealed that 7 days after inoculation was suitable to observe the disease severity. In vitro screening of 43 genotypes for resistance to A. porri revealed two resistant lines. An additional 14 genotypes showed consistent moderate resistance in the field as well as in vitro evaluations. Among the related Allium species, A. schoenoprasum and A. roylei showed the least disease index and can be used for interspecific hybridization with cultivated onion. Differential reaction analysis of three A. porri isolates (Apo-Chiplima, Apn-Nasik, Apg-Guntur) in 43 genotypes revealed significant variation among the evaluated Allium species (P = 0.001). All together, the present study suggest that, the newly identified resistance sources can be used as potential donors for ongoing purple blotch resistance breeding program in India.

Characteristics of Membrane Fouling in the Membrane-Coupled Activated Sludge (MCAS) System (막격합형 활성슬러지 시스템에서 막오염 특성의 분석)

  • 김재석;이정학
    • Membrane Journal
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    • v.8 no.3
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    • pp.130-137
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    • 1998
  • Membrane fouling characteristics in the membrane-coupled activated sludge system were investigated. The influence of the floc size variation on the filtration resistance was analyzed using resistance-in-series model and mixed liquor was fractionated into three components to verify which component would give rise to a major contribution to the total resistance. The microbial floc size was rapidly reduced during the initial 4~6 hours of operation, and then decreased slightly but steadily, followed by leveling off at the size of 20 $\mu$m. The specific resistance of activated sludge increased with operation time, and measured values of specific resistance were matched well with the values estimated on the basis of the mean particle size in the mixed liquor. The contribution of soluble organics and cells to the total resistance was relativdy small compared with that of the supematant. Colloidal particles in the supematant showed much higher specific resistance than that of microbial floc, and played the most important role in the cake resistance.

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System-level Analysis of a Fan-motor Assembly for Vacuum Cleaner (진공청소기용 팬-모터 어셈블리의 시스템-레벨 분석)

  • Park, Chang-Hwan;Park, Kyung-Hyun;Chang, Kyung-Sik
    • The KSFM Journal of Fluid Machinery
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    • v.20 no.1
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    • pp.5-14
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    • 2017
  • A fan-motor assembly in a vacuum cleaner is analyzed through system-level analysis method. This system consisted of three components, a fan, motor, and the flow resistance of the motor, or of the vacuum cleaner. System-level analysis method is characterized by the combination of torque matching at a constant throttling condition between the fan and the motor and the pressure drop at a constant flow rate due to the flow resistance of the motor, or of the vacuum cleaner. The performance characteristics of the fan-motor assembly and the vacuum cleaner system could be predicted over the whole range of operation, based on the characteristics of each component. The predicted performance of the vacuum cleaner system through system-level analysis agreed well with the experimental results within 4.5% difference of pressure and 6% difference of the efficiency. The effect of flow resistance of a motor is investigated and it is found that the efficiency decrease of fan-motor assembly at the constant flow rate due to the flow resistance of a motor is determined by the flow resistance ratio(FRR), which is defined as a ratio of flow resistance of motor and the flow resistance of a constant throttling condition of a given point. The fan-motor assembly(S2 model) was modified to reduce the FRR from 9.0% to 2.4% and the experimental result shows that the efficiency of S2 model was improved by about 3% at best efficiency point.

The Effect of 12-Week Resistance Exercise on Muscle Loss and Metabolic Syndrome-Related Variables in Obese Elderly with Sarcopenia (12주 저항운동이 비만인 근감소증 노인의 근감소 및 대사증후군 관련 변인에 미치는 영향)

  • Shin, Jae-Suk;Kim, Hyun-Jun
    • Journal of The Korean Society of Integrative Medicine
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    • v.10 no.4
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    • pp.165-173
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    • 2022
  • Purpose : This study was conducted to apply a 12-week resistance exercise program to obese elderly people with sarcopenia and verify the risk factors of sarcopenia and metabolic syndrome as well as the effects of this program on improving muscle function, and thus to serve as basic data for preventing and improving sarcopenia. Methods : Forty elderly people aged 65 or older were recruited and underwent dual energy x-ray absorptiometry. Based on the criteria of appendicular skeletal muscle mass (ASM/Height2: less than 5.4 kg/m2) and body fat percentage (at least 30 % for women and 25 % for men), 18 obese elderly people with sarcopenia were finally selected after excluding 22 elderly people who did not meet the criteria. Variables related to sarcopenia, metabolic syndrome, and muscle function were measured before the 12-week resistance exercise program. Results : The 12-week resistance exercise program significantly increased the bone density and muscle mass and decreased the fat mass and fat percentage in obese elderly with sarcopenia. The 12-week resistance exercise program significantly increased the HDL-C and decreased the LDL-C and waist circumference in obese elderly people with sarcopenia (p<.05). The 12-week resistance exercise program significantly increase grip strength, static balance, and 6-minute walking in obese elderly people with sarcopenia (p<.05). Conclusion : Accordingly, resistance exercise is considered a way to reduce the exorbitant medical expenses of patients who are bedridden for long and improve the lowered quality of individuals in a super-aged society.

Surface erosion of MICP-treated sands: Erosion function apparatus tests and CFD-DEM bonding model

  • Soo-Min Ham;Min-Kyung Jeon;Tae-Hyuk Kwon
    • Geomechanics and Engineering
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    • v.33 no.2
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    • pp.133-140
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    • 2023
  • Soil erosion can cause scouring and failures of underwater structures, therefore, various soil improvement techniques are used to increase the soil erosion resistance. The microbially induced calcium carbonate precipitation (MICP) method is proposed to increase the erosion resistance, however, there are only limited experimental and numerical studies on the use of MICP treatment for improvement of surface erosion resistance. Therefore, this study investigates the improvement in surface erosion resistance of sands by MICP through laboratory experiments and numerical modeling. The surface erosion behaviors of coarse sands with various calcium carbonate contents were first investigated via the erosion function apparatus (EFA). The test results showed that MICP treatment increased the overall erosion resistance, and the contribution of the precipitated calcium carbonate to the erosion resistance and critical shear stress was quantified in relation to the calcium carbonate contents. Further, these surface erosion processes occurring in the EFA test were simulated through the coupled computational fluid dynamics (CFD) and discrete element method (DEM) with the cohesion bonding model to reflect the mineral precipitation effect. The simulation results were compared with the experimental results, and the developed CFD-DEM model with the cohesion bonding model well predicted the critical shear stress of MICP-treated sand. This work demonstrates that the MICP treatment is effective in improving soil erosion resistance, and the coupled CFD-DEM with a bonding model is a useful and promising tool to analyze the soil erosion behavior for MICP-treated sand at a particle scale.

A Double Mediating Effect of Resistance and Job Stress between Acceptance and Flourishing in Organizational Change (변화수용성과 번영 간 관계에서 변화저항과 직무스트레스의 이중 매개효과)

  • Lee, Hyun-joo;Jung, Sung-cheol
    • Journal of Venture Innovation
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    • v.4 no.3
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    • pp.89-108
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    • 2021
  • This study purposed to investigate the double mediating effect of the resistance and the job stress between the acceptance and the flourishing in organizational change. The survey has executed on 300 employees. The acceptance of organizational change was positively related to the flourishing, negatively to the resistance of organizational change and the job stress. The resistance of organizational change was positively related to the job stress, negatively to the flourishing. The job stress was negatively related to the flourishing. The resistance of organizational change was meditating the acceptance to change and the job stress, and the acceptance to change and the flourishing. The job stress on the acceptance to change and the flourishing partially mediated as well as the resistance to change and the flourishing. The resistance to change and the job stress double meditated the acceptance to change and the flourishing. The implications, limitations of this study for future research were discussed.

Native Pig and Chicken Breed Database: NPCDB

  • Jeong, Hyeon-Soo;Kim, Dae-Won;Chun, Se-Yoon;Sung, Samsun;Kim, Hyeon-Jeong;Cho, Seoae;Kim, Heebal;Oh, Sung-Jong
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.10
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    • pp.1394-1398
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    • 2014
  • Indigenous (native) breeds of livestock have higher disease resistance and adaptation to the environment due to high genetic diversity. Even though their extinction rate is accelerated due to the increase of commercial breeds, natural disaster, and civil war, there is a lack of well-established databases for the native breeds. Thus, we constructed the native pig and chicken breed database (NPCDB) which integrates available information on the breeds from around the world. It is a nonprofit public database aimed to provide information on the genetic resources of indigenous pig and chicken breeds for their conservation. The NPCDB (http://npcdb.snu.ac.kr/) provides the phenotypic information and population size of each breed as well as its specific habitat. In addition, it provides information on the distribution of genetic resources across the country. The database will contribute to understanding of the breed's characteristics such as disease resistance and adaptation to environmental changes as well as the conservation of indigenous genetic resources.

Relationship Between Microstructure and Electrical Properties of Glass-Ag Thick Film Conductors (유리-Ag계 후막도체의 미세구조와 전기특성과의 관계)

  • Lee, Byeong-Su;Hwang, Jun-Yeon
    • Korean Journal of Materials Research
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    • v.9 no.7
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    • pp.663-669
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    • 1999
  • Ag thick film conductors were fabricated with Ag powders having different degree of dispersion and glasses having different viscosity, and then the relationship between microstructure and electrical properties of the Ag films was investigated. Under the same processing condition. the Ag thick film that was prepared with the well-dispersed Ag powder and glass having low viscosity had denser microstructure and lower resistance than those of the film which was fabricated with the agglomerated Ag powder and glass having high viscosity It is believed to be due to the fast micro rearrangement kinetics when the well dispersed Ag powder was used, and all the microstructure development kinetics were impeded when low viscosity glass used. It was found that theme fast microstructure development kinetics resulted in the dense microstructure and the lower sheet resistance of the films. Also, the saturation time, at which at a given temperature no further decrease in the sheet resistance was obtained, was found to be shortened when well-dispersed Ag powder and low viscosity glass were used.

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A Study of Hydrogen Embrittlement Limit Potential of Cu-Containing High Strength Low Alloy Steel for Marine Structure by Potentiostatic SSRT Method (정전위 SSRT법에 의한 해양구조물용 Cu함유 고장력저합금강의 수소취성한계전위 규명에 관한 연구)

  • 김성종;박태원;심인옥;김종호;김영식;문경만
    • Journal of Welding and Joining
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    • v.19 no.2
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    • pp.182-190
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    • 2001
  • A marine structural material was well known to have high tensile strength, good weldability and proper corrosion resistance. Cu-containing high strength low alloy(HSLA) steel was recently developed for their purposes mentioned above. And the steel is free about preheating for welding, therefore it is reported that shipbuilding cost by using it can be saved more or less. However the marine structural materials like Cu-containing HSLA steel are being generally adopted with cathodic protection method in severe corrosive environment like natural sea water but the high strength steel may give rise to Hydrogen Embrittlement due to over protection at high cathodic current density for cathodic protection. In this study Cu-containing HSLA steel using well for marine atructure was investigated about the susceptibility of Hydrogen Embrittlement as functions of tensile strength, strain ratio, fracture time, and fracture mode, etc. and an optimum cathodic protection potential by slow strain rate test(SSRT) method as well as corrosion properties in natural sea water. And its corrosion resistance was superior to SS400 steel, but Hydrogen Embrittlement susceptibility of Cu-containing HSLA steel was higer than that of SS400 steel. However Hydrogen Embrittlement of its steel by SSRT method was showed with pheonomena such as decreasing of fracture time, strain ratio and fracture mode of QC(quasi-cleavage). Eventually it is suggested that an optimum cathodic protection potential not presenting Hydrogen Embrittlement of Cu-containing of HSLA steel by SSRT method was from-770mv(SCE) to - 900mV(SCE)under natural sea water.

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