• Asian Pacific Journal of Cancer Prevention
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• v.18 no.11
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• pp.2919-2923
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• 2017

Objective: Anthocyanins belong to a class of flavonoids, exhibiting antioxidant and anti-inflammatory actions have been reported to have anti-cancer effects. Here, we investigated whether anthocyanins can inhibit cancer cell proliferation, invasion, and angiogenesis in human lung cancer A549 cells, which are critically involved in cancer metastasis. Methods: We used anthocyanins from fruits of Vitis coignetiae Pulliat (AIMs) which has been used in Korean folk medicine for the treatment of inflammatory diseases and cancers. We have performed cell proliferation assays, cell invasion assay, gelatin zymography, wound healing assay and western blotting to examine whether anthocyanins can inhibit cancer cell proliferation, invasion, and angiogenesis in A549 cells. Result: AIMs did not inhibit cancer cell proliferation on A549 cells. Also, AIMs suppressed cancer migration, and invasion by supressing MMP-2 and MMP-9 expression. The Immuno-blotting results also revealed that AIMs suppressed the proteins involved in cancer proliferation (COX-2, C-myc, cyclin D1), migration and invasion (MMP-2, MMP-9), anti-apoptosis (XIAP, and c-IAP2), adhesion and angiogenesis (ICAM-1, VEGF). Conclusion: This study demonstrates that the anthocyanins isolated from fruits of Vitis coignetiae Pulliat inhibit cancer proliferation, cancer migration, and invasion that is involve in cancer-metastasis. This study provides evidence that AIMs might have anti-cancer effects on human lung cancer.

• YAKHAK HOEJI
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• v.54 no.1
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• pp.22-26
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• 2010

We studied the antimicrobial activities of five compounds isolated from the stems of Vitis coignetiae Pulliat and the seeds of Perilla frutescens (L.) Britton. Based on spectroscopic evidence, compounds 1 to 5 were characterized as resveratrol, $\varepsilon$-viniferin, ampelopsin E, apigenin, and luteolin, respectively. The antimicrobial activities against Gram-positive (Staphylococcus aureus) and -negative (Pseudomonas aeruginosa) bacteria and a fungus (Candida albicans) were investigated using the disc diffusion and broth dilution methods. C. albicans was not inhibited by the five compounds. Compounds 2 and 5 had significant anti-microbial activity against S. aureus, and the 50% inhibitory concentration ($IC_{50}$) of compound 2 against S. aureus was 7.2 ${\mu}M$. Compounds 4 and 5 significantly inhibited P. aeruginosa and the minimum inhibitory concentration (MIC) of compounds 2 and 5 was 0.07 and 2.0 ${\mu}M$, respectively. Compounds 2, 4, and 5 had strong anti-microbial activity against S. aureus and P. aeruginosa.

• Toxicological Research
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• v.34 no.1
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• pp.13-21
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• 2018

Anthocyanins are naturally occurring water-soluble polyphenolic pigments in plants that have been shown to protect against cardiovascular diseases, and certain cancers, as well as other chronic human disorders. However, the anti-obesity effects of anthocyanins are not fully understood. In this study, we investigated the effects of anthocyanins isolated from the fruit of Vitis coignetiae Pulliat on the adipogenesis of 3T3-L1 preadipocytes. Our data indicated that anthocyanins attenuated the terminal differentiation of 3T3-L1 preadipocytes, as confirmed by a decrease in the number of lipid droplets, lipid content, and triglyceride production. During this process, anthocyanins effectively enhanced the activation of the AMP-activated protein kinase (AMPK); however, this phenomenon was inhibited by the co-treatment of compound C, an inhibitor of AMPK. Anthocyanins also inhibited the expression of adipogenic transcription factors, including peroxisome proliferator-activated receptor-${\gamma}$, CCAAT/enhancer-binding protein a and b, and sterol regulatory element-binding protein-1c. In addition, anthocyanins were found to potently inhibit the expression of adipocyte-specific genes, including adipocyte fatty acid-binding protein, leptin, and fatty acid synthase. These results indicate that anthocyanins have potent anti-obesity effects due to the inhibition of adipocyte differentiation and adipogenesis, and thus may have applications as a potential source for an anti-obesity functional food agent.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.61-61
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• 2022

섬괴불나무(Lonicera insularis Nakai)는 인동과(Caprifoliaceae) 인동속(Lonicera) 식물로 우리나라 울릉도에 제한적으로 분포한다. 인동과 식물 중 인동(L. japonica Thunb.), 괴불나무[L. maackii (Rupr.) Maxim] 등 다수의 분류군이 약리효능이 입증되었으며, 맛이 뛰어나 차, 술, 음료 등으로 다양하게 활용되고 있다. 그 중 섬괴불나무는 최근 면역자극 및 항비만 활성 등 다양한 약효가 확인되어 약용자원으로서의 잠재적 가치가 큰 것으로 보고된 바 있다. 따라서 본 연구를 통해 특산식물인 섬괴불나무의 적정 자생지를 명확하게 구명하여 보전생물학적 연구, 재배 및 산업화와 같은 현지 외 보전연구를 위한 기초자료로 활용하고자 한다. 연구 결과, 울릉도에서 확인된 섬괴불나무의 주요 분포지는 4개소로 해안도로 일대에 자생하고 있었다. 입지환경은 해발고도 8-20 m, 사면 방위는 남사면, 경사는 15-30°로 완경사지에서 주로 자생하고 있었다. 방형구(10×10 m) 내 개체수는 5-25 개체로 대부분 군락을 이루고 있는 것으로 확인되었다. 4개 조사구에 대한 군집분석을 실시한 결과, 조사구는 곰솔 군집(Pinus thunbergii Parl.)으로 상층의 발달이 거의 없는 전형적인 해안 식생의 구조로 확인되었다. 관목층은 보리장나무(Elaeagnus glabra Thunb.), 송악[Hedera rhombea (Miq.) Bean], 머루(Vitis coignetiae Pulliat ex Planch.) 등이 경쟁관계에 있었으며, 초본층은 왕호장근[Reynoutria sachalinensis (F.Schmidt) Nakai], 갯메꽃[Calystegia soldanella (L.) R.Br], 해국(Aster spathulifolius Maxim.) 등으로 인하여 섬괴불나무는 피압된 것으로 판단된다. 군집의 안전성, 다양성을 나타내는 척도인 종다양성지수는 1.259, 최대종다양성지수는 1.286, 균재도는 0.979, 우점도는 0.021로 확인되었다.