• Journal of Nutrition and Health
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• 제8권1호
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• pp.15-37
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• 1975

I. Subject of the study A study on the nutritive value and utilization of powdered seaweeds. II. Purpose and Importance of the study A. In Korea the shortage of food will be inevitable by the rapidly growing population. It will be very important study to develop a new food from the seaweeds which were not used hitherto for human consumption. B. The several kinds of seaweeds have been used by man in Korea mainly as side-dishes. However, a properly powdered seaweed will enable itself to be a good supplement or mixture to certain cereal flours. C. By adding the powdered seaweed to any cereals which have long been staple foods in this country the two fold benefits; saving of cereals and change of dietary pattern, will be secured. III. Objects and scope of the study A. Objects of the study The objects will come under four items. 1. To develop a powdered seaweed as a new food from the seaweeds which have been not used for human consumption. 2. To evaluate the nutritional quality of the products the analysis for chemical composition and animal feeding experiment will be conducted. 3. Experimental cocking and accepability test will be conducted for the powdered products to evaluate the value as food stuff. 4. Sanitary test and also economical analysis will be conducted for the powdered products. B. Scope of the study 1. Production of seaweed powders Sargassum fulvellum growing in eastern coast and Sargassum patens C.A. in southern coast were used as the material for the powders. These algae, which have been not used for human consumption, were pulverized through the processes of washing, drying, pulverization, etc. 2. Nutritional experiments a. Chemical composition Proximate components (water, protein, fat, cellulose, sugar, ash, salt), minerals (calcium, phosphorus, iron, iodine), vitamins (A, $B_1,\;B_2$ niacin, C) and amino acids were analyzed for the seaweed powders. b. Animal feeding experiment Weaning 160 rats (80 male and 80 female rats) were used as experimental animals, dividing them into 16 groups, 10 rats each group. Each group was fed for 12 weeks on cereal diet (Wheat flour, rice powder, barley powder, potato powder, corn flour) with the supplementary levels of 5%, 10%, 15%, 20% and 30% of the seaweed powder. After the feeding the growth, feed efficiency ratio, protain efficiency ratio and ,organs weights were checked and urine analysis, feces analysis and serum analysis were also conducted. 3. Experimental cooking and acceptability test a. Several basic studies were conducted to find the characteristics of the seaweed powder. b. 17 kinds of Korean dishes and 9 kinds of foreign dishes were prepared with cereal flours (wheat, rice, barley, potato, corn) with the supplementary levels of 5%, 10%, 15%, 20% and 30% of the seaweed powder. c. Acceptability test for the dishes was conducted according to plank's Form. 4. Sanitary test The heavy metals (Cd, Pb, As, Hg) in the seaweed powders were determined. 5. Economical analysis The retail price of the seaweed powder was compared with those of other cereals in the market. And also economical analysis was made from the nutritional point of view, calculating the body weight gained in grams per unit price of each feeding diet. IV. Results of the study and the suggestion for application A. Chemical composition 1. There is no any big difference in proximate components between powders of Sargassum fulvellum in eastern coast and Sargassum patens C.A. in southern coast. Seasonal difference is also not significant. Higher levels of protein, cellulose, ash and salt were found in the powders compared with common cereal foods. 2. The levels of calcium (Ca) and iron (Fe) in the powders were significantly higher than common cereal foods and also rich in iodine (I). Existence of vitamin A and vitamin C in the Powders is different point from cereal foods. Vitamin $B_1\;and\;B_2$ are also relatively rich in the powders.'Vitamin A in ·Sargassum fulvellum is high and the levels of some minerals and vitamins are seemed4 to be some influenced by seasons. 3. In the amino acid composition methionine, isoleucine, Iysine and valine are limiting amino acids. The protein qualities of Sargassum fulvellum and Sargassum patens C.A. are seemed to be .almost same and generally ·good. Seasonal difference in amino acid composition was found. B. Animal feeding experiment 1. The best growth was found at.10% supplemental level of the seaweed Powder and lower growth rate was shown at 30% level. 2. It was shown that 15% supplemental level of the Seaweed powder seems to fulfil, to some extent the mineral requirement of the animals. 3. No any changes were found in organs development except that, in kidney, there found decreasing in weight by increasing the supplemental level of the seaweed powder. 4. There is no any significant changes in nitrogen retention, serum cholesterol, serum calcium and urinary calcium in each supplemental level of the seaweed powder. 5. In animal feeding experiment it was concluded that $5%{\sim}15%$ levels supplementation of the seaweed powder are possible. C. Experimental cooking and acceptability test 1. The seaweed powder showed to be utilized more excellently in foreign cookings than in Korean cookings. Higher supplemental level of seaweed was passible in foreign cookings. 2. Hae-Jo-Kang and Jeon-Byung were more excellent than Song-Pyun, wheat cake, Soo-Je-Bee and wheat noodle. Hae-Je-Kang was excellent in its quality even as high as 5% supplemental level. 3. The higher levels of supplementation were used the more sticky cooking products were obtained. Song-Pyun and wheat cake were palatable and lustrous in 2% supplementation level. 4. In drop cookie the higher levels of supplementation, the more crisp product was obtained, compared with other cookies. 5. Corn cake, thin rice gruel, rice gruel and potato Jeon-Byung were more excellent in their quality than potato Man-Doo and potato noodle. Corn cake, thin rice gruel and rice gruel were excellent even as high as 5% supplementation level. 6. In several cooking Porducts some seaweed-oder was perceived in case of 3% or more levels of supplementation. This may be much diminished by the use of proper condiments. D. Sanitary test It seems that there is no any heavy metals (Cd, Pb, As, Hg) problem in these seaweed Powders in case these Powders are used as supplements to any cereal flours E. Economical analysis The price of the seaweed powder is lower than those of other cereals and that may be more lowered when mass production of the seaweed powder is made in future. The supplement of the seaweed powder to any cereals is also economical with the criterion of animal growth rate. F. It is recommended that these seaweed powders should be developed and used as supplement to any cereal flours or used as other food material. By doing so, both saving of cereals and improvement of individual's nutrition will greatly be achieved. It is also recommended that the feeding experiment for men would be conducted in future.

• Journal of Nutrition and Health
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• 제28권1호
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• pp.33-45
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• 1995

Serum levels of antioxidant vitamins and lipids were determined along with anthropometric measurements in 174 healthy male subjects with mean age of 50.3$\pm$6.8 years from Taegu area in Korea. Body mass index (BMI) and waist/hip(W/H) ratio of the subjects were 23.18$\pm$2.46 and 0.88$\pm$0.04, respectively and their systolic and distolic blood pressures were 127.8$\pm$15.5 and 83.9$\pm$10.8mmHg. Twenty one percent of the subject had BMI over 25. Average seum levels of total cholesterol, LDL- and HDL-cholesterol and triglyceride were 187.7$\pm$34.9, 117.6$\pm$33.5, 41.1$\pm$9.0 and 140.7$\pm$83.6mg/dl, respectively. Sixteen percent of the subject had LDL-chole-sterol over 130mg/dl. Serum level of lipid peroxide measured as thiobarbituric acid reactive substances(TBARS) of the subject was 2.01$\pm$0.77 MDA nmoles/ml and those of $\alpha$-tocopherol, retinol. ascorbic acid and sum of $\alpha$- and $\beta$-carotene were 9.59$\pm$3.11$\mu\textrm{g}$/ml, 1.15$\pm$ 0.38$\mu\textrm{g}$/ml, 10.5$\pm$3.8$\mu\textrm{g}$/ml and 64.6$\pm$43.$\mu\textrm{g}$/dl, respectively. About 14% of the sujects had low vitamin E status of less than 7.0$\mu\textrm{g}$/ml,. while 6% had low vitamin C status of less than 4.0$\mu\textrm{g}$/ml, Serum vitamin E showed positive correlations with total cholesterol and LDL-cholesterol and triglyceride, but no correlation with TBARS. Fatty acids of serum total lipid were composed of 42.9% as saturnted. 19.3% as monounsaturated and 36.7% polyunsaturated fatty acids(PUFA). N-6 and n-3 PUFA were 27.7% and 8.3% of total fatty acids. N-6/n-3 PUFA ratios were negatively correlated both with serum total cholesterol and TBARS.

• 한국식생활문화학회지
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• 제15권4호
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• pp.253-258
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• 2000

국내에서 버려지는 쌀눈활용을 위한 기초연구로 쌀눈의 화삭성분들을 분석하였다. 일반성분은 수분 8.69%, 조지방 21.18%, 조단백질 16.50%, 조섬유 2.95%, 조회분 6.23%, 그리고 당질함량은 44.45%로 나타났다. 유리당은 maltose, glucose, fructose가 있었으며 각각 569.00, 68.80, 50.20mg/100g함유되어 있었다. 아미노산함량은 글루탐산이 1920.09mg/100g, 아르기닌이 15.03mg/100g, 아스파르트산이 1208.7mg/100g, 알라닌이 1001.2mg/100g으로 가장 많이 함유되어 있었으며, 함황아미노산인 시스틴과 메티오닌은 적었다. 무기질 조성은 8종류의 무기성분들이 검출되었으며 칼륨과 인이 각각 가장 많은 1217.80, 1766.22mg/100g이었으며 칼슘, 망감, 나트륨이 각각 36.60, 16.74, 16.22mg/100g으로 많았으며, 아연, 철, 구리도 각각 11.75, 7.99, 1.16mg/100g이 들어 이었다. 비타민함량은 5가지의 수용성 비타민과 지용성비타민이 토코페롤이 함유되어 있었으며, 비타민 E가 11.96mg/100g으로 가장 많았고 $B_1$과 $B_2$는 각각 5.69mg/100g, 0.45mg/100g였으며 비타민 C는 1.66mg/100g 그리고 나이아신과 비타민 $B_6$는 각각 2.96mg/100g, 0.69mg/100g였다. 지방산은 6가지 종류의 지방산이 함유되어 있었으며, 주지방산은 linoleic acid와 oleic acid로 전체지방산의 약 64%를 차지하고 있다. 또한 모든 유지류에 널리 분포되어 있는 palmitic acid가 22.2%였으며, arachidic acid도 3.1%였다. 16종의 flavonoid와 9종의 phenolic acid를 screening한 결과 쌀눈에서는 검출되지 않았다.

• 한국식품영양학회지
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• 제23권4호
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• pp.516-525
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• 2010

아마인의 유용성분에 대해서 알아보고 가열과정을 통한 성분변화 및 다른 종유작물과 유지의 물리화학적 성분과 비교, 평가하였다. 조지방은 37~43%, 탄수화물은 30~35%, 조단백질은 18~23%, 조회분은 3~4% 그리고 수분 함량은 0.2~6.8%의 함량을 나타내었다. 식이섬유 함량은 생아마인보다 가열 처리한 볶음 아마인에서 SDF의 함량이 증가한 것을 알 수 있었다. 하지만 상대적으로 IDF의 함량도 감소하기 때문에 TDF의 함량은 큰 차이를 보이지 않았다. 그리고 총 식이섬유 함량은 28~31%로 높은 함량을 보였다. 또한 아마인의 주요 유용성분을 살펴본 결과, 비타민은 ${\gamma}$-tocopherol(E), 아미노산은 agrginine, aspartic acid, glutamic acid가 높은 함량을 보였다. 이들은 생아마인보다 볶음 아마인에서 낮은 함량을 나타내었다. 아마인의 무기물 함량을 분석한 결과, 칼슘, 칼륨, 마그네슘, 인이 주요 구성성분이었다. 물리화학적인 특징으로 요오드가, 비누화가, 산가를 분석하였으며, 브라운 아마인유는 각각 204.1 g/100 g, 193.6 mg/g, 1.59 mg/g을 나타내었으며, 골드 아마인유는 각각 203.0 g/100 g, 189.9 mg/g, 2.35 mg/g으로 조사되었다. 아마인유의 지방산 함량에서는 전체적인 구성 비율을 볼 때 ${\alpha}$-linolenic acid(ALA, C18:3n-3)가 약 55.5~56.1% 로 가장 높은 함량을 차지하였다. 그리고 브라운보다는 골드 아마인유의 ${\alpha}$-linolenic acid의 함량이 약간 높았다. 높은 ${\alpha}$-linolenic acid 함량은 아마인유가 오메가-3 지방산이 강화된 식품 개발에 좋은 자원이 될 것으로 사료된다. 또한 다가 불포화지방산 함량이 약 71%로 함유되어 있으며, ${\alpha}$-linolenic acid 등 필수지방산의 함량이 높아 식품의 영양학적인 의의가 크며, 양질의 식용유로 사용 가능할 것이다.

• 한국식품영양과학회지
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• 제35권6호
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• pp.780-785
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• 2006

무청별 무청의 성분분석과 반응표면 분석법을 이용하여 건조조건에 따른 무청 고유의 색도, 칼슘, 철분, 비타민 등의 성분을 조사하였다 일반성분의 경우 품종이 다른 미농단백무(RL5)를 제외하고는 품종 간에 회분, 단백질, 지방, 칼슘 및 철분함량에 유의적인 차이가 없었고, 건물량으로 환산한 비타민류, 클로로필 함량, 색도에는 품종 간 유의적인 차이가 있었다. 한편 중심합성 실험계획에 따라 건조한 미농단백무 무청의 품질특성을 조사한 결과 수분함량, 클로로필 함량 및 색도차이를 나타내는 ${\Delta}E$ 값은 건조온도와 시간에 따라 유의적인 차이가 있었고, 칼슘, 철분함량은 각각 $31.41{\sim}35.80\;mg,\;0.21{\sim}0.29\;mg$으로 시료 간 유의성이 없었다. 무청의 건조조건을 독립변수로 하고, 시료 간 유의적인 차이를 보였던 종속변수인 수분, 클로로필 및 색도 값의 상관관계를 나타내는 $R^2$값이 0.97 이상으로 높았다. 또 회귀분석한 모델식중 반응표면 분석법으로 도시한 결과 총 클로로필 함량은 독립변수인 건조시간보다는 건조온도의 영향이 더 컸으며 무청의 고유 초록색을 유지하면서도 수분 감소효과를 얻기 위한 최적조건은 약 $70^{\circ}C$에서 $5{\sim}6$시간 정도로 예측할 수 있었다.

• Journal of Nutrition and Health
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• 제51권5호
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• pp.400-413
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• 2018

본 연구에서는 2007 ~ 2016년 국민건강영양조사의 검진 조사와 식품섭취조사를 완료한 만 19세 이상의 성인 여성 30,425명을 대상으로 항산화비타민 섭취량과 비만 및 복부비만의 연관성을 분석하였다. 체질량지수가 $25kg/m^2$ 이상이거나 허리둘레가 85 cm 이상인 대상자를 각각 비만과 복부비만으로 판정하였고, 개인별 일일 항산화비타민 섭취량은 24시간 회상법을 이용한 식사자료와 한국인 상용식품의 항산화비타민 함량 데이터베이스를 연계하여 추정하였다. 항산화비타민에는 카로티노이드 6종, 레티놀, 비타민 A (레티놀 활성 당량), 비타민 C, 토코페롤 4종, 비타민 E (${\alpha}$-토코페롤 당량)이 포함되었다. 정상군과 비만군의 열량 1,000 kcal당 일일 항산화비타민 섭취량의 평균을 비교한 결과, 비만군은 ${\alpha}$-카로틴, 레티놀, 비타민 E, ${\alpha}$-토코페롤, ${\gamma}$-토코페롤을 정상군보다 적게 섭취한 것으로 나타났다. 항산화비타민 섭취수준에 따라 대상자를 삼분위로 구분했을 때, 라이코펜을 가장 많이 섭취하는 군은 가장 적게 섭취하는 군에 비해 비만 (OR = 0.89, 95% CI: 0.83-0.96)과 복부비만 (OR = 0.88, 95% CI: 0.81-0.95) 유병률이 유의하게 낮았다. 총 카로티노이드, ${\alpha}$-카로틴, 비타민 A, ${\gamma}$-토코페롤 섭취량 또한 복부비만의 오즈비와 음의 연관성을 나타냈다. 식품군별 항산화비타민 섭취량을 비교했을 때, 비만군은 정상군에 비해 난류, 우유류, 조미료류, 곡류를 통한 항산화비타민 섭취량이 적었다. 추후 전향적 설계를 이용한 후속연구를 수행하여 항산화비타민 섭취량과 비만 간의 인과성을 확인할 필요가 있을 것으로 사료된다.

• Journal of Nutrition and Health
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• 제51권5호
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• pp.386-399
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• 2018

본 연구는 고혈압 및 당뇨병 치료 중인 환자 41명을 대상으로 한식 섭취가 12주간의 경과 과정에서 4차례의 방문(0주, 4주, 8주, 12주)을 통해 측정한 혈청 GGT, 혈압 및 glycemic control data, 심혈관계 위험지표 및 식이섭취의 변화를 비교 평가한 결과는 다음과 같았다. 1. 연구대상자는 평균 연령은 $61.8{\pm}1.9$세로 한식군 (21명)의 경우 건강한 한식을 1일 3끼씩 12주간 제공된 식사를 섭취하였고, 대조군 (당뇨병 식이 가이드라인에 따른 관리교육을 받은 자) 20명은 평소 섭취하던 당뇨조절 식사를 그대로 유지하게 하였다. 2. 수축기혈압과 이완기혈압은 두군 간 유의한 차이를 확인할 수 없었으나 맥박수는 한식군에서 대조군에 비해 유의적으로 감소하였다 (p = 0.007). 3. 당화혈색소 (HbA1c)는 한식군에서 연구 참여 전과 후 각각 $6.8{\pm}.2%$, $6.1{\pm}.2%$로 감소하고 대조군은 각각 $6.8{\pm}.2%$, $6.5{\pm}.2%$로 감소되어 한식군이 대조군에 비해 유의적으로 감소폭이 컸다 (p = 0.004). 4. 혈청 GGT는 한식군에서 연구 참여 전과 후 각각 $28.8{\pm}4.5IU/L$, $19.8{\pm}3.2IU/L$로 감소하고 대조군은 각각 $30.0{\pm}4.1IU/L$, $33.9{\pm}5.0IU/L$로 증가되어 한식군이 대조군에 비해 유의적으로 감소하였다 (p < 0.001). 5. 총콜레스테롤, LDL 콜레스테롤 및 중성지방은 한식군에서 대조군에 비해 감소한 경향이었으나 두군 간 유의적인 차이가 없었으며 free fatty acid는 한식군에서 대조군 보다 유의적으로 감소하였다 (p = 0.002). 6. 신체계측 지표인 체중 (p = 0.002), 체질량지수 (p = 0.002), 체지방량 (p < 0.001), 체지방율 (p < 0.001)은 연구 참여 전에 비해 연구 참여 12주 후에 한식군에서 대조군에 비해 유의적으로 감소하였다. 7. 치료약물의 변화량은 두군 간 유의한 차이는 없었으나 한식군에서 대조군보다 많은 수의 환자가 실제로 고혈압, 당뇨병 및 고지혈증 치료 약물을 감량할 수 있었다. 8. 한식군은 연구 참여 동안에 전곡류의 밥, 채소류인 나물류, 김치 및 전통 콩발효식품 등의 섭취량은 대조군에 비해 유의하게 증가하였다 (p < 0.001). 9. 한식군은 동물성식품에서 유래한 동물성 단백질 (p < 0.001), 지질 (p < 0.001) 및 콜레스테롤 (p = 0.034)의 섭취량은 대조군에 비해 유의적으로 감소한 반면, 총칼로리(p < 0.001), 엽산 (p < 0.001), 식이섬유 (p < 0.001), 나트륨(p < 0.001), 칼륨 (p < 0.001), 비타민 A, C, E (p < 0.001) 및 비타민 B류 (p < 0.001)의 섭취는 유의적으로 증가하였다. 이상의 결과를 종합해 보면, 적극적인 전곡류로 만든 밥을 중심으로 한 한식 섭취는 당뇨병 및 고혈압 환자에서 대사성질환 및 심혈관계질환 위험인자인 혈청 GGT, 맥박수, 혈당조절 지표 및 비만지표 개선에 긍정적인 영향을 주었다.

• 생명과학회지
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• 제29권3호
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• pp.354-361
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• 2019

고추(Capsicum annuum L.)는 한국 전통 음식의 중요한 채소 중 하나이며, 비타민 A, 비타민 C, 비타민 E, 폴리페놀 및 플라보노이드를 많이 함유하고 있다. 또한 고추는 높은 항산화능을 가지고 있으며, 비만, 당뇨병, 고혈압, 소화 장애, 스트레스 및 노화를 예방하는데 효과적이라고 알려져 있다. 본 연구에서는 발효 홍고추 및 발효하지 않은 홍고추의 항비만 및 당뇨병 효과를 조사하였다. 8 주 동안 45% HFD를 공급하여 비만 유도 시킨 C57BL / 6N 마우스는 HFD 사료와2.5% NRM, 1.25% FRM, 2.5% FRM을 함유한 HFD 사료를 8 주간 투여하였다. 식이섭취 시킨 후 7주 경과 후 경구당 부하검사(OGTT)를 진행하였고 8주 경과 후 체중, 간, 부고환 지방량, 혈청 인슐린 수치 및 HOMA-IR을 측정하고 지질 함량 검사를 시행하였다. 이 연구의 결과는 2.5% FRM이 HFD와 2.5% NRM보다 체중, 조직 중량, 지질 함량, 혈청 인슐린 수치 및 HOMA-IR을 감소시켰다. 이 결과를 통해 발효 홍고추는 항비만 및 항당뇨에 효과가 있는 것을 확인하였다. 또한, 본 연구는 발효 홍고추를 이용한 건강기능식품 및 기능성 생리활성증대 소재의 기초자료로 활용할 수 있을 것이다.

• 대한지역사회영양학회지
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• 제10권1호
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• pp.101-110
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• 2005

This survey was carried out to obtain the information concerning nutritional status, including factors of food habits and nutrient intake of the long-lived elderly men and women living in Ganghwa-gun. In order to assess the quality of dietary intake among the elderly, a survey was conducted during December 2003 of 103 subjects who were over 85 years of age. Dietary nutrient intake data were obtained through the 24 hr recall method. Chi-square test and t-test were the main data analysis method. Their dietary habits such as three meals a day and a regular meal time have shown that they have generally good eating habits. Average daily calorie intake ($\%$ RDA) was 1233.2 kcal ($68.8\%$) for male and 1215.8 kcal ($75.8\%$) for female which were lower than the Recommended Dietary Allowances (RDA) for Koreans. Energy intake of females got closer to RDA than that of male. Protein intake was 49.3 g for male and 46.9 g for female (which was $82.3\%$ RDA for male and $85.1\%$ RDA for female) for elderly person, the proportion of animal protein to total protein intake were $45.2\%$ for male and $39.0\%$ for female. Average CPF ratio of energy intake for both male and female were 68.7 : 16.1 : 15.2 and 69.6 : 15.4 : 15.0. SFA : MUFA : PUFA ratio of the subject was 0.78 : 1.03 : 1.00 for male and 0.64 : 0.92 : 1.00 for female. Calcium intakes for both males and females were 321.3 mg and 377.2 mg. Vitamin A was the nutrient found to be least sufficient. Mean daily intakes of most of the vitamins and minerals for both males and females were lower than RDA except vitamin C and Zn for female, especially $\%$ RDAs of vitamin A, Ca for male and vitamin A for females were less than $50\%$ of RDA. In conclusion, long-lived elderly in Ganghwa areas did not consume enough nutrients quantitatively as well as qualitatively, especially Ca, Fe, vitamin A, vitamin $B_2$ and vitamin E. These results suggest that nutritional guidelines for older Koreans should focus on the maintenance of adequate energy intake. In addition, selection of foods with high protein and calcium, such as dairy food, should be emphasized, particularly in the long-lived elderly.

• 대한지역사회영양학회지
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• 제12권4호
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• pp.396-404
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• 2007

Increased oxidative stress contributes to the progression of atherosclerosis. We measured serum antioxidant mineral concentrations, capacities of serum antioxidant enzymes and fasting lipid profile in 97 male patients with coronary artery disease (CAD) and 21 male controls. Nutrient intake was assessed by the semi-quantitative food frequency method. CAD patients were divided into single-vessel disease (SVD, n=66) and multi-vessel disease (MVD, n = 31) groups on the coronary angiography. The ratio of serum LDL- to HDL-cholesterol elevated with an increasing number of diseased vessels compared to the control (control < SVD < MVD, p < 0.05). Patients with SVD and MVD had higher levels of serum lipoprotein (a) than the control (p < 0.05). The mean intake of carbohydrate, protein and cholesterol was higher in MVD patients and the intakes of vitamins C and E were lower in MVD and SVD patients than in the control (p < 0.05). Serum copper (Cu) and zinc (Zn) levels were higher in MVD and SVD patients than in the control (Cu: control $75.8{\pm}5.07$, SVD $99.2{\pm}2.90$, MVD $100.1{\pm}2.32{\mu}g/dL$, p<0.01; Zn: $76.8{\pm}5.36$, $119.0{\pm}5.95$, $129.1{\pm}2.70{\mu}g/dL$, p < 0.01). And the ratio of Zn to Cu was higher in SVD and MVD patients than in the control (control $0.78{\pm}0.06$, SVD $0.88{\pm}0.05$, MVD $0.99{\pm}0.04$, P < 0.05). The activity of glutathione peroxidase (GSH-Px) was lower in MVD than in SVD and the control (control $35.13{\pm}1.34$, SVD $35.30{\pm}1.01$, MVD $31.00{\pm}1.04 U/mg$ protein, p < 0.05). The ratio of the activities of superoxide dismutase (SOD) to GSH-Px was higher in MVD than in control and SVD (p < 0.05). In groups with CAD, serum Cu and Zn concentrations and their ratio were changed compared to the control. GSH-Px activity was decreased and the ratio of SOD to GSH-Px was increased in the patients with MVD. The balances between the activities of SOD and GSH-Px should also be considered a risk factor in CAD patients.