• Korean Journal of Pharmacognosy
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• v.48 no.4
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• pp.339-342
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• 2017

Quercus mongolica (QM) is a species of Quercus native to Eastern Mongolia, Siberia, China, Japan, and Korea. QM has been used as Korean traditional medicine for the treatment of inflammation of the skin. Present work, the validation and content determination of the phenolic compounds which were isolated from the leaves of QM, including pedunculagin (1) and kaempferol-3-O-(6"-galloyl)-${\beta}$-D-glucopyranoside (2) were conducted through High-Performance Liquid Chromatography (HPLC). As a result, the contents of these compounds were 4.14% and 0.76% in QM extract, respectively.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.20
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• pp.8651-8656
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• 2014

Purpose: To study the expression of angiogenin-2 (Ang-2) and its receptor Tie-2 in colorectal cancer and discuss the possible mechanisms behind this process. Materials and Methods: Using the streptavidin-peroxidase (SP) immunohistochemical method, paraffin sections from 100 colorectal cancer samples and 10 samples from tumor-adjacent normal tissue (> 2 cm from the edge of the gross tumor) were tested for protein expression of Ang-2, Tie-2, PI3K, and AKT. Reverse transcription-polymerase chain reaction and Western blots were further used to measure expression of the 4 genes and proteins in 20 freshly-resected colorectal cancer samples and tumor-adjacent normal tissues. Results: In colorectal cancer tissues, the expression of the Ang-2, Tie-2, PI3K, and AKT genes and their proteins was significantly higher than in tumor-adjacent normal tissues. Protein expression in poorly-differentiated adenocarcinoma was higher than that in well and moderately differentiated adenocarcinoma. According to Duke's classification, the protein expression in Stages C and D was significantly higher than that in Stages A and B. In the group with lymphatic metastasis, the protein expression was higher than that without lymphatic metastasis. Conclusions: In colorectal cancer, the expression of the Ang-2, Tie-2, PI3K, and AKT genes and their proteins is markedly higher than those in tumor-adjacent normal tissues. No correlation was observed between protein expression and gender, location, or histologic type. Correlations did exist between protein expression and differentiation level, stage of Duke's classification, and lymphatic metastasis; in colorectal cancer tissues with lower differentiation levels, higher stages of Duke's classification, and lymphatic metastasis, the expression of all 4 proteins was higher. The study of their expression patterns and relationships with aggression and metastasis will provide a valuable experimental foundation for assessing prognosis and targeted therapy of colorectal cancer.

• Journal of Ginseng Research
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• v.47 no.2
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• pp.218-227
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• 2023

Background: Myocardial fibrosis (MF) is an advanced pathological manifestation of many cardiovascular diseases, which can induce heart failure and malignant arrhythmias. However, the current treatment of MF lacks specific drugs. Ginsenoside Re has anti-MF effect in rat, but its mechanism is still not clear. Therefore, we investigated the anti-MF effect of ginsenoside Re by constructing mouse acute myocardial infarction (AMI) model and AngII induced cardiac fibroblasts (CFs) model. Methods: The anti-MF effect of miR-489 was investigated by transfection of miR-489 mimic and inhibitor in CFs. Effect of ginsenoside Re on MF and its related mechanisms were investigated by ultrasonographic, ELISA, histopathologic staining, transwell test, immunofluorescence, Western blot and qPCR in the mouse model of AMI and the AngII-induced CFs model. Results: MiR-489 decreased the expression of α-SMA, collagenI, collagen III and myd88, and inhibited the phosphorylation of NF-κB p65 in normal CFs and CFs treated with AngII. Ginsenoside Re could improve cardiac function, inhibit collagen deposition and CFs migration, promote the transcription of miR-489, and reduce the expression of myd88 and the phosphorylation of NF-κB p65. Conclusion: MiR-489 can effectively inhibit the pathological process of MF, and the mechanism is at least partly related to the regulation of myd88/NF-κB pathway. Ginsenoside Re can ameliorate AMI and AngII induced MF, and the mechanism is at least partially related to the regulation of miR-489/myd88/NF-κB signaling pathway. Therefore, miR-489 may be a potential target of anti-MF and ginsenoside Re may be an effective drug for the treatment of MF.

• Journal of the Korean Institute of Intelligent Systems
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• v.25 no.1
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• pp.97-104
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• 2015

An 'associative cube', a class of auto-associative memories, is revisited here, in which training data and hidden orthogonal basis functions such as wavelet packets or Fourier kernels, are combined in the weight cube. This weight cube has hidden units in its depth, represented by a three dimensional cubic structure. We develop an unsupervised incremental learning mechanism based upon the adaptive least squares method. Training data are mapped into orthogonal basis vectors in a least-squares sense by updating the weights which minimize an energy function. Therefore, a prescribed orthogonal kernel is incrementally assigned to an incoming data. Next, we show how a decoding procedure finds the closest one with a competitive network in the hidden layer. As noisy test data are applied to an associative cube, the nearest one among the original training data are restored in an optimal sense. The simulation results confirm robustness of associative cubes even if test data are heavily distorted by various types of noise.

• The Korean Journal of Physiology and Pharmacology
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• v.22 no.2
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• pp.127-134
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• 2018

Myofibrillogenesis regulator-1 (MR-1) is a novel protein involved in cellular proliferation, migration, inflammatory reaction and signal transduction. However, little information is available on the relationship between MR-1 expression and the progression of atherosclerosis. Here we report atheroprotective effects of silencing MR-1 in a model of Ang II-accelerated atherosclerosis, characterized by suppression focal adhesion kinase (FAK) and nuclear factor kappaB ($NF-{\kappa}B$) signaling pathway, and atherosclerotic lesion macrophage content. In this model, administration of the siRNA-MR-1 substantially attenuated Ang II-accelerated atherosclerosis with stabilization of atherosclerotic plaques and inhibited FAK, Akt, mammalian target of rapamycin (mTOR) and NF-kB activation, which was associated with suppression of inflammatory factor and atherogenic gene expression in the artery. In vitro studies demonstrated similar changes in Ang II-treated vascular smooth muscle cells (VSMCs) and macrophages: siRNA-MR-1 inhibited the expression levels of proinflammatory factor. These studies uncover crucial proinflammatory mechanisms of Ang II and highlight actions of silencing MR-1 to inhibit Ang II signaling, which is atheroprotective.

• The Korean Journal of Physiology and Pharmacology
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• v.19 no.5
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• pp.473-478
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• 2015

To see the inhibitory mechanism of gentamicin in response to electrical field stimulation (EFS) using the rat bladder smooth muscle, atropine or guanethidine was treated but had no effect. Methylsergide, a non-selective 5-$HT_1$, 5-$HT_2$ receptor antagonist was also treated but had on effect. Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect. For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS. Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor. The inhibition of gentamicin might be mediated through the PLC dependent pathway, but not through the PKC, MLCK or rho kinase dependent pathway.

• Proceedings of the KAIS Fall Conference
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• 2010.05b
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• pp.634-636
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• 2010

소비자의 행동이론은 경영학의 주된 관심사이다. 소비자의 소비 행동패턴 이론은 소비자의 합리적 소비에 근거한 선호도로써, 선택의 문제를 주요한 연구대상으로 삼고 있다. 하지만 본 모델링은 소비자의 선택을 선호의 문제로 취급하는 기호에 의존하기 보다는, 상권의 측정가능한 크기에 의존하는 수동적 의미의 소비패턴을 산정하게 된다. 단순한 몇 가지 가정을 기반으로 하여 상권이 미치는 영향력을 상권의 거리와 상권의 크기로만 한정할 경우, 최적 경로에 따른 합리적인 소비함수 모델링을 만들 수있다. 상권의 거리 및 상권의 크기로 상권의 범위를 제한 할 경우, 물리학 분야인 전기자기학 중에서 쿨롱의 법칙으로부터 유도된 전기장의 개념을 도입하여 상권의 의미를 재해석할 수 있다. 향후 본 모델링에 대한 소비자 행동패턴의 실질적인 검증 및 추가 도입변수 및 에러항을 도입한 일반적인 모델링으로 발전시킬 수 있고, 그 또한 추가 연구주제가 될 수 있다.

• Journal of Korea Game Society
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• v.20 no.4
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• pp.3-10
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• 2020

The purpose of this paper is to make a game design to overcome traditional prevention system. Cases such as recent cockroach floodings in Seoul, which shows difficulties on understanding the appearance pattern of cockroaches and limitations of the quarantine systems are increasing frequently theses days. In particular public sewer systems which is essential for urban development, face problems due to urban sprawl. In order to solve the problem this study proposes a game design building collective intelligence through citizen participation on cockroach prevention problems.

• Journal of Microbiology and Biotechnology
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• v.24 no.7
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• pp.979-986
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• 2014

Plant pathogenic RNA viruses are present in a variety of plant-based foods. When ingested by humans, these viruses can survive the passage through the digestive tract, and are frequently detected in human feces. Kimchi is a traditional fermented Korean food made from cabbage or vegetables, with a variety of other plant-based ingredients, including ground red pepper and garlic paste. We analyzed microbial metatranscriptome data from kimchi at five fermentation stages to identify plant RNA virus-derived sequences. We successfully identified a substantial amount of plant RNA virus sequences, especially during the early stages of fermentation: 23.47% and 16.45% of total clean reads on days 7 and 13, respectively. The most abundant plant RNA virus sequences were from pepper mild mottle virus, a major pathogen of red peppers; this constituted 95% of the total RNA virus sequences identified throughout the fermentation period. We observed distinct sequencing read-depth distributions for plant RNA virus genomes, possibly implying intrinsic and/or technical biases during the metatranscriptome generation procedure. We also identified RNA virus sequences in publicly available microbial metatranscriptome data sets. We propose that metatranscriptome data may serve as a valuable resource for RNA virus detection, and a systematic screening of the ingredients may help prevent the use of virus-infected low-quality materials for food production.

• The Korean Journal of Physiology and Pharmacology
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• v.20 no.6
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• pp.595-603
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• 2016

Ribosomal S6 kinase is a family of serine/threonine protein kinases involved in the regulation of cell viability. There are two subfamilies of ribosomal s6 kinase, (p90rsk, p70rsk). Especially, p90rsk is known to be an important downstream kinase of p44/42 MAPK. We investigated the role of p90rsk on ethanol-induced cell proliferation of HepG2 cells. HepG2 cells were treated with 10~50 mM of ethanol with or without ERK and p90rsk inhibitors. Cell viability was measured by MTT assay. The expression of pERK1, NHE1 was measured by Western blots. The phosphorylation of p90rsk was measured by ELISA kits. The expression of Bcl-2 was measured by qRT-PCR. When the cells were treated with 10~30 mM of ethanol for 24 hour, it showed significant increase in cell viability versus control group. Besides, 10~30 mM of ethanol induced increased expression of pERK1, p-p90rsk, NHE1 and Bcl-2. Moreover treatment of p90rsk inhibitor attenuated the ethanol-induced increase in cell viability and NHE1 and Bcl-2 expression. In summary, these results suggest that p90rsk, a downstream kinase of ERK, plays a stimulatory role on ethanol-induced hepatocellular carcinoma progression by activating anti-apoptotic factor Bcl-2 and NHE1 known to regulate cell survival.