• Animal Bioscience
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• 제34권4호
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• pp.680-691
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• 2021

Objective: This study was conducted to investigate the effect of fermented cottonseed meal (FCSM) on growth performance, carcass traits, and fat deposition in white-feather broiler chickens. Methods: A total of 480 male one-day-old white-feather broiler chickens were selected randomly and divided into four groups with six replicates of 20 chickens in each. The experimental chickens were fed diets including 3%, 6%, or 9% FCSM fermented by Candida tropicalis until 42 days old. In the experiment, the chickens of the control group were fed soybean meal. Results: FCSM supplementation linearly decreased the feed conversion ratio from d 15 to 21 and d 36 to 42, respectively (p<0.05). The percentage of carcass and semi-eviscerate increased in response to dietary FCSM supplementation at d 21 (p<0.05). The percentage of eviscerated and semi-eviscerate of 3FCSM was higher than that in other groups at d 35 (p<0.05). At the age of 42 d, the percentage of carcass increased in a quadratic way among increasing FCSM in diets (p<0.05). The subcutaneous fat thickness linearly decreased with the increasing levels of FCSM at d 21 (p<0.05). Gompertz and Logistic functions provided a better fit on abdominal fat and subcutaneous fat, respectively. The best fitted equation predicted that the maximum growth rate of abdominal fat weight and subcutaneous fat thickness occurred at d 28. FCSM had no significant effects on the shape of growth curve of abdominal fat weight and subcutaneous fat thickness, but reduced the height of the curve. Birds receiving the 6FCSM diet for 21 d had smaller adipocyte surface and lower serum glucose as well as triglyceride concentration. Conclusion: FCSM is beneficial for broiler chickens as it positively affects their growth and carcass in addition to altering their fat deposition.

• 한국콘텐츠학회논문지
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• 제21권12호
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• pp.423-435
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• 2021

본 연구의 목적은 중국 인터넷 동영상 특성이 사용자의 구독 만족도와 지속구독의도에 미치는 영향을 검증하는데 있다. 이를 위해 본 연구는 2021년 4월 16일부터 5월 16일까지 한 달 동안 구독한 경험이 있는 고객을 대상으로 온라인과 설문 조사를 실시하여 총338부 설문지에 수집하여 이에 대한 불성실한 응답과 구독하지 않은 응답 55부를 제외하고 총283부를 사용하여 실증분석을 실시하였다. 분석 결과는 다음과 같다. 첫째, 본 연구의 가설검증을 실시하기 전에 측정개념에 대한 타당성과 신뢰성 분석결과 인터넷 동영상 특성은 정확성, 사회적 영향, 서비스 품질, 콘텐츠 다양성, 정보성, 오락성 등 6개 요인으로 나타났다. 둘째, 인터넷 동영상의 특성 6가지 요인 중 정확성, 사회적 영향, 서비스 품질, 정보성이 구독 만족도의 유의한 정(+)영향을 미친 것이다. 셋째, 인터넷 동영상 특성 중의 콘텐츠 다양성과 오락성이 제외하고 나머지 4가지 요인들이 소비자의 지속구독의도에 긍정적이 영향을 미친 것으로 나타났다. 넷째, 구독만족도가 지속구독의도에 긍정적이 영향을 미친 것으로 검증하였다. 다섯째, 인터넷 동영상의 특성 및 지속구독의도 간의 구독만족도의 매개효과가 있다는 것으로 판단하였다.

• 수완나부미
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• 제4권1호
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• pp.57-90
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• 2012

This essay is for a study on the survey of buddhist cultural literary document about the Southeast Asia by Nanqishu(南齊書), Liangshu(梁書), Chiu T'angshu(舊唐書), Hsin T'ang shu(新唐書) which are included in the Chinese Official History and Jí-shénzhōu-sānbăo-găntōng-lù(集神州三寶感通錄), Weioshu Shilao Ji (魏書 釋老志). These documents allows us to imagine next some historical states. First, these documents are recording this area, especially Funan, as a plentiful diggings of gold, silver, tin, copper, etc. These are important materials for gilt bronze sculptures. Further, this local produced gold called 'Yangmai(楊邁)' is recognized as same as Zĭmòjīn(紫磨金) in china, and these documents explains the process of producing the bronze images and golden ornaments in Southeast Asia. Specially, this plentiful materials leads them to make a 10 wei(圍) tall golden-silver image which worshiped as hindu god or sometimes buddhist images. Second, Vietnam and Funan in Northern and Southern Dynasty periods were in antagonistic relationship not only in the political but also in religious between Vietnamese Hinduism and Funan's Buddhism. Under this situation, the monk Nagaxian(那伽仙) who had came from India was accredited to Southern Qi court as a delegate to build a good relationship by the common religion Buddhism. It means the Buddhism of Southeast Asia also took a role of diplomacy. Third, these documents proved the active Southeast Asian cultural exchange in early 3th century. At this time, Funan delegate Suwu(蘇物) visited the court of Kushan Dynasty in India and he is seemed to travel the city of Pātaliputra. It oppressed us to extend our outlook which have been restricted in the relationship between Southern india and Southeast Asia to more broaden area. In addition, the buddhist art of Southern India and Bodhgaya temple was imported to Southeast Asia directly to send to Southern China. For example, the wooden buddha image, Bodhgaya stupa image, and Sri Lanka style's buddha images are looked be introduced to Southern China at this time throughout the Southeast Asia. At last, we got to know that the court music of Kucha in the northern silk route was imported to the Southeast Asia in early middle age. Even it may be introduced by China, but this document is very important to make the surmise is not unreasonable that the buddhist culture of northern silk route imported to the Southeast Asia accompanied with Kucha music. The buddha images in Gandharan style which are excavated from some site of Southeast Asia may demonstrate this literary sources is authentic.

• Journal of Microbiology and Biotechnology
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• 제33권6호
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• pp.831-839
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• 2023

Tylosin is a potent veterinary macrolide antibiotic produced by the fermentation of Streptomyces fradiae; however, it is necessary to modify S. fradiae strains to improve tylosin production. In this study, we established a high-throughput, 24-well plate screening method for identifying S. fradiae strains that produce increased yields of tylosin. Additionally, we constructed mutant libraries of S. fradiae via ultraviolet (UV) irradiation and/or sodium nitrite mutagenesis. A primary screening of the libraries in 24-well plates and UV spectrophotometry identified S. fradiae mutants producing increased yields of tylosin. Mutants with tylosin yield 10% higher than the wild-type strain were inoculated into shake flasks, and the tylosin concentrations produced were determined by high-performance liquid chromatography (HPLC). Joint (UV irradiation and sodium nitrite) mutagenesis resulted in higher yields of mutants with enhanced tylosin production. Finally, 10 mutants showing higher tylosin yield were re-screened in shake flasks. The yield of tylosin A by strains UN-C183 (6767.64 ± 82.43 ㎍/ml) and UN-C137 (6889.72 ± 70.25 ㎍/ml) was significantly higher than that of the wild-type strain (6617.99 ± 22.67 ㎍/ml). These mutant strains will form the basis for further strain breeding in tylosin production.

• 한국컴퓨터정보학회논문지
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• 제28권4호
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• pp.133-142
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• 2023

국립공원은 세계 자연 보존 연맹(WWF)이 수립한 보호지역 관리 체계의 중요 유형 중 하나이며, 또한 자연 및 문화 유산의 효과적인 보호와 지속적인 이용을 실현하는 세계 각국의 관리 모델이다. 이러한 공원은 보호, 과학 연구, 교육, 레크리에이션 및 지역 개발을 비롯한 중요한 역할을 담당하다. 대용량 데이터의 배경 아래, 본 연구는 전 세계 연안 습지의 대표적인 대상인 중국 랴오하 국립공원을 사례 지역으로 삼아 파이썬 기술을 사용하여 중국의 주요 관광 OTA 사이트 중 하나인 망픈웨이 (Mafengwo), 셰어이(Gonglve), 큐난우(Chujingyou), 메이툰(Meituan) 및 대중점평넷(Dianping)의 관광객 여행기와 댓글을 데이터 소스로 수집하였다. 텍스트 시간 범위는 2015년부터 2022년까지이며, 총 2,998개의 댓글과 166,588개의 단어를 포함하다. ROST 콘텐츠 마이닝 및 Gephi 소프트웨어를 사용하여 랴오하 국립공원 방문객의 만족도, 인지 과정, 공선 네트워크, 감정 성향 등을 시각적 분석하였다. 결과는 다음과 같다. 야생 동물 및 식물 자원, 강과 바다가 결합 된 자연 경관, 습지 생태는 랴오하 국립공원 방문객의 인식에서 충분히 반영되었다. 방문객은 랴오하 국립공원에 대해 강한 긍정적인 감정을 가지고 있지만, 시설 서비스, 대중교육, 방문객 참여 경험 등에서 여전히 개선할 여지가 있다.

• Smart Structures and Systems
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• 제32권4호
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• pp.267-279
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• 2023

Data modelling and interpretation for structural health monitoring (SHM) field data are critical for evaluating structural performance and quantifying the vulnerability of infrastructure systems. In order to improve the data modelling accuracy, and extend the application range from data regression analysis to out-of-sample forecasting analysis, an improved most likely heteroscedastic Gaussian process (iMLHGP) methodology is proposed in this study by the incorporation of the outof-sample forecasting algorithm. The proposed iMLHGP method overcomes this limitation of constant variance of Gaussian process (GP), and can be used for estimating non-stationary typhoon-induced response statistics with high volatility. The first attempt at performing data regression and forecasting analysis on structural responses using the proposed iMLHGP method has been presented by applying it to real-world filed SHM data from an instrumented cable-stay bridge during typhoon events. Uncertainty quantification and correlation analysis were also carried out to investigate the influence of typhoons on bridge strain data. Results show that the iMLHGP method has high accuracy in both regression and out-of-sample forecasting. The iMLHGP framework takes both data heteroscedasticity and accurate analytical processing of noise variance (replace with a point estimation on the most likely value) into account to avoid the intensive computational effort. According to uncertainty quantification and correlation analysis results, the uncertainties of strain measurements are affected by both traffic and wind speed. The overall change of bridge strain is affected by temperature, and the local fluctuation is greatly affected by wind speed in typhoon conditions.

• Animal Bioscience
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• 제36권12호
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• pp.1775-1784
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• 2023

Objective: The aim of this study was to reveal the role and regulatory mechanism of miR-188-5p in the proliferation and differentiation of goat muscle satellite cells. Methods: Goat skeletal muscle satellite cells isolated in the pre-laboratory were used as the test material. First, the expression of miR-188-5p in goat muscle tissues at different developmental stages was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). In addition, miR-188-5p was transfected into goat skeletal muscle satellite cells by constructing mimics and inhibitors of miR-188-5p, respectively. The changes of differentiation marker gene expression were detected by qPCR method. Results: It was highly expressed in adult goat latissimus dorsi and leg muscles, goat fetal skeletal muscle, and at the differentiation stage of muscle satellite cells. Overexpression and interference of miR-188-5p showed that miR-188-5p inhibited the proliferation and promoted the differentiation of goat muscle satellite cells. Target gene prediction and dual luciferase assays showed that miR-188-5p could target the 3'untranslated region of the calcium/calmodulin dependent protein kinase II beta (CAMK2B) gene and inhibit luciferase activity. Further functional studies revealed that CAMK2B promoted the proliferation and inhibited the differentiation of goat muscle satellite cells, whereas si-CAMK2B restored the function of miR-188-5p inhibitor. Conclusion: These results suggest that miR-188-5p inhibits the proliferation and promotes the differentiation of goat muscle satellite cells by targeting CAMK2B. This study will provide a theoretical reference for future studies on the molecular mechanisms of skeletal muscle development in goats.

• Asian Pacific Journal of Cancer Prevention
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• 제15권21호
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• pp.9487-9494
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• 2014

Background: The size of a hepatic neoplasm is critical for staging, prognosis and selection of appropriate treatment. Our study aimed to compare the radiological size of solid hepatocellular carcinoma (HCC) masses on magnetic resonance imaging (MRI) with the pathological size in a Chinese population, and to elucidate discrepancies. Materials and Methods: A total of 178 consecutive patients diagnosed with HCC who underwent curative hepatic resection after enhanced MRI between July 2010 and October 2013 were retrospectively identified and analyzed. Pathological data of the whole removed tumors wereassessed and differences between radiological and pathological tumor size were identified. All patients were restaged using a modified Tumor-Node-Metastasis (TNM) staging system postoperatively according to the maximum diameter alteration. The lesions were classified as hypo-staged, iso-staged or hyper-staged for qualitative assessment. In the quantitative analysis, the relative pre and postoperative tumor size contrast ratio ($%{\Delta}size$) was also computed according to size intervals. In addition, the relationship between radiological and pathological tumor diameter variation and histologic grade was analyzed. Results: Pathological examination showed 85 (47.8%) patients were overestimated, 82 (46.1%) patients underestimated, while accurate measurement by MRI was found in 11 (6.2%) patients. Among the total subjects, 14 (7.9%) patients were hypo-staged and 15 (8.4%) were hyper-staged post-operatively. Accuracy of MRI for calculation and characterized staging was related to the lesion size, ranging from 83.1% to 87.4% (<2cm to ${\geq}5cm$, p=0.328) and from 62.5% to 89.1% (cT1 to cT4, p=0.006), respectively. Overall, MRI misjudged pathological size by 6.0 mm (p=0.588 ), and the greatest difference was observed in tumors <2cm (3.6 mm, $%{\Delta}size=16.9%$, p=0.028). No statistically significant difference was observed for moderately differentiated HCC (5.5mm, p=0.781). However, for well differentiated and poorly differentiated cases, radiographic tumor maximum diameter was significantly larger than the pathological maximum diameter by 3.15 mm and underestimated by 4.51 mm, respectively (p=0.034 and 0.020). Conclusions: A preoperative HCC tumor size measurement using MRI can provide relatively acceptable accuracy but may give rise to discrepancy in tumors in a certain size range or histologic grade. In pathological well differentiated subjects, the pathological tumor size was significantly overestimated, but underestimated in poorly differentiated HCC. The difference between radiological and pathological tumor size was greatest for tumors <2 cm. For some HCC patients, the size difference may have implications for the decision of resection, transplantation, ablation, or arterially directed therapy, and should be considered in staging or selecting the appropriate treatment tactics.

• Asian Pacific Journal of Cancer Prevention
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• 제15권14호
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• pp.5839-5843
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• 2014

Objective: The Zhejiang Provincial Cancer Prevention and Control Office collected cancer registration data during 2000 to 2009 from 6 cancer registries in Zhejiang province of China in order to analyze the cancer incidence. Methods: Descriptive analysis included cancer incidence stratified by sex, age and cancer site group. The proportions and cumulative rates of 10 common cancers in different groups were also calculated. Chinese population census in 1982 and Segi's population were used for calculating age-standardized incidence rates. The log-linear model was used for fitting to calculate the incidence trends. Results: The 6 cancer registries in Zhejiang province in China covered a total of 60,087,888 person-years during 2000 to 2009 (males 30,445,904, females 29,641,984). The total number of new cancer cases were 163,104 (males 92,982, females 70,122). The morphology verified cases accounted for 69.7%, and the new cases verified only by information from death certification accounted for 1.23%. The crude incidence rate in Zhejiang cancer registration areas was $271.5/10^5$ during 2000 to 2009 (male $305.41/10^5$, female $236.58/10^5$), age-standardized incidence rates by Chinese standard population (ASIRC) and by world standard population (ASIRW) were $147.1/10^5$ and $188.2/10^5$, the cumulative incidence rate (aged from 0 to 74) being 21.7%. The crude incidence rate was $209.6/10^5$ in 2000, and it increased to $320.20/10^5$ in 2009 (52.8%), with an annual percent change (APC) of 4.51% (95% confidence interval, 3.25%-5.79%). Age-specific incidence rate of 80-84 age group was achieved at the highest point of the incidence curve. Overall with different age groups, the cancer incidences differed, the incidence of liver cancer being highest in 15-44 age group in males; the incidence of breast cancer was the highest in 15-64 age group in females; the incidences of lung cancer were the highest in both males and females over the age of 65 years. Conclusions: Lung cancer, digestive system malignancies and breast cancer are the most common cancers in Zhejiang province in China requiring an especial focus. The incidences of thyroid cancer, prostate cancer, cervical cancer and lymphoma have increased rapidly. Prevention and control measures should be implemented for these cancers.

• 동국한의학연구소논문집
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• 제8권2호
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• pp.155-173
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• 2000

한의학의 진단(診斷)에는 망(望) 문(聞) 문(問) 절(切)의 사진법(四診法)과 여러 가지의 변증체계(辨證體系), 즉 기혈진액변증(氣血津液辨證) 장부변증(臟腑辨證), 육경변증(六經辨證), 위기영혈변증(衛氣營血辨證), 삼초변증(三焦辨證), 사상체질변증(四象體質辨證) 등이 응용되어 지고 있으며, 또한 그와 더불어 병상(症狀) 증후감병진단법(證候鑑別診斷法)등이 선택적으로 활용되어 지고 있다. 이러한 광범위(廣範位)한 진단방법(診斷方法)가운데 절진(切診)은 손가락 및 손바닥의 감각(感覺)을 운용(運用)해서 일정부위(一定部位)를 촉지(觸指), 접압(接壓)하는 검사방법(檢査方法)으로써 절맥진(切脈診)과 접진(接診)으로 크게 나눌 수 있다. 이중 안진(按診)이란 손을 사용하여 직접 환부에 촉모(觸摸) 안압(按壓)하여 이상변화를 알아내고 나아가서는 질병(疾病)의 부위(部位)와 성질(性質)과 병정(病情)의 경중(輕重) 등의 내부(內部)의 변화(變化)와 체표(體表)의 반응(反應)을 관찰(觀察)하여 중요(重要)한 변증자료(辨證資料)를 얻는 진단방법(診斷方法)의 한 종류(種類)를 말한다. 또한 접진(接診)에는 안기표(按肌表), 접수족(接手足), 안흉복(按胸腹), 접유혈진법(接兪穴診法)등을 들 수 있다. 배유혈(背兪穴)의 진단법(診斷法)은 경기(經綺)이라는 반응로(反應路)를 통(通)하여 체표(體表)에 발현(發現)되는 압통(壓痛), 자발통(自發痛), 긴장(緊張), 이완(弛緩), 경결(硬結) 및 조색상물(條索狀物) 등의 현상(現象)으로 부터 내부장기(內部臟器)의 병변(病變)을 진단(診斷)하는 방법(方法)이다. 이에 저자(著者)는 접진(接診)의 내용(內容)과 방법(方法)을 연구하면서 십이경맥(十二經脈)의 시동병(始動病) 소생병(所生病)을 알아보고 혈위진단(穴位診斷)의 방법(方法) 및 주의점(注意點)등을 아울러 정리하므로써 다음과 같은 결론(結論)을 얻었다. 1. 유혈(兪穴)은 각(各) 장부(臟腑)의 사기(邪氣)가 주입(注入)하는 곳으로 장병(臟病) 한증(寒症) 허증(虛症)의 의미를 내포한 음성병증(陰性病症) 치료(治療)에 중요(重要)한 곳이다. 2. 유차(兪次)의 촉진(觸診) 즉(卽) 모지(母指)로서 척추극돌기(脊椎棘突起) 좌우측(左右側)을 접압(接壓)하여서 상향(上向)이나 하향(下向)으로 추압지(推壓指)하면 극돌기(棘突起)의 돌(突), 함요(陷凹), 긴장(緊張), 이완(弛緩) 및 압통(壓痛)의 출현부위(出現部位)에 따라 계통별(系統別) 질환(疾患)을 판단(判斷)할 수 있다. 3. 실제(實際) 임상(臨床)에서 환자(患者)의 진단(診斷) 치료(治療)에 있어서 배부접진(背部接診)은 중요(重要)한 진단(診斷)의 한 영역(領域)으로 빠뜨리지 말고 꼭 참고(參考)하여야 할 것으로 사료(思料)된다. 4. 장부질환(臟腑疾患)에 대한 진단방법(診斷方法)의 다양화(多樣化)와 치료영역(治療領域)의 확대(擴大) 및 치료율(治療率)의 상승(上昇)을 위해 배부유혈(背部兪穴)의 정확(正確)한 인식(認識)과 유혈접진(兪穴接診)을 통하여 정확(正確)한 진단(診斷)이 되었으면 한다.