• Korean Journal of Veterinary Service
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• v.23 no.3
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• pp.271-279
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• 2000

This study was carried out to provide the fundamental information for development of proper vaccination program against infectious bursal disease(IBD) to the local chicken farms. The antigen detection was peformed from 8 samples of bursa of Fabricius with agar gel precipitation(AGP) and indirect immunofluorescent assay(IFA), And also, the antibodies in serum samples were detected by the various serological methods such as commercial ELISA assay, AGP and virus neutralization(VN) test. 1. The antigen detection rates were 25% for AGP which is 2 out of 8 farms and 10 out of 40 bursas, and 25% which Is 2 out of 8 farms and 20% 8 out of 40 bursas for IFA, respectively. 2. The mean titer of maternal antibody (>3,000) existed until 10 days of the age with ELISA-GMT. 3. The antibody positive rates which are over 80% showed until 5 days of the age with ELISA and at 10 days of the age with AGP except one, but none of them showed from 1 day of the age. This report came to conclusions that both the protective maternal antibody titers and the antigen positive rates were significant until at the 10 days of the age.

• Journal of the Korea Institute of Military Science and Technology
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• v.22 no.6
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• pp.763-772
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• 2019

There is a need to use sheath flow nozzle to detect bioaerosol such as virus and bacteria due to their characteristics. In order to enhance the detection performance depending on nozzle parameters, numerical analysis was carried out using a commercial code, ANSYS CFX. Eulerian-lagrangian approach method is used in this simulation. Multiphase flow characteristics between primary fluid and solid were considered. The detection performance was evaluated based on the results of flow field in nozzle chamber such as focusing efficiency and swirl strength. In addition, Latin hypercube sampling(LHS) of design of experiment(DOE) was used for generating a near-random sampling. Then, the acceleration section is optimized using response surface method(RSM). Results show that the optimized model achieved a 6.13 % in a focusing efficiency and 11.47 % increase in swirl strength over the reference model.

• Journal of Korea Multimedia Society
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• v.17 no.10
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• pp.1198-1204
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• 2014

Most of vaccine type security solutions detect intrusion of computer virus or malicious code. However, they almost don't have functionalities of the information leakage detection. In particular, information leakage through keylogging attact cannot be detected. In this paper, we design and implement a solution to detect the leakage of information through keylogging attact. Proposed solution detects the user-specified information in real time. To detect the leakage of user-specified information, the solution extracts the payload field from each outbound packet and compares with user-specified information. We design the solution to reduce the effect on the packet transmission delay time due to packet monitoring operation. And we design a simple user interface. By proposed solution, user can response to intrusion or information leakage immediately because he or she can perceives a leakage of information in real time.

• Journal of Convergence for Information Technology
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• v.11 no.11
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• pp.137-142
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• 2021

Although the internet has gained many conveniences and benefits, it is causing economic and social damage to users due to intelligent malware. Most of the signature-based anti-virus programs are used to detect and defend this, but it is insufficient to prevent malware variants becoming more intelligent. Therefore, we proposes a model that detects and defends the intelligent malware that is pouring out in the paper. The proposed model learns by imaging the characteristics of malware based on deeplearning, and detects newly detected malware variants using the learned model. It was shown that the proposed model detects not only the existing malware but also most of the variants that transform the existing malware.

• International journal of advanced smart convergence
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• v.8 no.2
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• pp.77-87
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• 2019

One of important concerns in information security is to control information flow. It is whether to protect confidential information from being leaked, or to protect trusted information from being tainted. In this paper, we present Piosk (Physical blockage of Information flow Kiosk) that addresses both the problems practically. Piosk can forestall and prevent the leakage of information, and defend inner tangible assets against a variety of malwares as well. When a visitor who carries a re-writable portable storage device, must insert the device into Piosk installed next to the security gate. Then, Piosk scans the device at the very moment, and detects & repairs malicious codes that might be exist. After that, Piosk writes the contents (including sanitized ones) on a new read-only portable device such as a compact disk. By doing so, the leakage of internal information through both insiders and outsiders can be prevented physically. We have designed and prototyped Piosk. The experimental verification of the Piosk prototype implementation reveals that, Piosk can accurately detect every malware at the same detection level as Virus Total and effectively prevent the leakage of internal information. In addition, we compare Piosk with the state-of-the-art methods and describe the special advantages of Piosk over existing methods.

• Analytical Science and Technology
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• v.36 no.6
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• pp.281-290
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• 2023

Zidovudine is an antiretroviral agent prescribed for the prevention and treatment of human immunodeficiency virus/acquired immune deficiency syndrome (HIV/AIDS). It is typically recommended to be used in combination with other antiretroviral drugs. Zidovudine has the potential to generate N-nitrosodimethylamine (NDMA) in the presence of dimethylamine and nitrite salt under acidic reaction conditions during the drug manufacturing process. NDMA is a potent human carcinogen that may be detected in drug substances or drug products. An analytical method was developed to determine NDMA in pharmaceuticals including zidovudine using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The analysis involved reversed-phase chromatography on a Kinetex F5 column with a mobile phase comprising water-acetonitrile mixtures. The detection of positively charged ions was conducted using atmospheric pressure chemical ionization (APCI). The calibration curve demonstrated excellent linearity (r = 0.9997) across the range of 1-50 ng/mL with a highly sensitive limit of detection (LOD) at 0.3 ng/mL. The developed method underwent thorough validation for specificity, linearity, accuracy, precision, robustness, and system suitability. This sensitive and specific analytical method was applied for detecting NDMA in zidovudine drug substance and its formulation currently available in the market, indicating its suitability for drug quality management purposes.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.7 no.2
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• pp.143-152
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• 2004

Purpose: The purpose of this study is to detect viral coproantigens in children who were hospitalized with acute diarrhea and to compare its association with clinical symptoms. Methods: Seventy-four stool samples were collected from children admitted to Ewha Mokdong Hospital from March 1996 to December 1999. The samples were frozen and analyzed for rotavirus, adenovirus, enterovirus, astrovirus, and calicivirus by enzyme immunoassay (EIA) with monoclonal antibody. 53 stool samples were collected from patients with diarrhea (diarrheal group) and 21 stool samples from patients hospitalized for reasons other than diarrhea (control group). Clinical features and laboratory findings were reviewed in both groups. Results: Among 74 stool samples, virus antigens were detected in 60 samples. Of the 60 virus-positive stool samples, 47 enterovirus, 26 rotavirus, 16 adenovirus, 11 astrovirus, and 11 calicivirus antigens were detected by EIA. Of the 60 virus-positive stool samples, 28 samples have one viral antigen, 30 samples have 2 or more viral antigens, and 2 samples showed a simultaneous infection of Salmonella group B and enterovirus. There was no relationship between the detected virus and clinical features. Conclusion: In this study, viral coproantigen and clinical symptoms were not associated. In the future, further larger scale studies are necessary.

• Journal of fish pathology
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• v.20 no.3
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• pp.201-209
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• 2007

Viral hemorrhagic septicemia (VHS) is one of the most serious viral disease of farmed rainbow trout and some marine fishes in Europe and North America. It has been reported in various marine fish species of Asian countries and induced cause mass mortality in Japanese flounder (Paralichthys olivaceus) culturing in Korea. The aims of this study were to monitor VHSV in wild marine fishes and to give critical information for controling the disease through prophylactic methods. Prevalence of the viral disease, geological distribution and reservoir of the virus were investigated using wild marine fishes captured in southern coast and east china sea for two years. (Reverse Transcriptase Polymerase Chain Reaction) RT-PCR results showed that VHSV were detected in 17 (10.6%) out of 160 fish. G gene sequences of viral strains isolated in this study were closely related to that of a reference strain, KVHS01-1, belonging to VHSV genotype Ⅰ. The results suggest that some of wild marine fishes are VHSV carriers and may spread the pathogen directly to fish farmed in coastal area.

• Journal of fish pathology
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• v.23 no.1
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• pp.27-35
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• 2010

Pathogens on the cultured 579 rockfish, Sebastes schlegeli in the marine cage farms from Geoje and Tongyeong of the Southern sea were investigated from 2006 to 2008. The pathogens were detected throughout the year at 46.0~90.0% for 3 years and the detection rate was low with an average 58.1% in May and high with an average 81.5% in October. Bacteria only, bacteria-parasite mix and virus only were found in October and November as well as parasite only, whereas infection of parasite only was dominant in May when the temperature increased and in August when the temperature peaked. Of rockfish, Microcotyle sp. and Caligus sp. were dominant for parasitic disease, and Vibrio sp. and Streptococcus sp. were dominant bacteria. For virus, RSIV and VNNV were detected as dominant organisms. While no virus was detected in 2006, VNNV, VHSV and RSIV were detected in 2007 due to $1.5\sim2.0^{\circ}C$ higher temperature than 2006 in the summer season. For total prevalence by rockfish sizes, the highest was found at 50.0~87.1% in 11~15cm sizes and 50% was found in 30 cm size. Parasite showed a similar trend of 50.0~79.6% as the total prevalence. Prevalence for bacteria varied from 1.6% (for 10 cm group) to 23.8% (for 26 cm group) and higher virus prevalence of 21.5% was found from below 25 cm group.

• Applied Biological Chemistry
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• v.38 no.1
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• pp.49-54
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• 1995

To understand the molecular structure and pathogenesis mechanism of Korean garlic viruses, we have isolated cDNA clones for garlic viruses. The partial nucleotide sequences of 24 cDNA clones were determined and those of five clones containing poly(A) tail were compared with sequences of other plant viruses. One of these clones, V9, has a primary structure similar to the carlavirus group, suggesting that the clone V9 derived from a part of garlic latent virus (GLV). Northern blot analysis with the clone V9 as a probe demonstrated that GLV genome is 8.5 knt long and has a poly(A) tail. The clone V9 encodes coat protein (CP) of 33 kDa and nucleic acid binding protein of 10 kDa in different reading frame. The hexanucleotide motif, 5'-ACCUAA, which is conserved in the 3' noncoding region arid was proposed to be a cis-acting element involved in the production of negative strand genomic RNA was noticed. Complementary sequence to the hexanucleotide motif, 5'-TTAGGT, is also found in the positive strand of V9 RNA. The putative CP gene was cloned into the pRSET-A expression vector and expressed in E. coli BL21. The expressed recombinant V9CP protein was purified by $Ni^{2+}$ NTA affinity chromatography. The anti-V9CP antibody recognizes 34 kDa polypeptide which could be CP of GLV in infected garlic leaf extract. Immunoblot and Northern blot analysis of various cultivars shows wide occurrence of GLV in Korean garlic plants.