• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.2
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• pp.146-157
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• 1994

Wasong(Orostachys japonicus A. Berger) has been used as anti-cancer medicinal plants from ancient time. This experiment was conducted to obtain some fundamental informations concerning anatomical study, germination test, soil analysis of indigenious districks, adaptaion of soil for wasong. The leaf of wasong was xerotype with spine, mesophyl chloroplast is dense surrounded vascular bundle, with a many large water storage tissue without chloroplast, and was bifacial stomata leaf type. The stem of wasong was amphicribral vascular bundle, central cylinder was cylinder type and protostele. Root was polarch type, potoxylem of it's was arranged in ring shape of annulation and metaxylem. The structure of flower was cymose, was caylyx of 5 sepals, corolla of 5 petals, roecium of 10 stames, ovules of five, placentation parietal, ovary superior, axile placenta. The optimum temperature for seed germination was $25^{\circ}C$ under light and germination percentage was 22.5%. Dormancy breaking was effective for 6-8 days at 5$^{\circ}C$ and at 100 ppm level of $GA_3$ The soil analysis of wasong local districks, content of available $P_2O_5$, Ca were higer than optimal level of upland and C.E.C., exchangeable k was not significantly different of that, but exchangeable magnesium was very low. The growth of wasong was affected draining regardless soil combination treatment.

• Herbal Formula Science
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• v.25 no.2
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• pp.135-143
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• 2017

Objectives : This trial aimed to determine if Wasong (Orostachys japonicus) extract can enhance immune system and is safe enough to be approved as a health functional food. Methods : Total 62 people, aged 45 and older, will be recruited to participate in a randomized, double-blind, placebo-controlled clinical trial. This study will compare Wasong extract and placebo. Wasong group will take 1g of Wasong extract, once a day, for 8 weeks. Placebo group will take 1g of crystalline cellulose as placebo, once a day, for 8 weeks. Outcomes will be measured at the baseline, the end of 4th week, and 8th week. Primary outcomes are the ratio of NK cells/total lymphocytes and the ratio of T-helper cells/T-suppressor cells. Secondary outcomes are total white blood cell count, the ratio of neutrophils, lymphocytes, and monocytes in total leukocytes, the ratio of total T cells, T-helper cells, T-suppressor cells, and B cells to lymphocytes, the amount of blood IgM, IgG, IgA, and cytomegalovirus (CMV) IgG, and blood metabolite target &global analysis. Results : This trial was approved by institutional review board of Pusan National University Korean Medicine Hospital (registry number: 2016006), and registered in Clinical Research information Service, one of WHO International Clinical Trials Registry Platform (registry number: PRE20161006-002). Recruitment opened in February 2017 and is supposed to be completed by August 2017. The result is expected to be published by June 2018. Conclusion : This trial will provide clinical information to determine the efficacy and safety of Wasong in enhancing immune system of middle-aged and older people.

• The Journal of Internal Korean Medicine
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• v.29 no.3
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• pp.582-594
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• 2008

Objective : The purpose of our study was to investigate the effect of JinlnWhaChul-tang-ga-wasong (JIN) on NDEA-induced liver tumorigenesis. Materials and Methods : We investigated the possible protective effects of Jininwhachul-tang-ga-wasong (JIN) as an anticancer against NDEA-induced liver injury in mice. Experimental mice were classified into 3 groups; normal, saline administered group (control group), and JIN extract (0.15g/kg/every other day) administered group (JIN group) after being injected with NDEA over 12 weeks. We examined the state of differentiation of these tumors and the effects of JIN after 6 weeks. To confirm the induction of apoptosis, the cells were analyzed by terminal deorynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay, acridine orange staining and flow cytometric analysis. To investigate inhibitory effect on the expression of COX-2 by JIN, we performed COX-2 immunohistochemistry and reverse transcription polymerase chain reaction analysis. Results : Body weights significantly decreased in the control and JIN groups compared with the normal group. The levels of cholesterol, hemoglobin and testosterone decreased in the control compared with the normal group. The level of estradiol significantly increased in the control compared with the normal group. The control group reacted with TUNEL assay more than the normal and JIN groups. Upon naked eye, light and electron microscopic examination, JIN improved the morphological and histopathological changes of the liver caused by NDEA-induced hepatic neoplasm. COX-2 immunoreactivity decreased in the JIN group compared with the control group, mRNA expression of the control group was greater than the normal and JIN groups. Conclusion : these results suggest the possibility that JIN may exert an anti-tumor effect on NDEA-induced liver tumorigenesis.

• The Korea Journal of Herbology
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• v.31 no.4
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• pp.93-100
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• 2016

Objectives : The internet is the most common method to investigate information. It is showed that 75.2% of Internet users of 20s had health information search experience. So this study is aim to understanding of interest of public about the herbal medicine using internet search query volume data.Methods : The Naver that is the top internet portal web service of the Republic of Korea has provided an Internet search query volume data from January 2007 to the current through the Naver data lab (http://datalab.naver.com) service. We have collected search query volume data which was provided by the Naver in 606 herbal medicine names and sorted the data by peak and total search volume.Results : The most frequently searched herbal medicines which has less bias and sorted by peak search volume is 'wasong (와송)'. And the most frequently searched herbal medicines which has less bias and sorted by total search volume is 'hasuo (하수오)'.Conclustions : This study is showed that the rank of interest of public about herbal medicines. Among the above herbal medicines, some herbal medicines had supply issue. And there are some other herbal medicines that had very little demand in Korean medicine market, but highly interested public. So it is necessary to monitor for these herbal medicines which is highly interested of the public. Furthermore if the reliability of the data obtained on the basis of these studies, it is possible to be utilizing herbal medicine monitoring service.

• Korean Journal of Medicinal Crop Science
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• v.13 no.3
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• pp.114-117
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• 2005

Orostachys japonicus, called Wasong as herbal medicine and a short day plant, should be artificially kept in long daylength to control anthesis of its florets. The study was done to clarify the effect of daylength (10, 13, 16 hours a day) on growth, morphological characters, and flowering. The treatments were done on August 25 and afterward samples were taken every 2 weeks. Growth, morphological and flowering related characters were measured from each sample. With longer daylength plant height and inflorescence length were increased but number of leaves including bracts and stem diameter were decreased. Leaves and bracts, stem, root, shoot and total dry weights were increased with longer daylength from late September while floret dry weight showed reverse result. Florets formed were the greatest in daylength of 10 hours during September but the least during the other period. Anthesis of the florets was observed only in daylength of 10 hours during October and all the plants from the treatment were flowered.

• The Journal of Internal Korean Medicine
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• v.32 no.2
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• pp.153-169
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• 2011

Objectives : This study was performed to investigate the anti-fibrogenic effect and the effect on cell growth and apoptosis in YJCGT, YJCGT YSO and YJCGT YSCO on thioacetamide-induced rat liver tissue and the immortalized human hepatic cell line LX2. Materials and Methods : LX2 cells were treated with various concentrations (0, 50, 150, 300 ug/ml) of YJCGT, Y+YSO, and Y+YSCO extract for 24, 48 and 72 hours. After the treatment, cell viability was measured by using MTT assay. Caspase inhibitor assay, and cell viability were determined by a colorimetric assay with PMS/MTS solution. Rat liver fibrosis was induced by intraperitoneal thioacetamide injection 150 mg/kg 3 times a week for 5 weeks. After the treatment, body weight, liver & spleen weights, liver function test, the complete blood cell count and the change of portal pressure were studied. After YJCGT, Y+YSO, and Y+YSCO treatment, percentages of collagen in thioacetamide-induced rat liver tissue were measured. Results : The viability of the LX2 cell decreased in a dose- and time-dependent manner. Exposure of LX2 cells to YJCGT, YJCGT+YSO and YJCGT+YSCO induced caspase-3 activation, but co-treatment of YJCGT, YJCGT+YSO and YJCGT+YSCO with the pan-caspase inhibitor Z-VAD-FMK, and the caspase-3 inhibitor Z-DEVE-FMK, blocked apoptosis. There was no difference in rat body weight between the thioacetamide only group and the YJCGT, YJCGT+YSO and YJCGT+YSCO groups. In the YJCGT, YJCGT YSO and YJCGT YSCO groups, the serum level of GPT significantly went down compared with the thioacetamide only group. In the YJCGT, Y+YSO, Y+YSCO groups, white blood cell elevated by thioacetamide injection decreased but RBC, Hgb, and Hct increased. In the Y+YSO group, the portal pressure elevated by thioacetamide injection significantly decreased. In the histological finding, thioacetamide injections caused severe fibrosis, but YJCGT, Y+YSO, and Y+YSCO treatment significantly reduced the amounts of hepatic collagens. Conclusions : YJCGT, Y+YSO, and Y+YSCO inhibit the growth of LX2 cells by inducing apoptosis through caspase activity. YJCGT, Y+YSO, and Y+YSCO have beneficial effects on the treatment of cirrhotic patients as well as patients with chronic hepatitis.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.5
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• pp.600-607
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• 1995

Orostachys japonicus, called Wasong and used as anti-tumor medicinal plant, was cultivated in plastic house. The experiment was done to clarify the effect of long-day and night-break treatment at the timing of bolting on its morphological characters, organ dry weight and flowering of florets. After grown in 15cm plastic boxes containing 2:1 soil:peat moss mixture for about 4 months, long-day of 16 hours and night-break of 2 hours around midnight were treated from Sept. 9. The plants were sampled 5 times at 2-week interval after the treatments. Long-day and night-break treatment delayed the growth of inflorescence and showed greater stem diameter on the last sampling and no. of leaves and bracts than the natural daylength. The treatments also had greater leaf and bract dry weight since 2 weeks, and the other fraction and total dry weights since 4 weeks but less floret dry weight from 4 to 6 weeks after the treatments than the natural daylength. The treatments, however, decreased no. of flowered florets and ratio of flowering plants although all the treatments showed nearly the same no. of total florets per plant until 6 weeks after the treatments, late October, which resulted in the modification of source to sink or vice versa. In the natural daylength, the florets were functioned as sink, while root, leaf and bract as source, but in the long-day and night-break treatments stem and florets were done as sink.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.2
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• pp.236-242
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• 1996

Orostachys japonjcus, called Wasong in herb medicine, has been artificially cultivated as an anti-tumor medicinal. The experiment was done to examine the effect of night-break periods imposed immediately before its bolting time on its morphological, flowering-related characters and fraction dry weights. After a plant was grown in a 15cm plastic pot containing a 2:1 soil:Peat moss mixture for about 3 months, three different night-break periods (0.5, 1 and 2 hours) around midnight were treated from Aug. 24. to compare with the natural daylength. The plants were sampled 6 times by 2-week interval after the treatments. Plant height and inflorescence length of all the treatments inclined with time lapse after the treatment and were shorter in 2 hour night-break due to slow increment than in the other treatments, while stem diameter showed reverse result. All the treatments except 2 hour night -break were nearly same in fraction, shoot and total dry weights per plant; two hour night-break treatment had greater leaf and bract weight from 6 weeks, greater stem, shoot and total weights from 2 weeks and greater root weight from 4 weeks but did less floret weight after the treatment. Although florets on the inflorescence were formed in 2 hour night-break treatment, flowering florets and flowering plants never occurred. In the other treatments showed the similar response, however, more florets appeared from 2 weeks, flowering florets was sharply increased from 6 weeks and flowering plants were reached up to 100% from 6 to 8 weeks after the treatment. Inflorescence length or number of total florets per plant in 2 hour night-break was positive-correlated to all the fraction dry weights except that those of natural daylength was not done, meaning that its artificial cultivation should permit bolting to secure more shoot dry matter.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.5
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• pp.597-603
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• 1997

Orostachys japonicus, Wasong as herb medicine, has been artificially cultivated as an anti-tumor medicinal. The experiment was carried out to examine the effect of natural daylength as control or night-break treatment (NB) imposed at June 20, July 18 or Aug. 15 on its growth, dry weights of leaf and bract, stem, floret and root, and morphological characters including bolting and floret flowering. After a plant was grown in a 15cm plastic pot containing a 2 : 1 soil : peat moss mixture on May 23, three treatments with above differing night-break had been imposed around midnight up to Nov. 7. The plants were sampled 3 times at the same day forced to night-break and then done 6 times by 2-week interval after the final NB. Plant height and inflorescence length of all the NB increased with delayed NB but declined in comparison with the natural daylength. No. of leaves including bracts showed similar response to plant height although NB given before July 18 showed less leaves and bracts. Stem diameters of NB were continuously increased to middle Sept. to middle Oct. while that of natural daylength decreased after middle Oct. Natural daylength or NB given on Aug. 15 had greater fraction, shoot and total dry weights resulting from increment of leaf and bract up to Aug. or of floret, stem and root after Sept. The earlier NB, the later formation of florets and the less number of flowering florets whereas in natural daylength florets on inflorescence begun to be formed from middle Sept. were sharply increased up to middle Oct. when all the plants were flowered. Bolting was not formed in the plant of the earliest NB of June 20, and thereby no anthesis of florets up to early Nov. It was concluded that year-round cultivation of Orostachys japonicus plants was possible through controlling the NB timing because its bolting and flowering of florets separately occurred.