• 생태와환경
• /
• 제36권2호통권103호
• /
• pp.208-214
• /
• 2003

국내산 물벼룩의 배양조건에 따른 개체 생산특성을 비교하고 최적 배양환경 조건을 도출하기 위하여 배양조건에 따른 물벼룩의 개체생산특성을 조사하였다. Daphnia magna, D. pulex, Moina macrocopa는 퇴비를 이용한 배양액으로 배양하는 것이 생존기간과 개체 발생 수 측면에서 가장 효과가 좋았으며 D. galeata는 이와는 달리 비료를 이용한 배양액에서 가장 성장률이 좋았다. D. galeata는 전반적으로 배양이 어려웠으며 배양기간 동안의 새끼 발생도 적어 인공 배양시에 상당한 관심이 요구되었다. 남조류 Microcystis sp.의 microcystin이라는 독소는 포유류를 비롯한 여러 생물에게 직접적인 영향을 미치는 것으로 밝혀졌으나 금번 실험에서는 물벼룩의 생존여부에 대해서는 독소의 영항이 크게 나타나지 않았다. 또한 남조류 Microcystis sp.를 공급받은 물벼룩은 제한된 먹이 제공으로 적은 양의 Microcystis sp.를 불가피하게 섭취함으로써 상대적으로 생존기간이 짧았으며 개체 생산율도 낮았다. Scenedesmus sp.를 먹이로 공급한 경우는 물벼룩의 종류와 배양액의 종류에 상관없이 Microcystis sp.를 먹이로 공급한 경우보다 생존기간이 길고 개체발생수도 많았다. 각 물벼룩은 $20^{\circ}C$에서보다 더 생존기간이 길고 더 많은 개체를 발생하여 물벼룩 배양은 $15^{\circ}C.$보다 $20^{\circ}C$가 적절함이 확인되었다. 이러한 결과를 토대로 볼 때 D. galeata와 M. macrocopa는 물벼룩을 이용한 수질감시와 독성실험에 사용하기에 어려움이 많은 종이며 기존에 사용하고 있는 D. magna와 국내에서도 분포하고 있는 D. pulex를 사용하는 것이 용이할 것으로 사료된다.

• 한국균학회지
• /
• 제35권1호
• /
• pp.37-42
• /
• 2007

잣버섯 균사체 배양에 적합한 생물반응기는 풍선형의 공기부양식의 생물반응기가, 수용성 다당체의 생산에도 생장이 좋았던 풍선형 공기부양식 생물반응기가 유리한 것으로 나타났다. 배지의 탄소원 농도별에 따른 수용성 세포외 다당체의 생산량은 농도 처리간 차이가 없었으나 수용성 세포내 다당체는 탄소원 농도가 낮은 즉 C/N율이 낮을수록 증가하였다. 잣버섯 균사체는 고농도의 배지 배양조건에서 생물반응기내로 배지 공급형태가 배지량 증가방법보다 배지농도 조절에 의한 연속 배양방법이 우수한 것으로 나타났다.

• Mycobiology
• /
• 제35권4호
• /
• pp.210-214
• /
• 2007

Antibacterial and antifungal activities of liquid culture filtrate, water and ethanol extract (solid culture) of Stereum ostrea were evaluated against 5 bacteria and 3 plant pathogenic fungi. To determine the minimal inhibitory concentration (MIC), we studied $5{\sim}300\;mg/ml$ concentrations against bacteria and fungi separately. The MIC was 10 mg/ml for Bacillus subtilis and 40 mg/ml for Colletotrichum gloeosporioides and Colletotrichum miyabeanus. Liquid culture filtrate was more effective against Gram positive than Gram negative bacteria, and Staphylococcus aureus was the most inhibited (20.3 mm) bacterium. Water and ethanol extracts were effective against both Gram positive and Gram negative bacteria, and water extract was better than ethanol extract. In water and ethanol extract, inhibition zones were 23.6 and 21.0 mm (S. aureus) and 26.3 and 22.3 mm (Pseudomonas aeruginosa), respectively. For plant pathogenic fungi, the highest and lowest percent inhibition of mycelial growth (PIMG) was found 82.8 and 14.4 against C. miyabeanus and Botrytis cinerea in liquid culture filtrate, respectively. In water extract, the PIMG was found to be the highest 85.2 and lowest 41.7 for C. miyabeanus and C. gloeosporioides, respectively. The inhibitory effect of ethanol extract was better against C. miyabeanus than C. gloeosporioides and B. cinerea. Among 3 samples, water extract was the best against tested pathogenic fungi. This study offers that the extracts isolated from S. ostrea contain potential compounds which inhibit the growth of both bacteria and fungi.

• The Korean Journal of Physiology
• /
• 제3권1호
• /
• pp.45-49
• /
• 1969

The effects of water extracts and powder of Korean ginseng on the division of Saccharomyces cerevisiae were studied. 1. The addition of several doses of water extracts and powder of ginseng to the yeast medium of Moyer and Coghill showed various promoted division of Saccharomyces. 2. The optimal dose of ginseng on tile division of Saccharomyces (0.08% dry ginseng medium solution per $10\;cells/mm^3$) could be recognized. 3. On the culture for 24 hours at $18^{\circ}C$, the cell number of control group was $13.25{\times}10^3\;cells/mm^3$ and that of the optimal dose group of water extracts of ginseng was $23.20{\times}10^3\;cells/mm^3$. On the culture, for 24 hours at $25^{\circ}C$, the cell number of control group was $16.85{\times}10^3\;cells/mm^3$ and that of the optimal dose group was $30.20{\times}10^3\;cells/mm^3$. The increasing rate of cell divison by the ginseng was about twice than that of control group. The optimal dose treatment of ginseng at $18^{\circ}C$ was more effective than control group at $25^{\circ}C$. 4. On the culture for 24 hours at $18^{\circ}C$, the increasing rate of water extracts of ginseng was 75.1%, and the rate of ginseng powder was 7.6%. On the culture for 24 hours at $25^{\circ}C$, the rate of water extracts of ginseng was 79.8%, and the rate of ginseng powder was 57.2%. Therefore water extracts of ginseng was more effective than ginseng powder of same dry weight, and the promoted effect of ginseng powder at $25^{\circ}C$ was more effective than at $18^{\circ}C$.

• 한국잡초학회지
• /
• 제8권1호
• /
• pp.59-63
• /
• 1988

수도(水稻) 담수직파재배(湛水直播栽培)에서 제초제(除草劑) 처리(處理)에 따라 야기되기 쉬운 약해(藥害)가 안전성(安全性)이 있는 제초제(除草劑)를 선발하기 위하여 이앙답(移秧畓)에서 사용되고 있는 4종(種)의 제초제(除草劑)를 공시(供試)하여 간척답(干拓畓)과 보통답(普通畓)의 토양(土壤)을 사용하여 폿트실험으로 벼의 안전성(安全性)을 조사하였다. 1. Pyrazolate는 300, 600 g ai/10a에서 벼에 안전하여 입모율(立毛率)이 높고 벼의 초장(草長), 경수(莖數) 및 건물중(乾物重)의 감소가 적었다. 2. Bensulfuron methyl 5.1g ai/10a을 파종직후(播種直後)에 처리(處理)하면 약해(藥害)가 유발되었으나 파종(播種) 5 일 후(後)에 처리(處理)하므로써 약해(藥害)가 현저히 감소되었으며, 10.2g ai/10a에서는 다소 약해(藥害)가 크게 나타났다. 3. Chlormethoxynil은 210g ai/10a에서도 약해(藥害)가 나타나 입모율(立毛率)이 낮아지고 벼의 초장(草長), 경수(莖數) 및 건물중(乾物重)도 크게 감소되었다. 4. Benthiocarb는 210g ai/10a을 파종(播種) 5 일(日) 후(後)에 처리(處理)하면 약해(藥害)가 적었으나, 파종직후처리(播種直後處理)와 420g ai/10a 처리(處理)에서는 약해(藥害)가 많은 경향이었다.

• 한국환경농학회지
• /
• 제19권5호
• /
• pp.433-441
• /
• 2000

A feasibility study was performed to examine the agronomic application of treated sewage on paddy rice culture by field experiment for two consecutive years. The domestic sewage was treated by the constructed wetland system which was in subsurface flow type and consisted of sand and macrophyte. The effluent of the wetland system was used for irrigation water. The effluent was diluted to maintain the total nitrogen concentration below $26mg{\cdot}L^{-1}$ in the first year and used without dilution in the second year experiment. Growth components and yields were compared against the CONTROL plot where conventional method was applied. And also, soil characteristics of the plots before and after reclaimed sewage irrigation were analyzed. Generally, addition of the treated sewage to the irrigation water showed no adverse effects on paddy rice culture, and even enhancement was noticed in both growth and yield. Irrigation of treated sewage after concentration adjusted with conventional fertilization showed the better result, and the yield exceeded that of CONTROL case where clean water was irrigated. Soil characteristics changed after irrigation, and significant EC increasing was observed for the reclaimed sewage irrigation plots. From this study, it appears that reuse of treated sewage, as supplemental irrigation water could be a feasible and practical alternative. For full-scale application, further study is recommended on the specific guideline of major water quality components in treated sewage for irrigation and public health.

• 한국산학기술학회논문지
• /
• 제5권1호
• /
• pp.7-12
• /
• 2004

급액관리에 관련된 단계를 4단계로 나누었으며, 각 단계별 수분 이동 저항을 규명하고, 단계 중에서 급액 상황에 따라 관련 인자의 priority를 설정함으로써 펄라이트 자루재배할 경우 율속인자 중심의 적정 급액관리 기준을 제안하였다. 특히, 급액관리의 가장 중요한 율속인자인 뿌리까지의 수분도달거리를 최소한으로 하여 필요한 수분을 공급하는 과정과 역사성을 부여한 뿌리발달 조장이 가장 중요한 것으로 판명되었다.

• 유기물자원화
• /
• 제7권1호
• /
• pp.1-12
• /
• 1999

인공 배양토의 기능을 향상시키기 위해서 정수장 슬러지를 주원료로한 인공 배양토 개발에 관한 연구를 수행하였다. 기존에 시판되고 있는 인공 배양토보다 제지 슬러지, 하수 슬러지, 완숙 퇴비, 정수장 슬러지를 각각 40, 20, 10, 30% 혼합하여 제조한 인공 배양토가 perennial ryegrass, 싸리나무에 대한 식물생육에 효과적이었다. 식물독성실험에서도 정수장 슬러지를 혼합한 시료에서는 식물독성이 나타나지 않았다. 또한 정수장 슬러지는 인공 배양토의 보수력 및 접착력 증가에 긍정적인 영향이 있었다. 정수장 슬러지는 알루미늄을 다량 함유하고 있으나, 퇴비와 혼합하였을 경우 pH 2의 낮은 pH에서 Al의 용출이 거의 없었다.

• 한국식품영양과학회지
• /
• 제28권1호
• /
• pp.233-239
• /
• 1999

This study was performed to evaluate the effects of the supplementation of lactic acid bacterias (LAB; S. thermophilus, L. acidophilus) aluminum accumulation and function kidney in rats treated with 250 g/g aluminum sulfate for 4 weeks. Fifty male Sprague Dawley strains were divided into five groups according to the types of supplement. the control, S. thermophilus culture group(A), autoclaved S. thermophilus culture(B), L. acidophilus culture group(C) and autoclaved L. acidophilus(D). The weight gain was increased by supplementation of S. thermophilus culture(A), autoclaved S. thermophilus culture(B), L. acidophilus culture(C), autoclaved L. acidophilus culture(D), especially by supplementation of S. thermophilus cultured(A) as compared to control group. The amount of water intake was increased in control group as compared to the LAB supplemented group. The content of aluminum were decreased 19.57%, 31.25%, 37.10%, and 32.40% in kidney after supplementation of non autoclaved culture group (A,C), and autoclaved group(B,D) respectively, as compared to control group. Water balance, urine volume, and excretion of sodium and potassium decreased in LAB supplemented group and control group. Excretion of creatinine increased in control group and tendency to increased in LAB supplemented group. In conclusion, the effect of suppression of aluminum accumulation was more effective in sup plementation of S. thermophilus culture(A), and L. acidophilus culture(C) than autoclaved S. thermo philus culture(B), and L. acidophilus culture(D).

• 한국환경보건학회지
• /
• 제14권1호
• /
• pp.23-32
• /
• 1988

A study was carried out to find distribution of Legionella spp. in hot water supplying systems of buildings in Seoul. Water samples were taken from the hot water taps in three research institute buildings during the period from the 2nd to 27th February, 1987. And all of the three buildings were supplied with hot water from the same central hot water tank. GVPC BCYEa and blood agar were used for the isolation of Legionella pneurnophila and slide agglutination test (SAT) was performed to identify their strain types. Main findings were as follows 1. 44 samples taken from hot tap-water revealed 20% of positive culture and 44 samples taken from hot water spouts revealed 11% of positive culture. 2. The 78% of positive samples taken from hot water was obtained from 25.1$\circ$C~45.0$\circ$C temperature range. 3. Only, Legionella pneumophila serogroup 3 was identified and most of them has been than 2$\mu$m in length. 4. The densities of the Legionella pneurnophila isolated from hot water samples were within the range of $1.0\times 10^2$ to $1.1\times 10^3$ CFU/l and their densities isolated from hot water spouts were either 1 CFU/plate or 2 CFU/plate.