• Title/Summary/Keyword: Wa

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Prepartum Behaviors of Bos taurus coreanae (분만직전 한우의 행동)

  • Kim, Hyeon-Hui;Koo, Ja-Min;Hwang, Jae-Min;Jeon, Jung-Hwan;Chang, Hong-Hee;Lee, Won-Ik;Cheong, Jong-Tae;Lee, Hyo-Jong;Yeon, Seong-Chan
    • Korean Journal of Veterinary Research
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    • v.42 no.3
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    • pp.403-410
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    • 2002
  • This study was performed to investigate the general prepartum behavioral ethogram of Bos taurus coreanae (Hanwoo cow). In this study, 4 pregnant cows were placed in a separate area. We recorded the behaviors of the cows using time lapse VCR for 48 hours and analyzed behaviors with the scan point sampling method. We observed maintenance behaviors, social behaviors and ingestion behaviors. During the observation period, the time budgets of behaviors in order of frequency were LD(lying down, 38.2%), ST(standing, 24.7%), EA(eating, 10.7%), WA(walking, 7.2%), LR(lying down rumination, 5.6%), SR(standing rumination, 3.3%), TW(tail wagging, 3.1%) and SG(self grooming, 1.8%). The time budgets of the other behaviors such as PG(pairwise grooming), FC(fly catching) were negligible (<1%).

Antioxidative Effects of Solvent Fractions from Nandina domestica Fruits (남천(Nandina domestica) 열매 분획 추출물의 항산화 효과)

  • Seo, Soo-Jung;Shim, Kyu-Bong;Kim, Nam-Woo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.10
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    • pp.1371-1377
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    • 2011
  • This study was carried out to compare the antioxidative effects of various Nandina domestica fruits extracts. Organic fractions, including n-hexane, chloroform, ethyl acetate, and butanol fractions, were obtained from the water and ethanol extracts of N. domestica fruits. The phenolic compound content of the EE fraction was 922.22 mg/g. The flavonoid compound content was highest in the EB fraction (282.49 mg/g). The electron-donating ability was highest (93%) in the WA and EH fractions at 0.1 mg/mL. The SOD-like activity was showed the highest in the EA fraction (56.36%), and EH and EC both showed higher than 50% activity. The nitrite-scavenging ability of the EC fraction at 1.0 mg/mL under pH 1.2 conditions was 82.03%. The xanthine oxidase inhibitory activities of all the fractions, except WE, were higher than 90% at 0.5 mg/mL. The effect of tyrosinase inhibition was highest in the WE fraction (46.75%). These results show that the N. domestica fruits fractions contained high levels of polyphenol and flavonoid compounds, along with excellent antioxidative effects. This suggests that N. domestica fruits can be used as a functional material.

A Study on the Effects of Molding Pressure on the Compressive Strength and Durability of Soil-Cement Mixture (성형압력이 Soil-Cement의 강도 및 내구성에 미치는 영향에 관한 연구)

  • 서원명;고재군
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.20 no.1
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    • pp.4575-4591
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    • 1978
  • In order to investigate the effects of grain size distribution, cement content, and molding pressure on the strength and durability of soil-cement mixtures, a laboratory test of soil cement mixtures was performed at four levels of cement content, five levels of molding pressure, and four levels of normal curing periods. The results are summarized as follows: 1. Optimum moisture contents in loam soil and maximum dry density in sand soil increased with the increase of cement content, but in others, both optimum moisture contents and maximum dry density were changed ununiformly. 2. When the specimens were molded with molding pressure, 50kg/$\textrm{cm}^2$, strength of soil cement mixture with cement content, 2 and 4 per cent, was lower than the strength of soil cement mixture without cement content by more than 40 to 50 per cent. 3. The strength of soil-cement molded with molding pressure, 100kg/$\textrm{cm}^2$, was higher than the strength of soil-cement molded with M.D.D. obtained from standard compaction test more than 40 per cent in sand loam cement and 50 per cent in loamy cement. 4. There was highly significant positive correlation among molding pressure, cement content and unconfined compressive strentgh and so the following multiple regression equations were obtained. Loam: fc=1.9693C+0.197P-0.84 Sandy loam: fc=2.9065C+0.235P-0.77 5. When the specimens were molded with molding pressure, 20 to 100kg/$\textrm{cm}^2$, the regression equation between the 28-day and 7-day strenght was obtained as follows. Loam : q28=1.1050q7+7.59(r=0.9147) Sandy loam : q28=1.3905q7+3.17 (r=0.9801) 6. At the cement contents of above 50 per cent, the weight losses by freeeze-thaw test were negligible. At the cement content of below 8 per cent the weight losses were singnificantly high under low molding pressure and remarkably decreased with the increase of molding pressure up to 80kg/$\textrm{cm}^2$. 7. Resistance to damage from water and to absorption of water were not improved by molding pressure alone, but when the soil was mixtured with cement above 6 per cent, damage seldoms occurred and absorbed less than 5 per cent of water. 8. There was highly significant inverse-corelationship between the compressive strength of soil cement mixtures and their freeze-thaw loss as well as water absorption. By the regression equation methods, the relationships between them were expessed as followed fc=-7.3206Wa+115.6(r=0.9871) log fc=-0.0174L+1.59(r=0.7709) where fc=unconfined compressive stregth after 28-days curing. kg/$\textrm{cm}^2$ Wa=water absorption, % L : freeze-thaw loss rate, %

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Use of the Synthetic Gene Encoding the Truncated Human Rotavirus VP8* Protein in Escherichia coli for Production of Vaccine Candidates or Development of Diagnostic Antibodies (합성 유전자를 이용하여 Escherichia coli에서 백신 후보의 생산 혹은 진단용 항체의 개발을 위한 인간 rotavirus VP8* 부분 단백질의 발현)

  • Kim, Sang-Rae;Lee, Bheong-Uk
    • Journal of Life Science
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    • v.28 no.4
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    • pp.478-482
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    • 2018
  • Human rotavirus is a causative agent of acute diarrhea among children. The artificial gene encoding the truncated $VP8^*$ protein of human rotavirus A (serotype 1 strain WA) was synthesized according to the Escherichia coli codon preference. The synthetic $VP8^*$ gene also possessed the NdeI and HindIII restriction sites for the convenient in-frame cloning for translation and a 6-histidine tag at C-terminus for Ni+ affinity purification. Molecular weight of the truncated $VP8^*$ protein deduced from the nucleotide sequences of the artificial gene was a 19.7-kDa. This synthetic $VP8^*$ DNA fragment was inserted into the pT7-7 expression vector and transformed into E. coli BL21 (DE3). Transformants harboring the synthetic gene encoding the $VP8^*$ protein was induced by supplement of a final concentration of 0.05 mM ITPG at $20^{\circ}C$. Protein crude extract from the E. coli transformants was subjected to Western blotting with the mouse anti-rotavirus capsid antibody, showing ~20-kDa $VP8^*$ protein band. The truncated $VP8^*$ protein band was also observed by Western blotting using the rabbit polyclonal antibody serum made against the truncated $VP8^*$ protein. This study suggested that the synthetic gene could be used as an easy way to produce the antigenic vaccine candidate for control of virus-associated diseases or to develop antibodies for diagnostic purpose.

A Physiological Stimulating Factor of Water Intake during and after Dry Forage Feeding in Large-type Goats

  • Thang, Tran Van;Sunagawa, Katsunori;Nagamine, Itsuki;Kishi, Tetsuya;Ogura, Go
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.4
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    • pp.502-514
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    • 2012
  • When ruminants consume dry forage, they also drink large volumes of water. The objective of this study was to clarify which factor produced when feed boluses enter the rumen is mainly responsible for the marked increase in water intake in the second hour of the 2 h feeding period in large-type goats fed on dry forage for 2 h twice daily. Six large-type male esophageal- and ruminal-fistulated goats (crossbred Japanese Saanen/Nubian, aged 2 to 6 years, weighing $85.1{\pm}4.89kg$) were used in two experiments. In experiment 1, the water deprivation (WD) control and the water availability (WA) treatment were conducted to compare changes in water intake during and after dry forage feeding. In experiment 2, a normal feeding conditions (NFC) control and a feed bolus removal (FBR) treatment were carried out to investigate whether decrease in circulating plasma volume or increase in plasma osmolality is mainly responsible for the marked increase in water intake in the second hour of the 2 h feeding period. The results of experiment 1 showed that in the WA treatment, small amounts of water were consumed during the first hour of feeding while the majority of water intake was observed during the second hour of the 2 h feeding period. Therefore, the amounts of water consumed in the second hour of the 2 h feeding period accounted for 82.8% of the total water intake. The results of experiment 2 indicated that in comparison with the NFC control, decrease in plasma volume in the FBR treatment, which was indicated by increase in hematocrit and plasma total protein concentrations, was higher (p<0.05) in the second hour of the 2 h feeding period. However, plasma osmolality in the FBR treatment was lower (p<0.05) than compared to the NFC control from 30 min after the start of feeding. Therefore, thirst level in the FBR treatment was 82.7% less (p<0.01) compared with that in the NFC control upon conclusion of the 30 min drinking period. The results of the study indicate that the increased plasma osmolality in the second hour of the 2 h feeding period is the main physiological stimulating factor of water intake during and after dry forage feeding in large-type goats.

Effects of Heat Treatment of Chitooligosaccharide and Water-Soluble Chitosan on the Changes in Oligosaccharide Content and Anti-Rotaviral Activity (키토올리고당과 수용성 키토산의 열처리가 올리고당 함량의 변화와 항 로타바이러스성에 미치는 영향)

  • Park, B.S.;Kim, J.H.;Yu, D.H.;Yu, J.H.
    • Journal of Animal Science and Technology
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    • v.44 no.6
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    • pp.809-816
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    • 2002
  • Chitosan is the deacetylated product of chitin. Chitosan and its derivatives have many properties that make them attractive for a wide variety of health applications. This study was conducted to investigate change of oligosaccharide content and antiviral effect on rotavirus of chitooligosaccharide and water soluble chitosan after heat treatment. The quantitative analysis of oligosaccharide using colorimetry showed that oligosaccharide contents in water soluble chitosan and chitooligosaccharide were decreased from 62.67% to 60.45% and from 59.48% to 54.31%, respectively, after heating. The inhibitory effect of chitosan derivatives on MA-104 cell infected with human rotavirus(HRV) measured using AEC staining method. The inhibition level of 0.125% water-soluble chitosan against cell infection by human rotavirus was 91.98 3.09% in HRV S2 and was 89.92 1.68% in HRV Wa. But, chitooligosaccharide had not shown inhibitory effect against cell infection by HRV. It considered that most oligosaccharide of chitooligosaccharides consist of oligomer of lower polymerization degree. Heat treatment of water soluble chitosan and chitooligosaccharide did not influence their antiviral effects on rotavirus.

An Analysis of the Relationship between the Level of Elaboration Likelihood and the News Framing Effects (수용자의 인지정교화 가능성 수준이 프레이밍 효과에 미치는 영향에 관한 연구)

  • Jang, Ha-Yong;Je, Bang-Hoon
    • Korean journal of communication and information
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    • v.46
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    • pp.75-107
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    • 2009
  • Nevertheless reported the same events, news audience have diverse sense of sights and opinions about the events because of the different news frames. This notion was repeatedly evi nnced by several framing studies. This pa wa tried to analyse an interacting relationship between viewer’s level of elaboration likelihood and the effects of the news frames. This pa wa sfrrted with a discussion about the framing effects, then compared them with Elaboration Likelihood Ms notraming effely. And this study conducted an ex waiment selecting indivi ual dispngitions (involvement and cognitive complexity) and message characteristics(number of cues and arguments) as intermediating variables on the message framing effects. This study found out that, the more involvement about the issues the viewers had, the more their thoughts coincided with the issue's frame. On the other hand, when the viewers had low involvement about the issues and cognitive complexity, the framing effects were not found because they processed the messages through the peripheral route. Although the viewers' cognitive complexity was a factor in choosing the central route, but it was not directly connected to the framing effect. Both the number of cues and argument diversity in the messages had positive relationships with the framing effects.

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Elastic Wave Modeling Including Surface Topography Using a Weighted-Averaging Finite Element Method in Frequency Domain (지형을 고려한 주파수 영역 가중평균 유한요소법 탄성파 모델링)

  • Choi, Ji-Hyang;Nam, Myung-Jin;Min, Dong-Joo;Shin, Chang-Soo;Suh, Jung-Hee
    • Geophysics and Geophysical Exploration
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    • v.11 no.2
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    • pp.93-98
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    • 2008
  • Abstract: Surface topography has a significant influence on seismic wave propagation in a reflection seismic exploration. Effects of surface topography on two-dimensional elastic wave propagation are investigated through modeling using a weighted-averaging (WA) finite-element method (FEM), which is computationally more efficient than conventional FEM. Effects of air layer on wave propagation are also investigated using flat surface models with and without air. To validate our scheme in modeling including topography, we compare WA FEM results for irregular topographic models against those derived from conventional FEM using one set of rectangular elements. For the irregular surface topography models, elastic wave propagation is simulated to show that breaks in slope act as a new source for diffracted waves, and that Rayleigh waves are more seriously distorted by surface topography than P-waves.

The Effect of Roofing Materials for Using Harvested Rainwater (집수빗물이용을 위한 지붕면 재질의 영향)

  • Lee, Ju Young;Kim, Tschung-Il;Kim, Hyunwoo;Han, Mooyoung
    • Journal of Korean Society of Environmental Engineers
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    • v.34 no.9
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    • pp.623-629
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    • 2012
  • The objective was to evaluate the quality of harvested rainwater of the roofing materials used and the presence of lichens/mosses on the roofing surface. This experiment was studied in the field where five pilot structures with different roofing materials (i.e., wooden shingle tiles, wooden shingle tiles (including mosses), concrete tiles, clay tiles [Gi-Wa] and slate tiles) were installed. As a result, the clay tiles [Gi-Wa] was found to be the most suitable for rainwater harvesting applications. It was due to the disinfection from ultraviolet light and high temperature according to characteristic of roofing materials. Comparison with quality of rainwater on that wooden shingle tiles with and without including mosses, which may affect the concentration of and wooden shingle tiles including mosses, mosses may affect concentration of TOC, nitrate, and sulfate. Additionally, the average concentration of asbestos was determined to be measured at $0.002/cm^3$ for the slate tiles. The value satisfied with it met the Ministry of Environment's Multi-use facility standard and Ministry of Labor's Office standard ($0.01/cm^3$).

Ultrasound Imaging for Position of the Sciatic Nerve Division in Korean Female (한국 여성에서 좌골 신경 분지 위치에 대한 초음파 영상)

  • Nam, Il Hyun;Yeo, Eui Dong;Yu, Ji Soo;Lee, Jun Ho;Lee, Young Koo
    • The Journal of Korean Orthopaedic Ultrasound Society
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    • v.7 no.1
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    • pp.28-32
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    • 2014
  • Purpose: We aim to determine sciatic nerve bifurcation location and depth at the level of the popliteal fossa in Korean female. Materials and Methods: Thirty three subjects were enrolled in Korean female. M-turbo ultrasound system (SonoSite, bothell, WA, USA)with 38 mm high frequency linear array transducer, 13-6 MHz probe was used for ultrasound measurements. With subject lying prone, the location of the sciatic nerve in relation to the popliteal crease and skin to nerve distance were assessed via ultrasound. Analyses were performed with SAS version 9.3 using multiple linear regression. Results: Thirty three subjects were enrolled. Distance from the popliteal crease to the sciatic nerve was 4.5-7.5 (mean 5.7 cm), and the depth of the sciatic nerve from the skin was 1.8-3.2 (mean 2.4 cm). Multiple linear regression for the usefulness of the model has a p value of 0.036, shows between weight and depth. Conclusion: We show that variability exists for sciatic nerve bifurcation location in Korean female, The success rate is creased if consider the relations between weight and depth when performs sciatic nerve block in Korean female. In our study, a sciatic nerve block is recommend that performed 7.5 cm proximal to the crease in the popliteal fossa.

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