• The Korean Journal of Physiology and Pharmacology
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• v.26 no.5
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• pp.347-355
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• 2022

Abdominal aortic aneurysm (AAA) is a life-threatening disorder worldwide. Fibroblast growth factor 21 (FGF21) was shown to display a high level in the plasma of patients with AAA; however, its detailed functions underlying AAA pathogenesis are unclear. An in vitro AAA model was established in human aortic vascular smooth muscle cells (HASMCs) by angiotensin II (Ang-II) stimulation. Cell counting kit-8, wound healing, and Transwell assays were utilized for measuring cell proliferation and migration. RT-qPCR was used for detecting mRNA expression of FGF21 and activating transcription factor 4 (ATF4). Western blotting was utilized for assessing protein levels of FGF21, ATF4, and markers for the contractile phenotype of HASMCs. ChIP and luciferase reporter assays were implemented for identifying the binding relation between AFT4 and FGF21 promoters. FGF21 and ATF4 were both upregulated in Ang-II-treated HASMCs. Knocking down FGF21 attenuated Ang-II-induced proliferation, migration, and phenotype switch of HASMCs. ATF4 activated FGF21 transcription by binding to its promoter. FGF21 overexpression reversed AFT4 silencing-mediated inhibition of cell proliferation, migration, and phenotype switch. ATF4 transcriptionally upregulates FGF21 to promote the proliferation, migration, and phenotype switch of Ang-II-treated HASMCs.

• Journal of Ginseng Research
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• v.47 no.2
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• pp.237-245
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• 2023

Background: Ginsenoside Rg2 (Rg2) has a variety of pharmacological activities and provides benefits during inflammation, cancer, and other diseases. However, there are no reports about the relationship between Rg2 and atherosclerosis. Methods: We used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to detect the cell viability of Rg2 in vascular smooth muscle cells (VSMCs) and human umbilical vein endothelial cells (HUVECs). The expression of inflammatory factors in HUVECs and the expression of phenotypic transformation-related marker in VSMCs were detected at mRNA levels. Western blot method was used to detect the expression of inflammation pathways and the expression of phenotypic transformation at the protein levels. The rat carotid balloon injury model was performed to explore the effect of Rg2 on inflammation and phenotypic transformation in vivo. Results: Rg2 decreased the expression of inflammatory factors induced by lipopolysaccharide in HUVECs-without affecting cell viability. These events depend on the blocking regulation of NF-κB and p-ERK signaling pathway. In VSMCs, Rg2 can inhibit the proliferation, migration, and phenotypic transformation of VSMCs induced by platelet derived growth factor-BB (PDGF-BB)-which may contribute to its anti-atherosclerotic role. In rats with carotid balloon injury, Rg2 can reduce intimal proliferation after injury, regulate the inflammatory pathway to reduce inflammatory response, and also suppress the phenotypic transformation of VSMCs. Conclusion: These results suggest that Rg2 can exert its anti-atherosclerotic effect at the cellular level and animal level, which provides a more sufficient basis for ginseng as a functional dietary regulator.

• Journal of Microbiology and Biotechnology
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• v.33 no.2
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• pp.151-166
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• 2023

Yeast extract is a product prepared mainly from waste brewer's yeast, which is rich in nucleotides, proteins, amino acids, sugars and a variety of trace elements, and has the advantages of low production cost and abundant supply of raw material. Consequently, yeast extracts are widely used in various fields as animal feed additives, food flavoring agents and additives, cosmetic supplements, and microbial fermentation media; however, their full potential has not yet been realized. To improve understanding of current research knowledge, this review summarizes the ingredients, production technology, and applications of yeast extracts, and discusses the relationship between their properties and applications. Developmental trends and future prospects of yeast extract are also previewed, with the aim of providing a theoretical basis for the development and expansion of future applications.

• Structural Engineering and Mechanics
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• v.81 no.2
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• pp.205-217
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• 2022

The thermodynamic properties of shaft lining concrete (SLC) are important evidence for the design and construction, and the spatial variability of concrete materials can directly affect the stochastic thermal analysis of the concrete structures. In this work, an array of field experiments of the concrete materials are carried out, and the statistical characteristics of thermophysical parameters of SLC are obtained. The coefficient of variation (COV) and scale of fluctuation (SOF) of uncertain thermophysical parameters are estimated. A three-dimensional (3-D) stochastic thermal model of concrete materials with heat conduction and hydration heat is proposed, and the uncertain thermodynamic properties of SLC are computed by the self-compiled program. Model validation with the experimental and numerical temperatures is also presented. According to the relationship between autocorrelation functions distance (ACD) and SOF for the five theoretical autocorrelation functions (ACFs), the effects of the ACF, COV and ACD of concrete materials on the uncertain thermodynamic properties of SLC are analyzed. The results show that the spatial variability of concrete materials is subsistent. The average temperatures and standard deviation (SD) of inner SLC are the lowest while the outer SLC is the highest. The effects of five 3-D ACFs of concrete materials on uncertain thermodynamic properties of SLC are insignificant. The larger the COV of concrete materials is, the larger the SD of SLC will be. On the contrary, the longer the ACD of concrete materials is, the smaller the SD of SLC will be. The SD of temperature of SLC increases first and then decreases. This study can provide a reliable reference for the thermodynamic properties of SLC considering spatial variability of concrete materials.

• Geomechanics and Engineering
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• v.31 no.1
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• pp.1-14
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• 2022

Urban deep excavation will affect greatly on the deformation of adjacent existing buildings, especially those with shallow foundations. Isolation piles has been widely used in engineering to control the deformation of buildings adjacent to the excavation, but its applicability is still controversial. Based on a typical engineering, numerical calculation models were established and verified through monitoring data to study the influence characteristics of isolation piles on the deformation of existing shallow foundation buildings. Results reveal that adjacent buildings will increase building settlement δ_v and the deformation of diaphragm walls δ_h, while the isolation piles can effectively decrease these. The surface settlement curve is changed from "groove" type to "double groove" type. Sufficiently long isolation pile can effectively decrease δ_v, while short isolation piles will lead to a negative effect. When the building is within the range of the maximum settlement location P, maximum building rotation θ_m will increase with the pile length L and the relative position between isolation pile and building d/D increase (d is the distance between piles and diaphragm walls, D is the distance between buildings and diaphragm walls), instead, θ_m will decrease for buildings outside the location P, and the optimum was obtained when d/D=0.7.

• Steel and Composite Structures
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• v.49 no.4
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• pp.381-392
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• 2023

This study experimentally investigates the flexural behavior of steel-UHPC composite slabs composed of an innovative negative Poisson's ratio (NPR) steel plate and Ultra High Performance Concrete (UHPC) slab connected via demountable high-strength bolt shear connectors. Eight demountable composite slab specimens were fabricated and tested under traditional four-point bending method. The effects of loading histories (positive and negative bending moment), types of steel plate (NPR steel plate and Q355 steel plate) and spacings of high-strength bolts (150 mm, 200 mm and 250 mm) on the flexural behavior of demountable composite slab, including failure mode, load-deflection curve, interface relative slip, crack width and sectional strain distribution, were evaluated. The results revealed that under positive bending moment, the failure mode of composite slabs employing NPR steel plate was distinct from that with Q355 steel plate, which exhibited that part of high-strength bolts was cut off, part of pre-embedded padded extension nuts was pulled out, and UHPC collapsed due to instantaneous instability and etc. Besides, under the same spacing of high-strength bolts, NPR steel plate availably delayed and restrained the relative slip between steel plate and UHPC plate, thus significantly enhanced the cooperative deformation capacity, flexural stiffness and load capacity for composite slabs further. While under negative bending moment, NPR steel plate effectively improved the flexural capacity and deformation characteristics of composite slabs, but it has no obvious effect on the initial flexural stiffness of composite slabs. Meanwhile, the excellent crack-width control ability for UHPC endowed composite members with better durability. Furthermore, according to the sectional strain distribution analysis, due to the negative Poisson's ratio effect and high yield strength of NPR steel plate, the tensile strain between NPR steel plate and UHPC layer held strain compatibility during the whole loading process, and the magnitude of upward movement for sectional plastic neutral axis could be ignored with the increase of positive bending moment.

• Korean Journal of Radiology
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• v.20 no.6
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• pp.939-946
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• 2019

Objective: To report our initial experience of percutaneous sacroplasty (PSP) with an interpedicular approach for treating painful sacral metastases involving multiple sacral vertebral bodies. Materials and Methods: This study prospectively enrolled 10 consecutive patients (six men and four women; mean age, 56.3 ± 13.8 years) who underwent PSP for painful sacral metastases involving multiple sacral vertebral bodies from March 2017 to September 2018. Visual analogue scale (VAS) scores, Oswestry disability index (ODI) values, and the number of opioids prescribed to the patients were assessed before and after PSP. The procedure duration, length of hospitalization, and complications were also recorded. Results: Mean VAS and ODI declined significantly from 6.90 ± 1.20 and 74.40 ± 5.48 before the procedure to 2.70 ± 1.34 and 29.60 ± 14.57 after the procedure, respectively (p < 0.01). The median number of opioids prescribed per patient decreased from 2 (interquartile range [IQR] 1-3) pre-procedure to 1 (IQR 0-3) post-procedure (p < 0.01). Nine of the 10 patients showed no or decreased opioid usage, and only 1 patient showed unchanged usage. The mean procedure duration was 48.5 ± 3.0 minutes. The average length of hospitalization was 4.7 ± 1.7 days. Extraosseous cement leakage occurred in three cases without causing any clinical complications. Conclusion: PSP with an interpedicular approach is a safe and effective treatment in patients with painful sacral metastases involving multiple sacral vertebral bodies and can relieve pain and improve mobility.

• Structural Engineering and Mechanics
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• v.90 no.1
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• pp.27-40
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• 2024

The prediction of VIV amplitude is essential for the design and fatigue life estimation of steel tubes in tubular transmission towers. Limited to costly and time-consuming traditional experimental and computational fluid dynamics (CFD) methods, a machine learning (ML)-based method is proposed to efficiently predict the VIV amplitude of steel tubes in transmission towers. Firstly, by introducing the first-order mode shape to the two-dimensional CFD method, a simplified response analysis method (SRAM) is presented to calculate the VIV amplitude of steel tubes in transmission towers, which enables to build a dataset for training ML models. Then, by taking mass ratio M^*, damping ratio ξ, and reduced velocity U^* as the input variables, a Kriging-based prediction method (KPM) is further proposed to estimate the VIV amplitude of steel tubes in transmission towers by combining the SRAM with the Kriging-based ML model. Finally, the feasibility and effectiveness of the proposed methods are demonstrated by using three full-scale steel tubes with C-shaped, Cross-shaped, and Flange-plate joints, respectively. The results show that the SRAM can reasonably calculate the VIV amplitude, in which the relative errors of VIV maximum amplitude in three examples are less than 6%. Meanwhile, the KPM can well predict the VIV amplitude of steel tubes in transmission towers within the studied range of M^*, ξ and U^*. Particularly, the KPM presents an excellent capability in estimating the VIV maximum amplitude by using the reduced damping parameter S_G.

• Earthquakes and Structures
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• v.26 no.3
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• pp.175-189
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• 2024

Aiming at studying the plastic hinge (PH) evolution regularities and failure mode of rocking wall moment frame (RWMF) structure in earthquakes, the whole-working history analysis of seismic performance state of RWMF structure based on co-operation performance and PH evolution was carried out. Building upon the theoretical analysis of the elastic internal forces and deformations of RWMF structures, nonlinear finite element analysis (FEA) methods were employed to perform both Pushover analysis and seismic response time history analysis under different seismic coefficients (δ). The relationships among PH occurrence ratios (R_ph), inter-story drifts and δ were established. Based on the plotted curve of the seismic performance states, evaluation limits for the R_ph and inter-story drifts were provided for different performance states of RWMF structures. The results indicate that the R_ph of RWMF structures exhibits a nonlinear evolution trend of "fast at first, then slow" with the increasing of δ. The general pattern is characterized by the initial development of beam hinges in the middle stories, followed by the development towards the top and bottom stories until the beam hinges are fully formed. Subsequently, the development of column hinges shifts from the bottom and top stories towards the middle stories of the structure, ultimately leading to the loss of seismic lateral capacity with a failure mode of partial beam yield, demonstrating a global yielding pattern. Moreover, the limits for the R_ph and inter-story drifts effectively evaluate the five different performance states of RWMF structures.

• Geomechanics and Engineering
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• v.36 no.6
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• pp.601-618
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• 2024

The joint probability distribution of uncertain geomechanical parameters of geotechnical strata is a crucial aspect in constructing the reliability functional function for roof structures. However, due to the limited number of on-site exploration and test data samples, it is challenging to conduct a scientifically reliable analysis of roof geotechnical strata. This study proposes a Copula method based on small sample exploration and test data to construct the intensity characteristics of roof geotechnical strata. Firstly, the theory of multidimensional copula is systematically introduced, especially the construction of four-dimensional Gaussian copula. Secondly, data from measurements of 176 groups of geomechanical parameters of roof geotechnical strata in 31 coal mines in China are collected. The goodness of fit and simulation error of the four-dimensional Gaussian Copula constructed using the Pearson method, Kendall method, and Spearman methods are analyzed. Finally, the fitting effects of positive and negative correlation coefficients under different copula functions are discussed respectively. The results demonstrate that the established multidimensional Gaussian Copula joint distribution model can scientifically represent the uncertainty of geomechanical parameters in roof geotechnical strata. It provides an important theoretical basis for the study of reliability functional functions for roof structures. Different construction methods for multidimensional Gaussian Copula yield varying simulation effects. The Kendall method exhibits the best fit in constructing correlations of geotechnical parameters. For the bivariate Copula fitting ability of uncertain parameters in roof geotechnical strata, when the correlation is strong, Gaussian Copula demonstrates the best fit, and other Copula functions also show remarkable fitting ability in the region of fixed correlation parameters. The research results can offer valuable reference for the stability analysis of roof geotechnical engineering.