• The Korean Journal of Nuclear Medicine Technology
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• v.24 no.1
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• pp.33-38
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• 2020

Purpose Vitamin D is essential for maintaining bone health, controling cell proliferation or differentiation, strengthening immune function by controlling calcium metabolism in the body. Vitamin D deficiency can lead to increase the risk of rickets, osteoporosis, cardiovascular disease, diabetes and cancer. Especially, South Korea is one of the highest population proportion of vitamin D deficiency. Accurate determination of levels of 25-OH-VitD or 25-OH-VitD3 in blood serum is required for the diagnosis and treatment of vitamin D deficiency. In this study, radioimmunoassay of 25-OH-VitD and 25-OH-VitD3 was performed and compared to evaluate the effectiveness of Vitamin D radioimmunoassay. Materials and Methods Serum 25-OH-VitD and 25-OH-VitD3 levels were measured using radioimmunoassay. The interrelationship, reproducibility and population distribution rate were evaluated. In addition, the internal quality control was performed at Asan Medical Center from April 2017 to June 2019 and the result of external quality control (Interagency proficiency evaluation) of first and second half of 2018 hosted by the Korean Society of Nuclear Medicine Technology (KSNMT). Both tests were measured by same manufacturer's reagent. Results 25-OH-VitD showed a strong positive correlation on 97 samples, as 25-OH-VitD3 x 0.9 + 0.3 (R>0.9). In repeated measurement, the average Diff(%) value of the reproducibility evaluation of 25-OH-VitD and 25-OH-VitD3 were 7.7% and 7.4%, respectively. Population distribution results showed no statistically significant differences(p>0.05). The resultant value of internal quality control, which measured from April, 2017 to June 2019 in Blood test room of Nuclear Medicine at Asan Medical Center, showed average (CV%) 6.2% and 6.8%, respectively. As a result of the external quality control (interagency proficiency evaluation) Z value obtained under 2.0, as shown below; Conclusion The interrelationship, reproducibility, population distribution rate, internal quality control and external quality control between 25-OH-VitD and 25-OH-VitD3 radioimmunoassay shows superior outcome. Radioimmunoassay, which can be alone measured in the blood as 25-OH-VitD or 25-OH-VitD3, is considered suitable screening tests for the diagnosis of vitamin D deficiency.

• Applied Microscopy
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• v.32 no.3
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• pp.223-230
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• 2002

Vitamin D is one of important factors involved in the regulation of bone metabolism. In osteoporosis, the therapeutic effect of vitamin D on the healing process has still been controversial. To confirm the effects of $1{\alpha},\;25\;Dihydroxyvitamin\;D_3$ on the osteoporosis, the change of bone mineral density and the histologic changes of osteoporotic femur were examined comparatively in normal control group (positive control), CFA control group (negative control), CFA+$1{\alpha}$, 25 dihydroxyvitamin $D_{3}\;0.01{\mu}g/kg$ group (Vit $D_{3}L$) and CFA+$1{\alpha}$, 25 dihydroxyvitamin $D_{3}\;0.1{\mu}g/kg$ group (Vit $D_{3}H$) after osteoporosis was induced by single injection of complete Freund's adjuvant (CFA) in rats. The value of bone mineral density and bone mineral content of femur was increased in both Vit $D_{3}L$ and Vit $D_{3}H$ than CFA control, and the increase rate of that was higher in Vit $D_{3}H$ than Vit $D_{3}L$. In CFA control, the size of the bone marrow cavities significantly increased and the width from the bone marrow cavity and cortex significantly decreased than normal control. In Vit $D_{3}H$ and Vit $D_{3}L$, the increase rate of the size of bone marrow cavity and the decrease rate of the width from the bone marrow cavity and cortex was depressed than CFA control. These results suggest that $1{\alpha},\;25\;Dihydroxyvitamin\;D_3$ has therapeutic effect on adjuvant-induced osteoporosis in rats.

• Korean journal of food and cookery science
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• v.13 no.2
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• pp.173-178
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• 1997

The contents of ergocalcife.of (vit D$_2$) and cholecalciferol (vit D$_3$) in mushrooms (Lentinus edodes, Agaricus bisporus, Pleurotus ostreatus, Flammulina velutipes, Auricularia auricular, Gyropora esculenta, Romaria botftis, Coriorus versicolar, Ganoderma lucidum) were determined by high-performance liquid chromatography (HPLC), using external standard method. The methods included saponification, extraction, drying, filtering and quantification with analytical HPLC (waters Inc.). The contents of vit D$_2$ and D$_3$ found in different mushroom species. A. auricular, and L. edodes contained high amounts of vit D, 167.8, 72.6 $\mu\textrm{g}$/100 g of edible portion, respectively. On the other hand A. bisporus showed the lowest contents of vit D among analyzed mushrooms.

• Advances in Traditional Medicine
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• v.9 no.3
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• pp.259-267
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• 2009

Curcumin (CMN) is known to have beneficial role in anorexia, coryza, cough, diabetic wounds, and hepatic disorders apart from its inherent antioxidant effects. Therefore, the present study was aimed to evaluate antioxidant effect of CMN in prevention of nephrolithiasis in rats-induced by ethylene glycol (EG) and Vitamin D3 (Vit. D3). Male Wistar rats (175 - 200 g) were randomized in groups like control, EG + Vit. D3 induced nephrolithiatiatic rats, CMN treated rats, CMN + EG + Vit. D3 treated rats, Vit. E + EG + Vit. D3 treated rats. Urine was collected weekly throughout the experimental protocol and estimated for calcium oxalate (CaO) count. After completion of experimental protocol serum was estimated for blood urea nitrogen and creatinine. Both the kidneys were excised and used to evaluate levels of biomarkers of oxidative stress and calcium oxalate crystal deposition by histopathological studies. Administration of EG and Vit. D3 to rats resulted in increased oxidative stress, hyperoxaluria and renal deposition of CaO crystals. Supplementation with CMN improves kidney function, reduces elevated oxidative stress, urinary oxalate level and renal deposition of CaO which shows its protective action in nephrolithiasis. The increased deposition of stone in the kidney and stone forming constituents of nephrolithiatic rats were effectively lowered by treatment of CMN.

• Korean Circulation Journal
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• v.53 no.2
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• pp.92-102
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• 2023

Background and Objectives: YKL-40 is considered to be associated with cardiovascular disease (CVD). In this study, the effect of serum 25(OH) vitamin D [25(OH)VitD] differences between groups on YKL-40 was evaluated on a hypercholesterolemia rat model. Methods: Thirty-two male rats (wistar albino) were equally divided into 4 groups. The first group was the control group; the second group was high-cholesterol (H-CH) adequate vitamin D (VitD) group (H-Ade^VD). The third group was the H-CH deficient VitD group (H-Def^VD), and the last group was designed with the H-CH supplement VitD (H-Sup^VD). The feeding process consisted of 2 stages. At the first stage (5 months), the H-Def^VD group was fed on VitD deficient chow, while the other groups (control, H-Ade^VD, H-Sup^VD) were fed on standard chow. At the second stage (3 months), the H-Ade^VD and the H-Sup^VD groups were fed on the H-CH chow, whereas the H-Def^VD group was fed on the H-CH-VitD deficient chow. Moreover, the H-Sup^VD group was given 100 IU/kg/day VitD along with the H-CH chow. Results: Compared with the control group, interleukin-6 (IL-6), soluble intercellular adhesion molecule-1 (sICAM-1), soluble vascular cell adhesion molecule-1 (sVCAM-1), and YKL-40 values in the H-Def^VD groups increased significantly (p<0.001, p<0.001, p=0.009, p=0.005; sequentially). Conclusion: It can be concluded that VitD can suppress the YKL-40, thus, it will prevent CVD development in rat. Therefore, further clinical studies related with human will reveal the effect of VitD and YKL-40 on CVD development.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.6
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• pp.2743-2748
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• 2013

The vitamin gets through food and the sun. But there is case that density alters by certain factor in vitamin. So this research analyzed effect of the liver function factor and change of $25OH-VIT.D_3$ density. Research target is a person who undergo health medical examination in the certain university hospital medical examination center from August, 2012 to December, 2012. Comparison classified to experiment group and control group. As a result, variable that influence in $25OH-VIT.D_3$ density showed by age and B type inflammation of the liver titer. Specially, B type inflammation of the liver titer displayed reverse corelation ($R^2$=0.40). Therefore, inflammation of the liver carrier must manage liver fuction test and density of $25OH-VIT.D_3$.

• Journal of the Korean Society of Clothing and Textiles
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• v.22 no.5
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• pp.646-653
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• 1998

Because there is a great concern' today about the damaging effect of chronic exposure to sunlight the use of sunscreen providing the photoprotection effect against ultraviolet (UV) was widely increased. As a result of common use of level of photosynthetic Vit. D3 in human skin decreased these days. In our experiment the animals covered with fabrics with 50% (fabric B) and 100% (fabric A) protection rate against ultraviolet B (UVB) were used to measure serum 25(OH)D3, ALP, total clacium and phosphorus. Vitamin D deficiency diet group had no effect on concentration of serum phosphorus. But the concentrations of serum 25(OH)D9 and total calcium were more decreased in vitamin D deficiency diet rats than in normal diet rats. Alkaline phosphatase activity in sunlight irradiated groups covered with 50% (fabric B) and 100% (fabric A) WB protection fabrics was more significantly decreased than vitamin D deficiency diet group. In conclusion, sunlight irradiateted groups were compared to effective to protect born disease due to the Vit. D deficeincy group.

• Korean Journal of Animal Reproduction
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• v.27 no.2
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• pp.143-152
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• 2003

The objective of this study were to investigate the effects of Se and Vit. E administration on blood chemical values and Vit. E concentrations of serum in calves born from Hanwoo dams injected Se and Vit. E at late pregnancying periods. The effects of Se and Vit. E administration of blood chemical values in calves born from Hanwoo dams injected Se and Vit. E at the last month of pregnancying periods were examined. In albumin concentration of blood, the Vit. E 2000IU group were slightly higher than other groups and the cholesterol concentrations were also higher in Vit. E 15001u group than other groups. But, another components examined of blood chemical values were not significantly different in all experimental groups. The blood chemical values in calves born from Hanwoo dams administrated Se and Vit. E at two months before parturition were examined. However, there were not significantly different in all experimental groups in concentrations of albumin, cholesterol, BUN, creatinine, glucose, inorganic phosphorous, calcium, total protein and triglycerides. The concentrations of serum Vit. E in calves born from Hanwoo dams administrated Se and Vit. E at 30 and 15 days before parturition were examined. The concentrations of Vit. E in serum were higher in treatment groups than in control group(P>0.05). The concentrations of serum Vit. E in calves born from Hanwoo dams administrated Se and Vit. E at 60 days before parturition were examined, the concentrations of Vit. E in serum were slightly higher in groups administrated than in control group(P>0.05), but there were not significantly difference in all experimental groups.

• Journal of the Korean Society of Clothing and Textiles
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• v.23 no.7
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• pp.980-986
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• 1999

This study was done to ivestigate the fabrics thatminimized harmfulness of UVB(ultraviolet B) and that might product Vit. {{{{ {D }`_{3 } ^{ } }} by UVB. Twelve female subjects wearing in three different types i.e fabric A(UVB 100% protection) fabric B(UVB 50% protection) and bikinii were exposed to outdoor environment (Air Temp : 25℃, 42% R,H Air velocity : 0.13m/s UV does :6KJ/m2) Blood samples were taken 24 hours before the after the experiment in order to examine concentration of vit.{{{{ {D }`_{3 } ^{ } }} in the blood. During the experiment axillary temperature skin temperature of 7 areas(forehead Chest Upper arm, Hand Thigh Lower leg, Foot) were measured. The more irradiated areas by UVB were the more the concentration of serum 25(OH){{{{ {D }`_{3 } ^{ } }} were significantly. Mean skin temperature was significantly low levekl in wearing the fabric of UVB 50% protection (p<0.001) Axillary temperature was significantly high level in wearing the fabric of UVB 50% protection (p<0.001). Therefore the fabric of UVB 50% protection intercepts the radiation and has advantage to give off body heat over other fabrics

• Journal of Nutrition and Health
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• v.29 no.9
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• pp.943-949
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• 1996

Bone mineral density depends largely on the status of dietary minerals such as Ca, P, Mg, and F and proteins, physical activities, parathyroid hormone(PTH), calcitonin(CT), and vitamin D. The decrease of bone density often results in bone fractures and osteoporosis which is prevalent among postmenopausal women. This study was intended to examine the role of parathyroid hormone, calcitonin and cholecaliferol in bone density of mice that were fed different dual photon energy beams. We have measured three major parts of the bone : whole body, head and femur. The results are summarized as follows : 1) Bone mineral density (BMD) was more increased by feeding high Ca diet compared to that of the low Ca diet. 2) Both PTH and Vit D3 enhanced BMD in all of the different Ca levels. 3) When the dietary Ca was deequate CT showed a synergistic effect with PTH in boosting bone density, while CT+Vit D3 showed a negative effect. 4) CT tended to inhibit the effect of increasing bone density by PTH and Vit D3 in medium and low Ca groups. 5) The effect of increasing bone density by PTH in the head of mouse increased when dietary Ca was lower : The increment of bone density by PTH in high, medium, and low Ca was 3%, 8%, 19%, respectively. 6) Femur bone density was affected significantly by dietary Ca levels than hormones. The above observations indicate that bone mineral density can be improved by high dietary Ca and hormone injections including PTH, CT and cholecalciferol, and thus proper dietary and hormonal treatment may be used in preventing bone fractures and osteoporosis.