• 제목/요약/키워드: Water temperature stress

검색결과 643건 처리시간 0.029초

명태(Gadus chalcogrammus)의 일차 간세포 배양에서 온도 스트레스에 따른 HSP70 mRNA와 단백질 발현 (Expression of HSP70 mRNA and Protein based on the Thermal Stress in the Primary Hepatocyte Culture of Walleye Pollock (Gadus chalcogrammus))

  • 김소선;이창주;박장수
    • 한국환경과학회지
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    • 제29권6호
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    • pp.633-641
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    • 2020
  • Water temperature is one of the most important factors of fish survival, affecting the habitat, migration route, development, and reproduction. This experiment studied the induction level of heat shock protein (HSP70) mRNA and protein in a walleye pollock (Gadus chalcogrammus) primary hepatocyte culture based on different temperatures. Hepatocytes were attached at 7.5℃ for 24 hours. Hsp70 induction levels were then measured for 48 hours at 5, 8, 11, 14, and 17℃. The induction level was lowest at 5℃ and generally increased with temperature until 14℃. The induction level was reduced at 17℃, indicating that 14℃ is the highest tolerable temperature for hepatocytes. These data indicate that primary hepatocyte cell culture is under no stress at 5 and 8℃. Temperatures greater than 11℃ induce stress, showing similar induction patterns in both mRNA and protein in hepatocytes. The results suggest that 14℃ is the maximum internal defense temperature of walleye pollock survival.

산란계에 적용 가능한 공기-물 히트펌프의 음용수 공급시스템 이용기술에 관한 연구 (Study on the Utilization of Drinking Water Supply System of Air-water Heat Pumps Applicable to Laying Hen)

  • 백이;강석원;장재경;권진경
    • 한국기계기술학회지
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    • 제20권6호
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    • pp.917-923
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    • 2018
  • The drinking water supply system applicable to the laying hen consists of air-water heat pumps, drinking water tanks, heat stroage tank, circulation pumps, PE pipes, nipples, and control panels. When the heat pump system has power of 7.7 to 8.7 kW per hour, the performance coefficient is between 3.1 and 3.5. The supply temperature from the heat pump to the heat stroage tank was stabilized at about $12{\pm}1^{\circ}C$, but the return temperature showed a variation of from 8 to $14^{\circ}C$. Stratified temperature in the storage tank appeared at $12.^{\circ}C$, $13.5^{\circ}C$ and $14.4^{\circ}C$, respectively. The drinking water supply temperature remained set at $15^{\circ}C$ and $25^{\circ}C$, and the conventional tap water showed a variation for $23^{\circ}C$ to $30^{\circ}C$. As chickens grow older, the amount of food intake and drinking water increased. $y=-0.0563x^2+4.7383x+8.743$, $R^2=0.98$ and the feed intake showed $y=-0.1013x^2+8.5611x$. In the future, further studies will need to figure out the cooling effect on heat stress of livestock.

큐폴라 용해로 철피의 열응력 및 피로수명 해석 (Analysis of Thermal Stress and Fatigue Life in the Steel Shell of a Cupola Furnace)

  • 양영수;배강열
    • 한국기계가공학회지
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    • 제19권2호
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    • pp.47-54
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    • 2020
  • Themo-mechanical analysis was carried out using the finite element method for the steel shell of a cupola furnace. When the outer surface of the shell was cooled with water to within the temperature range of 35-80 ℃ during operation of the cupola, the inner surface of the shell was expected to exhibit a temperature of 65-248 ℃ based on heat transfer analysis. The shell was also expected to have an equivalent stress range of 100-280 MPa in the outer surface over the temperature range examined. Upon cooling the shell to obtain an outer surface temperature <80 ℃, the maximum equivalent stress of the shell did not exceed the yield strength. Although the temperature of the outer surface varied between 35 and 80 ℃ periodically due to the cooling control problem, the fatigue stress at the outer surface of the shell was calculated to be within the fatigue strength. During a non-operational period to examine the system between furnace operations, the thermal stress presented in the shell was sufficiently low to reach the desired yield strength and fatigue limit.

콘크리트의 부등건조수축으로 인한 응력의 해석 (Stress Analysis for Differential Drying Shrinkage of Concrete)

  • 김진근;김효범
    • 한국전산구조공학회:학술대회논문집
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    • 한국전산구조공학회 1994년도 봄 학술발표회 논문집
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    • pp.155-162
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    • 1994
  • The drying shrinkage of concrete has a close relation to the water movement, Since the diffusion process of water in concrete is strongly dependent on the temperature and pore humidity, the process is highly nonlinear phenomena. It is shown that the analytical results of this study are in good agreement with experimental data in the literatures, and results calculated by BP-KX model. The internal stress caused by moisture distribution which was resulted from the diffusion process, was calculated quantitatively. The tensile stress which occurred in the drying outer zone mostly exceeded the tensile strength of concrete, and necessarily would result in crack formation.

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인삼의 수분생리 1. 자생지관찰.재배 경험.기상요인과 근 및 엽의 특성 (Water Physiology of Panax ginseng. 1. Habitat observation. cultural experience, weather factors and characteristics of root and leaf)

  • 박훈
    • Journal of Ginseng Research
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    • 제4권2호
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    • pp.197-221
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    • 1980
  • Habitat observation, cultural experience of old and present plantation, weather factors in relation to crop stand and water physiology of root and leaf were reviewed. According to habitat observation ginseng plants love water but plate wit talus well grow at drained place with high moisture content in air and soil while ginseng plants were not found in dry or wet place. According to cultivation experience ginseng plants require abundant water in nursery and main field but most old planters believe that ginseng plaints are draught-loving thus require little water. The experience that rain especially in summer i.e unfavorable might be due to mechanical damage of leaves arid leaf disease infection, or severe leaf fall which is caused by high air temperature and coinsided with rain. According to crop stand observation in relation to weather factors abunsant water increased each root weight but decreased total yield indicating tile increase of missing root rate. Rain in summer was unfavorable too. Though rain in June was favorable for high yield general experience that cloudy day and rain were unfavorable might be due to low light intensity under shade. Present leading planters also do loot consider the importance of water in main field. Water content is higher in top than in root and highest in central portion of root and in stem of top. For seedling the heavier the weight of root is tile higher the water content while it reveries from two years old. Water potential of intact root appeared to be -2.89 bar suggesting high sensitivity to water environment. Under water stress water content severly decreased only in leaf. Water content of leaf appeared to be 78% for optimum, below 72% for functional damage and 68% for perm anent wilting. Transpiration or curs Principally through stomata in lower side of leaf thus contribution of upper side transpiration decreased with the increase of intensity. Transpiration is greater in the leaves grown under high light intensity. Thus water content is lower with high light inte nsity under field condition indicating that light is probable cause of water stress in field. Transpiration reached maximum at 10K1ut The decrease of transpiration at higher temperature seems to be due to the decrease of stomata aperture caused by water stress. Severe decrease of photosynthesis under water stress seems to be principally due to functional damage which is not caused by high temperature and Partly due to poor CO2 supply. Water potential of leaf appeared to be -16.8 bar suggesting weakness in draught tolerance. Ginseng leaves absorb water under high humidity. Water free space of leaf disc is %mailer than that of soybean leaf and water uptake appears to be more than two steps.

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작물 가뭄스트레스 조기탐지 가능성 타진을 위한 서로 다른 종류의 식생지수 활용 (Possibility for Early Detection on Crop Water Stress Using Plural Vegetation Indices)

  • 문현동;조은이;조유나;김현기;김보경;이유현;정회정;권동원;조재일
    • 대한원격탐사학회지
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    • 제38권6_1호
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    • pp.1573-1579
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    • 2022
  • 기후변화에 따른 우리나라의 빈번한 가뭄에 따라 작물 생산량은 보존하면서 수자원 관리를 극대화하는 방법으로 작물 가뭄스트레스를 조기 탐지하여 적기에 적량이 관수되는 방식이 최근 주목받고 있다. 하지만, 대부분의 선행연구들은 가뭄스트레스가 상당히 진행되어 표징이 뚜렷한 시기에 실험되고 있어 가뭄 스트레스 초기에 원격탐지 될 수 있는 생리적 특징이 많이 알려지지 않았다. 본 연구에서는 콩을 대상으로 초기 수분 스트레스 시기에 열화상 카메라와 초분광 카메라 촬영을 수행하였다. 스트레스 우세 시기 작물 대상의 선행연구들 결과와 마찬가지로 엽온은 높아졌으며, 엽록소 함량을 대표하는 MERIS Terrestrial Chlorophyll Index (MTCI)는 감소하였다. 하지만, 오히려 광합성 능력을 나타내는 Chlorophyll/Carotenoid Index (CCI)가 증가하는 경향이 나타났다. 이는 스트레스 우세 상태와 마찬가지로 가뭄스트레스 초기에도 엽록소와 카로티노이드(carotenoid) 감소가 동시에 일어났지만, 처음의 카로티노이드 감소 속도가 엽록소에 비해 컸기 때문에 일시적으로 엽록소에 대한 카로티노이드 비율이 낮아져서 CCI가 높아진 것으로 사료된다. 이러한 스트레스 초기 반응은 조기탐지 알고리즘 개발에 유용할 것으로 기대한다.

Study on Leak Rate of SCC Degraded Alloy 600 Tubings of PWRs

  • Hwang, Seong Sik;Kim, Joung Soo;Kasza, Ken E.;Park, Jangyul
    • Corrosion Science and Technology
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    • 제3권6호
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    • pp.233-239
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    • 2004
  • Primary water stress corrosion cracking of steam generator tubings occur on many tubes in pressurized water reactors(PWRs), and they are repaired using sleeves or plugs. In order to develop proper repair criteria, it is necessary to know the leak behavior of the tubes, which have stress corrosion cracks. Crack development tests were carried out on the tubes at room temperature, and leak rate and burst pressure were measured on the degraded tubes at room temperature and a high temperature. No leakage was detected on the tubes where 100 % through wall crack developed, at 1560 psi, which is an operating pressure difference of pressurized water reactors(PWRs). In some tests, leak rates of the tubes increased with time at a constant internal water pressure. A test tube showed a very small amount of leakage at 2700 psi in a high temperature pressure test at $282^{\circ}C$, but it disappeared after the pressure increased slightly. Even cracks are 100 % through wall, they need to open in order to reach a certain amount of leak rate at the operating pressure difference.

사육수의 고수온 스트레스가 벤자리(Parapristipoma trilineatum)에 미치는 생리학적 영향 (Physiological Responses of the Chicken Grunt Parapristipoma trilineatum to High Water Temperature Stress)

  • 김기혁;홍성원;문혜나;여인규
    • 한국수산과학회지
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    • 제51권6호
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    • pp.714-719
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    • 2018
  • We investigated the effects of water temperature on physiological parameters in the chicken grunt Parapristipoma trilineatum. At high temperature, the aspartate aminotransferase (AST) and the alanine aminotransferase (ALT) levels were increased, suggesting that high temperature induced hepatic damage. In addition, total protein (TP) was high at high water temperatures, which were considered stressful in the breeding environment. At high water temperatures, triglycerides (TG) were low due to increased metabolic activity, which decreased the blood TG levels as TG were used as an energy source. There was no significant difference in the plasma osmolality or the blood ion concentrations with water temperature. In generally, lysozyme, a factor in innate immunity, increased with water temperature. However, lysozyme activity tended to decrease with increasing water temperature, but the difference was not significant. These results suggested that the decrease of biophylaxis at high temperature was affect the growth or survival of the population.

Combined Effects of Copper and Temperature on Antioxidant Enzymes in the Black Rockfish Sebastes schlegeli

  • Min, Eun Young;Baeck, Su Kyong;Kang, Ju-Chan
    • Fisheries and Aquatic Sciences
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    • 제17권3호
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    • pp.345-353
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    • 2014
  • Copper has been widely used to control algae and pathogens in fish culture ponds. However, its toxic effects on fish depend not only on its concentration in the water but also on the water quality. A laboratory experiment was conducted to assess copper toxicity in the black rockfish Sebastes schlegeli using a panel of antioxidant enzymes, including glutathione (GSH), glutathione S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR) and superoxide dismutase (SOD), at different levels of copper at three water temperatures (WT, 18, 23, $28^{\circ}C$) for 4 days. After exposure to two copper concentrations (100 and $200{\mu}g/L$), GSH levels and GST activities increased significantly, depending on WT (P < 0.05) in the liver, gill, and kidney of the black rockfish. GPx and SOD activities decreased significantly with both increasing WT and copper treatment in the organs of black rockfish (P < 0.05). These changes can be seen as initial responses to temperature stress and as a sustained response to copper exposure. This also indicates that GSH and related enzymes activities were sensitive indexes to stress by toxicants such as copper. The present findings suggest that simultaneous stress due to temperature change and copper exposure can accelerate changes in enzymes activities in the black rockfish. This provides another example of synergism between environmental temperature and pollutants, which may have important implications for the survival of fish in polluted environments during seasonal warming and/or global climate change.

Thermal Effects on Bioaccumulation and Antioxidant Enzyme Response in the Liver and Gills of Tilapia Oreochromis niloticus Exposed to Arsenic

  • Min, EunYoung;Jeong, Ji Won;Kang, Ju-Chan
    • Fisheries and Aquatic Sciences
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    • 제18권2호
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    • pp.195-202
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    • 2015
  • The effects of waterborne arsenic (As) exposure on bioaccumulation and antioxidant defenses were examined in the liver and gills of tilapia, Oreochromis niloticus, under thermal stress. Tilapia were exposed to different As concentrations (0, 200, and $400{\mu}g\;L^{-1}$) at three water temperatures (20, 25, and $30^{\circ}C$) for 20 days. After As exposure, higher levels of As accumulation were observed in the gills compared with the liver in elevated water temperatures. In terms of the antioxidant response, glutathione (GSH) levels and the activities of glutathione reductase (GR), glutathione peroxidase (GPx), and glutathione S-transferase (GST) significantly decreased in the liver and gills of tilapia exposed to As for 20 days, regardless of the As concentration (200 and $400{\mu}g\;L^{-1}$) or water temperature. These enzyme activities exhibited greater fluctuations in the liver and gills of tilapia after As exposure in water warmer than $20^{\circ}C$. The present findings suggest that the simultaneous stress of temperature change and As exposure can accelerate As accumulation and alter the antioxidant enzymes activities of tilapia.