• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2003.10a
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• pp.34-63
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• 2003

Occupational and environmental exposure to manganese continue to represent a realistic public health problem in both developed and developing countries. Increased utility of MMT as a replacement for lead in gasoline creates a new source of environmental exposure to manganese. It is, therefore, imperative that further attention be directed at molecular neurotoxicology of manganese. A Need for a more complete understanding of manganese functions both in health and disease, and for a better defined role of manganese in iron metabolism is well substantiated. The in-depth studies in this area should provide novel information on the potential public health risk associated with manganese exposure. It will also explore novel mechanism(s) of manganese-induced neurotoxicity from the angle of Mn-Fe interaction at both systemic and cellular levels. More importantly, the result of these studies will offer clues to the etiology of IPD and its associated abnormal iron and energy metabolism. To achieve these goals, however, a number of outstanding questions remain to be resolved. First, one must understand what species of manganese in the biological matrices plays critical role in the induction of neurotoxicity, Mn(II) or Mn(III)? In our own studies with aconitase, Cpx-I, and Cpx-II, manganese was added to the buffers as the divalent salt, i.e., $MnCl_2$. While it is quite reasonable to suggest that the effect on aconitase and/or Cpx-I activites was associated with the divalent species of manganese, the experimental design does not preclude the possibility that a manganese species of higher oxidation state, such as Mn(III), is required for the induction of these effects. The ionic radius of Mn(III) is 65 ppm, which is similar to the ionic size to Fe(III) (65 ppm at the high spin state) in aconitase (Nieboer and Fletcher, 1996; Sneed et al., 1953). Thus it is plausible that the higher oxidation state of manganese optimally fits into the geometric space of aconitase, serving as the active species in this enzymatic reaction. In the current literature, most of the studies on manganese toxicity have used Mn(II) as $MnCl_2$ rather than Mn(III). The obvious advantage of Mn(II) is its good water solubility, which allows effortless preparation in either in vivo or in vitro investigation, whereas almost all of the Mn(III) salt products on the comparison between two valent manganese species nearly infeasible. Thus a more intimate collaboration with physiochemists to develop a better way to study Mn(III) species in biological matrices is pressingly needed. Second, In spite of the special affinity of manganese for mitochondria and its similar chemical properties to iron, there is a sound reason to postulate that manganese may act as an iron surrogate in certain iron-requiring enzymes. It is, therefore, imperative to design the physiochemical studies to determine whether manganese can indeed exchange with iron in proteins, and to understand how manganese interacts with tertiary structure of proteins. The studies on binding properties (such as affinity constant, dissociation parameter, etc.) of manganese and iron to key enzymes associated with iron and energy regulation would add additional information to our knowledge of Mn-Fe neurotoxicity. Third, manganese exposure, either in vivo or in vitro, promotes cellular overload of iron. It is still unclear, however, how exactly manganese interacts with cellular iron regulatory processes and what is the mechanism underlying this cellular iron overload. As discussed above, the binding of IRP-I to TfR mRNA leads to the expression of TfR, thereby increasing cellular iron uptake. The sequence encoding TfR mRNA, in particular IRE fragments, has been well-documented in literature. It is therefore possible to use molecular technique to elaborate whether manganese cytotoxicity influences the mRNA expression of iron regulatory proteins and how manganese exposure alters the binding activity of IPRs to TfR mRNA. Finally, the current manganese investigation has largely focused on the issues ranging from disposition/toxicity study to the characterization of clinical symptoms. Much less has been done regarding the risk assessment of environmenta/occupational exposure. One of the unsolved, pressing puzzles is the lack of reliable biomarker(s) for manganese-induced neurologic lesions in long-term, low-level exposure situation. Lack of such a diagnostic means renders it impossible to assess the human health risk and long-term social impact associated with potentially elevated manganese in environment. The biochemical interaction between manganese and iron, particularly the ensuing subtle changes of certain relevant proteins, provides the opportunity to identify and develop such a specific biomarker for manganese-induced neuronal damage. By learning the molecular mechanism of cytotoxicity, one will be able to find a better way for prediction and treatment of manganese-initiated neurodegenerative diseases.

• Journal of Aquaculture
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• v.9 no.3
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• pp.223-232
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• 1996

Growth and survival rate in shortnecked clam, Ruditapes philippinarum were studied from different seedling production areas, Hadong, Ulsan and Kochang to Kwangyang Bay, Korea, and three stocking densities (1,000, 1,500 and 3,000 individuals/$m^2$) of clam from Ulsan seedling production area were examined. During the experimental period, water temperature and specific gravity were ranged from 4.5 to $26.0^{\circ}C$ and from 1.0216 to 1.0248, respectively. Shell length was grown from $2.30{\pm}0.05\;to\;3,29{\pm}0.02$ cm in Hadong, $1.32{\pm}0.02\;to\;3.15{\pm}2.02$ cm in Ulsan, $1.24{\pm}0.03\;to\;3.18{\pm}0.02$ cm in Kochang. Kochang's seed was displayed fast growth rate than others. In different rearing densities, the lower density group was showed a rapid growth rate. Shell length from the 1,000 individuals per $m^2$ group grew longer than that from the 2,000 individuals group. Total weight from the 1,000 individuals group was higher than that from the 1,5()0 individual and the 2,000 individual groups. Survival rate by seedling production areas, Hadong, Ulsan and Kochang showed $69.4\%,\;63.8\%\;and\;41.7\%$, respectively. Survival rate by rearing density, 1,000, 1,500 and 2,000 individual groups showed $70.8\%,\;67.8\%\;and\;59.0\%$, respectively.

• Economic and Environmental Geology
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• v.45 no.5
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• pp.513-523
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• 2012

The upper sedimentary layer of the Ulleung Basin in the East Sea shows stacked mass-flow deposits such as slide/slump deposits in the upper slope, debris-flow deposits in the middle and lower slope, and turbidites in the basin plain. Shallow gases or gas hydrates are also reported in many area of the Ulleung Basin, which are very important in terms of marine resources, environmental changes, and geohazard. This paper aims at studying acoustic characteristics and distribution pattern of gas-related structures such as acoustic column, enhanced reflector, dome structure, pockmark, and gas seepage in the upper sedimentary layer, by analysing high-resolution chirp profiles. Acoustic column shows a transparent pillar shape in the sedimentary layer and mainly occurs in the basin plain. Enhanced reflector is characterized by an increased amplitude and laterally extended to several tens up kilometers. Dome structure is characterized by an upward convex feature at the seabed, and mainly occurs in the lower slope. The pockmark shows a small crater-like feature and usually occurs in the middle and lower slope. Gas seepage is commonly found in the middle slope of the southern Ulleung Basin. These gas-related structures seem to be mainly caused by gas migration and escape in the sedimentary layer. The distribution pattern of the gas-related structures indicates that formation of these structures in the Ulleung Basin is controlled not only by sedimentary facies in upper sedimentary layer but also by gas-solubility changes depending on water depth. Especially, it is interpreted that the chaotic and discontinuous sedimentary structures of debris-flow deposits cause the facilitation of gas migration, whereas the continuous sedimentary layers of turbidites restrict the vertical migration of gases.

• Economic and Environmental Geology
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• v.51 no.5
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• pp.401-407
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• 2018

For the treatment of heavy metals in the mine drainage from the closed mine area, various methods such as passive, active and semi-active treatments are considered. Among contaminated elements in the mine drainage, Mn is one of the difficult elements for the treatment because it needs high pH over 9.0 for its concentration to be reduced. In this study, the efficiency of various slag complex reactors (slag (S), slag+limestone (SL) and slag+Mn coated gravel (SG)) on Mn removal in the presence of Fe, which is a competitive element with Mn, was evaluated to investigate effective methods for the treatment of Mn in mine drainage. As a result of experiments on Mn removal without Fe during 358 days, using influent with $30{\sim}50Mn{\cdot}mg/L$ and pH 6.7 on the average, S reactor showed continuously high Mn removal efficiency with the average of 99.9% with pH 8.9~11.4. Using the same reactors, Mn removal experiments with Fe during 237 days were conducted with the influent with $40{\sim}60Mn{\cdot}mg/L$. The pH range of effluent reached to 6.1~10.0, which is slightly lower than that of effluent without Fe. S reactor showed the highest range of pH with 7.1~9.9, followed by S+L and S+G reactor. However, the efficiency of Mn removal showed S+L>S>S+G with the range of 94~100%, 68~100% and 68~100%, respectively in spite of relatively low pH range. S+L reactor showed the most resistance on Fe input, which means other mechanisms such as $MnCO_3$ formation by the carbonate prouced from the limestone or autocatalysis reaction of Mn contributed to Mn removal rather than pH related mechanisms. The evidence of reactions between carbonates and Mn, rhodochrosite ($MnCO_3$), was found from the X-ray diffraction analysis of precipitates sample from S+L reactor. From this study, the most effective reactors on Mn removal in the presence of Fe was S+L reactor. The results are expected to be applied for the Mn containing mine water treatment in the presence of Fe within the relatively low range of pH.

• Horticultural Science & Technology
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• v.29 no.4
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• pp.311-316
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• 2011

This study aimed to determine the effect of EC (electrical conductivity) levels of nutrient solution in hydroponic culture on allyl-isothiocyanate (AITC) content within plant tissues, Vitamin C content and physiological responses in wasabi plant (Wasabia japonica M. 'Darma'). The 'Darma' was grown for 5 weeks with a deep flow technique (DFT) system controlled at 5 different EC levels, including 0.5, 1, 2, 3, and $5dS{\cdot}m^{-1}$. In result, the highest total content of AITC showed at EC level 5 and $3dS{\cdot}m^{-1}$ for 1 or 5- week, respectively. The total content of AITC increased about 1.2-1.4 times when the plants were grown in the EC levels between 0.5 and $2dS{\cdot}m^{-1}$, whereas the content decreased about 6 and 56 % in the EC level 3 and $5dS{\cdot}m^{-1}$, respectively. The content of AITC was relatively higher in petiole tissue, about 53 %, taken from 1 week-grown plants when the EC was controlled between 0.5 and $2dS{\cdot}m^{-1}$. Root tissue also had relatively higher content of AITC, about 45.1 %, when the EC was controlled at 3 and $5dS{\cdot}m^{-1}$. However, a 5-fold decrease in the AITC content was found in blade tissue and a 6.8-fold decrease in root when the EC was controlled at $5dS{\cdot}m^{-1}$ for 5 weeks. There was no significant difference in the vitamin C content in 1-week grown leaf tissues under the different EC level treatments; but, the content increased about 27% in 5-week grown plants at the EC level between 0.5 and $2dS{\cdot}m^{-1}$, compared to the 1 week-grown leaf tissue. Electrolyte leakage of leaf tissue taken from 3-week grown plant was 3-fold higher at the EC level $5dS{\cdot}m^{-1}$, compared to the EC level between 0.5 and $2dS{\cdot}m^{-1}$. Chlorophyll content, photosynthesis rate and transpiration rate were decreased when the EC was controlled at higher than $2dS{\cdot}m^{-1}$. Leaf water content, specific leaf area and growth were decreased when the EC was controlled at $5dS{\cdot}m^{-1}$ for 5 weeks. All the integrated results in this study suggest that the EC level of nutrient solution should be maintained at lower than $3dS{\cdot}m^{-1}$ in order to improve nutritional value and quantity required for hydroponically grown wasabi as functional vegetable.

• Korean Journal of Ecology and Environment
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• v.45 no.4
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• pp.482-489
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• 2012

Visual monitoring is hard to apply on fish because they are living in a water system. To overcome this problem, acoustic telemetry, which is effective for underwater monitoring, is often used for studying fish behaviors, such as movement distance, route and patterns. In this study, in order to monitor the movement pattern of Squaliobarbus curriculus (family Cyprinidae), we used acoustic telemetry and identified the home range and movement distances. A total of nine individuals were released at two different locations: one is at the estuary barrage (Sc1~3) and the other is at the lower part of Baekjae Weir (Sc4~9), located in Geum River. Approximately, a 70 km section from the estuary barrage was investigated. Fish, which were released at the estuary barrage, utilized up to 12.7 km upstream as home range from the release site. At the lower part of Baekjae Weir, most of the fish moved and stayed within a 7.2 km downstream area, except for Sc6, which moved 53.4 km (linear maximum distance from release site) downstream from the release site. Relatively small sized fish (Sc7~9) did not show any movement. Accumulated movement distance significantly correlated with the standard length of S. curriculus ($r_s$=0.715, p=0.03). Moreover, the standard length of moving fish was significantly larger than that of not moving fish (Mann-Whitney U test, p=0.024). Therefore, the movement distance of S. curriculus has been correlated with fish size; movement distance was increased with the standard fish length. Although the sample size of monitored fish was small, various meaningful data were collected by acoustic telemetry. Consequently, this technique could be a method available for effectively monitoring the behavior and ecology of native Korean and endemic species.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.2
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• pp.155-163
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• 1999

Nutrient balance during rice cultivation in the paddy of a local area under the environmental protection for drinking water supply was investigated. To compare nutrient balance in the paddy soil applied with different types of fertilization, 7 treatments were selected as followings : Recommended level of chemical fertilizers(R), Conventional fertilization(CF), Fresh cow manure(FCM), Cow manure compost(CMC), Straw compost+reduced chemical fertilizer(SCF), Fresh straw+recommended level of fertilizers(FSC), and no fertilization as control(C). Here, FCM, CMC and SCF were applied at the same level of total nitrogen as recommended in R. Rice yield was the highest in the recommendation(R) and fresh cow manure (FCM) treatments with $6,730kg\;ha^{-1}$(index 100), and followed by SCF (index 98), FSC (index 98), CMC(index 94), and CF(index 94). But statistically significant difference was not recognized among treatments except the control. Nitrogen infiltration loss was high in the simple chemical fertilizer treatments with $63kg\;ha^{-1}$ in CF and $58kg\;ha^{-1}$ in R during rice cultivation, respectively. Nitrogen infiltration loss was decreased below half level of chemical fertilizer treatments with cow manure treatments ($23kg\;ha^{-1}$ in FCM and $27kg\;ha^{-1}$ in CMC) and with reducing chemical fertilizer treatment by adding straw compost ($25kg\;ha^{-1}$). Phosphate was not leached during rice cultivation in paddy soil of a fluvial deposit type, in which oxidation horizon was developed broadly under around 15 cm depth of surface soil. Phosphate balance (A-B) was closed to 0 in all treatments except cow manure treatment (CMC), in which it was $+30kg\;ha^{-1}$ and show the possibility of over accumulation of phosphate by continuously replicated application of cow manure compost. Potassium balance was negative value in all but straw recycling treatment (FSC). It means that potassium was continuously supplied from soil minerals, uptaken by plants or eluted out of soil. In conclusion, by substituting inorganic fertilizer for organic fertilizer or reducing application rate of chemical fertilizer through mixing organic fertilizer, it would be possible to achieve the same rice yield as in the recommendation treatment and to decrease nutrient leaching below half level in rice paddy soil.

• Korean Journal of Organic Agriculture
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• v.26 no.3
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• pp.439-452
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• 2018

This study was carried out to establish of environment-friendly control system and to survey the occurrence status of Ricania shanthungensis in Jeonnam province from 2016 to 2017. R. shanthungensis occurred at 1,344 ha in 61 towns of 15 cities in Gwangju and Jeonnam province. At four sites in the stationary area, the densities of eggmass and pupae were lower in 2017 than in 2016, the hatching rate decreased, and the first hatching time, emergence of adults, and the start of spawning tended to be slower. Telenomus sp., an egg parasitoid of R. shanthungensis, was found in most areas. Sunchon had the highest rate of 46.1%, followed by Muan 40.6, Goksung 29.2, Gurye 25.8, and Gwangju 17, respectively. The optimum spraying time for the control of R. shanthungensis was early March. The spraying material and the dilution multiples are 10 times of the machine oil at environmental friendly cultivation and 500 times of the chlorpriphosphate wp. at normal cultivation. When sprayed these materials, 95% of egg prohibited to hatch. There was more than 80% of the insecticidal effects on the organic materials of the machine oil emulsion, the Sophora root extrect + microbial extract agent, and the castor emulsion. For adults, Sophora root extrect + microbial extract, neem extract, and machine oil were better. Four kinds of chemical pesticides such as dinotefuran wp were effective for nymph and adult control. We have developed an adult catching device using the most preferred daylight color and behavioral habits of R. shanthungensis. The capture device consisted of two daylight compact lamps (30W and 20W), a yellow plate, and a catcher using water, and caught about 700 individuals a day. Based on the above results, we have established a system for controlling and life cycle of R. shanthungensis in Jeonnam province.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.16 no.2
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• pp.81-96
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• 2011

Sediment oxygen demand(SOD) and denitrification rates were measured in four major estuaries(Suncheon Bay, Seomjin river estuary, Goseong stream estuary and Masan Bay) in south coast of Korean peninsula from March of 2009 to May 2010 to estimate organic matter cleaning capacity. SOD was estimated from the temporal dissolved oxygen concentration change and isotopic pairing technique was employed to measure denitrification. Sediment oxygen demand(SOD) was ranged from -5.1 to 24.6 mmole $O_2m^{-2}d^{-1}$ and denitrification rate was ranged from 0.0 to 3.9 mmole $N_2m^{-2}d^{-1}$in the study area. SOD was the highest in Masan Bay(-2.2 to 19.2, average = 10.2 mmole $O_2m^{-2}d^{-1}$) and Suncheon, Goseong, Tae-an and Seomjin followed. Denitrification was also the highest in Masn Bay(0.0 to 3.9, average = 1.0 mmole $N_2m^{-2}d^{-1}$) and Goseong, Seomjin, Suncheon and Taean followed. The effect of benthic photosynthesis by microphytobenthos on denitrification was evident in some season of Tae-an, Seomjin, and Masn Bay. The increased oxygen level produced by photosynthesis stimulated nitrification without severe adverse effect on denitrification and, as a result, coupled nitrification and denitrification was enhanced in these areas. A difference of seasonal patterns of denitrification at each site depended on relative importance of denitrification on different nitrate source($D_w$: nitrate from water column and $D_n$: nitrated produced during nitrification). Denitrification was maximum during spring in Goseong, Suncheon and Masan Bay. On the contrary, denitrification was the highest during summer in Tae-an and Seomjin estuary.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.20 no.2
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• pp.78-91
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• 2015

Macrobenthic community was studied at 87 stations including intertidal and subtidal area in Yoja Bay, south coast of Korea in summer season of July 2001. Duplicate sediment samples were taken using a van Veen grab ($0.1m^2$) in each station. Mud facies of the sediments were widly distributed in the bay. And relatively high content of sand was shown in the Bulgyo-cheon stream estuary. A total of 274 species was occurred with a mean density of $2,346ind./m^2$ and a mean biomass of $78.2g/m^2$. The polychaetes were species- and density-dominant faunal group with a total of 122 species (44.5% of the total number of species), and mean density of $1,543ind./m^2$ (65.8% of the mean density). Meanwhile, molluscs were biomass-dominant faunal group with $44.4g/m^2$. Bio-Env. analysis showed that the combination of bottom salinity and sand content of the surface sediments was highly correlated to the major macrobenthic communities. The macrobenthic species number, decreasing toward inner bay from mouth of the bay, was significantly correlated to the sediment environmental variables and bottom water salinity. The spatial distribution of abundance showed significant correlation to the sand and mud contents and mean grain size of the surface sediments. Major dominant species were Minuspio japonica (polychaete) with a mean density of $1,167ind./m^2$ at upper part of the bay where salinity was low and Eriopisella sechellensis (amphipod) with $152ind./m^2$ in central part of the bay. Species diversity (H') was $3.0{\leq}$ in the mouth part of the bay and ranged 2.0-3.0 in the inner part of the bay, which showed a significant positive correlation to bottom salinity. Total number of species also showed significant correlations to the sediment composition and bottom salinity. Based on the cluster analysis the macrobenthic community of the bay was classified into five station groups from the bay mouth toward the inner part of the bay depending on the species composition. From the SIMPER analysis Minuspio japonica, Eriopisella sechellensis and Sternaspis scutata mainly contributed to the classification of station group. These results suggested that the macrobenthic communities of the bay were mainly influenced by bottom salinity together with sediment composition, and that the studies of spatial distributions of major dominant species and benthic communities should be conducted continuously to monitor the Yeoja Bay benthic environment.