• 대한임상검사과학회지
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• 제45권1호
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• pp.37-42
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• 2013

The cells are concentrated approximately 20-fold by cytocentrifugation. This study evaluated the nucleated cell number for cells recovered on slide by using Cytospin-3 (Thermo Shandon Ltd. UK) and Cytopro-7620 (Wescor Inc., USA) cytocentrifuges to hematocytometer cell count of $0{\sim}5WBCs/{\mu}L$ of hematocytometer in the cerebrospinal fluid cell count. One hundred forty eight samples of $0{\sim}5WBCs/{\mu}L$ on hematocytometer, were cytocentrifuged by Cytospin-3 and Cytopro-7620 instruments. The nucleated cell number for cells recovered on slide was counted after Wright stain. The nucleated cell number for cells recovered on slide was 0~40 cells in the 44 samples of $0WBC/{\mu}L$, and 3~95 cells in the 31 samples of $1WBC/{\mu}L$. It was observed that the nucleated cell number for cells recovered on slide was 13~100 cells in the 44 samples of $2WBCs/{\mu}L$, and more than 100 cells in the 29 samples of $3{\sim}5WBCs/{\mu}L$, respectively. In addition, extremely normal lymphocyte, monocyte and polymorphonuclear neutrophil were observed in the 143 samples of $0{\sim}5WBCs/{\mu}L$. Macrophage and eosinophil were also rarely observed. The nucleated cell number for cells recovered on slide was 20 cells, which were regarded as $1WBC/{\mu}L$ in body fluid cell count. However, in this study, we made alterations to report nucleated cell percentage as 0% without preparing the cytocentrifuged slide at $0WBC/{\mu}L$ by using the cell yield in a comparison between the value of $0{\sim}5WBCs/{\mu}L$ and nucleated cell number for cells recovered on slide.

• 대한임상검사과학회지
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• 제55권2호
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• pp.82-92
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• 2023

소변 침전물은 일반적으로 검사자가 원심분리한 소변을 현미경으로 검사한다. 따라서 소변 침전물 표본제작 과정의 차이가 검사 결과에 영향을 미치는지 확인했다. 요시험지 검사와 cobas u 701 analyzer에서 각각 백혈구 및 적혈구가 양성 반응으로 나온 107개의 신선한 검체를 수동 현미경검사를 위해 선택했다. 연구에서는 백혈구와 적혈구에 대해 자동분석기와 세 가지 수동 현미경검사법을 각각의 검사지침에 따라 평가하였다. 백혈구와 적혈구에 대한 cobas u 701 analyzer와 대한임상검사정도관리협회 간의 결정계수는 각각 r²=0.977과 r²=0.970이었다. 백혈구와 적혈구에 대한 cobas u 701 analyzer와 대한 임상검사정도관리협회 간의 일치도는 각각 74.8%, 77.6%였다. 대한임상검사정도관리협회 방법으로 표본을 제작하여 검사한 결과에서 자동분석기와 가장 좋은 상관성 및 일치도를 보였다. 결과적으로 소변 침전물 표본제작 방법의 차이는 침전물 농도에 영향을 미쳤으며 이는 HPF당 세포 등급과 수에 영향을 미쳤다. 따라서 보다 일관되고 정확한 검사 결과를 위해 소변 침전물 표본제작 과정을 표준화하는 것이 좋을 것으로 사료된다.

• International Journal of Computer Science & Network Security
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• 제21권9호
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• pp.223-229
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• 2021

Classification of different blood cell types is an essential task for human's medical treatment. The white blood cells have different types of cells. Counting total White Blood Cells (WBC) and differential of the WBC types are required by the physicians to diagnose the disease correctly. This paper used transfer learning methods to the pre-trained deep learning models to classify different WBCs. The best pre-trained model was Inception ResNetV2 with Adam optimizer that produced classification accuracy of 98.4% for the dataset comprising four types of WBCs.

• Molecules and Cells
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• 제22권2호
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• pp.189-197
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• 2006

Prolactin (PRL) is a pituitary hormone involved in various physiological processes, including lactation, mammary development, and immune function. To further investigate the in vivo and comparative endocrine roles of PRL, mouse PRL cDNA fused to the cytomegalovirus promoter, was introduced into muscle by direct injection. Previously we studied the function of rat PRL using the same protocol. PRL mRNA was detected in the muscle following injection by RT-PCR and subsequent Southern blot analysis. PRL was also detected and Western blot analysis revealed a relatively high level of serum PRL. In the pCMV-mPRL-injected female mice, the estrous cycle was extended, especially in diestrus stage and the uterus thickening that was shown in normal estrous stage was not observed. In the pCMV-mPRL-injected male mice, new blood vessels were first found at 5 weeks of age and fully developed blood vessels were found after 8 weeks in the testis. The number of Leydig cells increased within the testis and the testosterone level in serum was observed high. Finally, the number of white blood cells (WBCs) increased in the pCMV-mPRL-injected mice. The augmentation of WBCs persisted for at least 20 days after injection. When injection was combined with adrenalectomy, there was an even greater increase in number of WBCs, especially lymphocytes. This increase was returned normal by treatment with dexamethansone. Taken together, our data reveal that intramuscularly expressed mouse PRL influences reproductive functions in female, induces formation of new blood vessels in the testis, and augments WBC numbers. Of notice is that the Leydig cell proliferation with increased testosterone was conspicuously observed in the pCMV-mPRL-injected mice. These results also suggest subtle difference in function of PRL between mouse and rat species.

• Journal of Microbiology and Biotechnology
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• 제25권10호
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• pp.1728-1733
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• 2015

This study was done to establish a mouse model for catheter-related biofilm infection suitable to bioluminescence imaging (BLI). Biofilm formation of Pseudomonas aeruginosa (P. aeruginosa) Xen5 grown on catheter disks in vitro and in an implanted mouse model was real-time monitored during a 7-day study period using BLI. The numbers of integrated brightness (IB) and viable bacterial count (VBC) in the biofilm disks in vitro were highest at 24 h after inoculation; the IB of biofilm in vivo was increased until 24 h after implantation. A statistical correlation was observed between IB and VBC in vitro by linear regression analysis. The actual VBC value in vivo can be estimated accurately by IB without sacrifice. In addition, we monitored the change in white blood cells (WBCs) during infection. The number of WBCs on day 7 was significantly higher in the infection group than in the control group. This study indicates that BLI is a simple, fast, and sensitive method to measure catheter biofilm infection in mice.

• 한방안이비인후피부과학회지
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• 제28권1호
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• pp.68-84
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• 2015

Object : The object of this study was effected of composed of 4 herbal medicines(Rubus coreanus, Rehmanniae Radix, Houttuynia cordata, Betulae cortex) on acute atopic dermatitis mice. Methods : BALB/c mice were divided 4 groups ; normal group, negative control group of acute atopic dermatitis mice induced by DNCB, treated apply to the back skin with the herbal medicine group, and treated orally with herbal medicine group. Treated with herbal medicine and DNCB during 2 weeks. After treated, measured WBCs, RBCs, and platelet in the blood, and analysed mRNA expression of spleen. Result : Composed herbal medicines could reduce WBCs, lymphocytes, monocytes, neutrophils, and eosinophils in the mouse blood. And, could down TNF-${\alpha}$ levels in spleen. In the skin tissue histology results, herbal medicines reduce the cells and T cells. Conclusion : Composed herbal medicines help to another inflammatory disease because they reduce the cells and T cells in the skin tissues. And they have inhibitory effects of TNF-${\alpha}$ mRNA expression, and Th 2 immune responses. Therefore herbal medicines use for an acute atopic dermatitis treatment.

• 여성건강간호학회지
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• 제11권4호
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• pp.265-272
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• 2005

Purpose: To investigate the effect of infant massage on stress, immune function and self-confidence in postpartum mothers. Method: From September, 2003 to February, 2004, 65 women who had delivered babies and were enrolled in the postpartum clinic were assigned to the experimental group or control group. In the experimental group, infant massage was given for 20 minute sessions twice a day from 4 days to 14 days after delivery. The instruments were Parental Stress Index, serum cortisol levels, WBCs, lymphocytes, and Self Confidence Scale. Results: Stress scores, WBCs and lymphocytes showed significant differences by the time, but there weren't significant differences according to the group and interactions between the group and the time. Cortisol and self-confidence scores showed significant differences by the time and the group. Conclusions: Applying massage to their newborn baby influenced cortisol levels of postpartum mothers. Therefore stress, cortisol, and immune functions in postpartum mothers should be generally screened and follow up studies are needed for the effective application of massage in postpartum mothers.

• 한국컴퓨터정보학회논문지
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• 제23권4호
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• pp.147-153
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• 2018

In this paper, we propose an efficient WBC 14-Diff classification which performs using the WBC-ResNet-152, a type of CNN model. The main point of view is to use Super-pixel for the segmentation of the image of WBC, and to use ResNet for the classification of WBC. A total of 136,164 blood image samples (224x224) were grouped for image segmentation, training, training verification, and final test performance analysis. Image segmentation using super-pixels have different number of images for each classes, so weighted average was applied and therefore image segmentation error was low at 7.23%. Using the training data-set for training 50 times, and using soft-max classifier, TPR average of 80.3% for the training set of 8,827 images was achieved. Based on this, using verification data-set of 21,437 images, 14-Diff classification TPR average of normal WBCs were at 93.4% and TPR average of abnormal WBCs were at 83.3%. The result and methodology of this research demonstrates the usefulness of artificial intelligence technology in the blood cell image classification field. WBC-ResNet-152 based morphology approach is shown to be meaningful and worthwhile method. And based on stored medical data, in-depth diagnosis and early detection of curable diseases is expected to improve the quality of treatment.

• 한국세라믹학회지
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• 제38권11호
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• pp.1067-1074
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• 2001

수팽윤성 점토로서 벤토나이트(BEN), 유기금속 비누계 건조제, 아크릴계 바인더 및 코팅첨가제들을 배합하여 서로 성분이 다른 자동산화 건조형 수성코팅제(WBC-1, WBC-2, WBC-3, WBC-4) 4종류를 제조하였다. 제조한 수성코팅제(WBC)의 용액점도, 고형분, 유동학적인 성질 및 자동산화 건조성을 조사하였다. 또한 주조된 WBC 필름의 열안정성, 투명성 및 내수성을 측정하였고, WBC 필름의 표면형상을 주사탐침현미경으로 조사하였다. BEN이 포함된 WBC-2, WBC-3 및 WBC-4는 전단력에 따라 요변성이 나타남으로서 WBC의 저장안정성이 우수하였다. 자동산화형 WBC의 건조성은 건조제가 Mn/Zn/Ba=1/2/3의 비로 혼합되었을 때, 6$0^{\circ}C$에서 5초로서 최대치를 나타내었다. 또한 BEN이 포함된 WBC 필름의 초기분해온도와 투명성은 시판 WBC(MC-21W)의 필름보다 32.2~54.7$^{\circ}C$와 5.1~8.6%의 범위로 증가하였고, WBC 필름의 내수성은 MC-21W

• Asian Pacific Journal of Cancer Prevention
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• 제16권6호
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• pp.2303-2306
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• 2015

Objective: Skin tumors are the most commonly seen cancer type worldwide. Regarding pathogenesis, it is thought that disruption of kinetics through T lymphocyte-mediated development of chronic inflammation may be involved. The present study was intended to identify role of inflammatory cells such as neutrophils, monocytes and lymphocytes in the determination of risk for skin cancer. Materials and Methods: We retrospectively reviewed charts of 569 cases diagnosed as having primary skin tumors. Data regarding age, gender and histopathological subtype were recorded. Blood parameters studied on the day before surgery including WBCs, neutrophils, and lymphocyte counts, neutrophil:lymphocyte and neutrophil:monocyte ratios were also recorded. Two-hundred and two healthy individuals presented for check-up in an outpatient clinic were selected as the control group. Parameters studied in cases with skin cancer were compared to those healthy individuals. Findings: Of the cases with skin cancer, 401 were basal cell carcinoma (BCC) while 144 were squamous cell carcinoma (SCC) and 13 were malignant melanoma (MM). WBC, neutrophil and monocyte counts and the neutrophil:lymphocyte ratio were found to be lower in the patient group than in the healthy control group (p<0.001) while no significant difference was found in other parameters reviewed (p>0.05). No significant difference was found in WBC, neutrophil, neutrophil: monocyte ratio according to gender (p>0.05). Monocyte count was found to be $0.68{\pm}0.61$ in men and $0.55{\pm}0.25$ in women, indicating strong statistical significance (p<0.001). WBC, neutrophil and monocyte values were highest in control group while lowest in BCC. When BCC and SCC groups were compared to controls, significant differences found (p<0.001). There were no significant differences in lymphocyte counts among groups (p=0.976). Neutrophil:lymphocyte ratios were 3.24 in BCC, 3.59 in SCC, 3.44 in MM and 5.06 in control group (p<0.001). Conclusions: In our study, it was found that there were significant differences in complete blood count, neutrophil, monocyte and neutrophil:lymphocyte levels among groups. Neutrophil: lymphocyte ratio was found to be lowest in BCC among skin cancers.