• Title/Summary/Keyword: W-substitution

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The Utilization of Rumen Content-Barley Meal in Diets of Growing Lambs

  • Abouhief, M.A.;Kraidees, M.S.;Al-Selbood, B.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.8
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    • pp.1234-1240
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    • 1999
  • The nutritive value of rumen contents and barley mixture (4:1 w/w; RCB) was evaluated and the effect of their feeding on growth performances in Najdi lambs was studied. A metabolism trial was conducted with 16 rams divided into four dietary groups. The diets were: a whole-mixed control diet and three diets where RCB was incorporated at the rates of 25, 50 and 100%, replacing an equal amount of control diet. The results showed that there was a depression (p<0.05) in DM digestibility for the 100% RCB diet in comparison with other diets. The digestibility of CP was higher(p<0.05) for the 25% RCB diet as compared to the control diet; there was a trend for a small (p>0.05) decrease in digestibility as level of RCB increased. Lambs in all studied diets were in positive nitrogen balance; the differences between diets were not significant. A total of 45 lambs were allotted into three groups and used to evaluate the effect of dietary inclusion of RCB (0, 25 and 50%) on growth performance and carcass traits. Daily DM intake, final body weight, carcass weight and dressing percentage were not different among treatments. Average weight gain and ether extract (EE) in 9-11th control joint were higher (p<0.05) in lambs fed control diet than those fed RCB diets. The substitution of RCB for 50% of control diet exhibited 11.8% reduction in feeding cost for each kg of body weight gain.

Improvement of Production and Secretion of Heterologous \alpha-Amylase from Saccharomyces cerevisiae. (외래 알파아밀라제의 Saccharomyces cerevisiae에서의 생산과 분비효율의 증진)

  • Choi, Sung-Ho;Kim, Geun
    • Microbiology and Biotechnology Letters
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    • v.31 no.1
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    • pp.36-41
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    • 2003
  • In order to increase the production and secretion rate of mouse salivary $\alpha$-amylase from Saccharomyces cerevisiae, various experiments were attempted. A plasmid pCNNinv (AMY) was constructed by the substitution of ADCl promoter and native signal sequence of mouse salivary $\alpha$-amylase cDNA gene with PRBI promoter and yeast invertase leader sequence, which resulted in 25% increase in the production of $\alpha$-amylase in the culture medium. The respiratory deficient transformant carrying pCNNinv (AMY) were obtained by treating yeast cells with ethidium bromide, and the $\alpha$-amylase activities in the culture brothes of the respiratory-deficient transformants were 5-8 times higher than that of parental wild type strain. $\alpha$-Amylase activity was also increased 3 times when the 0.015% (w/v) of 2-mercaptoethanol was added to the culture medium.

The Development of Rotoless Electronic Circulating Pump System with Internal Three Way Valve for Boiler - Objective for the 1st Year : Development of New Housing with Internal Three Way Valve - (보일러용 3way valve내장형 rotoless 전자식순환펌프 시스템의 개발 - 1차년도 목표 : 3way valve 내장형 하우징의 개발 -)

  • Han, J.W.;Kum, S.M.;Ryu, B.H.;Lee, C.E.;Ohu, S.C.;Yim, J.S.
    • Solar Energy
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    • v.16 no.2
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    • pp.97-112
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    • 1996
  • The purpose of research is to complement the circulating pump of gas boiler for the domestic and to develop a pump housing with internal three way valve: Housing and three way valve have been apart in the existing pump system. Based on the experimental result on can-typed circulating pump with existing housing, a new housing with internal three way valve was designed and manufactured. The performance of can-typed circulating pump with the new housing, and the performance of circulating pump of boiler system were tested. As a result ot the test, the new housing with internal three way valve has been excellent in respect of pump performance, weight and manufacturing cost. So It is expected to have an effect of import substitution.

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Development of the Low Cost Impedance Spectroscopy System for Modeling the Electrochemical Power Sources (전기화학적 전력 기기의 모델링을 위한 저가의 임피던스 분광 시스템의 개발)

  • Lee, Ju-Hyung;Choi, Woo-Jin
    • The Transactions of the Korean Institute of Power Electronics
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    • v.13 no.1
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    • pp.46-54
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    • 2008
  • In this paper, a low-cost impedance spectroscopy system(LCISS) suitable for modeling the electrochemical power sources such as fuel cells, batteries and supercapacitors is designed and implemented. Since the developed LCISS is composed of simple sensor circuits, commercial data acquisition board and LabVIEW software, a graphic language with powerful HMI(Human-Machine Interface), it is expected ta be widely used in substitution of the expensive EIS instruments. In the proposed system, the digital lock-in amplifier is adopted to achieve the accurate measurements even in the presence of the high level of noises. The developed hardware and software is applied to measure the impedance spectrum of the Ballard Nexa 1.2kW proton exchange membrane fuel cell stack and an equivalent impedance model is proposed based on the measurement results. The validity of the proposed equivalent circuit and the developed system is proven by the measurement of the ac power losses of the PEM fuel celt stack by the ripple current.

Broken rice in a fermented total mixed ration improves carcass and marbling quality in fattened beef cattle

  • Kotupan, Salisa;Sommart, Kritapon
    • Animal Bioscience
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    • v.34 no.8
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    • pp.1331-1341
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    • 2021
  • Objective: This study aimed to determine the effects of replacing cassava chips with broken rice in a fermented total mixed ration diet on silage quality, feed intake, ruminal fermentation, growth performance, and carcass characteristics in the final phase of fattening beef cattle. Methods: Eighteen Charolais-Thai native crossbred steers (average initial body weight: 609.4±46 kg; average age 31.6 mo) were subjected to three ad libitum dietary regimes and were maintained in individual pens for 90 d before slaughter. The experimental design was a randomized complete block design by initial age and body weight with six replicates. The dietary regimens used different proportions of broken rice (0%, 16%, and 32% [w/w] of dry matter [DM]) instead of cassava chips in a fermented total mixed ration. All dietary treatments were evaluated for in vitro gas production and tested in in vivo feeding trials. Results: The in vitro experiments indicated that organic matter from broken rice was significantly more digestible than that from a cassava-based diet (p<0.05). Silage quality, nutrient intake, ruminal fermentation characteristics, carcass fat thickness, and marbling score substantially differed among treatments. The ruminal total volatile fatty acids, propionate concentration, dietary protein intake, and digestibility increased linearly (p<0.05) with broken rice, whereas acetate concentration and the acetate:propionate ratio decreased linearly (p<0.05) with broken rice (added up to 32 g/kg DM). Broken rice did not influence plasma metabolite levels or growth performance (p>0.05). However, the marbling score increased, and the carcass characteristics improved with broken rice. Conclusion: Substitution of cassava chips with broken rice in beef cattle diets may improve fattened beef carcass quality because broken rice increases rumen fermentation, fatty acid biosynthesis, and metabolic energy supply.

Biotransformation of withanolides by cell suspension cultures of Withania somnifera (Dunal)

  • Sabir, Farzana;Sangwan, Rajender S.;Singh, Jyoti;Misra, Laxmi N.;Pathak, Neelam;Sangwan, Neelam S.
    • Plant Biotechnology Reports
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    • v.5 no.2
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    • pp.127-134
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    • 2011
  • The biotransformation potential of cell suspension cultures generated from Withania somnifera leaf was investigated, using withanolides, i.e. withanolide A, withaferin A, and withanone as precursor substrates. Interestingly, the cell suspension cultures showed inter-conversion of withanolides, as well converted to some unknown compounds, released to the culture media. The bio-catalyzed withanolide was detected and quantified by TLC and HPLC, respectively. There is noticeable conversion of withanolide A to withanone, and vice versa though at a lower level. The type of reaction of this biotransformation appears to be substitution of 20-OH group to 17-OH in withanolide A. In this paper, we present for the first time the possibility of biotransformation by inter-conversion of withanolides of pharmacological importance through cell suspension culture of W. somnifera. The possible role of putative cytochrome $P_{450}$ hydroxylases is implicated in the conversion.

Analysis of polymorphic regions of Plasmodium vivax Duffy binding protein of Korean isolates

  • Kho, Weon-Gyu;Chung, Joon-Yong;Sim, Eun-Jeong;Kim, Dong-Wook;Chung, Woo-Chul
    • Parasites, Hosts and Diseases
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    • v.39 no.2
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    • pp.143-150
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    • 2001
  • The present study was designed to investigate polymorphism in Duffy binding protein (DBP) gene of Plasmodium vivax isolates of Korea. Thirty samples were obtained from P. vivax patients in Yonchon-gun, Kyonggi-do in 1998. The PCR products of the samples were subjected to sequencing and hybridization analyses of the regions II and IV of P. vivax DBP gene. Two genotypes, SK-1 and SK-2, were identified on the basis of amino acid substitution and deletion. The genotype of 10 isolates was SK-1 and that of 20 isolates was SK-2. Most of the predicted amino acids in the region ll of DBP gene were conserved between the Korean isolates and Belem strain except for 4-5 amino acid substitutions. In the region W of DBP, a 6-bp insert that was shown in the Sal-1 allele type was found in SK-1, and a 27-bp insert that was shown in the Papua New Guinea allele type was found in SK-2. In conclusion, the present findings suggest that two genotypes of P. vivax coexist in the endemic area of Korea.

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50% Flashover Voltage and V-t Characteristics for $SF_6-N_2$ Mixtures under Positive & Negative Ligtning Impulse Voltage ($SF_6-N_2$ 혼합가스의 정.부극성 뇌충격전압에 대한 50% FOV와 V-t 특성)

  • Song, W.P.;Kim, J.D.;Koh, H.S.;Kim, D.O.;Kwon, Y.H.;Cho, K.B.;Lee, C.H.
    • Proceedings of the KIEE Conference
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    • 1991.07a
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    • pp.300-303
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    • 1991
  • Sulphur-hexafluoride has extensively been used as an insulating gas of electric power equipment in substation for its high dielectric strength, non-toixity and non-flammable properties. But it is very expensive and is liquidizable under the condition of low temperature and high pressure. And it is very sensible to the locally high electric field strength. To resolve these problems, we studied 50% FOV and V-t characteristics of $SF_6-N_2$ mixtures under positive and negative lightning impulses using a 25mm rod-plane gap. 50% FOV of $SF_6-N_2$ 50% mixture was about 85% of pure $SF_6$ and it was known that $SF_6-N_2$ mixtures can be used as an economic substitution insulating gas for $SF_6$.

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Analysis of Fuel Options in TRIGA Reactor

  • Lee, Un-Chul;Lee, Chang-Kun;Lee, Ji-Bok;Kim, Jin-Soo;Lee, Sang-Kun;Jun, Byung-Jin;Chung, Bub-Dong
    • Nuclear Engineering and Technology
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    • v.11 no.1
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    • pp.29-45
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    • 1979
  • In this paper. nuclear characteristics of TRIGA Mark-III has been analyzed in detail for six different fuel options. Presently, 70 w/o enriched FLIP fuels are adopted for TRIGA core to improve fuel lifetime. However, such highly enriched fuels are not easily obtained due to nonproliferation treaty. This research examines the possible substitution for FLIP fuels with high density fuels without reducing the nuclear performance. This work will provide long-time plan for TRIGA operation.

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A Study on the Character of Concrete compressive strength according to Bottom-Ash and Internal gap for Crack aspect predictions (Bottom-Ash를 활용한 콘크리트 압축강도와 내부 공극 특성 분석 및 균열양상 예측)

  • Jung, Woo-Young;Sim, Young-Hwan;Lee, Sang-Moon;Choi, Hyun-Kyu
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.11a
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    • pp.713-716
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    • 2008
  • In about the concrete application which recycles Ash the research came to be advanced as research in compliance with researchers relation actively in about cement substitutional concrete mixing ratio and burglar quality of existing. The research which it sees as fundamental research the research which it follows in cement substitutional concrete mixing ratio of existing and it researched different Bottom-Ash recycling qualities in about cup aggregate partial substitution Bottom-Ash application.

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