• Research in Plant Disease
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• v.13 no.2
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• pp.82-87
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• 2007

The genetic diagnostic method of virion capture (VC)/RT-PCR was developed for the simultaneous detection of three rod shaped viruses of Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus(KGMMV) and Zucchini green mottle mosaic virus (ZGMMV) transmitted by seed in Cucurbit. Out of 12 primer combinations for the three tobamoviruses, a primer set of CGMMV-C724, KGMMV-K513 and ZGMMV-Z407A was useful for mono and triplex VC/RT-PCR. The triplex VC/RT-PCR for the three tobamovirus in Cucurbit could detect specifically without interference among primers and/or plant species of watermelon, gourd, cucumber, melon, pumpkin, squash and Nicotiana benthamiana.

• The Plant Pathology Journal
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• v.22 no.3
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• pp.230-234
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• 2006

Tomato spotted wilt virus (TSWV) has been identified in commercial chrysanthemum cultivars in Korea. Nucleotide sequences of the N gene of TSWV-ch14 isolated from infected chrysanthemum were determined and deposited in GenBank under accession no. DQ453158. The symptoms consisted of dark colored leaf necrosis, black streaks along the stem, wilting of plant parts in 'Sinma'; and chlorotic spots, necrosis of axillary shoots and withering of leaves in 'Hwarang'. Electron micrographs of leaf preparation of Nicotiana rustica infected with TSWV-ch14 contained spherical particles around 85 nm in diameter. TSWV was identified from chrysanthemum by sequence determination of N nucleocapsid protein and virion observation by transmission electron microscope. This is the first reported observation on TSWV in chrysanthemum in Korea.

• Journal of the korean veterinary medical association
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• v.17 no.2
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• pp.33-41
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• 1981

뉴캣슬병 바이러스가 발견된지 50여년이 지난 오늘에도 그 발생은 전 세계적으로 광범위하다. NDV가 분리됨으로 백신개발이 이루어져 1930년대말부터 완전하지는 못했으나 그런대로 방역을 맡았으며 그후 개량발전된 백신으로 각국에서 예방접종하고 있으나 여전히 발생하고 있다. NDV는 Paramyxovirus로서 RNA를 가지고 있으며 크기는 $100\~600{\mu}m$ 범위의 크기와 lipoprotein envelope로 쌓여 있다. 분리동정에 이용되는 혈구응집소, neuraminidase의 작용, 용혈성 등 모두 envelope와 관련이 있으며 이와 관련된 연구가 많이 진행되고 있다. NDV가 세포에 침투하는 과정에서 특이한 receptor에 부착하여 envelope의 용해 및 nucleocapsid의 세포속에 침투 등이 밝혀지고 있으며 NDV의 Virion은 RNA의존 RNA 복합체를 가지고 있고 보족 RNA는 바이러스 단백질 및 RNA를 산생하기 위해서 숙주에 의하여 전환을 한다. 1 일령추의 뇌내접종, 정맥내 접종 및 계태 아치사시간 등의 방법으로 Velogenic, Mesogenic Lentogenic type으로 분류하고 감염력에 따라 Virulent 또는 avirulent로 구분된다. 국내에서 분리된 NDV는 현재 Velogenic형으로 분류되고 있으나 앞으로 지역별, 계절별, 감염된 숙주별로 광범위하게 분리하여 국내에서 유행하고 있는 NDV의 성상조사와 특성을 파악 할 필요성이 요청된다.

• Korean Journal Plant Pathology
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• v.14 no.3
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• pp.223-228
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• 1998

Turnip mosaic virus)TuMV-Ca) was isolated from a Chinese cabbage showing severe mosaic and black necrotic spots symptoms in Korea. The virus was identified as a strain of TuMV by its host range test, particle morphology, serology, double stranded RNA analysis. For detection of the virus, reverse transcription and polymerase chain reaction(RT-PCR) was performed with a set of 18-mer TuMV-specific primers to amplify a 876 bp DNA fragment The virus was rapidly detected from total nucleic acids of virus infected tissues as well as native viral RNA of purified virion particles by RT-PCR. Detection limit of the viral RNA by RT-PCR was 10 fg.

• Biomolecules & Therapeutics
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• v.7 no.2
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• pp.133-137
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• 1999

This study was undertaken to test for anti-Hepatitis B virus (HBV) activity of the aqueous extracts prepared from Eugenia caryophyllate, Ephedra sinica, Cinnamomum cassia. Aqueous extracts were assayed for the inhibition of HBV replication by measurement of HBV DNA and surface antigen (HBsAg) levels in the extracellular medium of HePG2 2.2.15 cells. All extracts decreased the levels of extracellular HBV virion DNA at concentrations ranging from 128 to 256 $\mu$g/ml and inhibited the production of HBsAg dose-dependently. Our findings suggest that these three hebal medicinal plants may have potential to develop as specific anti-HBV drugs in the future.

• Journal of Microbiology
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• v.34 no.4
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• pp.311-315
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• 1996

The retroviral Gag polyprotein directs the assembly of virion particles and plays an important role in some events after entry into a host cell. The Gag polyprotein of a virus mixture is responsible for inducing murine acquired immunodeficiency syndrome (MAIDS) when injected into susceptible strains of mice. In order to identify the host cellular proteins which interact with the MAIDS virus Gag proteins and possibly mediate the function of the Gag proteins, mouse T-cell leukemic cDNA expression library was screened using the yeast GAL4 two hybrid system. Of 11 individual positive clones, the clone Y1 was selected for the study of protein-protein interaction. Its DNA sequence revealed that it was an exact match to the murine SH3 domain-containing protein SH3P8. It is expressed as 2.4 kbp transcripts in testis at higher levels and in various tissues tested at lower levels. Glutathione S-transferase-Y1 fusion protein binds tightly to $Pr60^{def-gag}$ as well as $Pr65^{eco-gag}$.

• YAKHAK HOEJI
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• v.43 no.4
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• pp.458-463
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• 1999

This study was undertaken to test for antiviral activity of the aqueous extracts prepared from 4 medicinal plants of Korea (Terminalia chebula, Sanguisorba officinalia, Rubus coreanus, Rheum palmatum). Aqueous extracts were assayed for the inhibition of hepatitis B virus (HBV) replication by measurement of HBV DNA and surface antigen (HBsAg) levels in the extracellular medium of HepG2 2.2.15 cells. All extracts decreased the levels of extracellular HBV virion DNA at concentrations ranging from 64 to $128{\;}\mu\textrm{g}/ml$ and inhibited the production of HBsAg dose-dependently. Among the 4 tested plants, Terminalia chebula exhibits the most prominent anti-HBV activities. Our findings suggest that these 4 medicinal plants may have potential to develop as specific anti-HBV drugs in the future.

• Journal of the Korean Society of Tobacco Science
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• v.18 no.2
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• pp.101-106
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• 1996

Tobacco mosaic virus (TMV) tomato strain was isolated from tomato "Seo-Kwang" in Korea. The virion was purified by density gradient centrifugation, and total viral RNA was isolated from the purified particles. Coat protein (CP) cDNA of the virus was synthesized by RT-PCR, and the purified cDNA fragment was subcloned to pBluescript II SK-. The analysis of nucleotide sequence showed that this cDNA was 693 nucleotides long from the insert of clone p1571 and p1572 which contain complete codons of the viral coat protein gene (474 nucleotides) and 3' untranslated region. The nucleotides of coat protein encoding cDNA of the strain were 6 nucleotides less than that of TMV common strain isolated from tobacco plant in Korea. The CP gene showed 70% maximum homology with that of the common strain in the nucleotide level and 86% maximum homology in amino acid level.cid level.

• Korean Journal of Microbiology
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• v.29 no.1
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• pp.34-39
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• 1991

The BTV core associated transcriptase activity, assayed by acid precipitable counts, was reduced to an undetectable level after treat the core with .$100{\mu}{\M}$ CDDP. When the RNA transcripts prepared from the CDDP treated BTV core were analysed on agaroseurea gel, it was observed that the band intensity of the large size RNA was reduced while the band intensity of the small size RNA was enhanced. Northern blot analysis showed that much of the small size RNAs appeared to be prematurely terminated transcripts. These results suggest that CDDP adduction to the template RNA blocks chain elongation process of the virion bound transcriptase that is ultimately responsible for the inactivation of BTV infectivity.

• Journal of Microbiology and Biotechnology
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• v.10 no.5
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• pp.714-720
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• 2000

Antigens of Herpes simplex virus type 1 (HSV-1) strain F were immunoblotted to identify the most immunodominant one, and the localization of this antigen was then studied using immunoelectron microscopy. The 67.8 kDa antigen appeared to be the most immunodominant one in a mouse model, and it showed randomly scattered and partially clustered distribution on the surface of the virion. The localization study was performed using immunogold with polyclonal anti-HSV-1 sera produced from BALB/c mice, and immunofluorescence demonstrated that the viral products in the HSV-2 infected Vero cells were distributed throughout the infected host cell, however, mainly on the surface of the host membrane.