• Journal of Korean Society of Environmental Engineers
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• v.27 no.5
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• pp.555-561
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• 2005

A new graphical method was developed to separate two distinctive decay rate coefficients($k_1$ and $k_2$) at their respective degradable substrate fractions($S_1 and $S_2$). The mesophilic batch reactor showed $k_1$ of $0.151\;day^{-1}$ for wasted activated sludge(WAS), $0.123\;day^{-1}$ for thickened sludge(T-S), $0.248{\sim}0.358\;day^{-1}$ at S/I ratio of $1{\sim}3$ for sorghum and $0.155{\sim}0.209\;day^{-1}$ at S/I ratio $0.2{\sim}1.0$ for swine waste, whereas their long term batch decay rate coefficients($k_2$) were $0.021\;day^{-1}$, $0.001\;day^{-1}$, $0.03\;day^{-1}$ and $0.04\;day^{-1}$ respectively. At least an order of magnitude difference between $k_1$ and $k_2$ was routinely observed in the batch tests. The portion of $S_1$, which degrades with each $k_1$ appeared 71% for WAS, 39% for T-S, 90% for sorghum, and $84{\sim}91%$ at S/I ratio of $0.2{\sim}1.0$ for swine waste. Ultimate biodegradabilities of 50% for WAS, 40% of T-S, $82{\sim}92%$ for sorghum, and $81{\sim}89%$ for swine waste were observed.

• Korean Journal of Environmental Agriculture
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• v.23 no.1
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• pp.22-27
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• 2004

The activated paper mill sludge was treated with WF and some additives (sodium sulfide, nickel nitrate, ethyl acetate) for methane fermentation at $35^{\circ}C$. Optmum C/N ratio was 60 out of three conditions (20, 30 and 60). The Period of 40% of methane content possibly ignition, was 2 days shorter than with non-treatment during 10 days. Nevertheless, the total amount of methane production showed the 1/8 level of control far the same period. The yield and content of methane were increased by the addition of sodium Sulfur and ethyl acetate. Sulfur was an essential factor in methane fermentation of paper mill sludge.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.8 no.3
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• pp.113-118
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• 1988

Laboratory aerobic and anaerobic digesters were operated to determine the nonbiodegradable fractions in PS (Primary Sludge) and WAS (Waste Activated Sludge)from a municipal wastewater treatment plant in Seoul. Nonbiodegradable fractions were determined to be 45 to 47% in PS and 58 to 68% in WAS. Ultimate $CH_4$ gas production rates were estimated to be 0.76 for PS and $0.54m^3/kg$ VS removed for WAS. $CH_4$ contents were respectively 63% for PS and 65% for WAS.

• Journal of Korean Society of Environmental Engineers
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• v.37 no.1
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• pp.60-68
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• 2015

The study of waste activated sludge (WAS) solubilization has been increased for sludge volume reduction and enhancing the efficiency of anaerobic digestion. Microwave (MW)-assisted solubilization is an effective method for the solubilization of WAS because this method can lead to thermal, nonthermal effect and ionic conduction by dielectric heating. In this study, the solubilization of WAS by MW heating and conductive heating (CH) was compared and to enhance the MW-assisted solubilization of WAS at low MW output power, chemical agents were applied such as $H_2SO_4$ as the strong acid and $CaCl_2$, NaCl as the ionic materials. Compared to the COD solubilization of WAS by CH, that by MW heating was approximately 1.4, 6.2 times higher at $50^{\circ}C$, $100^{\circ}C$, respectively and the highest COD solubilization of WAS was 10.0% in this study of low MW output power condition. At the same MW output power and reaction time in chemically agents assisted experiments, the COD solubilization of WAS were increased up to 18.1% and 12.7% with the addition of $H_2SO_4$ and NaCl, however, that with the addition of $CaCl_2$ was 10.7%. This result might be due to the fact that the precipitation reaction occurred by calcium ion ($Ca^{2+}$) and phosphate ion (${PO_4}^{3-}$) produced in WAS after MW-assisted solubilization. In this study, $H_2SO_4$ turned out to be the optimal agent for the enhancement of MW efficiency, the addition of 0.2 M $H_2SO_4$ was the most effective condition for MW-assisted WAS solubilization.

• Korean Journal of Soil Science and Fertilizer
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• v.31 no.1
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• pp.39-45
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• 1998

As a basic research for developing a sludge application technique in forestry, this study was conducted to investigage a reasonable amount and period of sludge to be applied onto forest nursery of Acer palmatum Thunb. The paper mill sludge was used for the study, and ammonium nitrate was treated to some of them to reduce the C/N ratio lower than 40. The maximum amount of applied sludge was 7ton/ha/year, and they were spread on spring and on late summer. The sludge application did not stimulate early growth of the trees during a year. Since there was no significant changes in soil chemical properties, the amount of applied sludge was thought to be too little to result in a significant role for tree growth. However, the soil water showed significant increase of Cu when the activated sludges were applied during late summer. Thus, the time of application need to be determined cautiously with considering the risk of ground water contamination. In conclusion, if we set a goal from the view point of long-term concept such as forest productivity rather than to get a short-term product, the uncomposted sludge in small amount during growing season might be considered to be applied on forest nursery.

• Asian-Australasian Journal of Animal Sciences
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• v.6 no.2
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• pp.205-209
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• 1993

A new treatment system for animal waste water has been developed as an alternative to the activated sludge process. It consists of two treatments; one is operated with 7 tanks, and the other is soil and plant cultivation bed. Aerobic microorganisms are added to the influent water in the tanks where the water is aerated so that the microbes utilize the pollutants, while sedimentation removes the indigestible solids. In the secondary treatment the water, which has already received a primary treatment, is filtered through soil where it also receives treatment by soil organisms. In addition there is transpiration of water and absorption of minerals by plants. In the primary treatment BOD, SS, coliforms (E. coli), TP and total bacteria were removed 79-99%, but COD and TN were removed only 58% and 36%, respectively. In the secondary treatment removal of nutrients proceeded further, and 93-99% of pollutants were removed. The treated waters met the quality standard of discharge water in Japan except for TN, which was in too great a concentration to meet discharge standards. This problem requires further study.

• Journal of Microbiology and Biotechnology
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• v.3 no.1
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• pp.19-23
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• 1993

Spirulina platensis NIES 39 was grown in effluents from a wastewater treatment plant utilizing activated sludge process at a pig farm to reduce pollutants and to produce feed resources. The addition of 2 g/l NaCl was required for the growth of S. platensis in the effluents with about 100 mg/l ammonia nitrogen concentration. The growth was stimulated by the addition of 2 g/l sodium bicarbonate and 4 mg/l ferrous sulfate. The microorganism grew well at pH 8~11, and the optimum pH for the growth was 10. The algal concentration of 1.1 g/l was attained after 8 days of growth at the optimum condition with the removal of 95% ammonia nitrogen and 34% phosphorus.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.167-170
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• 2000

Proteolytic hydrolysis is one of the main enzymatic reaction of waste activated sludge (WAS) digestion. Pretense excreted from Bacillus stearothermophilus (ATCC 31197) showed optimum temperature of $75^{\circ}C$ for maxium heat stable proteolytic activity against azo casein. The dependency of $Ca^{2+}$, $Zn^{2+}$ on heat stability of proteolytic enzymes were measured with various concentrations. It was shown that $Ca^{2+}$ ion enhanced heat stability of these enzymes. Then thermophilic aerobic digestion (TAD) was performed using B. sterothermophilus with the addition of divalent ions. Performance of TAD process with ATCC 31197 activated by $Ca^{2+}$, $Zn^{2+}$ions in terms of dissolved organic carbon (DOC) concentration, extracellular protein concentration, and scanning electrion microscopy (SEM) analysis. The best result of protein reduction concentration in digestion test was obtained with the addition of 2 mM $Ca^{2+}$ ion.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.39 no.2 s.120
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• pp.9-16
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• 2007

Biological treatment using the activated sludge method and biofilm process has been developed for paper wastewater treatment. It is known that a water treatment using biofilm process has a high efficiency be-cause a great deal of microorganism could adhere to media. It is also known that various plastics such as polyurethane and polyethylene have been used as the media. In this study polyethylene was used as a media and rice straw an additive agent to improve porous and hydrophilic properties of the media for waste water treatment. Porosity and hydrophilic characteristics of polyethylene was increased as rice straw was added to polyethylene. Paper wastewater was then treated with newly developed environment materials. Rice straw showed excellent results in waste water treatment in various media. This environmentally friendly material prepared by polyethylene and rice straw could show similar results to those of a commercial porous polyurethane foam in wastewater treatment.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.434-437
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• 2003

A new bacterial strain, was isolated from activated sludge, identified and named P. aeruginosa EMS1. The new strain produced surface-active rhamnolipids by batch cultivation in mineral salts medium with waste flying oils. The mutant strain KH7, designated P. aeruginosa EMS1, derived by random mutagenesis with N-methyl-N-nitro-N-nitrosogoanidine treatment producing high levels of the biosurfactants was selected by an ion-pair plate assay. The mutant strain KH7 showed 4-5 times more hydrocarbon emulsification as compared to the parent when grown on waste frying oils and various hydrocarbons. Furthermore, P. aeruginosa EMS1 and mutant strain KH7 was also able to use whey as a co-substrate for growth and biosurfactant production. As results of this study, mutant strain KH7 is a very efficient biosurfactant producer, and its culture conditions are relatively inexpensive and economical. Rhamnolipid is synthesized by the rhlAB-encoded rhamnosyltransferase. To be convinced of these genes, we performed PCR based on P. aeruginosa PAO1 whole-genome database. rhl gene cluster nucleotide and amino acid sequences were compared for both parent and mutant. Comparison of nucleotide sequence of rhlAB, there were usually terminal's codons exchange.