• 생약학회지
• /
• 제52권1호
• /
• pp.34-40
• /
• 2021

Proliferation and maintain of dermal papilla during progression of hair-cycle are crucial to the duration of anagen and regulated by diverse signaling pathway such as PI3K/Akt/Wnt/β-catenin pathway. In this study, we investigated the effects and mechanisms of Viola verecunda on dermal papilla cells. Treatment of dermal papilla cells with whole plant extract of V. verecunda resulted in cell proliferation, which was accompanied by up-regulation of cyclin D1, phospho (ser780)-pRB and cdc2 p34, and down-regulation of p27kip1. V. verecunda extract also promoted the levels of phospho (ser473)-Akt and phospho (ser780)-pRB in a time-dependent manner. Inhibition of PI3K/Akt by Wortmannin suppressed progression of cell-cycle, thereby attenuated the increases in proliferation of dermal papilla cells by V. verecunda extract. We further investigated Wnt/β-catenin pathway with respect to the effects of V. verecunda extract on the proliferation of dermal papilla cells. Treatment with V. verecunda extract results in up-regulation of Wnt/β-catenin proteins such as phospho (ser9)-GSKβ, phospho (ser552)-β-catenin and phospho (ser675)-β-catenin. In addition, Wortmannin abrogated V. verecunda extract mediated up-regulation of cdc2 p34 and down-regulation of p27kip1. These finding reveal that the proliferative effect of V. verecunda mediated by alteration of cell-cycle via activating PI3K/Akt/Wnt pathway in dermal papilla cells.

• 한국자원식물학회지
• /
• 제19권6호
• /
• pp.716-720
• /
• 2006

The genetic diversity among the genus Viola was evaluated using the random amplified polymorphic DNA (RAPD) method. A total of 142 distinct amplification fragments by 18 random primers were scored to perform the cluster analysis with UPGMA. Viola species from the subsection Patellares were clustered into group I to IV. The groups from I to IV were consistent with its morphological taxonomy, series Pinnatae, Chinensis, Variegatae, and Patellares in the subsection Patellares, respectively. Even though V. albida and V. albida var. takahasii were classified in Chinensis, they were assigned into group I. The cluster analysis separated other subsections from Patellares in the section Nomimium. Interestingly, V. verecunda and V. grypoceras in subsections Biobatae and Trigonocarpae, respectively, were clustered into group C with a high similarity coefficient. Therefore, RAPD analysis can be used for providing an alternative classification system to identify genotypes and morphological characters of Viola species.

• 한국자원식물학회지
• /
• 제19권6호
• /
• pp.700-705
• /
• 2006

Present studies were performed to determine the physiology of seed germination in Viola species native to Korea. Twelve species, 1 variety and 1 form were collected, classified and used as materials: V. collina, V. blandaefomis, V. rosii, V. chaerophylloides, V. phalacrocarpa, V. patrinii, V. mandshurica, V. mandshurica for. albescence, V. seoulensis, V. yedoensis, V. keiskei, V. variegata, V. variegata var. chinensis, and V. verecunda. V. tricolor 'Helen Mount' was also used to compare wild with cultivated species. In order to investigate the effect of temperature on seed germination, seeds stored at $4{\pm}2^{\circ}C$ for 10 months or 4 years were incubated at 10, 15, 20, $25^{\circ}C$ under 16h illumination with 4 replicates per treatment. Seeds which had not germinated at $10^{\circ}C$ were transferred to $30^{\circ}C$ to assess the effect of temperature change in germination. Germination percent and the days of first, 40% and 80% germination were assessed. Capability of seed germination varied with taxon; Species belonging to subsection Patellares had high ability of germination, compared to species in the other subsections, and series Chinensis was the best among subsection Patellares. Species capable of high germination germinated in all temperatures with reasonably high germination rate, but the other species responded sensitively to temperature with different germination patterns. Higher the temperature, shorter the incubation time required for first, 40% and 80% of germination. Therefore, high temperature was effective in almost all species, not only for inducing high rate of germination but also the uniformity of germination. Temperature change from $10^{\circ}C\;to\;30^{\circ}C$ had a positive effect on seed germination.

• 한국식물병리학회지
• /
• 제14권1호
• /
• pp.52-61
• /
• 1998

This paper is the fifth report about the fungal diseases of economic resource plants observed newly in Korea. It contains short descriptions on symptoms, occurrence conditions, pathogens, and some phytopathological notes for each of 10 fungal plant diseases. They are identified as leaf spot of Adenophora triphylla var. japonica by Septoria lengyelii, leaf spot of Calystegia soldanella by S. convolvuli, leaf spot of Campanula punctata by S. campanulae, leaf spot of Codonopsis lanceolata by S. codonopsidis, leaf spot of Geum japonicum by s. gei, black spot of Oenanthe javanica by s. oenanthes, leaf spot of Oenothera odorata by S. oenotherae, angular leaf spot of Rehmannia glutinosa by S. digitalis, brown spot of Rubus crataegifolius by s. rubi, and leaf spot of Viola verecunda by S. violae-palustris, respectively.

• 한국산림과학회지
• /
• 제109권2호
• /
• pp.124-135
• /
• 2020

오리나무림은 우리나라를 비롯한 동아시아의 습지림을 구성하는 대표적인 임분으로 예전에는 산지, 하변, 배후습지, 그리고 충적 저지 등 다양한 서식환경에서 비교적 흔하게 분포하고 있었으나, 다양한 토지이용과 서식지의 건조화 등의 영향으로 최근에는 보기 드문 식물군락 중의 하나로 여겨지고 있다. 본 연구는 우리나라 산지습지에 자연적으로 분포하고 있는 오리나무림의 군락생태학적 특성을 파악하여 향후 그 체계적 보전 및 관리를 위한 기초자료를 제공하는 데 있다. 전국 66개 지소의 오리나무림을 대상으로 ZM학파의 식물사회학적 방법과 이원지표종분석(TWINSPAN)을 병용하여 군락 유형을 분류한 결과, 모두 8개 단위 유형 (쪽동백나무형, 들메나무-병꽃나무형, 참조팝나무형, 콩제비꽃형, 꼬리조팝나무-물봉선형, 왕미꾸리광이형, 진퍼리새형, 그리고 상수리나무-생강나무형)이 구분되었으며, 2개 군락, 4개 아군락, 그리고 8개 변군락(variant)의 식생단위 체계로 구성되어 있음을 알 수 있었다. 또한, 군락유형별 생태적 특성(구성종의 총피도(/100㎡), 중요치, 상재도, 생활형 조성, 종다양성 지수, 군락유사도 그리고 지표종 등)을 비교하였다.

• The Korean Journal of Ecology
• /
• 제22권5호
• /
• pp.277-285
• /
• 1999

도시의 식재림을 자연림으로 복원하기 위한 기초조사로서 서울시 강남구 청담동에 위치한 청담근린공원 내에서 교목층을 구성하고 있는 은수원사시나무를 벌목한 후 초기 2년간 식물상, 식생, 목본식 물의 생장량 변화를 조사하였다. 그 결과 다음과 같았다. 첫째, 벌목의 결과 목본식물종의 변화는 없었으나 초본식물은 1년 후 콩제비꽃 등 3종이 사라지고 15종이 새로 출현하여 종 수가 44% 증가하였다. 새로 침입한 종은 대부분 박주가리 등의 교란지종이거나 붉은서나물 등의 귀화종이었다. 둘째, 벌목은 기존의 아교목층이나 관목층을 구성하고 있던 갈참나무, 신갈나무, 상수리나무, 팥배나무 둥의 피도를 급격히 증가시켰다. 싸리의 피도는 주변의 다른 식물에 따라 변화가 심하였다. 셋째, 초본층의 구성종 중 맑은대쑥의 피도 증가가 가장 뚜렷하였으며 이외의 종의 것은 큰 변화가 없었다. 넷째, 참나무류는 피음이 제거되면 생장이 상당히 증가되었다. 실험구에서는 벌목 후 다음해의 DBH 증가는 갈참나무, 신갈나무 및 떡갈나무에서 각각 53.0%, 22.9% 및 28.8%, 대조구에서는 각각 23.1%, 8.3% 및 6.1%이었다. 그리고 가지의 생장량은 벌목 전해를 기준으로 벌목 다음해 실험구의 갈참나무, 신갈나무 및 떡갈나무에서 각각 565%, 191%, 644%이었고, 대조구의 것에서 각각 117%, 100%, 42%이었다. 그런데 갈참나무와 떡갈나무는 상대적으로 줄기보다 가지의 생장 증가가 뚜렷하였지만 신갈나무는 그 반대이었다.