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http://dx.doi.org/10.22889/KJP.2021.52.1.34

The Mechanism of Whole Plant Extract of Viola verecunda on the Proliferation of Dermal Papilla Cells  

Kang, Jung-Il (Department of Medicine, School of Medicine, Jeju National University)
Seo, Min Jeong (Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resourecs)
Choi, Youn Kyung (Department of Medicine, School of Medicine, Jeju National University)
Shin, Su Young (Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resourecs)
Hwang, Yong (Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resourecs)
Goh, Jae duk (Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resourecs)
Yoo, Eun-Sook (Department of Medicine, School of Medicine, Jeju National University)
Kim, Sang-Cheol (Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resourecs)
Kang, Hee-Kyoung (Department of Medicine, School of Medicine, Jeju National University)
Publication Information
Korean Journal of Pharmacognosy / v.52, no.1, 2021 , pp. 34-40 More about this Journal
Abstract
Proliferation and maintain of dermal papilla during progression of hair-cycle are crucial to the duration of anagen and regulated by diverse signaling pathway such as PI3K/Akt/Wnt/β-catenin pathway. In this study, we investigated the effects and mechanisms of Viola verecunda on dermal papilla cells. Treatment of dermal papilla cells with whole plant extract of V. verecunda resulted in cell proliferation, which was accompanied by up-regulation of cyclin D1, phospho (ser780)-pRB and cdc2 p34, and down-regulation of p27kip1. V. verecunda extract also promoted the levels of phospho (ser473)-Akt and phospho (ser780)-pRB in a time-dependent manner. Inhibition of PI3K/Akt by Wortmannin suppressed progression of cell-cycle, thereby attenuated the increases in proliferation of dermal papilla cells by V. verecunda extract. We further investigated Wnt/β-catenin pathway with respect to the effects of V. verecunda extract on the proliferation of dermal papilla cells. Treatment with V. verecunda extract results in up-regulation of Wnt/β-catenin proteins such as phospho (ser9)-GSKβ, phospho (ser552)-β-catenin and phospho (ser675)-β-catenin. In addition, Wortmannin abrogated V. verecunda extract mediated up-regulation of cdc2 p34 and down-regulation of p27kip1. These finding reveal that the proliferative effect of V. verecunda mediated by alteration of cell-cycle via activating PI3K/Akt/Wnt pathway in dermal papilla cells.
Keywords
Viola verecunda; Dermal papilla cells; PI3K/Akt; Wnt/${\beta}$-catenin;
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