• 한국식품영양과학회지
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• 제22권2호
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• pp.190-195
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• 1993

Vitis vinifera 적포도의 성숙중 brix, 총당, 유기산, 총산, pH 및 유리아미노산 등의 함량변화를 HPLC 등의 기기를 이용하여 분석하였다. 성숙중 당류는 당이 생성되기 시작한 후 21일 경과할 때까지 급증하였으며, 그 후 증가속도는 둔화되었고, 산류는 이와 반대 현상을 보였다. 성숙된 포도의 성분함량은 품종에 따라 차이가 있었으나, brix 16.5~17.9, 총당 14.76~16.40g/100ml, pH 3.24~3.25 및 총산 0.88~0.928/100ml이었다. 성숙중 brix/acid의 비는 점점 증가하여 성숙된 포도에서 Cabernet sauvignon 18.7, Cabernet franc 20.4 및 Malbec 18.1을 보였다. 성숙된 포도의 F/C의 비율은 1.04~1.06으로 3가지 품종에서 비슷하였고, tartaric/malic acid 비율은 0.83~1.00이었다. 성숙중 유리아미노산은 점점 증가하여 품종별 성숙된 유리아미노산의 함량은 256.8~290.9mg/100ml의 분포였으며, 유리아미노산의 조성에서 3 품종중 가장 많이 함유된 것은 proline이었고, 그 다음으로 arginine, alanine 및 glutamic acid의 순이었으며, 이들 4가지의 아미노산이 총유리아미노산의 73.2~77.2% 범위 였다. 특히 Malbec 품종의 경우 다른 두 품종에 비하여 arginine의 함량이 비교적 많았다.

• Plant Biotechnology Reports
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• 제2권4호
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• pp.233-238
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• 2008

Kadainou R-1, an interspecific hybrid grape derived from red (Vitis ficifolia var. ganebu) and white (V. vinifera cv. Muscat of Alexandria) grapes, accumulates high concentrations of anthocyanin in the berry skin. Hence, the expression of uridine 50 -diphosphate (UDP)-glucose:flavonoid 3-O-glucosyltransferase (UFGT), the key enzyme of the anthocyanin pathway, was examined in the berry skin of Kadainou R-1. As information on gene sequences of V. ficifolia var. ganebu and other wild grape species was unavailable, we performed GeneChip hybridization using biotin-labeled genomic deoxyribonucleic acid (DNA) to investigate how the genomic sequences of V. vinifera varieties and that of V. ficifolia var. ganebu differ. The study showed a lower correlation coefficient between V. vinifera cultivars and V. ficifolia var. ganebu than that among V. vinifera cultivars. The sequences of the UFGT gene derived from both parents of the red and white cultivars were sequenced in Kadainou R1 and revealed that both were expressed irrespective of the fact that it was not expressed in the white grape (male parent).

• 한국식품영양과학회지
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• 제22권2호
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• pp.196-201
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• 1993

Vitis vinifera 적포도주의 화학성분, 고급 알콜, esters 및 휘발성분 등을 측정하기 위하여 HPLC, GC및 GC-MS등의 기기를 이용하였다. 포도주의 저장 숙성중에는 산류의 변화가 많았는데, 특히 tartaric acid가 현저히 감소하였다. Total phenolics와 phenol flavonoid의 함량은 Malbec 품종으로 제조한 포도주가 각각 470mg/L과 245mg/L로서 Cabernet sauvignon (310mg/L, 135mg/L)과 Cabernet franc (425mg/L, 125mg/L) 포도주보다 많았고, nonfalvonoid의 함량은 Cabernet franc 포도주가 300mg/L로서 Malbec (225mg/L)과 Cabernet sauvignon (175mg/L) 포도주보다 많았다. Acetaldehyde 함량은 Cabernet sauvignon (26mg/L)과 Cabernet franc (28mg/L에 비하여 Malter(33mg/L) 포도주에서 많았다. Methyl alcohol, propanol 및 isoamyl alcohol은 다른 두 포도주에 비하여 Cabernet sauvignon 포도주에서 많았고, isobutanol은 Malbec 포도주가 64mg/L이었으며, ethylacetate는 Cabernet franc 포도주가 35mg/L로서 Malbec (28mg/L)과 Cabernet sauvignon (23mg/L) 보다 많았다. 세품종(Cabernet sauvignon, Cabernet franc, 및 Malbec)의 포도주 농축물로부터 GC를 이용하여 87~91여종의 휘발성분을 분리하였고, 그 중 terpine-4-ol을 비롯한 35종을 GC-MS를 사용하여 동정하였다.

• Food Science and Biotechnology
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• 제18권4호
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• pp.1001-1012
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• 2009

A total of 49 phenolic compounds were identified from the aged red wines made from Cabernet Gernischt (Vitis vinifera L. cv.) grapes, a Chinese characteristic variety, including 13 anthocyanins, 4 pryanocyanins, 4 flavan-3-ol monomers, 6 flavan-3-ol polymers, 7 flavonols, 6 hydroxybenzoic acids, 5 hydroxycinnamic acids, 3 stilbenes, and 1 polymeric pigment. Evolution of these compounds was investigated in wines aged 1 to 13 months. Variance analysis showed that the levels of most phenolics existed significant difference in between wines aged 3 and 9 months. Cluster analysis indicated that 2 groups could be distinguished, one corresponding to wines aged 1 to 3 months and the other to wines aged 4 to 13 months. It was thus suggested that there were 2 key-stages for the development of fine wine quality, at the aged 3 and 9 months, respectively. This work would provide some helpful information for quality control in wine production.

• Journal of Plant Biotechnology
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• 제49권4호
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• pp.292-2999
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• 2022

Anthocyanin, an important component in the grape berry skin, strongly affects grape quality. The transcription factors VvMYBA1 and VvMYBA2 (VvMYBA1/2) control anthocyanin biosynthesis. In addition, cultivation and environmental factors, such as light, influence anthocyanin accumulation. The present study aimed to clarify the effect of shading (reduced light condition) on the transcriptomic regulation of anthocyanin biosynthesis using a red-wine grape cultivar, Vitis vinifera 'Pinot Noir', and its white mutant, 'Pinot Blanc', caused by the deletion of the red allele of VvMYBA1/2. The grape berry skins were analyzed for anthocyanin content and global gene transcription accumulation. The microarray data were later validated by quantitative real-time PCR. A decisive influence of VvMYBA1/2 on the expression of an anthocyanin-specific gene, UDP glucose: flavonoid 3-O-glucosyltransferase, was observed as expected. In contrast, upstream genes of the pathway, which are shared by other flavonoids, were also expressed in 'Pinot Blanc', and the mRNA levels of some of these genes decreased in both cultivars on shading. Thus, the involvement of light-sensitive transcription factor(s) other than VvMYBA1/2 was suggested for the expression control of the upstream genes of the anthocyanin biosynthetic pathway. Furthermore, it was suggested that the effects of these factors are different among isogenes.