• 제목/요약/키워드: Volume quantification

검색결과 137건 처리시간 0.03초

Validation of Reduced-volume Reaction in the PowerQuant® System for human DNA Quantification

  • Kim, Hyojeong;Cho, Yoonjung;Kim, Jeongyong;Lee, Ja Hyun;Kim, Hyo Sook;Kim, Eungsoo
    • 대한의생명과학회지
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    • 제26권4호
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    • pp.275-287
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    • 2020
  • Since its introduction in the forensic field, quantitative PCR (qPCR) has played an essential role in DNA analysis. Quality of DNA should be evaluated before short tandem repeat (STR) profiling to obtain reliable results and reduce unnecessary costs. To this end, various human DNA quantification kits have been developed. Among these kits, the PowerQunat® System was designed not only to determine the total amount of human DNA and human male DNA from a forensic evidence item, but also to offer data about degradation of DNA samples. However, a crucial limitation of the PowerQunat® System is its high cost. Therefore, to minimize the cost of DNA quantification, we evaluated kit performance using a reduced volume of reagents (1/2-volume) using DNA samples of varying types and concentrations. Our results demonstrated that the low-volume method has almost comparable performance to the manufacturer's method for human DNA quantification, human male DNA quantification, and DNA degradation index. Furthermore, using a reduced volume of regents, it is possible to run 2 times more reactions per kit. We expect the proposed low-volume method to cut costs in half for laboratories dealing with large numbers of DNA samples.

An Efficient Method for Aneurysm Volume Quantification Applicable in Any Shape and Modalities

  • Chung, Jaewoo;Ko, Jung Ho
    • Journal of Korean Neurosurgical Society
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    • 제64권4호
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    • pp.514-523
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    • 2021
  • Objective : Aneurysm volume quantification (AVQ) using the equation of ellipsoid volume is widely used although it is inaccurate. Furthermore, AVQ with 3-dimensional (3D) rendered data has limitations in general use. A novel universal method for AVQ is introduced for any diagnostic modality and application to any shape of aneurysms. Methods : Relevant AVQ studies published from January 1997 to June 2019 were identified to determine common methods of AVQ. The basic idea is to eliminate the normal artery volume from 3D model with the aneurysm. After Digital Imaging and Communications in Medicine (DICOM) data is converted and exported to stereolithography (STL) file format, the 3D STL model is modified to remove the aneurysm and the volume difference between the 3D model with/without the aneurysm is defined as the aneurysm volume. Fifty randomly selected aneurysms from DICOM database were used to validate the different AVQ methods. Results : We reviewed and categorized AVQ methods in 121 studies. Approximately 60% used the ellipsoid method, while 24% used the 3D model. For 50 randomly selected aneurysms, volumes were measured using 3D Slicer, RadiAnt, and ellipsoid method. Using 3D Slicer as the reference, the ratios of mean difference to mean volume obtained by RadiAnt and ellipsoid method were -1.21±7.46% and 4.04±30.54%, respectively. The deviations between RadiAnt and 3D Slicer were small despite of aneurysm shapes, but those of ellipsoid method and 3D Slicer were large. Conclusion : In spite of inaccuracy, ellipsoid method is still mostly used. We propose a novel universal method for AVQ that is valid, low cost, and easy to use.

LII/LIS 기법을 이용한 층류확산화염 매연입자의 정량화 (Quantitative Measurements of Soot Particles in a Laminar Diffusion Flame Using a LII/LIS Technique)

  • 정재우;이원남;한용택;김병수;이춘범;김덕진;이기형
    • 한국연소학회지
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    • 제8권4호
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    • pp.1-8
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    • 2003
  • In this study, the quantification of soot particles in laminar diffusion flame with LII/LIS methods was performed. In these quantification, soot diameter, number density and volume fraction are included. For the quatification of soot particles, calibration tests are needed and the development of algorithm has to be performed. So, in this study, extinction and scattering test at co-flow burner were performed to acquire calibration data. And algorithm for LII/LIS simultaneous measurement for the quantification of soot were developed. The algorithm, which was the quantification of simultaneous photographing using one ICCD camera, to measure LII/LIS signal simultaneously, the best fitted light intensity and acquisition time was needed.

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신경회로망을 이용한 수량화 문제의 최적화 응용기법 연구 (A Study on Optimization Approach for the Quantification Analysis Problem Using Neural Networks)

  • 이동명
    • 한국정보통신학회논문지
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    • 제10권1호
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    • pp.206-211
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    • 2006
  • 수량화 문제는 n개의 성질을 갖는 m개의 개체들을 각 개체들의 유사도(similarity)를 가장 잘 반영하도록 p차원의 공간 상에 대응시키는 문제이다. 본 논문에서는 물리학에서의 열평형 상태(thermal equilibrium state)에서 분자시스템의 해석적 근사 움직임에 대한 이론인 평균장 이론(mean field theory)에 의한 분자의 평균 변화량 계산과 어닐링(annealing) 방법에 의한 평균장 신경회로망(mean field neural network)을 수량화 문제(quantification analysis problem)의 해결에 적용하였다. 그 결과, 제안한 최적화 응용기법 이 기존의 고유치 분석방법(eigen value analysis)에 비해 비용측면에서 좀 더 최적에 가까운 해답을 찾아낼 수 있음을 확인하였다.

공기 기인 소음 분석과 음향 인텐시티법을 이용한 타이어에 의한 실내 소음 예측 (Prediction of Interior Noise Caused by Tire Based on Sound Intensity and Acoustic Source Quantification)

  • 신광수;이상권;황성욱
    • 한국소음진동공학회논문집
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    • 제23권4호
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    • pp.315-323
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    • 2013
  • Tire noise is divided into a road noise(structure-borne noise) and a pattern noise(air-borne noise). Whilst the road noise is caused by the structural vibration of the components on the transfer path from tire to car body, the pattern noise is generated by the air-pumping between tire and road. In this paper, a practical method to estimate the pattern noise inside a passenger car is proposed. The method is developed based on the sound intensity and airborne source quantification. Sound intensity is used for identifying the noise sources of tire. Airborne source quantification is used for estimating the sound pressure level generated by each noise source of a tire. In order to apply the airborne source quantification to the estimation of the sound pressure, the volume velocity of each source should be obtained. It is obtained by using metrics inverse method. The proposed method is successfully applied to the evaluation of the interior noises generated by four types of tires with different pattern each other.

A new merging-zone flow injection system for the quantification of ferrous and ferric ions in aqueous solution and sludge of wastewater

  • Farhood, Ahmed Saleh;Taha, Dakhil Nassir
    • 분석과학
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    • 제35권5호
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    • pp.218-227
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    • 2022
  • A simple and fast throughput flow injection (FI) system with a merging-zone technique was designed to determine ferrous and ferric in an aqueous solution. The method is based on the direct reaction of ferrous with a Bathophenanthroline reagent (Bphen) in acidic media. The forming red complex absorbs light at 533 nm. All conditions of the flow injection system were investigated. The analytical curve of ferrous was linear in the range of 0.07 to 4 mg/L with an r2 value of 0.9968. The detection and quantification limits were 0.02 and 0.04 mg/L, respectively. The molar absorptivity and Sandell's sensitivity were 4.0577 × 106 L/mol cm and 25 × 10-5 ㎍/cm2, respectively. The homemade valve was low-cost with high repeatability (n = 7) at an RSD of 1.26 % and zero dead volume. The values of the dispersion coefficient were 2.318, 2.022, and 1.636 for the concentrations of 0.2, 1, and 3 mg/L, respectively. The analysis throughput of the designed flow injection unit was 57 sample per hour.

Multiscale simulations for estimating mechanical properties of ion irradiated 308 based on microstructural features

  • Dong-Hyeon Kwak ;Jae Min Sim;Yoon-Suk Chang ;Byeong Seo Kong ;Changheui Jang
    • Nuclear Engineering and Technology
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    • 제55권8호
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    • pp.2823-2834
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    • 2023
  • Austenitic stainless steel welds (ASSWs) of nuclear components undergo aging-related degradations caused by high temperature and neutron radiation. Since irradiation leads to the change of material characteristics, relevant quantification is important for long-term operation, but limitations exist. Although ion irradiation is utilized to emulate neutron irradiation, its penetration depth is too shallow to measure bulk properties. In this study, a systematic approach was suggested to estimate mechanical properties of ion irradiated 308 ASSW. First of all, weld specimens were irradiated by 2 MeV proton to 1 and 10 dpa. Microstructure evolutions due to irradiation in δ-ferrite and austenite phases were characterized and micropillar compression tests were performed. In succession, dislocation density based stress-strain (S-S) relationships and quantification models of irradiation defects were adopted to define phases in finite element analyses. Resultant microscopic S-S curves were compared to verify material parameters. Finally, macroscopic behaviors were calculated by multiscale simulations using real microstructure based representative volume element (RVE). Validity of the approach was verified for the unirradiated specimens such that the estimated S-S curves and 0.2% offset yield strengths (YSs) which was 363.14 MPa were in 10% agreement with test. For irradiated specimens, the estimated YS were 917.41 MPa in 9% agreement.

비글견에서 실험적으로 유발한 흉수의 정량 평가: 방사선, 컴퓨터단층촬영 및 초음파 검사 비교 (Quantification of Experimentally Induced-Pleural Effusion in Beagle Dogs: Radiography versus CT and Ultrasonography)

  • 이기자;오이세;정성목;이희천;박성준;이영원;최호정
    • 한국임상수의학회지
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    • 제25권2호
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    • pp.96-101
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    • 2008
  • 흉수량은 흉수천자의 실시 여부 및 진단과 치료에 이용되는 방법의 결정에 영향을 미치므로 흉수량 측정은 중요하다. 본 실험의 목적은 흉수를 정량화하기 위해 흉부 방사선 및 초음파와 컴퓨터단층촬영을 시행하여 그 결과를 비교하고 정확도를 측정하는데 있다. 임상적으로 건강한 6 마리의 비글견을 이용하여 생리식염수를 최종 용량이 60 ml/kg 될 때까지 체중당 10ml씩 흉강에 주입하면서 흉부 방사선 검사, 초음파 검사, CT 검사를 차례로 실시하였다. 흉부 방사선 사진에서 흉수의 유무 평가 및 흉수 정량화를 실시하였으며 초음파 영상에서 흉수량은 폐의 표면과 흉강벽 사이의 최대 수직 거리를 측정하여 평가하였다. CT 영상에서 흉수량은 soft tissue window에서 평가하였으며 최대 흉수량을 나타내는 영상에서 최소 수직거리를 측정하였다. 흉수량과 흉부 방사선 사진 및 초음파 영상에서의 측정치와 통계학적으로 유의적인 결과를 얻지 못했다. 그러나 CT 검사에서의 측정값과 흉수량과의 유의적인 관계를 나타내었다. 특히 흉수의 정량화에서 좌우측 측정치의 평균값이 좌우측 각각의 값보다 더 높은 정확도를 나타내었다. 흉수를 확인하기 위해서는 흉부 방사선 검사 초음파 검사 및 CT 검사가 모두 가능하나 흉수를 정량화하기 위해서는 흉부 방사선 및 초음파 검사보다 CT 검사가 유용함을 확인하였다.

Quantification of Volumetric In-Cylinder Flow of SI Engine Using 3-D Laser Doppler Velocimetry ( II )

  • Yoo, Seoung-Chool
    • 한국유체기계학회 논문집
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    • 제10권4호
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    • pp.47-54
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    • 2007
  • Simultaneous 3-D LDV measurements of the in-cylinder flows of three different engine setups were summarized for the quantification of the flow characteristics in each vertical or horizontal plane, and in entire cylinder volume. The ensemble averaged-velocity, tumble and swirl motions, and turbulent kinetic energy during the intake and compression strokes were examined from the measured velocity data (approximately 2,000 points for each engine setup). The better spatial resolution of the 3-D LDV allows measurements of the instantaneous flow structures, yielding more valuable information about the smaller flow structures and the cycle-to-cycle variation of these flow patterns. Tumble and swirl ratios, and turbulent kinetic energy were quantified as planar and volumetric quantities. The measurements and calculation results were animated for the visualization of the flow, and hence ease to analysis.