• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.4
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• pp.341-352
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• 2021

Acerola is an excellent ingredient because of its high natural vitamin C content, but it is difficult to stabilize and has hardly been studied as a cosmetic material. Therefore, this study developed a mixed liposome preparation for stabilizing acerola extract. As a safety test, the skin irritation test was evaluated by BCOP assay and HET-CAM assay. We evaluated the inhibition of tyrosinase activity, the whitening effect of melanin production, and the wrinkle effect of prochloragentype-I C-peptide production, and confirmed the possibility of functional cosmetics. In addition, a cream of liposomes containing acerola extract mixture was developed to evaluate the clinical studies of skin wrinkles and whitening. BCOP assay, HET-CAM assay and human skin primary irritation test results of liposomes containing acerola extract mixture showed no irritation and were safe from skin and eye. The result of tyrosinase activity by 75.8% at 1,000 ㎍/mL. As a result of the melanogenesis inhibition test, liposome with acerola extract showed the melanin content by 46.2% at 1,000 ㎍/mL that does not effect the viability of the B16F10 cell line. The result of collagen production test using ELISA kit, liposomes containing acerola extract mixture showed collagen synthesis ability by 152.1% at 1,000 ㎍/mL that does not affect the viability of the HS68 cell line. But it did not showed any inhibition of collagenase (MMP-1) activity at all concentrations in the MMP-1 activity inhibition test in the HS68 cell line. We performed clinical studies for the whitening and skin-wrinkle activity of cream containing acerola extract mixes liposome, was showed that the melanin contents and wrinkle was statistically significant reduction. These results suggest that liposomes containing acerola extract mixture have safe natural material, and skin wrinkle, whitening effects allowing their application in cosmetics as a natural product.

• Journal of Nutrition and Health
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• v.50 no.3
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• pp.270-283
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• 2017

Purpose: This study was conducted to examine coffee consumption behaviors, dietary habits, and nutrient intakes by coffee intake amount among university students. Methods: Questionnaires were distributed to 300 university students randomly selected in Gongju. Dietary survey was administered during two weekdays by the food record method. Results: Subjects were divided into three groups: NCG (non-coffee group), LCG (low coffee group, 1~2 cups/d), and HCG (high coffee group, 3 cups/d) by coffee intake amount and subjects' distribution. Coffee intake frequency was significantly greater in the HCG compared to the LCG (p < 0.001). The HCG was more likely to intake dripped coffee with or without milk and/or sugar than the LCG (p < 0.05). More than 80% of coffee drinkers chose their favorite coffee or accompanying snacks regardless of energy content. More than 75% of coffee takers did not eat accompanying snacks instead of meals, and the HCG ate them more frequently than LCG (p < 0.05). Breakfast skipping rate was high while vegetable and fruit intakes were very low in most subjects. Subjects who drank carbonated drinks, sweet beverages, or alcohol were significantly greater in number in the LCG and HCG than in the NCG (p < 0.01). Energy intakes from coffee were $0.88{\pm}5.62kcal/d$ and $7.07{\pm}16.93kcal/d$ for the LCG and HCG. For total subjects, daily mean dietary energy intake was low at less than 72% of estimated energy requirement. Levels of vitamin C and calcium were lower than the estimated average requirements while that of vitamin D was low (24~34% of adequate intake). There was no difference in nutrient intakes by coffee intake amount, except protein, vitamin A, and niacin. Conclusion: Coffee intake amount did not affect dietary nutrient intakes. Dietary habits were poor,and most nutrient intakes were lower than recommend levels. High intakes of coffee seemed to be related with high consumption of sweet beverages and alcohol. Therefore, it is necessary to improve nutritional intakes and encourage proper water intake habits, including coffee intake, for improved nutritional status of subjects.

• Journal of Bio-Environment Control
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• v.18 no.4
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• pp.436-441
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• 2009

The experiment was conducted to investigate the effect of nitrogen and potassium sources on growth and yield of strawberry 'Seolhyang' and 'Maehyang' in fertigation culture. The plant fresh and dry weight were higher in urea + potassium sulfate and ammonium sulfate + potassium sulfate as nitrogen and potassium sources than others in both varieties. But there were no significant difference among nitrogen and potassium sources in other growth characteristics, such as plant height, no. of leaf, crown diameter etc. Also, the marketable yield of fruit were higher in urea + potassium sulfate and ammonium sulfate + potassium sulfate in 'Seolhyang' than other treatments. 'Maehyang' was better in both treatments but there was no significant difference. The fruit qualities, such as total soluble solid, hardness, acidity, vitamin C content were not significant difference among the treatments. The acidity of soil was tended to decrease but EC was tended to increase highly in ammonium sulfate treatments as a nitrogen source. As above results, the urea and potassium sulfate were recommended as nitrogen and potassium sources for the fertigation culture of strawberry 'Seolhyang' and 'Maehyang'.

• Korean Journal of Food Science and Technology
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• v.41 no.6
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• pp.712-716
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• 2009

In this study, the antioxidant and neuronal cell protective effects of methanol extract from Schizandra chinensis were evaluated. The proximate composition and total phenolics content of the extract were as follows: 64.88% nitrogen free extract, 10.56% crude fiber, 10.22% moisture, 8.33% crude protein, 5.05% ash, 0.96% crude fat, and 83.04 mg/g of total phenolics. In assays the methanol extract of Schizandra chinensis presented ferric reducing/antioxidant power (FRAP) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity in a dose-dependent manner. In a cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT), the methanol extract showed protective effect against $H_2O_2$-induced neurotoxicity, and lactate dehydrogenase (LDH) release into medium was also inhibited by various concentrations of extracts (68-80%). Cell viability after treatment of the methanol extract was higher than that shown for vitamin C ($100\;{\mu}M$) using a neutral red uptake (NRU) assay. Therefore, these data suggest that the methanol extract of Schizandra chinensis may be useful for neurodegenerative diseases including Alzheimer's disease.

• Korean Journal of Environmental Agriculture
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• v.17 no.3
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• pp.189-194
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• 1998

In order to evaluate the residual effects of bituminous coal fly ash, gypsum, oyster shell as soil amendments, Chinese cabbage was cultivated on acidic sandy loam soils with low boron content. The soils were amended in the upper 15cm with amendments in previous year and the growth and qualities of plants were analyzed. Amendments treated one year before were, in metric tons per hectare, i) none(Check) ; ii) 80 fly ash(FA) ; iii) 4 shell(SH) ; iv) 56 fly ash + 24 gypsum (FG) ; v) 40 fly ash + 24 gypsum + 0.8 shell(FGS). Yield response in fresh weight of Chinese cabbage was in order of 834% for FGS > 780% for FG > 755% FA > 193% for SH plants. Reducing sugar, vitamin-C, and total nitrogen contents of leaves depending on treatments showed the same tendencies as that in yields, whereas crude fibre opposite to them. In particular, FA, FG, and FGS plants showed normal growth without both boron deficiency symptoms which appeared in Check and SH plants and possibilities of accumulation of heavy metals. In any soils treated with the above amendments, however, magnesium was insufficient.

• Korean Journal of Plant Resources
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• v.29 no.2
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• pp.163-170
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• 2016

Antioxidant activity and inhibitory effect on oxidative DNA damage of ethyl acetate fractions extracted from Cone of Red Pine (Pinus densiflora) were investigated to find utilization of Cone, by-product of Red Pine, thrown out after berry shatter, as a new natural plant resource. Cone from P. densiflora was extracted with methanol (MeOH) and separated to petroleum ether, ethyl acetate and water fraction. Among them, ethyl acetate fraction was used. The antioxidant activity was conducted by the 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, 2, 2'-Azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) radical scavenging assay, Fe²⁺ chelating assay and reducing power assay. The inhibitory effect on oxidative DNA damage was determined by DNA cleavage assay using φX-174 RF I plasmid. The results of DPPH and ABTS radical scavenging activity at 200 ㎍/㎖ of extracts were 86.50% and 95.80% respectively, which were similar figures compared with L-ascorbic acid as control. Fe²⁺ chelating activity was 77.96% and reducing power was 0.77 at 200 ㎍/㎖. Total phenolic component was 27.29±0.3 ㎎/g and Vitamin C content was 1.84±0.1 ㎎/g. Also ethyl acetate fraction from Cone has inhibitory effect, using φX-174 RF I plasmid on DNA cleavage assay. In conclusion, Cone, by-product of P. densiflora, showed high antioxidant activity and inhibitory effect on oxidative DNA damage. Therefore this study suggests Cone, useless by-product, can be developed as a new natural plant resource with lots of utilization such as an effective antioxidant, natural medicine, food, cosmetics and so on.

• Journal of Bio-Environment Control
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• v.4 no.1
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• pp.68-73
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• 1995

This study was carried out to investigate the effect of the concentration of nutrient solution on growth of tomato(Lrcopersicon esculentum Mill. cv. seokwang) in substrate culture. The substrates used in the experiment were perlite, vermiculite, and peatmoss. Tomato plants were treated with different concentrations of nutrient solution, viz. 0.5, 1.0, 2.0, 3.0, and 5.0mS/cm at seedling stage and transferred to different treatments, 1.0, 2.0, and 3.0mS/cm after transplanting in each substrate. Total fruit number, total yield, marketable fruit number, and marketable yield were much higher at 2.0-3.0mS/cm than at 1.0mS/cm in all three substrate culture. The percentage of malformed fruit was in order of peatmoss>perlite> vermiculite and Vitamin C content was vermiculite> perlite> peatmoss. In all three substrate culture, high marketable yield was shown when tomato plants grew with concentration of 2.0-5.0mS/cm at seedling stage and 2.0-3.0mS/cm after transplanting. The concentrations of nutrient solution after transplanting as well as at seedling stage had a great influence on total yield, marketable yield, and soluble solids. However total and marketable fruit number were considerably affected by the concentrations of nutrient solution after transplanting.

• Korean Journal of Food Science and Technology
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• v.31 no.5
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• pp.1196-1202
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• 1999

This work was to determine the microbial and physico-chemical changes of HTST-pasteurized soymilk using microwave energy. Soymilk was HTST pasteurized$(at\;90^{\circ}C\;for\;20\;sec)$ by three methods: by heating in a stainless steel tube immersed in a hot water bath(MP0), by heating in a microwave cavity to a defiled temperature and then holding in a hot water bath(MP1), and by both heating and holding in a microwave cavity(MP2). The microbial quality based on the total plate count was in the order of MP0, MP2 and MP1. The three samples pasteurized by different methods showed the similar microbial quality with respect to the coliform count, psychrotrophic bacterial count and phosphatase activity. The destruction of trypsin inhibitor was in the order of MP0, MP1 and MP2. There were no significant differences in pH, titratable acidity, viscosity and vitamin $B_2$ content before and after pasteurization and among the different pasteurization methods. The similar or higher quality retention of the MP1 or MP2 supports the possibility of using microwave energy for the HTST pasteurization of soymilk and other fluid food products.

• Korean Journal of Medicinal Crop Science
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• v.9 no.4
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• pp.275-279
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• 2001

The contents of chemical components such as total nitrogen, total amino acid, chlorophyll, vitamin C and free sugar were somewhat higher in $CO_2$ and $N_2$ gas treatment than those of other treatment. However, the contents of tannin and caffeine did not show any different in the 5 treatments. ${\gamma}-aminobutyric$ acid(GABA) and alanine accumulated in tea leaves under anaerovic condition. The content of GABA acid with ${CO_2\;and\;N_2}$ gas treatment was higher 8-6 times with values of ${264{\sim}215mg/100g}$ than in control (35mg/100g). The scores of sensory test was not different between anaerobic treatment and control. Consequently, tea mading within ${N_2\;and\;CO_2}$ gas treatment after plucking was considered to be the best green tea in terms of functional nature as well as taste nature.

• Korean Journal of Environmental Agriculture
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• v.17 no.2
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• pp.164-169
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• 1998

In order to evaluate the utility of the bituminous coal fly ash, gypsum, oyster shell as soil amendments, acid sandy loam soil with low boron content were amended in the upper 15cm with amendments, and then chinese cabbage was cultivated in fall. Amendments treated were, in metric tons per hectare, i ) none(Check) ; ii) 80 fly ash(FA) ; iii) 4shell(SH) ; iv) 56 fly ash+24gypsum (FG) ; v) 40 fiy ash + 24 gypsum +0.8 shell(FGS). On the whole, amendments imoroved soil chemical properties and contents of N, P, K, Ca, and B in leaves. Among treatmens, FA prominently neutralized soil pH and increased available $P_2O_5$ ,B but decreased Fe contents in soils. FGS also affected the increment of exchangeable Ca, Mg, and available B. Yield response in fresh weight of chinese cabbage was in order of 85% for FGS>77% for FG>66% FA>5% for SH plants. Reducing sugar and vitamin-C contents of leaves depending on treatments showed the same tendencies as that in yields, whereas crude fiber opposite to theme. In particular, FA, FG, and FGS plants showed normal growth without boron deficiency symotoms which appeared in Check and SH plants.Taken together, FGS was an effective combination enable to maximize the utility of fly ash, gypsum, and shell as soil amemdments, especially in cabbage yield and quality.