• Journal of Plant Biotechnology
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• 제8권1호
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• pp.21-25
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• 2006

Phragmites australis (reed) has received much attention as being one of the principle emergent aquatic plants for treating industrial and civil wastewater. Plant regeneration via plant tissue culture in p. australis was investigated. Three types of callus were identified from seeds on N6 medium plus 4.5 UM 2,4-dichlorophenoxyacetic acid (2,4-D). Yellow compact type showed the best redifferentiation, whereas white compact type and yellow friable were not competent to differentiate into plane. Solid medium culture was better than liquid suspension culture for enhancing callus growth when N6 medium supplemented with 4.5 ${\mu}M$ 2,4-D was used. Phytagel, as a gelling agent, was superior to agar in plant regeneration on N6 medium, supplemented with 9.4 ${\mu}M$ kinetin and 0.54 ${\mu}M$ $\alpha$-naphthaleneacetic acid (NAA). Transfer of the plantlets regenerated from kinetin and NAA-supplemented N6 medium to growth regulator-free MS medium enhanced the further development of the plantlets. Plantlets on subsequently grown to maturity when tansferred to potting soil. The regenerated plants exhibited morphologically normal. The system for plant regeneration of P. australis enables to propagate elite lines on a large scale for water purification in the ecosystem

• 한국결정성장학회지
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• 제33권3호
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• pp.104-109
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• 2023

Electrospinning was performed using an eco-friendly solvent composed of acetic acid, ethyl acetate and distilled water to investigate the effect of gelatin concentration on mechanical properties and cytotoxicity of absorbable poly(D,L-lactic acid) (PDLLA)/gelatin blend membrane. The tensile stress, strain at break, and WUC of the PDLLA/gelatin (97/3) scaffold at 26 wt% concentration were determined to be 3.9 ± 0.7 MPa, 37 ± 1.3 %, and 273 ± 33 %, respectively. FT-IR results revealed that PDLLA and gelatin were bound only by van der Waals interactions. The cell viability of PDLLA/gelatin membranes containing 0 %, 1 %, 2 %, 3 %, and 4 % gelatin were more than 100 %, which makes all membranes highly suitable as a barrier membrane for absorbable periodontal tissue regeneration due to their marketed physical properties and biocompatibility.

• 한국자원식물학회지
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• 제30권6호
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• pp.612-617
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• 2017

The preservation of pear germplasm, like that of other clonal germplasms, is difficult because it requires conservation of whole plants or their tissues. Among the currently available methods for long-term conservation of clonal germplasm, cryopreservation of shoot tips is the most reliable and cost- and space-effective option. Alginate-coated axillary shoot tips from in vitro-grown pear were conserved successfully in liquid nitrogen (LN) following dehydration. Shoot recovery from cryopreserved shoot tips was improved greatly after 8 weeks of cold acclimation, but recovery decreased slightly after then. The highest regeneration rate was observed when in vitro shoot tips were preincubated in MS (Murashige and Skoog) medium with 0.3 M sucrose for 48 h, and when alginate-coated shoot tips were precultured in MS medium with increasing sucrose concentrations (0.5 M and 0.7 M) for 8 and 16 h, respectively. When the encapsulated beads were dehydrated for up to 7 h [25% water content (fresh weight basis)] under laminar flow, the highest regeneration rate was observed in "BaeYun No. 3" (55.7%) and "Whanggeum" (43.3%) after warming from LN. This technique is useful as a practical procedure to cryopreserve plant material that is sensitive to freezing of the surrounding cryoprotectant medium. Therefore, this technique appears to be promising for the cryopreservation of shoot tips from in vitro-grown plantlets of pear germplasm.

• 한국해양환경ㆍ에너지학회지
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• 제17권2호
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• pp.116-121
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• 2014

지열수를 온열원으로 사용하고, 해양심층수를 열침으로 사용하는 바이너리(binary) 지열 발전시스템은 기존 지열 발전시스템의 효율을 증대하기 위한 재열과정과 터빈출력을 향상시키기 위한 다단과정을 각각 또는 복합적으로 적용하여 다단재열재생사이클의 성능개선을 검토하였다. 사이클종류는 다단재열사이클(Multi Stage reheater cycle; MS), 다단재열재생사이클(Multi stage reheater regeneration cycle; MSR)이 있다. 작동유체는 R134a, R245fa를 적용하였으며 온열원의 온도가 $65^{\circ}C$, $75^{\circ}C$, $85^{\circ}C$ 열침은 $5^{\circ}C$를 적용하여 기본해석을 수행하였다. 본 논문에서는 온열원변화, 작동유체의 종류, 사이클의 종류에 따른 해양지열발전용 다단재열재생사이클의 출력 및 효율을 높이기 위한 해석을 수행하였다. 이를 열역학적 사이클로 모사하기 위한 상용 프로그램인 Aspen HYSYS(V8.0)를 이용하여 해석을 진행 하였다. 작동유체는 R245fa가 R134a보다 우수한 성능을 보였으며, 온열원의 변화와 각각의 사이클 종류에 따라 적절한 작동유체가 있음을 확인 할 수 있었다. 사이클의 출력 및 효율은 각각 MS사이클과 MSR사이클에서 좋은 성능을 나타냈다.

• 한국수자원학회논문집
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• 제46권8호
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• pp.843-855
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• 2013

최근 과학기술이 고도로 발달되어 있는 현대사회에서 자연재해에 대한 지속적인 대비와 예측이 진행되고 있는 가운데 인간은 자연재해를 극복하기 위하여 오랜기간 꾸준하게 노력을 시도하고 있다. 그러나 자연재해의 피해 또한 인간의 노력 이상으로 커지고 있으며 자연재해를 예방하기 위해서 자연재해위험지구 정비사업을 수행하고 있다. 그러나 정비사업 결과에 대한 구체적인 효과분석이 미비한 상태이다. 본 연구에서는 자연재해위험지구 정비사업의 효과를 분석하기 위하여 분석대상지구를 선정하고 지역주민 현장설문조사를 실시하여 설문도구의 타당성과 신뢰도 분석을 실시하고 빈도 분석 및 기술통계 분석을 실시하였다. 또한 구조방정식모형(AMOS)을 통하여 정책의 타당성을 분석하였다. 분석결과 자연재해정비사업의 정책은 합리성과 적합성이 담보되어 있고, 지역주민의 정신적 물리적 환경을 개선시키는 것으로 나타났다. 또한 정비사업에 대하여 긍정적인 인식을 형성하였으며, 정부의 정치적 지지도까지 향상시키는 것으로 나타났다. 따라서 자연재해정비사업의 진행은 합리성과 적합성이 담보된 사업이고 이는 지역사회를 발전시키고 건전한 민주주의 발전까지 영향을 미치는 것으로 나타났다.

• Biomolecules & Therapeutics
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• 제23권1호
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• pp.90-97
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• 2015

Water chestnut (Trapa japonica Flerov.) is an annual aquatic plant. In the present study, we showed that the treatment of water chestnut extracted with boiling water resulted in a significant increase 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and decrease the intracellular $H_2O_2$-induced accumulation of reactive oxygen species. In addition, water chestnut extract (WCE) inhibited lipopolysaccharide (LPS)-induced nitric oxide production and suppressed mRNA and protein expression of the inducible nitric oxide synthase gene. The cytokine array results showed that WCE inhibited inflammatory cytokine secretion. Also, WCE reduced tumor necrosis factor-${\alpha}$- and interleukin-6-induced nuclear factor-${\kappa}B$ activity. Furthermore, during sodium lauryl sulfate (SLS)-induced irritation of human skin, WCE reduced SLS-induced skin erythema and improved barrier regeneration. These results indicate that WCE may be a promising topical anti-inflammatory agent.

• 한국물환경학회지
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• 제34권1호
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• pp.57-66
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• 2018

Water disasters such as flash floods and inundation caused by localized heavy rainfall in urban areas have a large impact on climate change but are also closely related to the increase in impervious areas as pointed out in domestic and international studies. It is difficult to secure natural green areas in urban areas that have already been developed. So, urban regeneration can be expected using water management optimized with technologies to secure infiltration and storage capacity such as Low-Impact Development technology. In this study, the water cycle improvement ability was confirmed by applying the LID technology within the district unit plan of the environmentally friendly village, and the economic feasibility of LID application was analyzed by estimating the costs and benefits of installing the facilities. The site was planned to conserve sufficient green and plans for securing the watershed infiltration and storage capacity were formulated with the application of additional LID technology, such as infiltration trenches, rain barrels and permeable pavements. The LID design method applicable to the site was established, and the water balance of the watershed was analyzed through simulations of the SWMM model. The water circulation improvement effect was confirmed through the water balance analysis, and the cost-benefits were determined according to the estimation method, and the economic analysis was conducted. This study confirms that the investment of LID technology is economically feasible for the hydrological improvement effect of the housing complex.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.250.2-250.2
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• 2014

Currently, teeth whitening method which is applicable to dental surgery is that physician expertises give medical treatment to teeth directly dealed with a high concentration of hydrogen peroxide and carbamide peroxide. If hydrogen peroxide concentration is too high for treatment of maximized teeth whitening effect [1], it is harmful to the human body [2]. To the maximum effective and no harmful teeth whitening effect in a short period of time at home, we have observed the whitening effect using carbamide peroxide (15%) and a low-temperature atmospheric pressure plasma jet which is regulated by the Food and Drug Administration. The gas supplied conditions of the non-thermal atmospheric pressure plasma jet was with the humidified (0.6%) gas in nitrogen or air at gas flow rate of 1000 sccm. Also, the measurement of chemical species from the jet was carried out using the optical emission spectroscopy (OES), the evidence of increased reactive oxygen species compared to non-humidified plasma jet. We have found that the whitening effect of the plasma is very excellent through this experiment, when bovine teeth are treated in carbamide peroxide (15%) and water vapor (0.2 to 1%). The brightness of whitening teeth was increased up to 2 times longer in the CIE chromaticity coordinates. The colorimetric spectrometer (CM-3500d) can measure color degree of whitening effect.

• Korean Chemical Engineering Research
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• 제47권3호
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• pp.349-354
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• 2009

본 연구에서는 전력연구원으로부터 공급받은 K-계열 건식흡수제를 이용하여 회분식 기포유동층 반응기에서 흡수-재생 반복실험을 통한 $H_2O$ 주입농도 및 재생온도에 따른 반응 특성을 살펴보았다. K-계열 건식흡수제는 $CO_2$ 흡수를 위한 탄산칼륨과 내마모성과 기계적 강도를 위한 지지체로 구성되어 있다. 흡수반응과 재생반응 특성을 살펴보기 위해 처음 한 시간 동안 흡수반응을 수행하고 다음 한 시간 동안 재생반응을 수행하는 과정을 3차례 반복하여 실험하였다. $H_2O$ 농도의 영향을 파악하기 위해서 흡수반응은 $70^{\circ}C$에서 $H_2O$ 농도를 7.3, 12.2, 19.7, 30.8%로 변화하여 실험을 수행하였으며 재생반응은 $N_2$ 기체를 유동화기체로 사용하여 $150^{\circ}C$에서 수행하였다. 재생온도의 영향을 파악하기 위해서는 흡수반응에서의 $H_2O$ 농도를 12.2%에 고정한 상태에서 재생온도를 150, 200, 300, $400^{\circ}C$로 변화하여 실험을 수행하였다. 수분 함량이 $1.97\;mol\;H_2O/mol\;CO_2$인 경우 흡수반응에서 흡수율이 가장 우수함을 확인하였다. 또한 재생온도가 $400^{\circ}C$에서 가장 높은 재생율을 보이는 것을 확인하였다. 재생온도가 $150^{\circ}C$에서 재생율은 대략 60% 정도였으며 실제 두개의 유동층 반응기를 가진 연속장치의 경우 부분적인 재생을 유지하면서 운전이 수행되기 때문에 재생온도는 $150^{\circ}C$ 이상이면 적절하다고 판단된다. 실제 연속운전에서는 적절한 고체순환량을 결정하는 고체이용율과 재생에너지를 결정하는 재생온도 사이에 절충점이 존재하며 본 실험에서 얻은 데이터가 연속장치의 설계와 운전에 중요한 기초자료가 될 것이다.

• Journal of Ecology and Environment
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• 제43권2호
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• pp.271-281
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• 2019

Background: The plant communities within reservoir drawdown zones are ecologically important as they provide a range of ecosystem services such as stabilizing the shoreline, improving water quality, enhancing biodiversity, and mitigating climate change. The aim of the study was therefore to identify the major environmental factors affecting these plant communities within the drawdown zone of the Soyangho Reservoir in South Korea, which experiences a monsoonal climate, and thereafter to (1) elucidate the plant species responses and (2) compare the soil seedbank composition along main environmental gradients. Results: Two main environmental gradients affecting the plant community structure were identified within the drawdown zone; these were a vertical and longitudinal gradient. On the vertical dimension, a hydrological gradient of flood/exposure, the annual-dominated plant community near the water edge changed to a perennial-dominated community at the highest elevation. On the longitudinal dimension from the dam to the upstream, plant species composition changed from an upland forest-edge community to a lowland riverine community, and this was correlated with slope degree, soil particle size, and soil moisture content. Simultaneously, the composition of the soil seedbank was separated along the vertical gradient of the drawdown zone, with mainly annuals near the water edge and some perennials at higher elevations. The species composition similarity between the seedbank and extant vegetation was greater in the annual communities at low elevation than in the perennial communities at higher elevation. Conclusions: The structures of plant community and soil seedbank in the drawdown zone of a monsoonal riverine reservoir were changed first along the vertical and secondly along the longitudinal gradients. The soil seedbank could play an important role on the vegetation regeneration after the disturbances of flood/exposure in the drawdown zone. These results indicate that it is important to understand the vertical and longitudinal environmental gradients affecting shoreline plant community structure and the role of soil seedbanks on the rapid vegetation regeneration for conserving and restoring the drawdown zone of a monsoonal reservoir.