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http://dx.doi.org/10.15324/kjcls.2019.51.2.177

Study on the Growth Factors for Rapidly Cultivating Mycobacterium spp.  

Ha, Sung-Il (Department of Laboratory Medicine, The Catholic University of Korea, Seoul Saint Mary's Hospital)
Park, Kang-Gyun (Department of Laboratory Medicine, The Catholic University of Korea, Seoul Saint Mary's Hospital)
Suk, Hyun-Soo (Department of Laboratory Medicine, The Catholic University of Korea, Seoul Saint Mary's Hospital)
Shin, Jeong-Seob (Department of Laboratory Medicine, The Catholic University of Korea, Seoul Saint Mary's Hospital)
Shin, Dong-Pil (Department of Laboratory Medicine, The Catholic University of Korea, Seoul Saint Mary's Hospital)
Kwon, Min-O (Department of Laboratory Medicine, The Catholic University of Korea, Seoul Saint Mary's Hospital)
Park, Yeon-Joon (Department of Laboratory Medicine, The Catholic University of Korea, College of Medicine)
Publication Information
Korean Journal of Clinical Laboratory Science / v.51, no.2, 2019 , pp. 177-184 More about this Journal
Abstract
Mycobacteria grow slowly. Therefore, a solid medium should be used for eight weeks and a liquid medium for six weeks. The purpose of this study was to find the growth factors that can grow Mycobacterium rapidly and to help develop a solid medium for rapid identification. Three types of Mycobacterium growth factors were evaluated with 10 Mycobacteria by adding activated charcoal, defibrinated sheep blood, and L-ascorbic acid to $Difco^{TM}$ Mycobacteria 7H11 agar (Becton, Dickinson and Company, Sparks, MD, USA). The time to detection and the distinguishability of a colony were compared with that of the current method. In the rapidly growing Mycobacterium, the difference in detection time between the new media and conventional media confirmed that the new media was faster. M. kansasii and M. intracelluare grew faster in 7H11 C than in 7H11 medium. MTB grew faster than the other media in 7H11 C. This study confirmed that the two growth factors affect fast-growing Mycobacteria and slow-growing Mycobacteria. 7H11 C showed better distinguishability than the conventional media in all 10 Mycobacterium due to the color contrast. In particular, when the MTB was grown, the size of the colonies was larger than with other media, so visualization was easy.
Keywords
Activated charcoal; Defibrinated sheep blood; L-ascorbic acid;
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1 Korea Centers for Disease Control and Prevention. Guidelines for national tuberculosis control, 2018. Cheong-Ju: Korea Centers for Disease Control and Prevention; 2018. p470.
2 Aitken ML, Limaye A, Pottinger P, Whimbey E, Goss CH, Tonelli MR, et al. Respiratory outbreak of Mycobacterium abscessus subspecies massiliense in a lung transplant and cystic fibrosis center. Am J Respir Crit Care Med. 2012;185:231-2. https://doi.org/10.1164/ajrccm.185.2.231.   DOI
3 American Thoracic Society. Diagnosis and treatment of disease caused by nontuberculous mycobacteria. Am J Respir Crit Care Med. 1997;156(2 Pt 2):1-25. https://doi.org/10.1164/ajrccm.156.2.atsstatement.   DOI
4 Koh WJ, Kwon OJ, Lee KS. Diagnosis and treatment of nontuberculous mycobacterial pulmonary diseases: a Korean perspective. J Korean Med Sci. 2005;20:913-25. https://doi.org/10.3346/jkms.2005.20.6.913.   DOI
5 Diriba G, Kebede A, Yaregal Z, Getahun M, Tadesse M, Meaza A, et al. Performance of Mycobacterium Growth Indicator Tube BACTEC 960 with Lowenstein-Jensen method for diagnosis of Mycobacterium tuberculosis at Ethiopian National Tuberculosis Reference Laboratory, Addis Ababa, Ethiopia. BMC Res Notes. 2017;10:181. https://doi.org/10.1186/s13104-017-2497-9.   DOI
6 Gil-Setas A, Mazon A, Alfaro J, Idigoras P. Blood agar, chocolate agar, and Mycobacterium tuberculosis. J Clin Microbiol. 2003;41:4008. https://doi.org/10.1128/JCM.41.8.4008.2003.   DOI
7 Satti L, Ikram A, Abbasi S, Malik N, Mirza IA, Martin A. Evaluation of thin-layer agar 7H11 for the isolation of Mycobacterium tuberculosis complex. Int J Tuberc Lung Dis. 2010;14:1354-1356.
8 Mathur ML, Gaur J, Sharma R, Solanki A. Rapid culture of Mycobacterium tuberculosis on blood agar in resource limited setting. Dan Med Bull. 2009;56:208-210.
9 Drancourt M, Carrieri P, Gevaudan MJ, Raoult D. Blood agar and Mycobacterium tuberculosis: the end of a dogma. J Clin Microbiol. 2003;41:1710-1711. https://doi.org/10.1128/JCM.41.4.1710-1711.2003.   DOI
10 Realini L, De Ridder K, Palomino J, Hirschel B, Portaels F. Microaerophilic conditions promote growth of Mycobacterium genavense. J Clin Microbiol. 1998;36:2565-2570.   DOI
11 Ghodbane R, Raoult D, Drancourt M. Dramatic reduction of culture time of Mycobacterium tuberculosis. Sci Rep. 2014;4:4236. https://doi.org/10.1038/srep04236.   DOI
12 Realini L, De Ridder K, Hirschel B, Portaels F. Blood and charcoal added to acidified agar media promote the growth of Mycobacterium genavense. Diagn Microbiol Infect Dis. 1999;34:45-50. https://doi.org/10.1016/S0732-8893(99)00014-0.   DOI
13 Middlebrook G, Cohn ML. Bacteriology of tuberculosis: laboratory methods. Am J Public Health Nations Health. 1958;48:844-853.   DOI
14 Lee JJ, Suo J, Lin CB, Wang JD, Lin TY, Tsai YC. Comparative evaluation of the BACTEC MGIT 960 system with solid medium for isolation of mycobacteria. Int J Tuberc Lung Dis. 2003;7:569-574.
15 Tenenbein M, Cohen S, Sitar DS. Efficacy of ipecac-induced emesis, orogastric lavage, and activated charcoal for acute drug overdose. An Emerg Med. 1987;16:838-841. https://doi.org/10.1016/S0196-0644(87)80518-8.   DOI
16 Vilcheze C, Hartman T, Weinrick B, Jacobs WR, Jr. Mycobacterium tuberculosis is extraordinarily sensitive to killing by a vitamin C-induced fenton reaction. Nat Commun. 2013;4:1881.https://doi.org/10.1038/ncomms2898.   DOI
17 Palange P, Narang R, Kandi V. Evaluation of culture media for isolation of Mycobacterium species from human clinical specimens. Cureus. 2016;8:E757. https://doi.org/10.7759/cureus.757.
18 Chauhan A, Madiraju MV, Fol M, Lofton H, Maloney E, Reynolds R, et al. Mycobacterium tuberculosis cells growing in macrophages are filamentous and deficient in FtsZ rings. J Bacteriol 2006;188:1856-1865. https://doi.org/10.1128/JB.188.5.1856-1865.2006.   DOI
19 Bashyam MD, Kaushal D, Dasgupta SK, Tyagi AK. A study of mycobacterial transcriptional apparatus: identification of novel features in promoter elements. J Bacteriol. 1996;178:4847-4853.   DOI
20 Winder FG. Mole of action of the antimycobacterial agents and associated aspects of the molecular biology of the mycobacteria, New York: Academic Press; 1982.
21 Hahn MY. Development of growth-enhancing medium containing growth-promoting factors for Mycobacterium tuberculosis culture. Academic research report. Seoul: Yonsei University Industry Academic Cooperation Foundation; 2012. p55.
22 Gazdik MA, McDonough KA. Identification of cyclic AMP-regulated genes in Mycobacterium tuberculosis complex bacteria under low-oxygen conditions. J Bacteriol. 2005;187:2681-2692. https://doi.org/10.1128/JB.187.8.2681-2692.2005.   DOI