• Clinical and Experimental Reproductive Medicine
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• v.24 no.3
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• pp.335-340
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• 1997

Fluorescence in situ hybridization (FISH) techniques allow the enumeration of chromosome abnormalities and from a great potential for many clinical applications. In order to produce quantitative and reproducible results, expensive tools such as a cooled CCD camera and a computer software are required. We have developed a Chromosome Image Processing System (Chips) using FISH that allows the detection and mapping of the genetic aberrations. The aim of our study, therefore, is to evaluate the capabilities of our original system using a black-and-white video camera. As a model system, three repetitive DNA probes (D18Z1, DXZ1, and DYZ3) were hybridized to variety different clinical samples such as human metaphase spreads and interphase nuclei obtained from uncultured peripheral blood lymphocytes, uncultured amniocytes, and germ cells. The visualization of the FISH signals was performed using our system for image acquisition and pseudocoloring. FISH images were obtained by combining images from each of probes and DAPI counterstain captured separately. Using our original system, the aberrations of single or multiple chromosomes in a single hybridization experiment using chromosomes and interphase nuclei from a variety of cell types, including lymphocytes, amniocytes, sperm, and biopsied blastomeres, were enabled to evaluate. There were no differences in the image quality in accordance with FISH method, fluorochrome types, or different clinical samples. Always bright signals were detected using our system. Our system also yielded constant results. Our Chips would permit a level of performance of FISH analysis on metaphase chromosomes and interphase nuclei with unparalleled capabilities. Thus, it would be useful for clinical purposes.

• Journal of Gifted/Talented Education
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• v.15 no.1
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• pp.67-84
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• 2005

In this study we investigated the adequacy of tools for distinction gifted students through the comparison these mutual relation on the basis of data, like paper test, the depths interview score, and the rest data((TTCT: Torrance Tests of Creative Thinking, IQ test, FASP: Find A Shape Puzzle, V.T: Visualization Tests and Exp: experimental ability test), and analysis data of EEG test for examining the adequacy of tools for identification gifted students. So, we developed Brain Wave gifted Index(G-Index) for finding another distinction ability as using brain waves data. The standard of index development use gifted brain characteristic in closed-eyes rest state which is judged like that characteristic of distinction between gifted and normal students is the most clear and consistence. That is, the degree of unified pattern between each object and gifted PCA pattern was defined by Pearson method which added spatial mutual index to weight concept. This refer to mean number of spatial PCA pattern. Searching for the possibility of distinction gifted gave distinction effect in 76%. The result of regression analysis on the basis of mutual relation between the rest data is . The probability formula for distinct gifted group is as follow. $$P=\frac 1{1+e^{-[-0.018(TTCT)+0.057(IQ)+1.916(FASP)+0.682(V.T)+0.088(Exp.)+0.034(G-Index)-57.510]}}$$ The result of this calculation showed that probability for distinct in gifted group was very good(95.0%). On the basis of upper result, tools for identification gifted students should be estimated as using many-sided estimation data whatever possible. And following study about development, and operation of tools for distinction suitable to gifted student in science should be progressed.

• The KIPS Transactions:PartA
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• v.11A no.5
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• pp.391-400
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• 2004

Within multimodal medical image registration techniques, which correlate different images and Provide integrated information, surface registration methods generally minimize the surface distance between two modalities. However, the features of two modalities acquired from one subject are similar. So, it can improve the accuracy of registration result to match two images based on optimization of both surface distance and shape feature. This research proposes a registration method which optimizes surface distance and surface curvature of two brain modalities. The registration process has two steps. First, surface information is extracted from the reference images and the test images. Next, the optimization process is performed. In the former step, the surface boundaries of regions of interest are extracted from the two modalities. And for the boundary of reference volume image, distance map and curvature map are generated. In the optimization step, a transformation minimizing both surface distance and surface curvature difference is determined by a cost function referring to the distance map and curvature map. The applying of the result transformation makes test volume be registered to reference volume. The suggested cost function makes possible a more robust and accurate registration result than that of the cost function using the surface distance only. Also, this research provides an efficient means for image analysis through volume visualization of the registration result.

• The KIPS Transactions:PartA
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• v.8A no.4
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• pp.509-516
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• 2001

As results of many genome projects, genomic sequences of many organisms are revealed. Various methods such as global alignment, local alignment are used to analyze the sequences of the organisms, and k -mer analysis is one of the methods for analyzing the genomic sequences. The k -mer analysis explores the frequencies of all k-mers or the symmetry of them where the k -mer is the sequenced base with the length of k. However, existing on-memory algorithms are not applicable to the k -mer analysis because a whole genomic sequence is usually a large text. Therefore, efficient data structures and algorithms are needed. String B-tree is a good data structure that supports external memory and fits into pattern matching. In this paper, we improve the string B-tree in order to efficiently apply the data structure to k -mer analysis, and the results of k -mer analysis for C. elegans and other 30 genomic sequences are shown. We present a visualization system which enables users to investigate the distribution and symmetry of the frequencies of all k -mers using CGR (Chaotic Game Representation). We also describe the method to find the signature which is the part of the sequence that is similar to the whole genomic sequence.

• Journal of Korean Neurosurgical Society
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• v.29 no.4
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• pp.461-470
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• 2000

Objective : Many investigators have demonstrated the protective effects of hypothermia following traumatic brain injury(TBI) in both animals and humans. It has long been recognized that mild to moderate hypothermia improves neurologic outcomes as well as reduces histologic and biochemical sequelae after TBI. In this study, two immunohistochemical staining using terminal deoxynucleotidyl-transferase-mediated biotin dUTP nick end labeling(TUNEL), staining of apoptosis, and ${\beta}$-amyloid precursor protein(${\beta}$-APP), a marker of axonal injury, were done and the authors evaluated the protective effects of hypothermia on axonal and neuronal injury after TBI in rats. Material and Method : The animals were prepared for the delivery of impact-acceleration brain injury as described by Marmarou and colleagues. TBI is achieved by allowing of a weight drop of 450gm, 1 m height to fall onto a metallic disc fixed on the intact skull of the rats. Fourty Sprague-Dawley rats weighing 400 to 450g were subjected to experimental TBI induced by an impact-acceleration device. Twenty rats were subjected to hypothermia after injury, with their rectal temperatures maintained at $32^{\circ}C$ for 1 hour. After this 1-hour period of hypothermia, rewarming to normothermic levels was accomplished over 30-minute period. Following 12 hours, 24 hours, 1 week and 2 weeks later the animals were killed and semiserial sagittal sections of the brain were reacted for visualization of the apoptosis and ${\beta}$-APP. Results : The density of ${\beta}$-APP marked damaged axons within the corticospinal tract at the pontomedullary junction and apoptotic cells at the contused cerebral cortex were calculated for each animal. In comparison with the untreated controls, a significant reduction in ${\beta}$-APP marked damaged axonal density and apoptotic cells were found in all hypothermic animals(p<0.05). Conclusion : This study shows that the posttraumatic hypothermia result in substantial protection in TBI, at least in terms of the injured axons and neurons.

• Journal of the Korean Association of Geographic Information Studies
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• v.5 no.1
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• pp.58-68
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• 2002

Recently as recognition to prevent nature disasters is reaching the climax, the most important job of government official is to provide information related to the prevention of nature disasters through the Web and to bring notice to prevent disaster under people. Especially, if the case of daily forest fire hazard index is provided within visualization on Web, people may have more chances to understand about forest fire and less damages by large scale of forest fire. Forest fire hazard index presentation system developed in this paper presents daily forest fire hazard index on map visually also provides the information related to it in text format. In order to develop this system, CBDP(Component Based Development Process) is proposed in this paper. This development process tries to emphasize the view of reusability so that it has lifecycle which starts from requirement and domain analysis and finishes to component generation. Moreover, The concept of this development process tries to reflect component based method, which becomes hot issue in software field nowadays. In the future, the component developed in this paper may be possibly reused in other Web GIS application, which has similar function to it so that it may take less cost and time to develop other similar system.

• Korean Journal of Remote Sensing
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• v.26 no.5
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• pp.561-570
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• 2010

The purpose of this study is to develop an Android smartphone app providing analysis capabilities of remote sensing images, by using mobile browsing open sources of gvSIG, open source remote sensing software of OTB and open source DBMS of PostgreSQL. In this app, five kinds of remote sensing algorithms for filtering, segmentation, or classification are implemented, and the processed results are also stored and managed in image database to retrieve. Smartphone users can easily use their functions through graphical user interfaces of app which are internally linked to application server for image analysis processing and external DBMS. As well, a practical tiling method for smartphone environments is implemented to reduce delay time between user's requests and its processing server responses. Till now, most apps for remotely sensed image data sets are mainly concerned to image visualization, distinguished from this approach providing analysis capabilities. As the smartphone apps with remote sensing analysis functions for general users and experts are widely utilizing, remote sensing images are regarded as information resources being capable of producing actual mobile contents, not potential resources. It is expected that this study could trigger off the technological progresses and other unique attempts to develop the variety of smartphone apps for remote sensing images.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.3
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• pp.520-525
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• 2019

Mines are an important infrastructure for securing resources, but safety problems can arise in the course of operation. Recently, the mining process is very complicated due to the large scale and mechanization. Therefore, it is necessary to construct accurate geospatial information on mine for systematic and safe mine operation. The geospatial information construction using the existing total station has a disadvantage that a lot of work time is required because the target must be collimated and measured. In this study, the data of the mines were acquired with the total station and the 3D laser scanner, and the mine spatial information was constructed by using the shape based registration method. By using the static scanner data of some area applying the reference point surveying result of the total station, it was possible to construct the accurate result on the wide area acquired by the mobile scanner effectively. Also, the accuracy of the constructed geospatial information was evaluated and the deviation of mean 0.083m was shown. Point cloud products constructed through the research can contribute to the efficiency improvement of mine management by enabling quantitative analysis such as visualization of mine shape, distance, area and slope, and automation of drawing creation for cross section shape.

• Journal of the Korean Society of Radiology
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• v.13 no.2
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• pp.209-216
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• 2019

The purpose of this study was to suggest the optimum ambient illumination level for proper visualization in image inspection and reading on CRT and LCD monitors used for ultrasound and reading. The evaluators were divided into 4 groups: 20 (Ultra-sonographer: 20 groups (4 groups: ultra-sonographer, 1-5 years, 5 ultra-sonographers, 6 to 10 years, 5 ultra-sonographers, 11 to 15 years, The subjects were 32 questions. The evaluation method was image evaluation of ultrasonic soft copy images for 30 seconds per 10, 25, 100Lux ambient illumination. The evaluation results were evaluated as 6 points (Normal = Definitely no lesion), 2 points = possibly not a lesion, 3 points = probably not a lesion, 4 points = possibly a lesion, 5 points = probably a lesion, 6 points = Definitely a lesion). In this study, the results of ROC analysis according to ambient light illumination reading softcopy images used for lesion detection of all ultrasound images showed the highest sensitivity, specificity, and AUC results at 10Lux. It was found that optimal use of 10Lux for ambient light illumination would provide optimal detection of lesions in ultrasound soft copy images. Based on the future research data, it will be presented as basic data for designing ambient light brightness of ultrasound imaging laboratory and reading room.

• Korean Journal of Clinical Laboratory Science
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• v.51 no.2
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• pp.177-184
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• 2019

Mycobacteria grow slowly. Therefore, a solid medium should be used for eight weeks and a liquid medium for six weeks. The purpose of this study was to find the growth factors that can grow Mycobacterium rapidly and to help develop a solid medium for rapid identification. Three types of Mycobacterium growth factors were evaluated with 10 Mycobacteria by adding activated charcoal, defibrinated sheep blood, and L-ascorbic acid to $Difco^{TM}$ Mycobacteria 7H11 agar (Becton, Dickinson and Company, Sparks, MD, USA). The time to detection and the distinguishability of a colony were compared with that of the current method. In the rapidly growing Mycobacterium, the difference in detection time between the new media and conventional media confirmed that the new media was faster. M. kansasii and M. intracelluare grew faster in 7H11 C than in 7H11 medium. MTB grew faster than the other media in 7H11 C. This study confirmed that the two growth factors affect fast-growing Mycobacteria and slow-growing Mycobacteria. 7H11 C showed better distinguishability than the conventional media in all 10 Mycobacterium due to the color contrast. In particular, when the MTB was grown, the size of the colonies was larger than with other media, so visualization was easy.