• Title/Summary/Keyword: Volume density

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Morphometric Study of Heart Development in Rat Fetus (Rat 태생기의 심장성장에 따른 형태측량적 연구)

  • Park, Won-Hark;Lee, Yong-Deok;Chung, Hyeung-Jae;Choi, Jeung-Mog
    • Applied Microscopy
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    • v.19 no.2
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    • pp.85-98
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    • 1989
  • The ventricular myocardia of 14, 16, 18 and 20-day-old rat fetuses and newborns have been studies by light and electron microscopic morphometrics. The volume density of the myocyte and interstitial compartments as well as volume, surface and numerical density of nuclei were estimated by light microscopic morphometrics. Whereas, the volume density of myofibrils and glycogen granules as well as the volume, surface and numerical density of mitochondria were assessed by electron microscopic morphometrics. The volume density of myocyte compartment of the ventricular myocardia in developing fetuses decreased, but increased in newborn rats. On the other hand, the volume density of the interstitial compartment increased in growing fetuses and decreased in newborns. In all groups the volume, surface and numerical density of nuclei decreased gradually with elongation of myocytes. Conversely, the volume, surface and numerical density of mitochondria and volume density of myofibrils and glycogen granules in ventricular myocytes incresed. The increase in numerical density of mitochondria probably reflects an increase in metabolic activity. Sarcomere length also increased during development.

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A Quantitative Ultrastructural Study on the Effects of Ischemia and Reperfusion on the Rat and Cat Hearts (허혈 및 재관류가 흰쥐 및 고양이 심장에 미치는 영향에 관한 형태계측학적 연구)

  • Park, Young-Sik;Uhm, Chang-Sub;Suh, Young-Suk
    • Applied Microscopy
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    • v.22 no.1
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    • pp.42-54
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    • 1992
  • To understand the structural changes of the myocardial myocytes and endothelial cells in ischemic and reperfused heart, and to elucidate their roles in those conditions, the authors observed cat and rat myocardium ultrastructurally and evaluated them with morphometric techniques. In cat, mild ischemia and moderate degree reperfusion injury was induced by ligation of the anterior interventricular branch of left coronary artery and reperfusion. In rat, severe ischemia and irreversible reperfusion iniury was made using in vitro Langendorff techniques. In normal cat myocytes, the volume densities of cytoplasm, myofibrils, mitochondria, sarcoplasmic reticulum and T tubules were $0.11{\pm}0.013,\;0.51{\pm}0.096,\;0.25{\pm}0.082,\;0.09{\pm}0.008,\;0.02{\pm}0.010$ (Mean${\pm}$S.D.) respectively, and the myofibril/mitochondria ratio was $2.33{\pm}1.379$. The numerical density and average volume of mitochondria were $0.76{\pm}0.210/{\mu}m^3$ and $0.33{\pm}0.057{\mu}m^3$ respectively. In normal cat endothelial cells, the volume densities of cytoplasm, cytoplasmic vesicles, tubular systems (including endoplasmic reticulum and Golgi apparatus) and mitochondria were $0.43{\pm}0.023,\;0.28{\pm}0.007,\;0.22{\pm}0.021,\;0.03{\pm}0.014$ respectively. The mean thickness of endothelial cells was $230{\pm}45.2{\mu}m$. The numerical density and average volume of cytoplasmic vesicles were $508{\pm}55.0/{\mu}m^3,\;578{\pm}104.8nm^3$ respectively. In cat myocytes which received mild ischemic injury, the volume densities of organelles were not changed significantly in ischemic and reperfusion states. In reperfusion group myocytes, the numerical density of mitochondria was decreased significantly and the average volume was increased significantly. In endothelial cells, the volume density of tubular system in ischemic group and the average volume of cytoplasmic vesicles in reperfusion group were increased significantly. In rat myocytes which received severe ischemic injury, the volume density and average volume of mitochondria were increased significantly, and the volume density of sarcoplasmic reticulum and numerical density of mitochondria were decreased significantly in both ischemic and reperfusion groups. In ischemic and reperfused endothelial cells, the volume density and numerical density of cytoplasmic vesicles, the volume density of cytoplasm were decreased significantly. The volume densities of tubular system were increased significantly in both ischemic and reperfused groups. The volume density of mitochondria in ischemic group and the average volume of cytoplasmic vesicles in reperfusion group showed significant increase. The authors, based on the above observations, conclude that the mitochondria of myocytes and the cytoplasmic vesicles of endothelia are the first group of targets in ischemic and reperfusion injury and in this respect, the degree of ischemic insult is not significant. The role of myocyte mitochondria in reperfusion injury may be insignificant, but endothelial cells may contribute actively to reperfusion injury.

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Evaluation of Total Lung Volume and Density using Multi-Detector Computed Tomography in Normal Dogs (정상견에서 다중채널 컴퓨터단층촬영술을 통한 폐용적과 밀도의 평가)

  • Choi, Ho-Jung;Lee, Ki-Ja;Choi, Soo-Young;Lee, Jung-Woo;Han, Woo-Sok;Lee, In;Kwon, Young-Hang;Lee, Young-Won
    • Journal of Veterinary Clinics
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    • v.28 no.5
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    • pp.510-515
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    • 2011
  • This study was performed to quantitatively assess the normal lung volume and density according to the position by multi-detector computed tomography (MDCT) in dogs. Helical CT of the thorax was performed on 4 different positions with dorsal, left lateral, right lateral and ventral recumbency in 6 Pekingese and 6 Maltese dogs. During CT scanning, dogs were kept hyperventilated. Through the 3-dimensional reconstruction of CT images, the lung parameters were measured as the volume and density of the left, right including accessory lobe, and total lung. 3D images represented the different lung shape between Pekingese and Maltese dogs. Their difference of total lung volume and total lung density was not significant on the each position in both breeds. Right lung volume was significantly higher than left. The difference of left and right volume was $66.91{\pm}25.1$ ml. Linear relationship was shown between body weight and lung volume of ventral recumbency position. The dependent lung had higher density and lower volume than nondependent lung in both breed dogs. The volume of nondependent lung was not changed compared with the volume on ventral or dorsal recumbency. The total lung volume measured with MDCT is correlated with the lung density, and the lung density is useful to predict the normal total lung volume.

Morphometric Study of Seminiferous Tubules in Pigeon, Pheasant, and Chicken (비둘기, 꿩 및 닭의 곱슬정세관에 관한 형태계측학적 연구)

  • 김인식;김지현;이영훈;정옥봉;양홍현
    • Korean Journal of Poultry Science
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    • v.27 no.1
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    • pp.63-71
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    • 2000
  • The testis is an extremely heterogeneous organ, containing numerous compartments types. Morphometric studies were performed of 3 avian species (pigeon, pheasant and chicken) to determine volume density absolute volume, numerical density, total number of serminiferous tubule components, and sperm production, especially those related to the Sertoli cell, and to make comparisons among the species. Volume density of seminiferous tubule components per testis was determined by point counting method. Testis volume and sperm production were measured by routine techniques. Numerical density (the number of cells per unit volume of testis) of seminiferous tubule components per testis was determined by morphometry (Floderus method). The volume density of seminiferous tubules per testis was 91.58, 92.18 and 94.21% in pigeon, pheasant, and chicken, respectively. The volume density of spermatogonium, spermatocyte, spermatid, spermatozoon, and Sertoli cell did not produce significant changes in the three species. The absolute volume of spermatogonium, spermatocyte, spermatid, and Sertoli cell showed significant changes in the three species (p<0.05). The average volume of Sertoli cell ranged from 758.34(pheasant) to 1,212.9 ㎛$^3$(chicken) and was not significantoy different in the three species(p>0.05). The number of Sertoli cells per testis showed significant differences in the three species : 34.52 $\times$10(sup)6, 186.82$\times$10(sup)6, 810.62$\times$10(sup)6 in pigeon, pheasant, and chicken, respectively(p<0.05). The sperm production was significantly different in the three species : 3,018$\times$10(sup)6, 993.9$\times$10(sup)6, and 8.9$\times$10(sup)6 in chicken, pheasant, and pigeon, respectively(p<0.05). These results suggest that number of Sertoli cells may be more important than Sertoli cell size in explaining the difference in sperm production among the three species.

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Volume Reduction of Waste Water Sludge using Electrolysis (전기분해를 이용한 하수 슬러지 감량)

  • Lee, Byungheon;Bang, Myunghwan;Kim, Geonha
    • Journal of Korean Society on Water Environment
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    • v.22 no.2
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    • pp.264-270
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    • 2006
  • In this research, volume reduction of activated sludge using electrolysis was studied to find an optimum condition using lab scale experiments. Wasted sludge was treated by electrolysis with controlling current density, chloride concentration, electrode distance, and reaction time. Volume of return sludge was reduced by 9.79% in average while maximum was 16.7%. Sludge volume reduction efficiency was affected by current density and reaction time. It was reversely proportional to the electrode distance. Especially current density was effective on the system performance significantly. Electric conductivity, salinity and COD were increased by electrolysis implying sludge disintegrated and converted to COD in part. An empirical equation for total solid removal efficiency by electrolysis was proposed by multiple linear regression analysis as: $TS_{rem}$(%) = 5.534 ${\times}$ current density (A/l) + 0.178 ${\times}$ reaction time (m) + 2.758.

Effects of Root on Bulk Density of Soils Tested by Volume Check Apparatus through Water-filling

  • Lee, Gye-Jun;Lee, Jeong-Tae;Ryu, Jong-Soo;Oh, Dong-Shig;Kim, Jeom-Soon;Lee, Yeong-Gyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.48 no.5
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    • pp.505-508
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    • 2015
  • Soil bulk density is a key parameter for soil physical property. Much root placed in rhizosphere soil lump, especially in grassland and orchard, makes it difficult to measure soil bulk density. This experiment was carried out to countermeasure the above drawbacks. Volume check apparatus using water-filling method was made of acryl for higher accuracy in bulk density measurement. 10 types of land cover, including bare, tall fescue, rye, and soybean, were used for determining the relationships between root and bulk density. In this study, higher root volume resulted in higher differences in bulk density between in-situ core soil and root-ridded core soil, which indicated the volume check apparatus through water-filling could be useful for increasing the accuracy of bulk density of soils with much root.

Estimation of Soil Volume Conversion Factors using Nondestructive Testing Methods (비파괴시험기법을 이용한 토량환산계수 산정 방법 제시)

  • Son, Thai An;Ryu, Hee-Hwan;Cho, Gye-Chun;Hong, Eun-Soo;Jin, Gyu-Nam
    • Proceedings of the Korean Geotechical Society Conference
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    • 2010.03a
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    • pp.717-721
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    • 2010
  • Soil volume conversion factors are used for estimation of an excavated the soil volume which will be removed or added in levelling the ground surface of a construction site. An accurate evaluation method will help us reduce a construction cost and time consuming. In this study, we performed the laboratory tests, including grain size measurement, water content, specific gravity, porosity, density and XRD tests, to suggest reliable soil volume conversion factors and weathering indices in field using nondestructive methods. The weathering index and soil volume conversion factor L are obtained for different types of soils. At results, the CIW index is the best method measuring the weathering index and the factor L is relative to natural porosity, void ratio, density and dry density.

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The Estimation of Volume Conversion Factor of Rock Fill using Field Density Test (현장밀도시험을 이용한 암성토 체적환산계수 산정에 관한 연구)

  • Park, Jong-Beom;Ma, Myong-Soo
    • Journal of the Korean Geosynthetics Society
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    • v.16 no.1
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    • pp.9-17
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    • 2017
  • In this study, the field density test was conducted as a volume conversion factor for the design of the excavation soil of the blasting rock. As a result of the field density test, the average volume conversion factor of rock was 1.001, which was smaller than the volume conversion factor of weathered rock 1.1. In the case of rock filled soil, the causes of the increase and decrease of the volume of the soil are provided by various phenomena. However, the specific techniques such as investigation and test methods are insufficient. Therefore, it was confirmed that the method of field density test is very useful method.

Experimental Study on Characteristics of Synergistic Effect of Fuel Mixing on Number Density and Size of Soot in Ethylene-base Counterflow Diffusion Flames by Laser Techniques

  • Choi, Jae-Hyuk
    • Journal of Advanced Marine Engineering and Technology
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    • v.33 no.3
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    • pp.378-386
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    • 2009
  • The effect of fuel mixing on soot structure with methane, ethane, and propane to ethylene-base counterflow diffusion flames has been investigated by measuring the volume fraction, number density, and particle size of soot by adopting the light extinction/scattering techniques. The experimental result showed that the mixing of ethane and propane in ethylene diffusion flame increased soot volume fraction while the mixing of methane decreased. As compare to the ethylene-base flame, the diameters of soot particles for mixture flames are slightly smaller. While the soot number densities for the mixture flames are much higher. Thus, the increase in the soot volume fraction can be attributed to the appreciably increased soot number density by the fuel mixing.