• 한국응용곤충학회지
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• 제59권3호
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• pp.265-275
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• 2020

본 연구에서는 양봉산물로서의 가치 평가와 제품다양화를 위한 기초자료 제공을 위해 수벌번데기(16 ~ 20일령)의 영양성분분석 및 이를 이용한 추출물을 제조하고 생리활성 효과를 검정하였다. 수벌번데기 동결건조 분말의 일반성분 분석 결과 수분 1.69 ± 0.07%, 조단백 48.52 ± 0.20%, 조지방 23.41 ± 0.14%, 조회분 4.05 ± 0.02% 였고, vitamin C 14.92 ± 0.52 mg/100 g, vitamin E 6.06 ± 0.11 mg α-TE/100 g 이었으며, 무기성분은 K과 P의 함량(mg/100g)이 각각 1,349.13 ± 34.57 및 1,323.55 ± 43.85로 가장 높게 함유되어 있고, Ca과 Fe의 함량은 각각 55.43 ± 1.51 및 5.49 ± 0.19가 함유된 것으로 분석되었다. 수벌번데기 동결건조분말 중에 함유된 지방산은 포화지방산(g/100g total fatty acids)이 약 59.62, 불포화지방산이 약 40.38을 차지하였고, 고급지방산인 palmitic acid (C16:0)가 35.49 ± 0.08, 그리고 oleic acid (C18:1, n-9)가 35.91 ± 0.22로 다량 함유되어 있었다. 구성아미노산(g/100 g)은 총 38.99 ± 2.63이 함유된 것으로 분석되었고, 유리아미노산(mg/100 g)은 총 5,129.04 였으며, 이중 proline이 1,257.68, glutamic acid가 759.12로 가장 높았다. 수벌번데기 추출물의 DPPH radical-scavenging 활성(㎍/ml)은 증류수추출 시 0.8, 50% EtOH 추출 시 3.2, 70% EtOH 추출 시 6.4, 그리고 EtOH 추출 시 32 농도에서 약 90% 이상이었다. 이와 같은 결과는 항산화능에 기여하는 주요 화합물이 극성계 화합물임으로 추정되었으며, 저분자단백질 또는 유리형 아미노산일 가능성이 높은 것으로 추측되었다. 이상의 결과와 같이 수벌번데기는 우수한 식품원료로의 사용과 새로운 기능성 소재로의 활용을 기대할 수 있을 것으로 사료된다.

• 생물환경조절학회지
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• 제10권3호
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• pp.181-186
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• 2001

시설에서 고추, 오이, 토마토 등 과채류 생육기간에는 초관 하부에 광이 부족하게 된다. 본 연구는 플라스틱필름 시설에서 풋고추를 재배할 때 산광필름이 생육, 초관, 개화 및 착과에 미치는 영향을 구명하고자 실시하였다. 시설내 환경을 측정한 결과, 일사량의 투과율은 직조필름과 복층필름에서 일반필름의 90%, 75% 수준이었다. 또한 광합성유효광의 투광율은 직조필름과 복층필름에서 일반필름의 96%, 81% 수준이었다. 그러나 직조필름, 복층필름 및 일반필름의 산란광율은 각각 46%, 31%, 9%이었다. 일반필름으로 피복된 시설에서 정식후 48일 측정한 초장은 직조필름과 복층필름으로 피복된 시설보다 각각 13.9cm, 6.5cm 큰 96.9%cm이었다. 그리고 제3절의 길이는 직조필름과 복층필름으로 피복된 시설보다 각각 5.7cm, 2.5cm 짧은 8.6cm이었다. 또한 직조필름으로 피복된 시설에서 제1분지각은 복층필름과 일반필름으로 피복된 시설보다 2.3$^{\circ}$큰 61.0이었고, 붙임과율은 1.2%, 3.6% 낮은 2.1%이었다. 그러나 피복재간의 풋고추 분지수와 비타민C의 함량은 차이가 없었다.

• 한국균학회지
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• 제11권2호
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• pp.79-84
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• 1983

Armillariella mellea의 2차균사를 potato dextrose medium, yeast extract medium, malt extract medium Hamada medium, 등에 배양해서 생리 생태적 성질을 연구했다. 그 결과를 요약하면 아래와 같다. 1. 사용했던 4종의 배치 중에서 malt extract medium에서 가장 잘 자랐고, 그 다음 yeast extract medium, potato dextrose medium 그리고 Hamada medium 순으로 생육율이 높였다. 2. $27^{\circ}C$에서 가장 잘 자랐고, $36^{\circ}C$에서는 집락의 지름이 $27^{\circ}C$와 비슷하나 균사의 밀도가 매우 낮았다. 3. pH6에서 가장 높은 생육율을 나타내었다. 4. 식물 생장 호르몬을 첨가한 경우에는 Heteroauxin의 5ppm 농도에서 대조군보다 26.2% 증가로 가장 많은 생육 증량을 보이고, 다음으로 gibberellin 100ppm, Tomatoton 10,000배액, 그리고 Adoton 5,000배액의 순서였다. 식물 생장 호르몬을 적당 농도로 사용하면 생장 증량 효과를 얻을 수 있으나, 과량 사용할 경우, 오히려 생장 억제 효과를 나타내었다. 5. Vitamin 첨가경우에 있어 200ppm 농도의 균일한 조건하(下)에서 Vit.A 첨가군에서 대조군보다 18.2%의 가장 많은 생육 증량을 보였고, $Vit{\cdot}D_2\;Vit{\cdot}E,\;Vit{\cdot}B_1\;folic\;accid\;Vit{\cdot}C$ 그리고 $Vit{\cdot}B_2$의 순으로 그 증가가 나타나 지용성 비타민 첨가 배지에서 상당한 생육율을 나타내었다.

• 대한지역사회영양학회지
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• 제7권6호
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• pp.844-851
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• 2002

Smoking can increase oxidative stress and thereby change the antioxidant defense system in the body. To investigate the relationship between male adolescent smoking and antioxidant status, we surveyed the eating habits and dietary intake of 82 smokers and 44 nonsmokers recruited from a male technical high school. In addition, antioxidant enzyme activity and lipid peroxide values were determined in both the plasma and the erythrocytes. Although the frequency of food intake was not significantly different, most nutrient intake was unexpectedly higher in smokers than in nonsmokers. In comparison with the Korean RDA, especially the average intake of Ca, Fe and vitamin $B_2$ didn t reach 75% of the Korean RDA in either smokers or nonsmokers. An analysis of antioxidant enzyme activity showed that plasma catalase. superoxide dismutase (SOD), glutathione peroxidase (GSH-px), erythrocyte catalase and GSH-px activities showed no significant difference between smokers and nonsmokers. However, the erythrocyte SOD activity of smokers (1.57 unit/mgHb) was significantly lower than that of nonsmokers (2.00 unit/mg Hb). In addition, the plasma ceruloplasmin concentration of smokers (28.68 mg/$d\ell$) was significantly higher than that of nonsmokers (26.30 mg/$d\ell$), whereas the specific ceruloplasmin ferroxidase activity of smokers (0.31 unit/mg) was lower than that of nonsmokers (0.35 unit/mg). The plasma and erythrocyte thlobarbituric acid reactive substance (TBARS) of smokers (2.57 $\mu$mol/L, 0.32 $\mu$mol/gHb) were also significantly higher than those of nonsmokers (2.25 $\mu$mol/L, 0.27 $\mu$mol/gHb). The overall data indicate that adolescent smoking might decrease the antioxidant capacity of the body, in part, by lowering the erythrocyte SOD activity and the specific ceruloplasmin ferroxidase activity.

• 생명과학회지
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• 제22권12호
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• pp.1712-1717
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• 2012

Low density lipoprotein (LDL)의 산화는 동맥경화의 유발과 진행에 중요한 역할을 하는 것으로 알려져 있다. 본 연구는 숙성 마늘 유래 유황화합물인 S-allylmercaptocysteine의 항산화 효과를 실험하였다. S-allylmercaptocysteine의 항산화 효과는 $Cu^{2+}$ 유도 LDL에 대하여 thiobarturic acid substance (TBARS)와 전기영동 이동거리, 공액 이중결합으로 측정하였다. 사람 LDL을 $Cu^{2+}$ 유도로 산화시킬 때 S-allylmercaptocysteine은 용량 의존형으로 나타났으며, 농도가 $20{\mu}g/ml$ 일 때 거의 완전한 억제 효과를 나타내었다. S-allylmercaptocysteine에 대한 전기영동 이동거리로 $20{\mu}g/ml$ 일 때 가장 낮았으며 공액 2중결합 형성도 $20{\mu}g/ml$ 일 때 거의 억제되었다. 한편 S-allylmercaptocysteine을 항산화제인 $d{\ell}-{\alpha}$-tocopherol과 LDL에 대한 항산화력을 비교한 결과 같은 농도에서 S-allylmercaptocysteine이 약간 높았다. 따라서 S-allylmercaptocysteine이 동맥경화의 발병과 진행을 예방하는 역할을 할 것으로 생각된다.

• 한국환경성돌연변이발암원학회지
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• 제24권2호
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• pp.51-66
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• 2004

Retinoids, better known as vitamin A, have been reported to inhibit the growth of several breast cancer cell lines in culture and to reduce breast tumor growth in animal models. Furthermore, retinoids can augment the action of other breast cancer cell growth inhibitors both in vitro and in vivo. Clinically, interest has increased in the potential use of retinoids for the prevention and treatment of human breast cancer. We have examine the effect of all-trans retinoic acid(tRA) and 9-cis retinoic acid(9-cis RA) on human breast cancer cell(MCF-10A, T47-D, MCF-7) proliferation using MTT assay and cell cycle analysis(FACS). Overexpression of cyclin D1 protein is observed in the majority of breast cancers, suggesting that dysregulated expression of cyclin D1 might be a critical event in breast cancer carcinogenesis. We investigated whether tRA and 9-cis RA might affect expression of cyclin D1 on human breast cancer cells(MCF-10A, T47-D, MCF-7) using RT-PCR and west-ern bolt. In MCF-10A cells, either tRA or 9-cis RA treatment did not affect the cell proliferation. In T47-D cells and MCF-7 cells, either tRA or 9-cis RA treatment showed the inhibition of the cell proliferation over control cells and also inhibit the estrogen stimulated cell proliferation when it was given together with estrogen. The effect of retinoids was dose- and time- dependent. T47-D cells treated with 1.0 $\muM$ tRA undergo G0/G1-phase arrest by Day 5. MCF-7 cells treated with 1.0 $\muM$ tRA undergo S-phase arrest by Day 5. All-trans retinoic acid(tRA) and 9-cis retinoic acid(9-cis RA) inhibited the cyelin D1 mRNA and protein expression levels of human MCF-7 and T47-D breast carcinoma cells in vitro. The data indicate that retinoids can reduce cyclin D1 expression levels in a variety of breast cell lines in vitro and result in inhibition of cell proliferation. tRA-mediated growth inhibition and cyclin D1 expression inhibition is more potent than 9-cis RA mediated that. tRA-mediated inhibition effect is more potent on T47-D cells than on MCF-7 cells. Our data suggest that retinoids activity is different according to property of cell lines. Future chemoprevention of breast cancer studies using retinoids will be necessary to determine the mechanism of the retinoids-mediated growth inhibition.

• Journal of Nutrition and Health
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• 제31권1호
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• pp.36-45
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• 1998

Dietary and other factors affecting bone density of 32 Korean healthy college women aged 19-23 years were assessed. Data for food and nutrient intake was obtained by a semiquantitiative food frequency questionnaire. Serum samples were anlayzed for total Ca, P, Ca++, PTH, calcitonin and 25-hydroxycholecalciferol , (25-OH-Vit D3) and BMDs of lumbar spine(L2-L4), femoral neck(FN), ward's triangle (WT) and trochanter(TR) were measured by an XR-series X-ray bone densitometer. Relationships between the factors and BMDs were analyzed by stepwise multiple regression analysis and Pearson's correlation coefficient(r). The results are summarized as follows. Mean daily intake of energy(86.1%), Ca(74.3%), vitamin A (53.75), Fe(49.75) and vitamin B$_2$(86.6%) were lower while other notrients incuding P(126%) were higher than the Korean RDA. The BMDs of lumbar spines and femurs ranged from 0.73g/$\textrm{cm}^2$ to 1.23g/$\textrm{cm}^2$and 0.48g/$\textrm{cm}^2$ to 1.04g/$\textrm{cm}^2$, respectively. Both protein and P intakes were inversely associated with serum total Ca. Furthermore, Ca intake as well as Ca/P ratio (Ca/P) were inversely associated with serum ionized Ca(Ca++) concentration. The intakes of protein P and Ca , however, were not significantly associated with the BMDs measured in this study. There were little association between BMDs and alcoholic beverage or caffeine consumption. The only significant association detected was a positive relationship between caffeine consumption and BMD of WT. It seemed to be noticeable that BMDs of L2-L$_4$, FN and WT were significantly inversely associated with serum P concentration. However, there was no significant association between BMDs and the levels of total Ca to Ca++ in serum. Body weight was positively associated with BMD of lumbar spine and BMI was also positively associated with BMDs of FN and WT. The subjects who had an early menarche appeared to have higher BMDs than those who had had a late mearche. According to stepwise multiple regression analysis. Menarche and BMI were stronger determinants of BMDs in the young women than was diet. P intake appeared to be a more potent dietary determinant than Ca intake. The three factors, menarche, BMI , and P intake , additionally accounted for 24% and 378% of the variance in BMDs of FN and L$_2$-L$_4$, respectively. Further investigation is necessary to determine the factors needed to increase serum P level which negatively affects. BMD in young Korean college women.

• 한국식품과학회지
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• 제48권1호
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• pp.86-91
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• 2016

백년초를 80% (v/v) 에탄올-물 혼합용액으로 추출하여 총페놀 함량, 총플라보노이드 함량, 산화방지능, 신경세포 보호효과, AChE 및 BChE 활성저해능을 평가하였다. 백년초 100 g FW당 총페놀 함량은 409.9 mg GAE, 총플라보노이드 함량은 72.2 mg CE이었다. 백년초(100 g FW)의 산화방지능은 ABTS, DPPH, ORAC법에서 각각 381.2, 298.2, 3,219.9 mg VCE였다. 백년초 추출물은 $H_2O_2$의 산화 스트레스로부터 세포 내 산화 스트레스를 감소시켜 신경세포 PC-12의 생존율을 농도의존적으로 증가시켰다. 또한, 백년초 추출물은 AChE 및 BChE 활성을 저해하였다. 본 연구 결과로부터 백년초의 세포 내 산화 스트레스 감소에 의한 신경 세포 보호능과 AChE 및 BChE 저해능이 확인 되었으며, 이러한 결과를 통해 향후 백년초를 이용한 기능성 제품 및 여러 가공품 개발에 활용이 가능할 것으로 기대한다.

• 한국응용과학기술학회지
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• 제10권2호
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• pp.19-27
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• 1993

Levels of sterols including ${\Delta}^7$-dehydrogenase isolated from the tissues of marine animal products (20 species) were determined on 1.5% OV-17 columm of gas-liquid chromatography. The composition showed that the mussels and clams contained various sterols in their tissues : cholesterol, brassicasterol. 24-methylenecholesterol with some minor components such as 22-trans-norcholesta-5,22-dien-3${\beta}$-ol, 22-cis-dehydrocholesterol, 22-trans-dehydrocholesterol, desmosterol, 7-dehydrocholesterol, campesterol, stigmasterol, ${\beta}$-sitosterol, isofucosterol, and 7-dehydrocholesterol which could be converted into vitamin $D_3$ in the skin tissue of animal was present in the muscle of oyster, Crassostrea gigas. On the other hand, the others including gastropoda were predominantly composed of cholesterol. The minor sterols such as 24-methylenecholesterol, stigmasterol and ${\beta}$-sitosterol in the fish intestines are supposed to be derived from dietary plankton. Cholesterol ${\Delta}^7$-dehydrogenase which could convert cholesterol into ${\Delta}^7$-dehydrogenase was present in the pickles of Tricurus haumela intestine.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권2호
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• pp.75-88
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• 2001

In this paper, the microbial production of riboflavin is reviewed and includes descriptions of riboflavin overproducers, and the biosynthesis and details of the key-enzyme genes related to riboflavin. There kinds of riboflavin overproducers are known; Bacillus subtilis and Candida famate utilize glucose as a carbon source, but the fungus Ashbya gossypii requires plant oil as its sole carbon source. The starting material in ribofalvin biosynthesis is guanosine triphospate (GTP), which is converted to riboflavin through six enzymatic reactions. Though Bacillus subtilis, Candida famate, and Ashbya gossypii operate via different pathways until GTP, they follow the same pathway from GTP to riboflavin. From the metabolic viewpoint, with respect to improved riboflavin production, the supplementation of GTP, aprocess-limiting precursor must be considered. The GTP fluxes originate from three sources, serine, threonine and glyoxylate cycles. The development of pathways to strengthen GTP supplementation using biotechnological techniques remains an issue fro future research.