• Journal of Korean Society on Water Environment
• /
• v.31 no.5
• /
• pp.523-532
• /
• 2015

Hydrocyclone is widely used in industry, for its simple design, high capacity, low maintenance and low operational cost. The objective of this study is to develop hydrocyclone coagulation and filtration system. The system is made of hydrocyclone ballasted coagulation with polyaluminium chloride silicate (PACS) and upflow filter to treat micro particles in urban storm runoff. Roadside sediment particles (< $200{\mu}m$) was mixed with tap water to make various turbid suspensions to simulate urban storm runoff. The filter cartridge was filled with polyethylene media system and ran 1hr per everyday and total operation time were 8.19hrs and backwashing everyday after end of operation. The operation condition of flowrate was $8.2{\sim}11.9m^3/day$ (mean $10.1m^3/day$) and surface overflow rate (SOR) based on filter surface area was $45.5{\sim}65.9m^3/m^2/day$ (mean $55.7m^3/m^2/day$). The range of PACS dosage concentration was 14.0~31.5 mg/L. As the results of operation, the range of removal efficiency of turbidity, SS were 81.0~95.8% (mean 89.5%) 81.8~99.0% (mean 91.4%), respectively. An increase of filtration basin retention time brought on increased of removal efficiency of turbidity and SS, and increase of SOR brought on decreased of removal efficiency. During the first flush in urban area, storm runoff have an high concentration of SS (200~600 mg/L) and the filtration bed becomes clogged and decreased of removal efficiency. Backwashing begins when the drainage pipe valve at the filtration tank bottom is completely open (backwashing stage 1). Backwashing stage 2 was using air bubbles and water jet washing the media for 5 mins and open the drainage valve. After backwashing stage 1, 2, 61.83~64.04%, 18.53~27.51% of SS loading was discharged from filtration tank, respectively. Discharged SS loading from effluent was 7.12~14.79% and the range of residual SS loading in fliter was 2.26~5.00%. The backwashing effects for turbidity, SS were 89.5%, 91.4%, respectively. The hydrocyclone coagulation and filtration with backwashing system, which came out to solve the problems of the costly exchange filter media, and low efficiency of removing micro particles of filter type nonpoint treatment devices, is considered as an alternative system.

• Korean Journal of Microbiology
• /
• v.21 no.4
• /
• pp.221-228
• /
• 1983

An improved procedure for the rapid purification of glucose-6-phosphate dehydrogenase from extracts of Saccharomyces cerevisiae was developed by using affinity chromatography. Among six affinty media tested, $NADP^+ -agarose$ and Affi-gel Blue were more effective than others (i.e., Affi-gel Red, AMP-agarose, ATP-agarose, and $NAD^+ -agarose$). Conditions to desorb the enzyme bound to the affinity media were examined to increase the purity as well as yield. The best result was obtained when the column was developed with a linear gradient of KCl (0-1.0M). In case of Affi-gel Blue, introduction of $NAD^+$ (15mM) washing step prior to the salt gradient was most effective to remove $NAD^+ -binding$ proteins. For a large scale preparation of G-6-P dehydrogenase higher recovery was obtained by Affi-gel Blue than $NADP^+ -agarose$, however, the purity of the enzyme was decreased by 10 times if the former was used as the affinity medium. The capacity of Affi-gel Blue for G-6-P dehydrogenase was found to be 5 times higher than that of $NADP^+ -agarose$. Furthermore Affi-gel Blue could be reused repeatedly and its preparation is relatively easier and less expensive than $NADP^+ -agarose$.

• Journal of Naturopathy
• /
• v.8 no.2
• /
• pp.71-77
• /
• 2019

Purpose: The purpose of this study was to investigate the far-infrared emissivity of patented ocher quilt cotton fabrics and to investigate the microorganisms that survived the washing of cotton fabrics up to 20 times. Methods: A 16S rRNA assay was performed using a far-infrared radiometer and a single colony in which microorganisms grew in nutrient media. Results: The far-infrared emissivity of ocher quilt was 0.902 (90.2%) at 5~20 ㎛ at 40℃, and the radiation energy was 3.63 × 10² w/m². The number of viable cells was 2.0 × 10² cells/ml in ocher duvet cotton fabric, and no viable bacteria found in regular cotton fabric. The base sequence of 16S rRNA of B-2 strain isolated into single colonies was 1,419 bases, and the base sequence of strain A-4 was 1,284 bases. The base sequence of 16S rRNA of these two strains showed high homology with Bacillus spp. The B-2 bacteria showed high homology with 99.0% of the 16S rRNA sequence of B. aryabhattai EF114313 and 99.0% of the A-4 bacteria of B. bingmayongensis AKCS01000011. Consequently the colony strain B-2 finally identified as B. aryabhattai BJ-2 and A-4 as B. bingmayongensis BJ-4 strain. Concusions: Soil Bacillus strains survived in ocher quilt cotton fabric after 20 washing. The material can be useful because quilt cotton fabric emits a large amount of far-infrared and far-infrared radiation energy.

• Journal of Microbiology and Biotechnology
• /
• v.12 no.2
• /
• pp.196-203
• /
• 2002

A novel process for urokinase purification was studied using p-aminobenzamidine as the ligand and sepharose 4B as the matrix. The adsorption, washing, and elution conditions were optimized by an unusual method. An adsorption buffer containing 2.5 M NaCl and $1\%$ Tween 80 facilitated the adsorption of urokinase on the affinity media and prevented contaminants from binding to the p-aminobenzamidine affinity gel. It was found that $5\%$ Tween 80 removed most of the contaminants from the affinity column. A 0.2 M glycine elution buffer containing 0.5 M NaCl (pH 3.0) was found to have a strong elution ability with a high recovery and purity of urokinase. A crude urokinase material of231 IU/mg protein from human urine was purified to 124,300 IU/mg protein with a purification factor of 538 and yield of $86.7\%$. As a result, a high purity urokinase was obtained with only a single affinity chromatography step. The purification process was successfully scaled-up to a 2-1 chromatography column. The resulting urokinase eluate could be directly lyophilized, thereby complying with Chinese pharmacopoeia (1995 version) standards.

• Hwankyungkyoyuk
• /
• v.9 no.1
• /
• pp.58-71
• /
• 1996

The purpose of this study was to investigate the rural housewives' recognition and practice on environmental preservation. The subjects of this study were 300 rural housewives living in Chung-buk province. Questionaires were distributed to them during a week from August $25^{th}$ to August $31^{th}$ in 1995. Major findings were as follows: 1. The saved water quite well in washing their faces and the dishes compared with urban housewives, but they did not save water so well in using the lavatory. They used synthetic detergent, one of water pollutants, more often than natural soaps in shampooing and laundering. In addition, they did not use a measuring cup. It resulted in the waste of detergent and aggravation of water pollution. 2. Food waste was disposed of properly, but used batteries were not collected separately. A disposable vinyl bag was more commonly used than a reusable shopping basket. The reason for not using a shopping basket was bothering or uncustomary. Since most of them did not know refillable products of goods with environmental mark, they did not use those. Therefore, housewives should be educated in order to prevent soil pollution. 3. The use of spry-type pesticide and hair treatment, one of air pollutants, was wide-spread among them. They hardly recognized and practiced saving of electric power when they used household electric appliances. 4. The major source of environmental knowledge was mass media like TV of radio. Many of them did not know the practical ways to solve the environmental education program. In conclusion, low level of recognition and practice of rural housewives on environmental preservation indicated that a systematic environmental education program should be developed and implemented.

• Journal of the Korean Home Economics Association
• /
• v.27 no.4
• /
• pp.85-103
• /
• 1989

The purpose of this study is to figure out the urban low-income consumers' present possesion condition of electric appliances, the way of purchasing, shopping scope of groceries and electric appliances, and the shopping scope and information seeking behavior of groceries and electric appliances according to the socioeconomic variables, and the shopping scope of electric appliances according to the way of paying price. For this purpose 129 low-income consumers living in Inchon were interviewed through questionnares. Finally 100 questionnares were analyzed. The first major of this study is that the possesion rates of color television set and refrigerator are comparativelyl high, while those of washing machine, record player and camera are low. In case of electric appliances, consumers usually purchase things on credit and as a shopping place, they usually go to a commercial agent. In case of groceries, they usually buy things for cash, and in case of one price labeled groceries they usually buy things at a supermarket. Second, according to the way of paying price, the shopping scope of some electric appliances is broader in case of purchase for cach than in case of purchase on credit. Third, the shopping scope and information seeking behavior classified by the socioeconomic variables according to the income, age and education. That is as the income and education become higher and the age becomes lower, the shopping scope becomes broader, mass media information source is much more uaed and the frequency of seeking becomes higher. Through this study, I would like to suggest the followings. (1) The problem of low-income consumers is closely connected with the low education level, so it can be solved a bit through consumer education. (2) Especially the consumers' problem is more serious in case of those who are aged and get very low income and education. Therefore special attention is needed to those consumers.

• Journal of Technologic Dentistry
• /
• v.31 no.3
• /
• pp.21-26
• /
• 2009

Purpose: Standards of alloy for porcelain fused to metal crown be classified by metallic factor and biological factor. Metallic factors consist of stability of alloy composition and mechanical strength and surface characteristics for chemical bond. Biological factors be considered properties of metallic elements and problems originated by toxicity and hypersensitive reaction. Alloys considered such controversial points are the most suitable alloy for dental instrument. Method: Alloys added Be and Nb using Ni-Cr alloy which has been widely used for dental instrument be selected and classified experimental group. Non-addition Be and Nb to Ni-Cr alloy classify control group and addition Be alloy is Be-experimental group, addition Nb alloy is Nb-experimental group. Specimens for cytotoxicity analysis gave effect to washing and sterilization. and then made an experiment on elution with cell medium after disinfection. It conducted specimens within cell medium with 24hours, 48hours, 72hours, respectively. It cultured human dermal fibroblast(HDF) using cell medium for cytotoxicity test and then investigated elution rate through spectroscopic analysis by MTT-assay. Result: As results of cytotoxicity test by MTT-assay, cultured cell rate of VII measured more low numerical value within elution medium for 24hours focused on control group. Also, cultured cell rate of K3 alloys observed low value for 48hours, 72hours than value of control group. Conclusion: According to final result that synthesize above results, Ni-Cr alloy added Be and Ni has little difference in Cytotoxicity by MTT-assay.

• Journal of Korean Society of Water and Wastewater
• /
• v.32 no.6
• /
• pp.507-515
• /
• 2018

The results of the survey on water utilization status, the wasted water factor, the awareness, and the education and promotional activities of water saving in Daejeon City are as follows: The percentage of using tap valve with a closure was 66% when showering. 74% of water was used with closing the tap valve when washing faces with no water needed, it was still considered that there existed a wasting water. The most wasting reasons of water were found to be shower, bath, and laundry in order. In the awareness of practicing tap water saving, 9.1% of respondents acted actively, 53.4% of them were trying to practice it, and more than 63% had a positive awareness. In the contents of practicing saving water, locking water when soaping while showering, non-laundry of small quantity, and using cups when brushing teeth were top priorities. About 50% of the reasons for saving water was to practice purely saving water, when saving for economic help was excluded. The level of water saving awareness was not high when considering that the ratio of usage without water saving equipment and installation, and using method was relatively high when moving in. In order to make usage of water saving equipment more common, it was deemed necessary to provide information on the purchase of water saving equipment and to promote and educate the efficiency of saving of related products. More than about 90% of the respondents responded that water saving education or promotional activities had an effect of improving water saving awareness. The most effective method of education and promotional activities was found to be 40.4% by mass media activities, and 21.7% by promotions through mobile devices.

• Journal of Life Science
• /
• v.16 no.2 s.75
• /
• pp.289-296
• /
• 2006

In order to establish highly efficient gene transfer condition at early stage of soybean transformation, various experiments were performed and compared their efficiencies by transient GUS analysis; those conditions are genotype determination of Korean soybean cultivars for amenability to Agro-infection, appropriate agar and selective agent concentration, orientation of explant placement, hormone pre-culture, and liquid selection condition. In the genotype screen of Korean soybean varieties, 14 amenable genotypes were selected. For efficient Agrobacterium washing, cefotaxime was chosen and hygromycin at the concentration of 10 and 15 ppm was used as selection agent in the media. Agar concentration was slightly better in 0.6% and 0.8% for both shoot and callus formation, and explant placement with adaxial side down showed high frequency of GUS expression. For wounding treatment, oriental needle was efficient than scalpel for shoot formation and gene transfer. To increase the frequency of gene transfer, hormone pre-treatment was applied. BA at the concentration of 5 and 10 ppm resulted in better survival at the late stage of selection in shoot elongation media. Selection in liquid media after hormone pre-treatment seemed to be effective to remove the escaped non-transformants at early stage of procedure. Considering the results obtained, Eunhakong could be the first choice as a material for soybean transformation among Korean soybean genotypes.

• Journal of Environmental Health Sciences
• /
• v.30 no.4
• /
• pp.329-343
• /
• 2004

We conducted to investigate citizen's attitude to the treatment of food waste in Yongin city. The respondent of $41.81\%$ throws the food waste eliminate from home and store less than 1l in Yongin. When they dump the food waste, they speak out the inconvenience of damaged envelope by animals in case of home and store. This caused troubles for reason of sanitary. So a local autonomous entity must carefully consider of expand use specially designed container as like apartment house. They give an answer that the collecting time of food waste is suitable form dawn till morning. This answer shows the satisfaction of the period time to collect the food waste. They prefer to be appointed the exclusive place to collect food waste. The service interval of collect is suitable 1 time a day. They want to increase the number of washing of the collecting container. This is good method for sanitary condition, but the care of the period time to collect the food waste is more efficient than the care of the number of washing the collecting container. The care of the period time minimizes to incur the enmity of the people and to pollute in environment. The major of respondent handled the food waste after keeping the basket or a kit. This fact shows to us almost citizen doesn't feel the seriousness to remove the moisture of the food waste. Recently, many solutions which can be disposal efficiently are getting magnified and improved owing to increase utilities channel to loss in quantities and dry the food waste. We expect the reduction of food waste is solved getting easily step by step. The results of the awareness about the facility of food waste show citizen prefer recycling facility to the other facilities. If recycle facility and incineration facility are constructed, they were worried about bed smell. When some facility of the food waste is constructed, they have to maintain and to handle not to incur the enmity of the people. The spread rate of specially designed container already increased, the citizen set a high value on the use of specially designed container more than amount-rate vinyl envelope that people have used for several years. In the cost treatment about food waste, the major respondent answered the use cost of specially designed container is suitable price. So we can know the use charge is proper level. The majority of citizen more prefer autonomous plan which voluntary atmosphere creation and public information by mass media than levy system and rising treatment cost which forced plan. The citizens have pretty positive thinking of incineration, so the government needs more efforts for a public notice, which includes the incineration is no more than abandoned thing. Each of local self government has to sort the food waste and make kind of resource system related to collecting and carrying, constructing a suitable facility, proper disposal of the food waste and producing harmless in our surrounding in order to solve the invisible problems. To do above mentioned things, we have to analyze referred several problems till now. Also, to minimize the side effect, the government will have to improve through enforce the system.