• 센서학회지
• /
• 제11권2호
• /
• pp.84-92
• /
• 2002

국소 광적응 기능을 가지는 윤곽검출용 시각칩을 픽셀수 $32{\times}32$의 방사형 구조로 CMOS 공정기술을 이용하여 설계 및 제조하였다. 생체의 망막은 넓은 범위의 입력 광강도에 대해서 물체의 윤곽을 검출할 수 있다. 본 연구에서는 시세포, 수평세포, 쌍극세포로 이루어진 망막의 윤곽검출 기능을 모델링하여 윤곽검출용 인공시각칩을 설계하였다 국소 광적응을 위해 입력 광강도에 따라 수용야의 크기를 국소적으로 바뀌게 하였다. 아울러 단위셀을 방사형으로 배치함으로써 영상데이터의 양을 감소시킴과 동시에 칩의 중심부분으로 갈수록 해상도가 높아지도록 설계하였다. 설계된 칩은 $0.6\;{\mu}m$ double-poly triple-metal 표준 CMOS 공정기술을 이용하여 제조되었으며, HSPICE 시뮬레이션으로 성능을 최적화 시켰다.

• 한국환경농학회지
• /
• 제37권3호
• /
• pp.229-234
• /
• 2018

In vitro 소핵시험(vitMNT)은 유전독성의 유망한 대체시험법 중 하나로, OECD에서 TG로 채택되어 화학물질의 등록에 사용되고 있다. 본 시험에서는 CHL cell을 사용한 vitMN test에서 소핵을 판별하기 위한 최적화된 세포질 조건을 찾고자 하였으며, 양성대조물질로 MMC와 Col을 사용하고 세포 염색을 위해 giemza 용액을 사용하였다. 시험결과, 세포현탁을 위해 사용되는 고정액의 acetic acid의 농도는 1%로 하는 것이 band의 두께와 세포질의 퍼짐성 측면에서 적당하였다. 또한 세포의 깨짐을 최소화하는 최종 고정액의 적하시간은 현탁 후 1~4시간이었다. 이러한 결과는 vitMNT에서 소핵관찰의 신속성, 용이성 및 정확성을 확보하는데 도움이 될 것이다.

• Journal of Photoscience
• /
• 제9권2호
• /
• pp.5-8
• /
• 2002

Photoperiodism is an important adaptive phenomenon in various physiological parameters including reproduction to cope with seasonal changes. Involvement of extraretinal photoreceptors in the photoperiodism in non-mammalian vertebrates has been well established. In addition, circadian clock system is known to be involved in the photoperiodic time measurement. The pathway consists of light-input system, time measurement system (circadian clock), gonadotropin releasing hormone (GnRH) production in the hypothalamus, luteinizing hormone (LH) and follicle stimulating hormone (FSH) production in the pituitary, and final gonadal development. Recently, several laboratories reported photopigments newly cloned in the pineal, eyes and deep brain in addition to already known visual pigments in the retina. These are pinopsin, parapinopsin, VA-opsin, melanopsin, etc. All these photopigments belong to the opsin family having retinal as the chromophore. However, the function of these photopigments remains unknown. I reviewed the studies on the location of the photopigments by immunocytochemistry. I also discussed the results on the action spectra for induction of gonadal development in relation with the location of the photoreceptors. Various physiologically active substances distribute in the vertebrate brain. Such substances are GnRH, GnIH, neuropeptide Y, vasoactive intestinal peptide, c-Fos, galanin, neurosteroids, etc. I summarized the immunhistochemical studies on the distribution and the photoperiodic changes of these substances and discussed the route from the deep brain photoreceptor to GnRH cells.

• JSTS:Journal of Semiconductor Technology and Science
• /
• 제14권5호
• /
• pp.658-665
• /
• 2014

The neural stimulators have been employed to the visual prostheses system based on the functional electrical stimulation (FES). Due to the size limitation of the implantable device, the smaller area of the unit current driver pixel is highly desired for higher resolution current stimulation system. This paper presents a 16-channel compact current-mode neural stimulator IC with digital to analog converter (DAC) sharing scheme for artificial retinal prostheses. The individual pixel circuits in the stimulator IC share a single 6 bit DAC using the sample-and-hold scheme. The DAC sharing scheme enables the simultaneous stimulation on multiple active pixels with a single DAC while maintaining small size and low power. The layout size of the stimulator circuit with the DAC sharing scheme is reduced to be 51.98 %, compared to the conventional scheme. The stimulator IC is designed using standard $0.18{\mu}m$ 1P6M process. The chip size except the I/O cells is $437{\mu}m{\times}501{\mu}m$.

• Environmental Analysis Health and Toxicology
• /
• 제14권1_2호
• /
• pp.1-12
• /
• 1999

Recently, several new methods for the detection of genetic damages in vitro and in vivo based on molecular biological techniques were introduced according to the rapid progress in toxicology combined with cellular and molecular biology. Among these methods, mouse lymphoma thymidine kanase (tk) gene forward mutation assay, single cell gel electrophoresis (comet assay) and transgenic animal and cell line model as a target gene of lac I (Big Blue) and lac Z (Muta Mouse) gene mutation are newly introduced based on molecular toxicological approaches. The mouse lymphoma tk$\^$+/-/ gene assay (MOLY) using L5178Y tk$\^$+/-/ mouse lymphoma cell line is one of the mammalian forward mutation assays, and has many advantages and more sensitive than hprt assay. The target gene of MOLY is a heterozygous tk$\^$+/-/ gene located in 11 chromosome, so it is able to detect the wide range of genetic changes like point mutation, deletion, rearrangement, and mitotic recombination within tk gene or deletion of entire chromosome 11. The comet assay is a rapid, simple, visual and sensitive technique for measuring and analysing DNA breakages in mammalian cells, Also, transgenic animal and cell line models, which have exogenous DNA incorporated into their genome, carry recoverable shuttle vector containing reporter genes to assess endogenous effects or alteration in specific genes related to disease process, are powerful tools to study the mechanism of mutation in vivo and in vitro, respectively. Also in vivo acridine orange supravital staining micronucleus assay by using mouse peripheral reticulocytes was introduced as an alternative of bone marrow micronucleus assay. In this respect, there was an International workshop on genotoxicity procedure (IWGTP) supported by OECD and EMS (Environmental Mutagen Society) at Washington D. C. in March 25-26, 1999. The objective of IWGTP is to harmonize the testing procedures internationally, and to extend to finalization of OECD guideline, and to the agreement of new guidelines under the International Conference of Harmonization (ICH) for these methods mentioned above. Therefore, we introduce and review the principle, detailed procedure, and application of MOLY, comet assay, transgenic mutagenesis assay and supravital staining micronucleus assay.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제38권4호
• /
• pp.221-230
• /
• 2012

Objectives: This study sought to evaluate the efficacy of collagen graft materials, as compared to other graft materials, for use in healing calvarial defects in rabbits. Materials and Methods: Ten mm diameter calvarial defects were made in ten rabbits. The rabbits were then divided into 4 groups: control, autogenous bone graft, SureOss graft, and Teruplug graft. Bone regeneration was evaluated using histological and radiographic methods. Results: Based on visual examination, no distinct healing profile was observed. At 4 weeks after treatment, histological analysis showed there was no bone regeneration in the control group; however, at 8 weeks after treatment, new bone formation was observed around the margin of the defective sites. In the autogenous bone graft group, new bone formation was observed at 4 weeks after treatment and mature bone was detected around the grafted bone after 8 weeks. In the SureOss graft group, at 4 weeks after treatment, acute inflammatory and multinuclear cells were noted around the grafted materials; at 8 weeks after treatment, a decrease in graft materials coupled with new bone formation were observed at the defective sites. In the Teruplug graft group, new bone formation was detected surrounding the bone margin and without signs of inflammation. There were statistically significant differences observed between the graft and control group in terms of bone density as evidenced by radiographic analysis using computed tomography (P<0.05), particularly for the autogenous bone graft group (P<0.001). Conclusion: These results suggested that autogenous bone, SureOss and Teruplug have the ability to induce bone regeneration as compared to an untreated control group. The osteogenic potential of Teruplug was observed to be lower than that of autogenous bone, but similar to that of SureOss.

• Biomolecules & Therapeutics
• /
• 제26권3호
• /
• pp.282-289
• /
• 2018

Melanin is a pigment produced from tyrosine in melanocytes. Although melanin has a protective role against UVB radiation-induced damage, it is also associated with the development of melanoma and darker skin tone. Tyrosinase is a key enzyme in melanin synthesis, which regulates the rate-limiting step during conversion of tyrosine into DOPA and dopaquinone. To develop effective RNA interference therapeutics, we designed a melanin siRNA pool by applying multiple prediction programs to reduce human tyrosinase levels. First, 272 siRNAs passed the target accessibility evaluation using the RNAxs program. Then we selected 34 siRNA sequences with ${\Delta}G{\geq}-34.6kcal/mol$, i-Score value ${\geq}65$, and siRNA scales score ${\leq}30$. siRNAs were designed as 19-bp RNA duplexes with an asymmetric 3' overhang at the 3' end of the antisense strand. We tested if these siRNAs effectively reduced tyrosinase gene expression using qRT-PCR and found that 17 siRNA sequences were more effective than commercially available siRNA. Three siRNAs further tested showed an effective visual color change in MNT-1 human cells without cytotoxic effects, indicating these sequences are anti-melanogenic. Our study revealed that human tyrosinase siRNAs could be efficiently designed using multiple prediction algorithms.

• 전기전자학회논문지
• /
• 제18권4호
• /
• pp.572-580
• /
• 2014

본 연구에서는 교육 및 놀이를 통해 창의력 증진이 가능한 재구성 모듈러 로봇에 대하여 다루고 있다. 보통 모듈러 로봇은 각각 셀들의 조합으로 변형 재구성하고 다양한 형태의 구조를 만드는데 적합한 로봇으로 설계되어 있지만 이를 이용하여 교육과 놀이를 주제로 한 연구는 국외에 소수 있으며 국내에는 전무하다. 제안하는 형태는 기존 모듈러 로봇과 같이 같은 동작만 반복하는 모듈이 아니라 소리를 내는 소리모듈, 바퀴가 달린 바퀴모듈, 시각적 표현을 위한 LED모듈, 전원공급을 위한 전원모듈, 통신을 위한 블루투스모듈, 움직이는 동작을 구현 가능하도록 하는 관절모듈이 있는 구조로 설계되어 있다. 이와 같은 다른 능력이 부여된 각 모듈의 다양한 결합을 이용하여 창의적 활동이 가능하도록 하고, 아이들이 쉽게 사용 할 수 있도록 한다. 본 연구에서는 모듈별 각기 다른 능력을 부여하여 교육과 놀이가 가능하도록 하는 방식의 모듈러 로봇의 설계 및 동작을 다루고 있다. 그리고 연구된 동작은 실제 제작 및 실험을 통해 동작의 유용성을 보였다.

• 한국정보통신학회논문지
• /
• 제17권3호
• /
• pp.533-539
• /
• 2013

최근 의료 및 생체공학 분야의 액추에이터(actuator)와 센서 및 연료전지로 활용할 수 있는 새로운 재료인 IPMC(Ionic Polymer Metal Composite)에 대한 관심이 높아지고 있다. 이러한 IPMC의 특징은 센서(sensor)와 액추에이터(actuator)의 성질을 동시에 가지는 있는 특이한 성질을 가진 복합재료로서 IPMC에 전압을 가하면 움직임이 생기며, IPMC에 움직임이 발생하면 IPMC 내부에 전압이 충전되는 성질이 있어 모션 센서나 힘 센서로도 활용할 수 있다. 본 논문에서는 이러한 IPMC에 대한 몇 가지 특성을 파악하고 IT 기술인 스마트폰의 연계하여 원격지에서 IPMC의 동작을 제어할 수 있도록 시스템을 구성하였다. 또한, 스마트폰을 기반으로 하여 영상정보를 전송하여 모니터링 하도록 하였다. IPMC의 동작제어는 스마트폰의 블루투스를 이용하여 동작명령을 전송하도록 시스템을 구현하였다. 본 논문의 실험 및 구현으로부터 IPMC 물성에 대한 정확한 분석은 부족하지만 향후 IPMC의 활용에 있어서 IT 기술과의 융합을 통하여 센서, 액추에이터, 연료전지 등과 같은 분야에 적극 활용할 수 있음을 확인할 수 있었다.

• 제어로봇시스템학회:학술대회논문집
• /
• 제어로봇시스템학회 2000년도 제15차 학술회의논문집
• /
• pp.103-103
• /
• 2000

In this paper to cope with the reduction of products life-cycle as the variety of products along with the various demands of consumers, a virtual simulator is developed to make the changeover of manufacturing line efficient to embody a virtual simulation similar to a real manufacturing line. The developed virtual simulator can design a layout of a factory and make the time scheduling. Every factory has one simulator so that one product can be manufactured in the factories to use them as virtual factories. We suggest a scheme that heightens the agility to the diversity of manufacturing models by making the information of manufacturing lines and products models to be shared. The developed unit simulator can construct a proper virtual manufacturing line along with the required process of products using several kinds of operator and work cell. A user with the simulator can utilize an interface that makes one to manage the separate task process for each manu(acturing module, change operator components and work cells, and easily teach tasks of each task module. The developed simulator was made for users convenience by Microsoft Visual C++ 6.0 that can develop a program supplying graphic user interface environment and by OpenGL of the Silicon Graphics as a graphic library to embody 3D graphic environment. Also, we show that the simulator can be used efficiently for the agile manufacturing by the communication among the factories being linked by TCP/IP and a hybrid database system made by a hierarchical model and a relational model being developed to standardize the data information.