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Microencapsulation of Lactobacillus plantarum MB001 and its probiotic effect on growth performance, cecal microbiome and gut integrity of broiler chickens in a tropical climate

  • Sasi Vimon;Kris Angkanaporn;Chackrit Nuengjamnong
    • Animal Bioscience
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    • v.36 no.8
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    • pp.1252-1262
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    • 2023
  • Objective: Microencapsulation technologies have been developed and successfully applied to protect the probiotic bacterial cells damaged by environmental exposure. This study aimed to investigate the effects of microencapsulation of Lactobacillus plantarum MB001 on the growth performance, ileal nutrient digestibility, jejunal histomorphology and cecal microbiome of broiler chickens in a tropical climate. Methods: A total of 288 one-day-old female broilers (Ross 308) were randomly allocated into 4 groups (6 replicates of 12 birds). Treatments included, i) a basal diet (NC), ii) NC + avilamycin (10 mg/kg) (PC), iii) NC + non-encapsulated L. plantarum MB001 (1×108 colony-forming unit [CFU]/kg of diet) (N-LP), iv) NC + microencapsulated L. plantarum MB001 (1×108 CFU/kg of diet) (ME-LP). Results: Dietary supplementation of ME-LP improved average daily gain, and feed conversion ratio of broilers throughout the 42-d trial period (p<0.05), whereas ME-LP did not affect average daily feed intake compared with NC group. Both N-LP and ME-LP improved apparent ileal digestibility of crude protein and ether extract compared with NC group (p<0.05). The broilers fed ME-LP supplemented diet exhibited a beneficial effect on jejunal histomorphology of villus height (VH), crypt depth (CD) and villus height to crypt depth ratio (VH:CD) of broilers compared to NC group (p<0.05). At the phylum level, Firmicutes was enriched (p<0.05) and Proteobacteria was decreased (p<0.05) only in the ME-LP group. At the genus level, the ME-LP diets increased (p<0.05) the number of both Lactobacillus and Enterococcus compared to NC, PC, and N-LP groups (p<0.05). Conclusion: Microencapsulation assists the efficient functioning of probiotics. ME-LP could be potentially used as a feed additive for improvement of cecal microbiota, gut integrity and nutrient utilization, leading to better performance of broilers.

Soil moisture estimation of YongdamDam watershed using vegetation index from Sentinel-1 and -2 satellite images (Sentinel-1 및 Sentinel-2 위성영상기반 식생지수를 활용한 용담댐 유역의 토양수분 산정)

  • Son, Moobeen;Chung, Jeehun;Lee, Yonggwan;Woo, Soyoung;Kim, Seongjoon
    • Proceedings of the Korea Water Resources Association Conference
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    • 2021.06a
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    • pp.161-161
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    • 2021
  • 본 연구에서는 금강 상류의 용담댐 유역(930.0 km2)을 대상으로 Sentinel-1 SAR(Synthetic Aperture Radar) 및 Sentinel-2 MultiSpectral Instrument(MSI) 위성영상을 활용한 토양수분 산출연구를 수행하였다. 연구에 사용된 자료는 10 m 해상도의 Sentinel-1 IW(Interferometric Wide swath) mode GRD(Ground Range Detected) product의 VV(Vertical transmit-Vertical receive) 및 VH(Vertical transmit-Horizontal receive) 편파자료와 Sentinel-2 Level-2A Bottom of Atmosphere(BOA) reflectance 자료를 2019년에 대해 각 6일 및 5일 간격으로 구축하였다. 위성영상의 Image processing은 SNAP(SentiNel Application Platform)을 활용하여 Sentinel-1 영상의 편파 별(VV, VH) 후방산란계수와 Sentinel-2의 적색(Band-4) 및 근적외(Band-8) 영상을 생성하였다. 토양수분 산출 모형은 다중선형회귀모형(Multiple Linear Regression Model)을 활용하였으며, 각 지점에 해당하는 토양 속성별로 모형을 생성하였다. 모형의 입력자료는 Sentinel-1 위성의 편파별 후방산란계수, Sentinel-1 위성에서 산출된 식생지수 RVI(Radar Vegetation Index)와 Sentinel-2 위성에서 산출된 NDVI(Normalized Difference Vegetation Index)를 활용하여 식생의 영향을 반영하고자 하였다. 모의 된 토양수분을 검증하기 위해 6개 지점의 TDR(Time Domain Reflectometry) 기반 실측 토양수분 자료를 수집하고, 상관계수(Correlation Coefficient, R), 평균제곱근오차(Root Mean Square Error, RMSE) 및 IOA(Index of Agreement)를 활용하여 전체 기간 및 계절별로 나누어 검증할 예정이다.

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Effect of enzymolytic soybean meal supplementation on performance, nitrogen excretion, serum biochemical parameters and intestinal morphology in broilers fed low-protein diets

  • Xin Zhu;Kai Gao;Ziyi Zhang;Haiying Liu;Guiqin Yang
    • Animal Bioscience
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    • v.36 no.11
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    • pp.1718-1726
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    • 2023
  • Objective: The objective of this study was to investigate the effect of supplementation with enzymolytic soybean meal (ESBM) on broilers fed low crude protein (CP) diets. Methods: A total of 360 one-day-old broilers were randomly assigned to six treatments with 6 replicates per treatment and 10 chicks per replicate for a period of 42 days. Chicks were fed a basal standard high-CP diet as a positive control (PC), a low-CP diet (reducing 10 g/kg CP from the PC) as a negative control (NC), or an NC + 0.5%, 1.0%, 1.5%, or 2.0% ESBM diet. Results: Compared to chicks fed the PC, chicks fed the NC had a decreased body weight gain (BWG, p<0.05) from 1 to 42 days, but supplementation with 2.0% ESBM restored BWG (p<0.05) and even linearly improved the feed conversion rate (FCR, p<0.05). Digestibility of CP and ether extract was increased (p<0.05) in chicks fed a 1.0% ESBM diet compared to the PC. With increasing levels of ESBM, nitrogen (N) excretion decreased (p<0.05). The addition of ESBM to the diet did not affect (p>0.05) serum concentrations of total protein, albumin and total cholesterol but led to a descending trend in triglycerides and an ascending trend in calcium and urea N at 42 days (p<0.10). There were no differences (p>0.05) in villus height (VH), crypt depth (CD), and VH/CD (V/C) of the duodenum and jejunum between the PC and NC at both 21 days and 42 days, while increasing dietary ESBM levels linearly (p<0.05) decreased CD and increased V/C of the duodenum and jejunum at both 21 days and 42 days. Conclusion: The findings indicated that ESBM could be used in broiler low-CP diets to improve production performance, decrease N excretion, and enhance intestinal health.

Analysis of Offshore Aquaculture Detection Techniques Using Synthetic Aperture Radar Images (레이더 영상을 이용한 연안 양식장 탐지 기법 분석)

  • Do-Hyun Hwang;Hahn Chul Jung
    • Korean Journal of Remote Sensing
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    • v.39 no.6_1
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    • pp.1401-1411
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    • 2023
  • In the face of escalating utilization of the marine spatial domain, conflicts have emerged among stakeholders, necessitating effective management strategies beyond conventional government permits and regulations. Particularly within the domain of aquaculture, operational oversight relies on a localized licensing system, posing challenges in accurately assessing the prevailing circumstances. This research employs synthetic aperture radar (SAR) imagery as a tool to monitor coastal aquaculture fish farms, aimed at enhancing insights into management protocols. Leveraging Sentinel-1A imagery and time series SAR data integration, a superimposition technique is utilized, facilitating noise reduction while retaining crucial information regarding smaller-scale facilities, such as fish farms. Through analysis of VH polarization data, a detection overall accuracy of approximately 88% for coastal fish farms was achieved. The findings of this study offer potential applications in the continuous monitoring of aquaculture farms in correspondence with seasonal variations in aquaculture yields, thereby proposing frameworks for the establishment of effective management cycles for marine space utilization.

Effects of Dietary Bacillus subtilis and Oregano Oil Supplementation on Performance, Egg Quality, and Intestinal Morphology in Late-Phase Laying Hens (산란말기 사료 내 Bacillus subtilis와 오레가노 오일 첨가가 산란계의 계란 생산성, 계란품질 및 장의 형태학적 특성에 미치는 영향)

  • Hyunsoo Kim;Hee-Jin Kim;Yeon-Seo Yun;Woo-Do Lee;Hyekyoung Shin;Jiseon Son;Eui-Chul Hong;Ik Soo Jeon;Hwan-Ku Kang
    • Korean Journal of Poultry Science
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    • v.50 no.4
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    • pp.311-323
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    • 2023
  • This study aimed to investigate the impact of Bacillus subtilis-based probiotics and oregano essential oil on the production performance, egg quality, and intestinal morphology of late-phase laying hens (69-weeks). A total of 150 laying hens of 69-week-old were randomly allotted into 6 treatment groups with 5 replicates of 5 birds in each replicate. Laying hens were divided into high (H) and average (A) egg production groups prior to the trial. The hens in each group were supplemented with Bacillus subtilis, or oregano essential oil: CON, a basal diet; BS, basal diet plus 3 × 108 CFU/kg feed Bacillus subtilis; OEO, basal diet plus 0.3 g/kg feed oregano essential oil. Egg performance, blood characteristics, egg quality, and intestinal morphology of the late-phase laying hens were evaluated. Both BS and OEO significantly enhanced (P<0.05) egg production compared to CON in high egg production. The blood characteristics indicated no significant differences based on the egg production and the supplementation of BS and OEO in the late-phase laying hens. The eggshell strength was significantly improved (P<0.05) in both OEO compared to both CON. A significantly decreased (P<0.05) the villus height to crypt depth ratio (VH/CD) in the ileum compared to H, and in the treatment groups supplemented with BS and OEO, VH/CD showed a significant increase (P<0.05) compared to both CON. These results suggest that the supplementation of Bacillus subtilis and oregano essential oil in the diet of late-phase laying hens could serve as a potential strategy to enhance egg production, egg quality, and gut health.

Measurements of Microwave Polarimetric Backscattering from a Wet Soil Surface and Comparison with a Semi-empirical Scattering Model

  • Oh, Yi-Sok
    • Proceedings of the KSRS Conference
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    • 1999.11a
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    • pp.154-157
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    • 1999
  • Microwave polarimetric backscattering from a wet soil surface had been measured using a Ku-band polarimetric scatterometer at the incidence angles ranging from 10$^{\circ}$ to 70$^{\circ}$ Since the accurate target parameters as well as the radar parameters are necessary for radar scattering modeling, a complete and accurate set of ground truth data were also collected, from which accurate measurements were made of the rms height, correlation length, and dielectric constant. The measured polarimetric backscattering coefficients (vv-, hh-, vh-, hv-polarizations) were compared with theoretical models and empirical models. A new semi-empirical model for microwave polarimetric radar backscattering from bare soil surfaces was developed using polarimetric radar measurements and the knowledge based on the theoretical and numerical solutions. The model was found to yield very good agreement with the backscattering measurements of this study.

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RETRIEVAL OF SOIL MOISTURE AND SURFACE ROUGHNESS FROM POLARIMETRIC SAR IMAGES OF VEGETATED SURFACES

  • Oh, Yi-Sok;Yoon, Ji-Hyung
    • Proceedings of the KSRS Conference
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    • 2008.10a
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    • pp.33-36
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    • 2008
  • This paper presents soil moisture retrieval from measured polarimetric backscattering coefficients of a vegetated surface. Based on the analysis of the quite complicate first-order radiative transfer scattering model for vegetated surfaces, a simplified scattering model is proposed for an inversion algorithm. Extraction of the surface-scatter component from the total scattering of a vegetation canopy is addressed using the simplified model, and also using the three-component decomposition technique. The backscattering coefficients are measured with a polarimetric L-band scatterometer during two months. At the same time, the biomasses, leaf moisture contents, and soil moisture contents are also measured. Then the measurement data are used to estimate the model parameters for vv-, hh-, and vh-polarizations. The scattering model for tall-grass-covered surfaces is inverted to retrieve the soil moisture content from the measurements using a genetic algorithm. The retrieved soil moisture contents agree quite well with the in-situ measured soil moisture data.

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Comparative evaluation of intracranial aneurysms between 3D-TOF MRA with 3.0T MR and CTA with 16-slices MDCT

  • 정태섭;이재훈;정우석;조은석
    • Proceedings of the KSMRM Conference
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    • 2003.10a
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    • pp.23-23
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    • 2003
  • To evaluate clinical visualization of intracranial aneurysms between 3D-TOF MRA with 3.0T MR and CTA with 16-slices MDCT. In a prospective series, 12 patients with 16 aneurysms were studies with 3D-TOF MRA and CTA. MRA were performed on a GE Signa 3.0 T system (Signa VH/i, GE) with 25/3/20 (TR/TE/FA). CTA were peformed on a 16 slice MDCT (Sensation 16, Somatom, Siemens) with IV shooting of 80 ml iodinated contrast mediumat antecubital vein at a rate of 3.5 ml/sec. Four among 12 patients underwent DSA for surgery. Size, shape, neck and parent vessel of aneurysms were evaluated for comparison of visualization and detectability of aneurysms.

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Purification and Characterization of Invertase from Korean Ginseng Panax ginseng (고려인삼(Panax ginseng) Invertase의 정제와 그 특성)

  • 김용환;김병묵
    • Journal of Ginseng Research
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    • v.14 no.1
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    • pp.14-20
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    • 1990
  • In An invertase (EC 3.2.1.26) was extracted from Korean giseng (Panax ginseng C.A. Meyer) with distilled tvater The ginseng invertase was purified about 62.6 folds purified by procedures including ammonium sulfate fractionation , DEAE-cellulofine chromatography and gelfiltrations through Sephadex G-75 and the recovery of enzyme activity was 11.1%. The homogeneity of the purified enzyme was probed by polyacrylamide gel disc electrophoresis. The purifled enzyme was divided into two different subunits by treating with a mixture of SDS and 2-mercautoethanol, and the molecular weight of the large subunit was estimatedtobe 116,000 and that of the small one to be 14,000. The optimal VH and temperature of the enzyme were pH 6 and 45$^{\circ}C$, respectively. The enzyme hydrolyzed specifically the hydrolyzation of the -fructofuranosides such as sucrose, raffinose and inulin. The Km values of the enzyme for sucrose and raffinose were determined to be 0.85 and 0.6 mM, respectively.

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A Semi-empirical Model for Polarimetric Radar Backscattering from Bare Soil Surfaces

  • Oh, Yi-Sok
    • Korean Journal of Remote Sensing
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    • v.17 no.2
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    • pp.141-153
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    • 2001
  • A semi-empirical polarimetric backscattering model for bare soil surfaces is presented. Based on measurements by using polarimetric scatterometers and the JPL AirSAR, as well as the theoretical models, the backscattering coefficints $\sigma$$^0_w$, $\sigma$$^0_{hh}$ and $\sigma$$^0_{vh}$, and the parameters of the copolarized phase-difference probability density function, namely the degree of correlation $\alpha$ and the copolarized-phase-difference $\zeta$, are modeled empirically in terms of the volumetric soil moisture content m$_v$ and the surface roughness parameters $k_s$ and $k_l$, where k=2$\pi$f/c, s is the rms height and l is the correlation length.