• Applied Microscopy
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• 제19권2호
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• pp.85-98
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• 1989

The ventricular myocardia of 14, 16, 18 and 20-day-old rat fetuses and newborns have been studies by light and electron microscopic morphometrics. The volume density of the myocyte and interstitial compartments as well as volume, surface and numerical density of nuclei were estimated by light microscopic morphometrics. Whereas, the volume density of myofibrils and glycogen granules as well as the volume, surface and numerical density of mitochondria were assessed by electron microscopic morphometrics. The volume density of myocyte compartment of the ventricular myocardia in developing fetuses decreased, but increased in newborn rats. On the other hand, the volume density of the interstitial compartment increased in growing fetuses and decreased in newborns. In all groups the volume, surface and numerical density of nuclei decreased gradually with elongation of myocytes. Conversely, the volume, surface and numerical density of mitochondria and volume density of myofibrils and glycogen granules in ventricular myocytes incresed. The increase in numerical density of mitochondria probably reflects an increase in metabolic activity. Sarcomere length also increased during development.

• Applied Microscopy
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• 제22권1호
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• pp.42-54
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• 1992

To understand the structural changes of the myocardial myocytes and endothelial cells in ischemic and reperfused heart, and to elucidate their roles in those conditions, the authors observed cat and rat myocardium ultrastructurally and evaluated them with morphometric techniques. In cat, mild ischemia and moderate degree reperfusion injury was induced by ligation of the anterior interventricular branch of left coronary artery and reperfusion. In rat, severe ischemia and irreversible reperfusion iniury was made using in vitro Langendorff techniques. In normal cat myocytes, the volume densities of cytoplasm, myofibrils, mitochondria, sarcoplasmic reticulum and T tubules were $0.11{\pm}0.013,\;0.51{\pm}0.096,\;0.25{\pm}0.082,\;0.09{\pm}0.008,\;0.02{\pm}0.010$ (Mean${\pm}$S.D.) respectively, and the myofibril/mitochondria ratio was $2.33{\pm}1.379$. The numerical density and average volume of mitochondria were $0.76{\pm}0.210/{\mu}m^3$ and $0.33{\pm}0.057{\mu}m^3$ respectively. In normal cat endothelial cells, the volume densities of cytoplasm, cytoplasmic vesicles, tubular systems (including endoplasmic reticulum and Golgi apparatus) and mitochondria were $0.43{\pm}0.023,\;0.28{\pm}0.007,\;0.22{\pm}0.021,\;0.03{\pm}0.014$ respectively. The mean thickness of endothelial cells was $230{\pm}45.2{\mu}m$. The numerical density and average volume of cytoplasmic vesicles were $508{\pm}55.0/{\mu}m^3,\;578{\pm}104.8nm^3$ respectively. In cat myocytes which received mild ischemic injury, the volume densities of organelles were not changed significantly in ischemic and reperfusion states. In reperfusion group myocytes, the numerical density of mitochondria was decreased significantly and the average volume was increased significantly. In endothelial cells, the volume density of tubular system in ischemic group and the average volume of cytoplasmic vesicles in reperfusion group were increased significantly. In rat myocytes which received severe ischemic injury, the volume density and average volume of mitochondria were increased significantly, and the volume density of sarcoplasmic reticulum and numerical density of mitochondria were decreased significantly in both ischemic and reperfusion groups. In ischemic and reperfused endothelial cells, the volume density and numerical density of cytoplasmic vesicles, the volume density of cytoplasm were decreased significantly. The volume densities of tubular system were increased significantly in both ischemic and reperfused groups. The volume density of mitochondria in ischemic group and the average volume of cytoplasmic vesicles in reperfusion group showed significant increase. The authors, based on the above observations, conclude that the mitochondria of myocytes and the cytoplasmic vesicles of endothelia are the first group of targets in ischemic and reperfusion injury and in this respect, the degree of ischemic insult is not significant. The role of myocyte mitochondria in reperfusion injury may be insignificant, but endothelial cells may contribute actively to reperfusion injury.

• 한국임상수의학회지
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• 제28권5호
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• pp.510-515
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• 2011

개에서 컴퓨터단층촬영술을 이용하여, 촬영 자세에 따른 정상 폐의 용적과 밀도를 정량적으로 평가하였다. 각각 6 마리의 페키니즈견과 말티즈견에서 컴퓨터단층촬영을 실시하면서 자세를 앙와위, 좌측과 우측 횡와위, 그리고 흉와위로 변경하였다. 컴퓨터단층촬영을 실시하는 동안 일정한 정도의 양압환기를 실시하였다. 폐용적과 밀도는 CT 영상의 삼차원 재구성 방식을 통해 측정하였고, 전체 폐용적 및 밀도, 좌측과 우측 폐의 용적과 밀도를 각각 측정하였다. 삼차원 재구성 결과 폐의 모양은 두 품종견에서 매우 다르게 나타났다. 폐용적과 밀도는 촬영 자세를 변경하는 동안 두 품종견에서 모두 유의적인 변화를 보이지 않았다. 오른쪽 폐용적이 왼쪽 폐에 비해 유의적으로 약 $66.91{\pm}25.1$ ml 만큼 컸다. 페키니즈 견과 말티즈 견의 체중 차이가 유의적이었으며, 체중과 흉와위로 촬영한 폐용적 간에 선형관계가 관찰되었다. 좌우측 횡와위 촬영에서 중력의 영향을 받는 쪽의 폐는 반대쪽 폐에 비해 밀도는 높고 용적은 감소되었다. 컴퓨터단층촬영으로 측정한 전체 폐용적은 폐밀도와 관련이 있었으며, 폐밀도가 정상 폐용적을 유추하는데 유용하게 사용될 것으로 생각된다.

• 한국가금학회지
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• 제27권1호
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• pp.63-71
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• 2000

The testis is an extremely heterogeneous organ, containing numerous compartments types. Morphometric studies were performed of 3 avian species (pigeon, pheasant and chicken) to determine volume density absolute volume, numerical density, total number of serminiferous tubule components, and sperm production, especially those related to the Sertoli cell, and to make comparisons among the species. Volume density of seminiferous tubule components per testis was determined by point counting method. Testis volume and sperm production were measured by routine techniques. Numerical density (the number of cells per unit volume of testis) of seminiferous tubule components per testis was determined by morphometry (Floderus method). The volume density of seminiferous tubules per testis was 91.58, 92.18 and 94.21% in pigeon, pheasant, and chicken, respectively. The volume density of spermatogonium, spermatocyte, spermatid, spermatozoon, and Sertoli cell did not produce significant changes in the three species. The absolute volume of spermatogonium, spermatocyte, spermatid, and Sertoli cell showed significant changes in the three species (p<0.05). The average volume of Sertoli cell ranged from 758.34(pheasant) to 1,212.9 ㎛$^3$(chicken) and was not significantoy different in the three species(p>0.05). The number of Sertoli cells per testis showed significant differences in the three species : 34.52 $\times$10(sup)6, 186.82$\times$10(sup)6, 810.62$\times$10(sup)6 in pigeon, pheasant, and chicken, respectively(p<0.05). The sperm production was significantly different in the three species : 3,018$\times$10(sup)6, 993.9$\times$10(sup)6, and 8.9$\times$10(sup)6 in chicken, pheasant, and pigeon, respectively(p<0.05). These results suggest that number of Sertoli cells may be more important than Sertoli cell size in explaining the difference in sperm production among the three species.

• 한국물환경학회지
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• 제22권2호
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• pp.264-270
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• 2006

In this research, volume reduction of activated sludge using electrolysis was studied to find an optimum condition using lab scale experiments. Wasted sludge was treated by electrolysis with controlling current density, chloride concentration, electrode distance, and reaction time. Volume of return sludge was reduced by 9.79% in average while maximum was 16.7%. Sludge volume reduction efficiency was affected by current density and reaction time. It was reversely proportional to the electrode distance. Especially current density was effective on the system performance significantly. Electric conductivity, salinity and COD were increased by electrolysis implying sludge disintegrated and converted to COD in part. An empirical equation for total solid removal efficiency by electrolysis was proposed by multiple linear regression analysis as: $TS_{rem}$(%) = 5.534 ${\times}$ current density (A/l) + 0.178 ${\times}$ reaction time (m) + 2.758.

• 한국토양비료학회지
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• 제48권5호
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• pp.505-508
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• 2015

Soil bulk density is a key parameter for soil physical property. Much root placed in rhizosphere soil lump, especially in grassland and orchard, makes it difficult to measure soil bulk density. This experiment was carried out to countermeasure the above drawbacks. Volume check apparatus using water-filling method was made of acryl for higher accuracy in bulk density measurement. 10 types of land cover, including bare, tall fescue, rye, and soybean, were used for determining the relationships between root and bulk density. In this study, higher root volume resulted in higher differences in bulk density between in-situ core soil and root-ridded core soil, which indicated the volume check apparatus through water-filling could be useful for increasing the accuracy of bulk density of soils with much root.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2010년도 춘계 학술발표회
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• pp.717-721
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• 2010

Soil volume conversion factors are used for estimation of an excavated the soil volume which will be removed or added in levelling the ground surface of a construction site. An accurate evaluation method will help us reduce a construction cost and time consuming. In this study, we performed the laboratory tests, including grain size measurement, water content, specific gravity, porosity, density and XRD tests, to suggest reliable soil volume conversion factors and weathering indices in field using nondestructive methods. The weathering index and soil volume conversion factor L are obtained for different types of soils. At results, the CIW index is the best method measuring the weathering index and the factor L is relative to natural porosity, void ratio, density and dry density.

• 한국지반신소재학회논문집
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• 제16권1호
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• pp.9-17
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• 2017

본 연구에서는 암성토의 토공량 설계에 대한 대책방안으로 현장밀도시험을 수행하였으며, 현장밀도시험결과 암성토부의 발파암 평균 체적환산계수는 1.001로서 풍화암의 체적환산계수 1.1보다 작은 값이 나타났다. 이와 같이 암성토의 경우 여러 가지 현상에 의해 토공량의 증감 원인을 제공하고 있으나, 이에 대한 조사 및 시험방법 등 구체적인 기술 등이 부족한 실정이므로 본 연구결과에서와 같이 체적환산계수를 검토하기 위한 조사방법으로 현장밀도시험방법이 매우 유용한 방법임을 확인하였다.

• 대한수학회보
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• 제60권6호
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• pp.1539-1553
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• 2023

We show the asymptotics of the volume density function in the class of central harmonic manifolds can be specified arbitrarily and do not determine the geometry.

• Journal of Advanced Marine Engineering and Technology
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• 제33권3호
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• pp.378-386
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• 2009

The effect of fuel mixing on soot structure with methane, ethane, and propane to ethylene-base counterflow diffusion flames has been investigated by measuring the volume fraction, number density, and particle size of soot by adopting the light extinction/scattering techniques. The experimental result showed that the mixing of ethane and propane in ethylene diffusion flame increased soot volume fraction while the mixing of methane decreased. As compare to the ethylene-base flame, the diameters of soot particles for mixture flames are slightly smaller. While the soot number densities for the mixture flames are much higher. Thus, the increase in the soot volume fraction can be attributed to the appreciably increased soot number density by the fuel mixing.