• Title/Summary/Keyword: Veterinary Science

Search Result 7,041, Processing Time 0.032 seconds

Isolation and identification of Vibrio harveyi from chub mackerel (Scomber japonicus)

  • Lee, Young-Ran;Jun, Jin-Woo;Giri, Sib Sankar;Kim, Hyoun-Joong;Yun, Sae-Kil;Chi, Cheng;Kim, Sang-Guen;Koh, Jeong-Rack;Jung, Ji-Yun;Park, Se Chang
    • Korean Journal of Veterinary Research
    • /
    • v.56 no.1
    • /
    • pp.45-46
    • /
    • 2016
  • For several days, there was a series of mortalities of chub mackerel (Scomber japonicus) that were reared for public exhibition in a private aquarium in Seoul, Korea. As part of the diagnosis of the dead fish, a bacterial isolate from the kidney was cultured, identified, and confirmed to be Vibrio (V.) harveyi using Vitek System 2 and 16S rRNA gene sequencing. Phylogenetic analysis was also performed by the neighbor-joining method. As a result, the V. harveyi isolated from chub mackerels of a private aquarium in Korea, called as SNUVh-LW1, was clustered in the same group with V. harveyi ATCC33843.

Gene Clustering for Reuterin Production in the Probiotic Bacterium Lactobacillus reuteri $JCMll12^T$ and its Functional Importance for Viability in the Mammalian Gastrointestinal Tract

  • Morita, Hidetoshi;Masaoka, Toshio;Horikawa, Hiroshi;Oshima, Kenshiro;Suzuki, Takehito;Murakami, Masaru;Kato, Yukio;Hisamatsu, Shin;Takizawa, Tatsuya;Kanemaki, Nobuyuki;Saito, Yasuhide;Naito, Hitoyuki;Kishikawa, Seigou;Nishita, Toshiho;Fukuyama, Masafumi;Sakata, Ryoichi;Arishima, Kazuyoshi;Kiuchi, Akio;Wada, Tomonori;Fukuoka, Hideo;Shino, Masao;Chinone, Shiro;Shiba, Tadayoshi;Yoshimura, Tetsuhiko;Hattori, Masahira
    • Proceedings of the Korean Society for Applied Microbiology Conference
    • /
    • 2004.06a
    • /
    • pp.127-132
    • /
    • 2004
  • PDF

Molecular detection of Aeromonas hydrophila isolated from albino catfish, Clarias sp. reared in an indoor commercial aquarium

  • Choresca, Casiano H. Jr.;Gomez, Dennis K.;Han, Jee-Eun;Shin, Sang-Phil;Kim, Ji-Hyung;Jun, Jin-Woo;Park, Se-Chang
    • Korean Journal of Veterinary Research
    • /
    • v.50 no.4
    • /
    • pp.331-333
    • /
    • 2010
  • Moribund albino catfish, Clarias sp., displayed from an indoor private commercial aquarium were submitted in the laboratory for diagnostic examination. Dense culture of bacteria was recovered from the kidney and was characterized using Vitek System 2 and showed 98% probability to Aeromonas (A.) hydrophila. PCR result showed positive using A. hydrophila extracellular hemolysin gene ahh1 (130 bp) and aerolysin gene aerA (309 bp). The 16S rRNA gene was identical and exhibited 97% sequence similarity with the other known isolates of A. hydrophila available in the GenBank. In this paper, we reported the isolation and molecular detection of A. hydrophila from an albino catfish.

Cluster Analysis of 12 Chinese Native Chicken Populations Using Microsatellite Markers

  • Chen, G.H.;Wu, X.S.;Wang, D.Q.;Qin, J.;Wu, S.L.;Zhou, Q.L.;Xie, F.;Cheng, R.;Xu, Q.;Liu, B.;Zhang, X.Y.;Olowofeso, O.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.17 no.8
    • /
    • pp.1047-1052
    • /
    • 2004
  • The genomes of Chinese native chicken populations were screened using microsatellites as molecular markers. A total of, 528 individuals comprisede12 Chinese native chicken populations were typed for 7 microsatellite markers covering 5 linkage groups and genetic variations and genetic distances were also determined. In the 7 microsatellite loci, the number of alleles ranged from 2 to 7 per locus and the mean number of alleles was 4.6 per locus. By using fuzzy cluster, 12 Chinese native chicken populations were divided into three clusters. The first cluster comprised Taihe Silkies, Henan Game Chicken, Langshan Chicken, Dagu Chicken, Xiaoshan Chicken, Beijing Fatty Chicken and Luyuan Chicken. The second cluster included Chahua Chicken, Tibetan Chicken, Xianju Chicken and Baier Chicken. Gushi Chicken formed a separate cluster and demonstrated a long distance when comparing with other chicken populations.

A comparison of single dose efficacy of Mycoplasma hyopneumoniae bacterin in swine farms with different serological patterns of PRRSV and PCV2

  • Kim, Hye Kwon;Moon, Hyoung Joon;Kim, Eun Mi;Yang, Jeong Sun;Pakr, Seong Jun;Luo, Yuzi;Lee, Chul Seung;Song, Dae Sub;Kang, Bo Kyu;Lee, Jaebum;Park, Bong Kyun
    • Korean Journal of Veterinary Research
    • /
    • v.48 no.3
    • /
    • pp.267-274
    • /
    • 2008
  • This study was to evaluate the efficacy of single dose Mycoplasma hyopneumoniae (M. hyo)-vaccination in the swine farms which had different serological patterns of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2). A minimum of 240 pigs from each farm was applied, allocating M. hyo vaccinated and control groups. The PRRSV and PCV2 infections were analyzed by serological method (commercial ELISA kit). After administrating pigs a single dose of M. hyo vaccine or control saline at 3 weeks of age, serum antibodies to M. hyo, PRRSV and PCV2 were monitored at 4, 10, 16 and 22 weeks of age. Mortality, weight changes, feed conversion ratio (FCR) and lung score were also evaluated. A single-dose vaccination of M. hyo bacterin was efficacious to reduce mycoplasmal lung lesions and induce good humoral immune response. However, FCR was improved only in one of the three farms where showed seronegative status to both PRRSV and PCV2 in the period from 4 to 16 weeks of age. These results might imply that M. hyo vaccine alone could not overcome the PRRSV and PCV2 infection-associated wasting in the field condition. Therefore, the control of PRRSV and PCV2 should be considered to obtain the better effects of M. hyo vaccination.

Primary diffuse hepatocellular carcinoma in a captive polar bear (Ursus maritimus)

  • Krisdianti, Krisdianti;Kim, Taeyeon;Aufa, Sulhi;Lee, Sangjoon;Ahn, Sangjin;Cho, Ho-Seong;Oh, Yeonsu
    • Korean Journal of Veterinary Service
    • /
    • v.44 no.4
    • /
    • pp.299-303
    • /
    • 2021
  • A captive male polar bear (Ursus maritimus) was found dead after a long-term anorexia at a Park Zoo in Republic of Korea. At necropsy, the liver lesion was prominent with hepatomegaly carrying a big mass and numerous small nodules in various sizes. The cut surface of the nodules was creamy white, firm, and solid with umbilicated appearance. Histologically, there was proliferation of hepatocellular neoplastic cells arranged in glandular-like (pseudoglandular) pattern and complete loss of hepatocellular arrangement. The immunoreactivity for neoplastic hepatocyte antigens was positive for hepatocyte paraffin 1, and negative for vimentin, cytokeratin 7 and 19, and carcinoembryonic antigen. Based on those diagnostic features, the neoplasia was diagnosed as hepatocellular carcinoma. To the author's knowledge, this is the first case report of hepatocellular carcinoma in the captive polar bear in Republic of Korea.

Tumor-like lymphoplasmacytic conjunctivitis in the third eyelid in a dog

  • Hyeonu Sung;Jisoo Park;Jury Kim;Seonmi Kang;Gillian C. Shaw;Kangmoon Seo
    • Journal of Veterinary Science
    • /
    • v.25 no.1
    • /
    • pp.16.1-16.7
    • /
    • 2024
  • This report aims to describe a case of tumor-like lymphoplasmacytic conjunctivitis in a 7-year-old spayed-female Pomeranian. On complete ophthalmic examination, a mass with papillary projections was noted on the bulbar surface of the right third eyelid. Debulking of the mass was performed while preserving as much of the third eyelid as possible. On the histopathological examination, the mass was diagnosed as lymphoplasmacytic conjunctivitis with mild epithelial hyperplasia. Although a slight regrowth of the mass was noted 3 weeks after surgery, intralesional injection of triamcinolone acetonide led to its disappearance. There was no further recurrence after 5 months.

Marsupialization of the Nictitating Membrane Cyst Following Cherry Eye Repair in a Dog

  • Kim, Sunhyo;Kang, Seon-mi;Susanti, Lina;Kim, Boyun;Park, Yoonji;Shim, Jaeho;Go, Seokmin;Lee, Eunji;Seo, Kangmoon
    • Journal of Veterinary Clinics
    • /
    • v.37 no.3
    • /
    • pp.149-152
    • /
    • 2020
  • One-year-old male Cocker Spaniel dog was referred for the third eyelid enlargement and inflammation in the left eye (OS). It gradually swelled for 2 weeks after the cherry eye repair by conjunctival mucosa pocket procedure at a private animal clinic. Routine ophthalmic examinations including neuro-ophthalmic examination, Schirmer tear test, intraocular pressure and corneal fluorescein staining were all normal. No lesions were found on slit lamp biomicroscopy and indirect ophthalmoscopy except for third eyelid swelling in the OS. Ultrasonography revealed cystic structure within the OS nictitating membrane. Fluid from the cyst was aspirated and there were no microorganisms or neoplastic changes. Surgical intervention was performed under general anesthesia. On the day of the surgery, there was a deep corneal ulcer in the OS, which had not existed before. Ventral palpebral surface of the third eyelid was incised horizontally to the shaft of the T-shaped hyaline cartilage. And then, a full thickness of the cystic wall was incised and marsupialized. Additionally, a direct suture was performed on the ulcerated cornea. Topical and systemic antibiotics and anti-inflammatory drugs were prescribed. One month after the surgery, the third eyelid swelling and the discharge were improved. Marsupialization of the nictitating membrane cyst relieved the swelling of the third eyelid and inflammation. It could be a simple but effective surgical intervention for the cystic complication of conjunctival mucosa pocket procedure in dogs.