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Effect of pH on Calcium-Activated Potassium Channels in Pulmonary Arterial Smooth Muscle Cells of the Rabbit

  • Lee, Suk-Ho;Ho, Won-Kyung;Earm, Yung-E
    • The Korean Journal of Physiology
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    • v.25 no.1
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    • pp.17-26
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    • 1991
  • Single smooth muscle cells of the rabbit pulmonary artery were isolated by treatment with collagenase and elastase. Using the patch clamp technique, potassium channel activity was recorded from the inside-out membrane patch. The channel had a sin히e channel conductance of about 360 pS in symmetrical concentration of K on both sides of the patch, 150 mM, and had a linear current-voltage relationship. During the application of 10 mM tetraethylammonium (TEA) to the intracellular membrane surface, the amplitude of single channel current was reduced and very rapid flickering appeared. The open probability $(P_0)$ of this channel was increased by increasing positivity of the potential across the patch membrane, with e-fold increase by 20 mV depolarization, and by increasing the internal $Ca^{2+}$ concentration. These findings are consistent with those of large conductance Ca-activated K channels reported in other tissues. But the shortening of the mean open time by increasing $[Ca^{2+}]_i$, was an unexpected result and one additional closed state which might be arisen from a block of the open channel by Ca binding was suggested. The $P_0-membrane$ potential relationship was modulated by internal pH. Decreasing pH reduced $P_0$. Increasing pH not only increased $P_0$ but also weakened the voltage dependency of the channel opening. The modulation of Ca-activated K channel by pH was thought to be related to the mechanism of regulation of vascular tone by the pH change.

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Recrystallized poly-Si TFTs on metal substrate (금속기판에서 재결정화된 규소 박막 트랜지스터)

  • 이준신
    • Electrical & Electronic Materials
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    • v.9 no.1
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    • pp.30-37
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    • 1996
  • Previously, crystallization of a-Si:H films on glass substrates were limited to anneal temperature below 600.deg. C, over 10 hours to avoid glass shrinkage. Our study indicates that the crystallization is strongly influenced by anneal temperature and weakly affected by anneal duration time. Because of the high temperature process and nonconducting substrate requirements for poly-Si TFTs, the employed substrates were limited to quartz, sapphire, and oxidized Si wafer. We report on poly-Si TFT's using high temperature anneal on a Si:H/Mo structures. The metal Mo substrate was stable enough to allow 1000.deg. C anneal. A novel TFT fabrication was achieved by using part of the Mo substrate as drain and source ohmic contact electrode. The as-grown a-Si:H TFT was compared to anneal treated poly-Si TFT'S. Defect induced trap states of TFT's were examined using the thermally stimulated current (TSC) method. In some case, the poly-Si grain boundaries were passivated by hydrogen. A-SI:H and poly-Si TFT characteristics were investigated using an inverted staggered type TFT. The poly -Si films were achieved by various anneal techniques; isothermal, RTA, and excimer laser anneal. The TFT on as grown a-Si:H exhibited a low field effect mobility, transconductance, and high gate threshold voltage. Some films were annealed at temperatures from 200 to >$1000^{\circ}C$ The TFT on poly-Si showed an improved $I_on$$I_off$ ratio of $10_6$, reduced gate threshold voltage, and increased field effect mobility by three orders. Inverter operation was examined to verify logic circuit application using the poly Si TFTs.

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Effects of NaOH Concentration on the Structure of PEO Films Formed on AZ31 Mg Alloy in PO43- and SiO32- Containing Aqueous Solution (인산 및 규산 이온이 포함된 수용액에서 AZ31 마그네슘 합금의 플라즈마 전해산화 피막의 구조에 미치는 수산화나트륨 농도의 영향)

  • Kwon, Duyoung;Moon, Sungmo
    • Journal of the Korean institute of surface engineering
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    • v.49 no.1
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    • pp.46-53
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    • 2016
  • The structure of plasma electrolytic oxidation (PEO) coatings was investigated as a function of NaOH concentration in 0.06 M $Na_2SiO_3$ + 0.06 M $Na_3PO_4$ solution by using SEM and epoxy replica method. The PEO film was formed on AZ31 Mg alloy by the application of anodic pulse current with 0.2 ms width and its formation behavior was studied by voltage-time curves during the formation of PEO films. It was found that the addition of NaOH into $PO_4{^{3-}}$ and $SiO_3{^{2-}}$ containing aqueous solution causes a decrease in the PEO film formation voltage, suggesting that dielectric breakdown of the PEO becomes easier with increasing $OH^-$ ion concentration in the solution. With increasing $OH^-$ ion concentration, thickness of the PEO film increased and surface roughness decreased. The size of pores formed in the PEO layer became smaller and the number of cracks in the PEO layer increased with increasing $OH^-$ ion concentration. Based on the experimental results obtained in the work, it is suggested that $OH^-$ ions in the solution can contribute not only to the dielectric breakdown but also to the formation of PEO films in the presence of $PO_4{^{3-}}$ and $SiO_3{^{2-}}$ ions in the solution.

Effect of Na3PO4 Concentration on The Formation Behavior of PEO films on AZ31 Mg Alloy (AZ31 Mg합금의 PEO피막 형성거동에 미치는 인산나트륨 농도의 영향)

  • Moon, Sungmo;Kim, Juseok
    • Journal of the Korean institute of surface engineering
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    • v.52 no.5
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    • pp.265-274
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    • 2019
  • Formation behavior of PEO (Plasma Electrolytic Oxidation) films on AZ31 Mg alloy was investigated under application of 310 Hz AC as a function of $Na_3PO_4$ concentration from 0.02 M to 0.2 M. Film formation voltage and in-situ observation of arcs generated on the specimen surface were recorded with time, and surface morphologies of the PEO films were investigated using optical microscopy, confocal scanning laser microscopy and scanning electron microscopy. PEO film formation voltage decreased linearly with increasing $Na_3PO_4$ concentration which is attributed to the increase of solution pH. PEO films were grown uniformly over the entire surface in $Na_3PO_4$ solutions between 0.05 M and 0.1 M. However, non-uniform PEO films with white spots were formed in $Na_3PO_4$ solutions containing more than 0.1 M. Thickness and roughness of PEO films on AZ31 Mg alloy increased linearly with increasing $Na_3PO_4$ concentration and their increasing rates appeared to be much higher under 1 M than above 1 M. The experimental results suggest that phosphate ions can contribute to the formation of PEO films but higher $Na_3PO_4$ concentration more than 1 M results in local damages of PEO films due to repeated generation of white arcs at the same surface site of AZ31 Mg alloy.

Selective serotonin reuptake inhibitor escitalopram inhibits 5-HT3 receptor currents in NCB-20 cells

  • Park, Yong Soo;Sung, Ki-Wug
    • The Korean Journal of Physiology and Pharmacology
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    • v.23 no.6
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    • pp.509-517
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    • 2019
  • Escitalopram is one of selective serotonin reuptake inhibitor antidepressants. As an S-enantiomer of citalopram, it shows better therapeutic outcome in depression and anxiety disorder treatment because it has higher selectivity for serotonin reuptake transporter than citalopram. The objective of this study was to determine the direct inhibitory effect of escitalopram on 5-hydroxytryptamine type 3 ($5-HT_3$) receptor currents and study its blocking mechanism to explore additional pharmacological effects of escitalopram through $5-HT_3$ receptors. Using a wholecell voltage clamp method, we recorded currents of $5-HT_3$ receptors when 5-HT was applied alone or co-applied with escitalopram in cultured NCB-20 neuroblastoma cells known to express $5-HT_3$ receptors. 5-HT induced currents were inhibited by escitalopram in a concentration-dependent manner. $EC_{50}$ of 5-HT on $5-HT_3$ receptor currents was increased by escitalopram while the maximal peak amplitude was reduced by escitalopram. The inhibitory effect of escitalopram was voltage independent. Escitalopram worked more effectively when it was co-applied with 5-HT than pre-application of escitalopram. Moreover, escitalopram showed fast association and dissociation to the open state of $5-HT_3$ receptor channel with accelerating receptor desensitization. Although escitalopram accelerated $5-HT_3$ receptor desensitization, it did not change the time course of desensitization recovery. These results suggest that escitalopram can inhibit $5-HT_3$ receptor currents in a non-competitive manner with the mechanism of open channel blocking.

Design of Simulated Photovoltaic Power Streetlight for Education using Renewable Energy Utilization and Storage Function (신재생에너지 활용 및 저장기능을 이용한 교육용 모의 태양광발전 가로등 설계)

  • Yoon, Yongho
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.21 no.2
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    • pp.137-142
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    • 2021
  • A Photovoltaic power streetlight is a system that uses solar energy to charge a secondary battery and then uses it for night lighting through a lamp, and can be configured as a standalone or grid-connected type by installing an LED streetlight at the load end. The energy generated through the solar cell module can be charged to the secondary battery through the charge/discharge control device, and then the LED street light can be turned on and off by comparing the power generation voltage and the charging voltage according to the monitoring of solar radiation, or by setting a specific time after sunset or sunrise. Based on these contents, this paper designed and manufactured a simulated solar power streetlight for education using new and renewable energy utilization and storage functions. Using these educational equipment, students can 1) understand the flow of energy change using renewable energy including sunlight as electric energy, 2) understand new and renewable energy, and cultivate basic design and manufacturing application power of related products, 3) The use of new and renewable energy through power conversion and strengthening of practical training and analysis through hardware production can be instilled.

Plant diversity of the pads of electric towers along the deltaic Mediterranean coast of Egypt

  • Kamal Shaltout;Hani Beshara;Yassin Al-Sodany;Ahmed Sharaf, El-Din;Ragab El-Fahar
    • Journal of Ecology and Environment
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    • v.47 no.3
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    • pp.63-74
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    • 2023
  • Background: Comparing with the several types of infrastructures, linear infrastructures are known to facilitate the spread of undesirable species in ecosystems. Recently, some new man-made habitats (e.g., gravel pads of the high-voltage towers, solid wastes and sewage habitats) were established along the Deltaic Mediterranean coast of Egypt as a result of the construction of the E-W coastal international highway. The current study evaluates the floristic composition associated with the pads of high-voltage towers that had been constructed for stabilizing the power line towers in the North Nile Delta. Plant cover was measured for 22 randomly stand. Results: Eighty-four species were recorded, of which 35 are perennials (41.6%), 2 biennials (2.3%) and 47 annuals (56.0%) belonging to 23 families. The largest families were Asteraceae (16 species), Poaceae (15 species), Chenopodiaceae (12 species), and Fabaceae (7 species). Ten aliens (10.7%) out of the 84 species were recorded. Therophytes have the highest percentage (58%), followed by hemicryptophytes (14%), chamaephytes (11%). Six vegetation groups were recognized in the study area after the application of two way indicator species analysis (TWINSPAN), Arthrocnemum macrostachyum, Phragmites australis, and Mesembryanthemum nodiflorum have the highest presence percentage. Both of Salsola cyclophylla and Solanum villosum were recorded for the first time in North Nile Delta. Natural habitat had the highest α-diversity, but the lowest β-diversity (4.9, 15.4), while gravel pads had the reverse (2.7, 30.8). Some species which are native to the desert habitats (e.g., Rumex pictus, Salsola kali, and Carthamus tenuis) were able to invade the North Nile Delta. Conclusions: Habitat of gravel pads is an expressing form about the intense of disturbance in Deltaic Mediterranean coast of Egypt. More of efforts should be carried out to avoid more human disturbances that creating as ruderal habitats which open the gate to invasive species in the flora of North Nile Delta.

A Study on Development of a New Algorithm to Solve Load Flow for Distribution Systems (배전계통조류계산을 위한 새로운 알고리즘에 관한 연구)

  • Moon, Young-Hyun;Yoo, Sung-Young;Choi, Byoung-Kon;Ha, Bock-Nam;Lee, Joong-Ho
    • Proceedings of the KIEE Conference
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    • 1998.07c
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    • pp.918-922
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    • 1998
  • With the development of industry, the qualitical advancement of power is needed. Since it is placed in the end step of power system, the fault at the distribution system causes some users blackout directly. So if the fault occurs, quick restoration is very important subject and, for the reason, induction of the distribution automation system is now being progressed briskly. For the quick restoration of the faulted distribution system, the load shedding of the blackout-area must be followed, and the other problems like the shedded load, faulted voltage and the rest may cause other accident. Accordingly load shedding must be based on the precise calculation technique during the distribution system load flow(dist flow) calculation. In these days because of its superior convergence characteristic the Newton-Raphson method is most widely used. The number of buses in the distribution system amounts to thousands, and if the fault occurs at the distribution system, the speed for the dist flow calculation is to be improved to apply to the On-Line system. However, Newton-Raphson method takes much time relatively because it must calculate the Jacobian matrix and inverse matrix at every iteration, and in the case of huge load, the equation is hard to converge. In this thesis. matrix equation is used to make algebraical expression and then to solve load flow equation and to modify above defects. Then the complex matrix is divided into real part and imaginary part to keep sparcity. As a result time needed for calculation diminished. Application of mentioned algorithm to 302 bus, 700 bus, 1004 bus system led to almost identical result got by Newton-Raphson method and showed constant convergence characteristic. The effect of time reduction showed 88.2%, 86.4%, 85.1% at each case of 302 bus, 700 bus system 86.4%, and 1004 bus system.

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Development of the Controlled Switching Device for a Cirrcuit Breaker

  • Kim, Ik-Mo;Kim, Myung-Chan;Choi, Young-Chan;Ryu, Sung-Sic;Kim, Dong-Hyun
    • 제어로봇시스템학회:학술대회논문집
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    • 2004.08a
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    • pp.558-560
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    • 2004
  • Studies on the controlled switching method have been done to prevent the power system surges which cause the insulation deterioration and electro magnetic compatibility (EMC) problems during closing and opening of a circuit breaker. The controlled switching method controls the closing and tripping time in coincidence with the voltage or current to suppress switching surge. It is used to switch condenser bank, no load transformer, and shunt reactor. In this study, basic concept of the controlled switching is introduced, and also the test is performed to find parameters of the controlled switching in a 24kV vacuum circuit. And then, the control device hardware using TMS320C31 DSP has been designed and manufactured. It has been found that the application of IT technology to a circuit breaker is very effective to depress the switching surge.

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Short Circuit Tests of the Three-Phase DC Reactor Type Fault Current Limiter in Changing of Turns Ratio of Transformers (변압기 권선비의 변화에 따른 3상 DC 리액터형태 한류기의 단락실험)

  • Lee, Eung-Ro;Lee, Chan-Ju;Lee, Seung-Je;Go, Tae-Guk;Hyeon, Ok-Bae
    • The Transactions of the Korean Institute of Electrical Engineers B
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    • v.51 no.6
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    • pp.267-272
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    • 2002
  • This Paper deals with the short circuit tests of the three-Phase DC reactor type fault current limiter (FCL) in changing of turns ratio of transformers. The experiment of this paper is a preliminary step to develop the FCL's faculties for an application to high voltage transmission line. So, superconducting coil was made of Nb-Ti, low temperature superconductor, and the ratings of the power system of experimental circuit are 400V/7A class. A three-phase DC reactor type FCL consists of three transformers, six diodes, one superconducting coil and one cryostat. The important point of experimental analysis is transient period, the operating lagging time of circuit breaker. As the results of the experiment, the values are referred to the limitation rate about 77% and 90% when the turns ratio of transformer was 1:1 and 2:1 respectively.