• BMB Reports
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• 제51권8호
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• pp.412-417
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• 2018

Homotypic cell-in-cell (CIC) structures forming between cancer cells were proposed to promote tumor evolution via entosis, a nonapoptotic cell death process. However, the mechanisms underlying their formation remained poorly understood. We performed a microarray analysis to identify genes associated with homotypic CIC formation. Cancer cells differing in their ability to form homotypic CIC structures were selected for the study. Association analysis identified 73 probe sets for 62 candidate genes potentially involved in CIC formation. Among them, twenty-one genes were downregulated while 41 genes were upregulated. Pathway analysis identified a gene interaction network centered on IL-8, which was upregulated in high CIC cells. Remarkably, CIC formation was significantly inhibited by IL-8 knockdown and enhanced upon recombinant IL-8 treatment, which correlated with altered cell-cell adhesion and expression of adhesive molecules such as P-cadherin and ${\gamma}$-catenin. Together, our work identified IL-8 as a positive regulator of homotypic CIC formation via enhancing intercellular adhesion.

• Journal of Biosystems Engineering
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• 제29권2호
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• pp.151-158
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• 2004

This research was conducted to study the offensive odor adhesion efficiency of filter bed materials using the experimental column that was designed and constructed in this work. The offensive odor adhesion experiment was conducted using mixture of high physical adhesion efficiency material, and the fixity of deodorization microorganism of selected filter bed material was tested using ammonia exclude microorganism A4-2 and sulfur oxidation microorganism S5-5.2 those were cultured at the Agricultural Chemical Department of Chungnam National University, and deodorization efficiency of selected filter bed material mixture was tested. Followings are summary of these tests results. 1) Amount of elimination of the offensive odor gas ammonia and hydrogen sulfide per unit volume were 0.054 and 0.016 $\ell$/㎤ in rice hull, 0.01 and 0.004 $\ell$/㎤ in rice straw, 0.158 and 0.01 $\ell$/㎤ in coconut, 0.014 and 0.02 $\ell$/㎤ in perlite, 0.004 and 0.003 $\ell$/㎤ in high road ball, and 0.112 and 0.015 $\ell$/㎤ chaff of pine, respectively. 2) Amount of elimination of offensive odor gas of ammonia and hydrogen sulfide per unit vloume were 0.079 and 0.016 $\ell$/㎤ in mixture 1, 0.045 and 0.014 $\ell$/㎤ in mixture 2, 0.123 and 0.017 $\ell$/㎤ in mixture 3, 0.055 and 0.016 $\ell$/㎤ in mixture 4, 0.031 and 0.015 $\ell$/㎤ in mixture 5, and 0.111 and 0.020 $\ell$/㎤ in mixture 6, respectively. 3) The offensive odor elimination microoraganism inoculated to the mixture of chaff of pine (70%) and pert (30%) showed the elimination efficiency of 99.06% and 96.61% against the ammonia and hydrongen sulfide, respectively, during 24 hours period.

• 접착 및 계면
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• 제17권3호
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• pp.96-103
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• 2016

본 연구에서는 셀룰로스계 천연섬유인 대나무와 poly(lactic acid) (PLA)로 구성된 펠렛을 압출공정으로 제조하고, 여러 가지 함량의 대나무섬유/PLA 바이오복합재료를 사출공정을 통해 성형하였다. 바이오복합재료의 굴곡, 인장, 충격 특성 및 열변형온도에 미치는 대나무섬유의 수처리 영향을 조사하였다. 천연섬유의 열안정성, 바이오복합재료의 굴곡특성, 인장탄성률 및 충격특성은 섬유 함량은 물론 수처리 유 무에 의존한 반면, 열변형온도는 주로 수처리에 의해 영향을 받았다. 바이오복합재료의 기계적 특성과 충격특성의 증가는 이를 구성하고 있는 대나무섬유와 PLA 매트릭스 사이의 계면결합력이 대나무섬유의 수처리에 의해 향상되었기 때문이다. 연구결과는 친환경적이고 작업친화적인 물을 이용한 천연섬유의 전처리가 바이오복합재료의 성능을 향상시키는데 기여할 수 있다는 것을 제시하여 준다.

• Journal of Periodontal and Implant Science
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• 제48권1호
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• pp.12-21
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• 2018

Purpose: The goal of this study was to develop and validate a standardized in vitro pathogenic biofilm attached onto saliva-coated surfaces. Methods: Fusobacterium nucleatum (F. nucleatum) and Porphyromonas gingivalis (P. gingivalis) strains were grown under anaerobic conditions as single species and in dual-species cultures. Initially, the bacterial biomass was evaluated at 24 and 48 hours to determine the optimal timing for the adhesion phase onto saliva-coated polystyrene surfaces. Thereafter, biofilm development was assessed over time by crystal violet staining and scanning electron microscopy. Results: The data showed no significant difference in the overall biomass after 48 hours for P. gingivalis in single- and dual-species conditions. After adhesion, P. gingivalis in single- and dual-species biofilms accumulated a substantially higher biomass after 7 days of incubation than after 3 days, but no significant difference was found between 5 and 7 days. Although the biomass of the F. nucleatum biofilm was higher at 3 days, no difference was found at 3, 5, or 7 days of incubation. Conclusions: Polystyrene substrates from well plates work as a standard surface and provide reproducible results for in vitro biofilm models. Our biofilm model could serve as a reference point for studies investigating biofilms on different surfaces.

• Elastomers and Composites
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• 제1권1호
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• pp.49-55
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• 1966

Based upon the results of the previous work, the experiments are concentrated on the improvement of the durability of rubber coated fabrics as a rain garment material. To obtain a much lighter and durable coated fabric, several kinds of light weight cotton prints having almost equal density in warp and fillingwise were chosen. Rubber coating on these prints was made by topping and spreading process using rubber compound of various viscosity, and the physical properties of final product were analysed and interpreted in terms of adhesion and durability. The results are as follows. 1. Any noticeable difference between two coating processes was not found in terms of physical properties. 2. Base fabrics should be dipped once into a dilute rubber compound before coating operation in order to obtain a uniform adhesion and physical properties, and the optimum range of the viscosity of dipping paste are from 100 poise to 200 poise. 3. Generally, the tearing strength of the coated fabrics is inversely proportional to the adhesion. 4. It was assumed that the increase of the water proofness after water immersion on the finished material which have dense base fabric is chiefly due to the swelling of the cellulosic fiber.

• 접착 및 계면
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• 제6권4호
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• pp.1-6
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• 2005

본 실험에서는 화학적 표면처리된 탄화규소의 첨가가 탄화규소(SiC)/에폭시 복합재료의 critical strain energy release rate mode II ($G_{IIC}$) 특성에 미치는 영향에 대하여 알아보았으며, 표면처리된 SiC의 표면특성은 산 염기도와 FT-IR을 사용하여 알아보았다. 또한 복합재료의 기계적 계면물성은 $G_{IIC}$를 통하여 알아보았다. 실험결과, 산성 용액으로 표면처리한 SiC (A-SiC)의 표면 산도가 염기성(B-SiC) 또는 표면처리 하지 않은 SiC (V-SiC)보다 높으며, $G_{IIC}$의 크랙저항 특성은 A-SiC가 향상되었는데, 이러한 결과는 SiC 충전재와 에폭시 수지간의 분자간 계면결합력의 향상 때문으로 판단된다.

• Advances in concrete construction
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• 제13권4호
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• pp.315-325
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• 2022

Reinforced concrete structures are exposed throughout their lifetime to the phenomenon of carbonation, which considerably influences their durability by causing corrosion of the reinforcements. The fight against this phenomenon is usually ensured by anti-carbonation coatings which have the possibility of limiting the permeability to carbon dioxide or with coatings which absorb the CO₂ present in the air. A coating with good crack-bridging (sealing) capacity will prevent water from entering through existing cracks in concrete. Despite the beneficial effect of these coatings, their durability decreases considerably over time with temperature and humidity. In order to use coatings made from local materials, not presenting any danger, available in abundance in our country, very economical and easy to operate is the main objective of this work. This paper aim is to contribute to the formulation of a corrected dune sand-based mortar as an anti-carbonation coating for concrete. The results obtained show that the cement mortar based on dune sand formulated has a very satisfactory compressive strength, a very low water porosity compared to ordinary cement mortar and that this mortar allows an improvement in the protection of the concrete against the carbonation of 60% compared to ordinary cement mortar based on alluvial sand. Moreover, the formulated cement mortars based on dune sand have good adhesion to the concrete support, their adhesion strengths are greater than 1.5MPa recommended by the standards.

• 한국진공학회지
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• 제20권5호
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• pp.333-338
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• 2011

본 연구에서는 전자석 코일 마그네트론 소스를 가지는 비대칭 마그네트론 스퍼터링 장치를 이용하여 보호 코팅 소재로 사용되어지는 비정질 탄소박막을 제작하였다. 내부 전자석 코일의 전류를 고정하고 외부 전자석 코일의 전류를 다양하게 변화시켜 탄소 박막을 제작하였고, 제작되어진 박막들의 경도, 마찰계수, 접착력, 표면 거칠기 등의 트라이볼로지 특성들을 측정하였고, 라만과 HRTEM을 이용하여 구조적 특성을 평가하였으며, 이들 상호간에 관계를 규명하였다. 결과로서, 제작되어진 탄소박막의 경도, 마찰계수, 접착 특성은 외부 전자석 코일 전류가 증가함에 따라 향상되었으며, 이러한 결과는 박막내에 결합력이 강한 $sp^2$ 결합과 클러스터의 형성과 관련된다. 전자석 코일 전류의 증가는 전자와 이온 밀도의 증가시키고, 기판에서 이온의 충돌의 증가와 기판온도 향상을 야기한다. 이러한 현상의 박막내에 증가되어진 $sp^2$ 결합과 클러스터들의 형성은 탄소박막의 트라이볼로지 특성 향상에 기여하였다.

• Composites Research
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• 제16권3호
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• pp.41-48
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• 2003

본 연구에서는 고온 산화분위기 하에서 탄소/탄소 복합재료의 내산화 특성 향상을 위해 사용된 이규화 몰리브덴(MoSi$_2$)의 함량(0. 4, 12. 그리고 20 wt%)에 따른 복합재료의 충격특성에 관하여 고찰하였다. 결과로서, MoSi$_2$가 함침된 탄소/탄소 복합재료는 이를 함유하지 않은 것에 비하여 충격특성 향상의 원인이 되는 탄소섬유와 매트릭스 수지간의 계면결합력 증가를 가져왔으며, 특히 12 wt% MoSi$_2$가 현재의 시스템에서 가장 우수한 충격특성을 나타내었다. 이는 복합재료 내의 탄소섬유, 필러, 그리고 매트릭스 수지간의 계면 접착력의 증가를 가져오는 90$0^{\circ}C$ 전후에서 MoSi$_2$의 취성­연성 전이(BDT) 작용 때문이라 사료된다.

• 접착 및 계면
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• 제15권1호
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• pp.9-13
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• 2014

본 연구는 폴리프로필렌/마이카 복합재료의 고상압출에 의한 배향에 따른 기계적 물성의 변화에 관한 연구이다. 마이카 함량이 증가할수록 복합재료의 비중이 증가하였다. 하지만 고상압출에 따른 배향에 의해 복합재료의 비중은 미세공극의 발생으로 말미암아 비중은 배향 전에 비해 현저히 감소하는 것으로 나타났다. 이러한 미세공극의 존재는 복합재료의 인장 및 굴곡물성에 큰 영향을 미치는 것으로 나타났다. 고상압출 여부에 관계없이 마이카 함량이 10 wt%일 때 최대 굴곡 물성이 관찰되었다.