• Journal of Microbiology and Biotechnology
• /
• v.27 no.4
• /
• pp.808-815
• /
• 2017

We demonstrated the quantitative detection of a toxin-producing Microcystis aeruginosa (M. aeruginosa) strain with the laboratory protocol of the NanoGene assay. The NanoGene assay was selected because its laboratory protocol is in the process of being transplanted into a portable system. The mcyD gene of M. aeruginosa was targeted and, as expected, its corresponding fluorescence signal was linearly proportional to the mcyD gene copy number. The sensitivity of the NanoGene assay for this purpose was validated using both dsDNA mcyD gene amplicons and genomic DNAs (gDNA). The limit of detection was determined to be 38 mcyD gene copies per reaction and 9 algal cells/ml water. The specificity of the assay was also demonstrated by the addition of gDNA extracted from environmental algae into the hybridization reaction. Detection of M. aeruginosa was performed in the environmental samples with environmentally relevant sensitivity (${\sim}10^5$ algal cells/ml) and specificity. As expected, M. aeruginosa were not detected in nonspecific environmental algal gDNA over the range of $2{\times}10^0$ to $2{\times}10^7$ algal cells/ml.

• Proceedings of the Korean Environmental Sciences Society Conference
• /
• 2000.05a
• /
• pp.251-254
• /
• 2000

In this study, we investigated vertical distribution and seasonal changes of phytophlankton community in the Hae-Dong Reservoir from March 1999 to Feburary 2000. This reservoir is relativly small (surface area, 2.7 $km^2$) and is the source of drinking water supply to the eastern part of Pusan City. Samples were collected at 2 sites (1, 3, and 6 m; site 1, in front of the dam; site 2, inlet). The dominant group was Bacillriophyceae at both sites (over 63%), and other groups exhibited seasonal changes (high cyanobacterial density in summer; green algal communities in winter). Chrysophyceae and Dinophyceae were maintained lower level during the study period. Along the water depth, all classess of phytoplankton did not show distintive vertical distribution at both sites except during the blue-green algal bloom in the middle of July and late September. The phytoplankton community dynamics in the Hoe-Dong Reservoir was strongly affected by the hydrological factors such as concentrated precipitation and short retention time.

• Journal of environmental and Sanitary engineering
• /
• v.19 no.2
• /
• pp.23-29
• /
• 2004

An algicidal effect of endemic algicidal bacterium (Pseudomonas putida) and rotifer zooplankton (Brachionus calyciflorus) on diatom Stephanodiscus hantzschii were examined in the filtered water, and were compared with those of bacterium plus ciliate. Bacteria removed as 80% of the diatom within 3.5 days, while ciliate and zooplankton suppressed 57% and 40% of diatom during the same period, respectively. Mixed treatment of bacteria plus ciliate removed as 54% of diatoms, while that of bacteria plus zooplankton decreased as 85%. Although single bacteria and mixed treatment of bacteria plus zooplankton quickly decreased the diatom in the initial of experiment, bacteria plus ciliate perfectly removed the diatom in culture flask within 5.5 days of the study. On the other hand, other single and mixed treatments did not clear the diatom during the same period, and over 10% of them still remain in culture flask. Predator biomass in the presence of algicidal bacteria showed the growth patterns; zooplankton gradually decreased, and ciliate sustained over 0.5 cells/ml. These results indicated that the addition of ciliate to the algicidal bacterium in controlling the diatom Stephanodiscus hantzschii is more effective than that of zooplankton. Therefore, this synergistic interaction relationship between the bacterium and ciliate play an important role in the bio-manipulation using bio-agents to control the diatomal bloom in freshwater lakes and streams.

• Journal of Korean Society of Water and Wastewater
• /
• v.23 no.4
• /
• pp.491-497
• /
• 2009

The purpose of this study is to propose a method of controlling redtide microbes which grow abundantly and form harmful algal bloom in eutrophic waterbody with yellow loess and hydrogen peroxide. In the laboratory test, hydrogen peroxide was applied to single species of C. polykrikoides and multispecies of redtide microbes. The seawater was evaluated by the pre-test analysis including chlorophyll-a, luminance and transmittance. The test results showed that both single and mixed species of redtide microbes could be controlled with the dose of 30mg $H_2O_2/L$. Residual hydrogen peroxide was completely decomposed with the addition of powdered yellow loess at 2g/L~10g/L. However, the decomposition rate of residual hydrogen peroxide for sintered granular yellow loess was relatively low compared to the use of powdered one. With the addition of dissolved oxygen concentration was increased at a rate of 0.013 mg DO/mg $H_2O_2$, which is a little lower than the one predicted theoretically. No evidence for any detrimental effects on Artemia, a type of brine shrimps, was shown up to the concentration of 100mg $H_2O_2/L$.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2010.05a
• /
• pp.1044-1048
• /
• 2010

해수를 이용한 세계최초의 해수수로인 송도 신도시 중앙공원 해수수로는 서해안에서 집수된 해수를 $5{\mu}m$의 공극 크기를 가지는 해수처리시설 (STF, seawater treatment facility)에 의해 여과된 뒤 2곳의 해수공급 지점에서 해수수로로 공급된다. 여과된 해수는 수로로 155L/sec의 유량으로 1.8km를 흐른 뒤 서해로 방류된다. 이러한 인공 해수수로에서는 흐름정체에 따른 수질 문제와 영양염에 의한 적조를 유발하는 조류 성장이 문제가 된다. 따라서 본 연구에서는 3차원 수치모형을 이용한 인공 해수수로내의 흐름특성 모의와 수질 모니터링의 통한 조류 생성 특성 및 장소를 비교하여 그 상관성을 분석하고자 한다. 수치모의 실험은 3차원 수치모형인 FLOW-3D를 이용하였으며, 격자망 형태는 직사각형 직각 격자가 사용되었다. 현장 모니터링을 통하여 획득한 수질 데이터와 비교 분석을 통하여 해수수로 내 해수 흐름 특성변화(유속, 와도 등)와 조류생성에 미치는 영향을 비교 분석하였다. 해수 수로내의 평균 유속은 0.01m/s 이었으며, 수로의 형상에 따라서 정체수역이 발생하였다. 이러한 정체수역은 현장 모니터링을 통하여 예측된 조류 발생 지역과 일치하였다. 이러한 흐름 특성과 조류생성과의 상관성 규명을 통하여 인공수로의 물순환 시스템 설계시 조류 생성을 억제할 수 있는 최적 관리를 위한 설계의 기초 자료를 제공할 수 있을 것으로 판단된다.

• Journal of the korean society of oceanography
• /
• v.39 no.4
• /
• pp.207-211
• /
• 2004

We used long time series of hydrographic and biological variables to examine the ecosystem consequences of a rare, anomalous event in the south sea of Korea. The highest zooplankton biomass in 36 years of sampling occurred in April 1997. Zooplankton biomass exceeded 2 times than the long-term mean at 35% of the stations. Copepod abundance was low in April and June and also failed to show a seasonal peak in 1997. Mackerel (Scomber japonicus) catches were very low in spring 1997 and 1999, in spite of a positive correlation between zooplankton biomass and mackerel catches at lags of 0, 12 and 24 months. It was discussed that a high zooplankton biomass with low copepod abundance in April 1997 resulted from unusual high temperature and salps abundance. Water temperatures were ca. $2^{\circ}C$ higher than the long-term mean at the surface. Salps and doliolids (thaliaceans), especially the warm-water species Doliolum nationalis, dominated the zooplankton. An unusual incursion of the Tsushima Warm Current may have transported the thaliaceans into the area and/or produced favorable conditions for a bloom. This study suggested that taxonomic composition of zooplankton was important to decide mackerel catches.

• Membrane and Water Treatment
• /
• v.10 no.6
• /
• pp.405-415
• /
• 2019

Microcystins (MCs) are toxins produced by cyanobacteria causing a major environmental threat to water resources worldwide. Although several MCs have been reported in previous studies, microcystin-LR (m-LR) has been extensively studied as it is highly toxic. Among the several techniques employed for the removal of this toxin, adsorption with AC has been extensively studied. AC has gained wide attention as an effective adsorbent of m-LR due to its ubiquity, high sorption capacity, cost effectiveness and renewability. In this review, the adsorption of m-LR onto AC was evaluated using the information available in existing scientific literature. The effects of the pore volume and surface chemistry of AC on the adsorption of m-LR considering the structural and chemical properties of ACs were also discussed. Furthermore, we identified the parameters that influence adsorption, including natural organic matter (NOM), pH, and ionic strength during the m-LR adsorption process. The effect of these parameters on MCs adsorption onto AC from previous studied is compiled and highlighted. This review may provide new insights into future activated carbon-m-LR adsorption research, and broaden its application prospects.

• Journal of Species Research
• /
• v.10 no.1
• /
• pp.46-56
• /
• 2021

Diatoms have been used in examining water quality and environmental change in freshwater systems. Here, we analyzed molecular profiling of seasonal diatoms in the Han River, Korea, using the hypervariable region of 18S V1-V3 rRNA and pyrosequencing. Physicochemical data, such as temperature, DO, pH, and nutrients showed the typical seasonal pattern in a temperate region. In addition, cell counts and chlorophyll-a, were recorded at high levels in spring compared to other seasons, due to the diatom bloom. Metagenomic analysis showed a seasonal variation in the phytoplankton community composition, with diatoms as the most frequently detected in spring (83.8%) and winter (69.7%). Overall, diatom genera such as Stephanodiscus, Navicula, Cyclotella, and Discostella were the most frequent in the samples. However, a large number of unknown Thalassiosirales diatoms were found in spring (35.5%) and winter (36.3%). Our molecular profiling revealed a high number of diatom taxa compared to morphological observation. This is the first study of diatoms in the Han River using molecular approaches, providing a valuable reference for future study on diatoms-basis environmental molecular monitoring and ecology.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2021.06a
• /
• pp.83-83
• /
• 2021

수리구조물로 인한 유황변화와 함께 기후변화로 기인하는 강우변동성 및 온도 증가는 수생태 전반에 악영향을 미치는 주요 인자로 작용하고 있다. 특히, 최근 가뭄으로 인한 유황감소 및 폭염 등으로 여름철 녹조의 발생 빈도 및 강도 증가가 지속적으로 증가하고 있다. 본 연구에서는 하천에서 계측되고 있는 Cyanobacteria 개체수를 기반으로 녹조발생 여부를 전망할 수 있는 모형을 개발하고자 한다. Cyanobacteria 개체수를 기준으로 녹조발생 여부를 판단할 수 있도록 기준값(threshold)을 설정하고 binary 형태로 시계열을 구성하였다. 이를 Bernoulli 모형에 적합하여 녹조 발생 여부를 판단할 수 있도록 모형을 개발하였다. 하천을 따라 나타나는 녹조는 시공간적으로 유사한 특성을 가지며, 이러한 점을 고려하여 여러 관측지점을 동시에 모델링하는 것이 모형의 효율성과 예측성 측면에서 유리하다. 본 연구에서는 낙동강을 따라 여러 녹조관측지점을 대상으로 동시에 모델링이 가능하도록 다변량 Bernoulli 모형 기반의 녹조 예측 모형을 제시하고 과거 자료를 대상으로 모형의 적합성을 평가하였다. 다양한 지표를 기준으로 교차검증을 수행하였으며, 기존 물리적 모델에 비해 모형의 예측성능 및 효율성 측면에서 우수성을 확인할 수 있었다.

• ALGAE
• /
• v.35 no.3
• /
• pp.225-236
• /
• 2020

Gymnodinium smaydae is a newly described mixotrophic dinoflagellate that feeds on only Heterocapsa spp. and Scrippsiella acuminata among 19 tested algal prey. It is one of the fastest growing dinoflagellates when feeding, but does not grow well without prey. To investigate its spatial-temporal distributions in Korean waters, we quantified its abundance in water samples that were seasonally collected from 28 stations along the Korean Peninsula from April 2015 to October 2018, using quantitative real-time polymerase chain reactions. This dinoflagellate had a wide distribution, as reflected by the detection of G. smaydae cells at 23 of the sampling stations. However, this distribution had a strong seasonality; it was detected at 21 stations in the summer and only one station in winter. The abundance of G. smaydae was significantly and positively correlated with chlorophyll a concentration as well as with water temperature. However, there were no significant correlations between the abundance of G. smaydae and salinity, concentrations of nutrients, or dissolved oxygen concentration. During the study period, G. smaydae was present when water temperatures were 7.6-28.0℃, salinities were 9.6-34.1, concentrations of NO₃ were not detectable-106.0 μM, and concentrations of PO₄ were not detectable-3.4 μM. The highest abundance of G. smaydae was 18.5 cells mL^-1 in the coastal waters of Jinhae in July 2017 when the chlorophyll a concentration was 127 mg m^-3 and water temperature was 23.8℃. Therefore, the spatial-temporal distribution of G. smaydae in Korean coastal waters may be affected by chlorophyll a concentration and water temperature.