• Korean Journal of Microbiology
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• v.34 no.4
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• pp.188-193
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• 1998

The authors examined the variations of environmental factors, the distributions of cyanobacteria, heterotrophic bacteria, and microorganisms that inhibit the growth of Anabaena cylindrica according to development and extinction of cyanobacterial bloom at a site in Daechung Dam reservoir. And certified the relationship between each other. Water temperature variated in a typical pattern. pH and concentrations of dissolved oxygen and chlorophylla was high in bloom period, and lowered with the decline of bloom. Phosphorus played as a growth-limiting factor at this study site. Total nitrogen concentration increased during blooming period, which indicated that nitrogen has been fixed by aquatic organisms such as cyanobacteria. Cyanobacteria distributed from June 17, and such cyanobacterial species as Anabaena spp., Aphanizomenon spp., Microcystis spp., Oscillatoria spp. and Phormidium spp. was detected during study period. Anabaena spp. distributed relatively highly distributed from July 23 to September 22, and disappeared completely at September 29. Heterotrophic bacterial and cyanobacterial populations varied inverse-proportionally. There was a relevancy between the variations of Anabaena spp., heterotrophic bacteria, and microorganisms that inhibit the growth of Anabaena cylindrica. Microorganisms that inhibit the growth of Anabaena cylindrica distributed from early growth phase of Anabaena spp. population to immediately after the extinction of Anabaena spp. With the population of Anabaena cylindrica growth-inhibiting microorganisms decreasing, increases of heterotrophic bacterial population followed it. Thease results indicate that microorganisms have a part in the extinction of cyanobacterial bloom, especially at its destroying period.

• Journal of environmental and Sanitary engineering
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• v.24 no.2
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• pp.40-48
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• 2009

Unusual bloom of toxic cyanobacteria in water bodies have drawn attention of environmentalists world over. Major bloom of Anabaena, Microcystis in water storage reservoir, rivers and lake leading to adverse health effects have been reported from Australia, England and many part of the world. These cyanobacterial cells can release intercellular matter like toxin in water and these intercellular matter can increase the concentration of organic matter. Cellysis can occur when algal cells meet the disinfectants like chlorine in water treatment plant and the resultant rising up of DOC(Dissolved Organic Carbon) or TOC(Total Organic Carbon) can increase the formation of disinfection by products. Disinfectants that kill microorganisms react with the organic or inorganic matter in raw water. In general disinfectants oxidize the matter in raw water and the resultant products can be harmful to human. There are always conflict about which is more important, disinfection or minimizing disinfection by products. The best treatment process for raw water is the process of the lowest disinfection by products and also the the lowest microorganism. In this study the cultured cells, Microcytis Aeruginosa(MA), Anabaena Flos-aquae(AF), Anabaena Cylindrica(AC), and the cells obtained in Daechung Dam(DC) whose dominant species was Anabaena Cylindrica were subjected to chlorination. Chlorination oxidizes inorganic and organic compounds and destruct live cells in raw water. Chloroform was analyzed for the cultured cells which were treated with $20mg/\ell$ dose of chlorine. In general chloroform is easily formed when dissolved organic matter react with chlorine. The cultured cells contributes the concentration of dissolved organic carbon and also that of total organic carbon which might be potent precusors of chloroform formed. The correlations of the concentration of chloroform, DOC and TOC were investigate in this study.

• Korean Journal of Microbiology
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• v.40 no.2
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• pp.115-120
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• 2004

A Gram (-), rod-shaped bacterium in size of 1.3∼$1.8{\times}0.35{\mu}m$ inhibiting the growth of cyanobacterium (Ana-baena cylindrica) was isolated and designated NG-2 in this manuscript. This isolate showed positive reactions for catalase and oxidase, and optimal growth conditions of 35∼TEX>$40<^{\circ}C$ and pH 9.0. In a mixed-culture of A. cylindrica and the isolate, each microorganism grew inverse-proportionally, and the cyanobacterial vegetative cells almost completely disappeared within 24 hours. NG-2 lysed A. cylindrica only under light, which means that lytic activity of NG-2 was dependent on the photosynthetic activity of host. When observed under phase contrast microscope, the isolate lysed vegetative cells of A. cylindrica in scattered state in a liquid medium, whereas het-erocysts have not been lysed. When cyanobacterial cell walls have been lysed partly, NG-2 attatched around A. cylindrica filament and formed colony, then encouraged complete lysis of cyanobacterial cells. The isolate showed similar lytic activity in natural water as in an artificial medium. And lytic activity of NG-2 was enhanced when attached on expandable polystyrene bead.

• Korean Journal of Ecology and Environment
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• v.37 no.2 s.107
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• pp.241-247
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• 2004

Isolation and identification of alga-lytic bacteria were carried out. Fifteen isolates of alga-lytic bacteria were screened by the double layer method using A. cylindrica NIES-19 as a sole nutrient and four isolates among them were compared with their alga-lytic activity. The isolate AK-05 exhibiting the highest alga-lytic activity was identified as Acidovorax temperans base on its 16S rDNA sequence. The culture supernatant of the isolate AK-05 was reliable for the alga-lytic. Alga-lytic activity assays of culture supernatant revealed that the major substances for alga-lytic activity were non-proteins and heat stable. The highest alga-Iytic activity was practical under alkaline conditions and at 25${\sim}$$30^{\circ}C$. It is indicating an advantage for the application of water blooms by cyanobacteria in eutrophic lakes where the pH is generally in alkaline region.

• Korean Journal of Ecology and Environment
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• v.33 no.2 s.90
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• pp.128-135
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• 2000

The authors surveyed the seasonal variations of environmental factors, the distributions of heterotrophic bacteria and Anabaena cylindrica growth-inhibiting bacteria at each water layer in Daechung Reservoir to verify the role of bacteria during the extinction of bloom. Average water depth at site 1, 2, and 3 were 25.5 m, 15.0 m and 12.3 m, respectively. Water temperature showed a typical pattern seasonally. The variation of DO was relatively inverse proportional to that of water temperature, although it was irregular during summer time. DO decreased gradually to early May, fluctuated sharply after then, and followed by gradual increasement after middle of September. This variation pattern was notable at surface layer. There was remarkable difference in DO concentraion between surface layer and the other water layers during the period in which DO irregulary varied. The variation range of chlorophyll-a concentraion at surface layer in summer time was broad, and it was relatively high when DO was high. The population size of heterotrophic bacteria was high from Spring to Autumn, an declined after September when the water temperature droped rapidly. Especially this variation pattern was prominent at the surface layer. Bacteria that inhibit the growth of A. cylindrica was almost not detected by June, and its distribution increased in July. Afterward, it showed different variation pattern between each site. The distribution of A. cylindrica growth-inhibiting bacteria was higher at the middle and bottom layer than the surface layer in July and October, when it was larger at all sites for the study period. This result suggests that the antagonistic bacteria exhibit higher activity when host activity drops. These results also suggest that natural water bacteria control the distirbution of cyanobacteria, especially its activity as controller is remarkable when cyanobacterial growth declines.