• Journal of Korean Society of Environmental Engineers
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• v.28 no.12
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• pp.1287-1292
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• 2006

Constructed wetlands are well known as highly efficient system to treat wastewater from different sources. Among the constructed wetlands, upflow types of constructed wetlands have become a common selection of wastewater during the last decade. We conducted a pilot scale study at peen house on treating potential of nutrients by upflow vegetated filter(UVF) pilot wetland which was combined with hydrodynamic separator and used the cattail plant(Typha angustifolia), and operated with artificial nutrients influent. This study evaluate the performances of upflow vegetated fille, in removal of nutrients. The objectives of this study were two-fold: (i) to evaluate the nutrients removal performance of pilot-scale upflow vegetated filter, filled with a mixture of perlite and soil media and planted with cattails and (ii) to design of scale-up upflow vegetated filter using Froude number. Results indicated that, under the condition of the ranges of hydraulic surface load rate were $22.7{\pm}9.6\;m^3/m^2/day$, the average removal of $COD_{Mn}$, and TN, TP were 57.5%, 40.0% and 41.5%, respectively. Computational fluid dynamics, FLUENT 6.0 program was used to predict the distribution of velocity in UVF and hydrodynamic separator. Full scale UVF was designed using the Froude number scale-up method that was assumed geomertic similarity between model and prototype. Result shows that the UVF with 3 m diameter has capacity of design sewage flowrate 75 $m^3/day$.

• Journal of Microbiology and Biotechnology
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• v.25 no.7
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• pp.999-1006
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• 2015

The endo-polygalacturonase gene (endo-pgaA) was cloned from DNA of Aspergillus niger SC323 using the cDNA synthesized by overlapping PCR, and successfully expressed in Saccharomyces cerevisiae EBY100 through fusing the α-factor signal peptide of yeast. The fulllength cDNA consists of 1,113 bp and encodes a protein of 370 amino acids with a calculated molecular mass of 38.8 kDa. After induction by galactose for 48 h, the activity of recombinant endo-PgaA in the culture supernatant can reach up to 1,448.48 U/mg. The recombinant protein was purified to homogeneity by ammonium sulfate precipitation and gel filtration column chromatography and subsequently characterized. The optimal pH and temperature of the purified recombinant enzyme were 5.0 and 50℃, respectively. The Michaelis-Menten constant (K_m) and maximal velocity (V_max) of the enzyme for pectin were 88.54 μmol/ml and 175.44 μmol/mg/min, respectively. The enzyme activity was enhanced by Ca²⁺, Cu²⁺, and Na⁺, and strongly inhibited by Pb²⁺ and Mn²⁺. The pectin hydrolysates were mainly galacturonic acid and other oligo-galacturonates. Therefore, these characteristics suggest that the recombinant endo-PgaA may be of potential use in the food and feed industries.

• Proceedings of the KSRS Conference
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• v.1
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• pp.34-37
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• 2006

When tsunami waves propagate across open ocean they are steered by Coriolis force and refraction due to gentle gradients in the bathymetry on scales longer than the wavelength. When the wave encounters steep gradients at the edges of continental shelves and at the coast, the wave becomes non-linear and conservation of momentum produces squirts of surface current at the head of submerged canyons and in coastal bays. HF coastal ocean radar is well-conditioned to observe the current bursts at the edge of the continental shelf and give a warning of 40 minutes to 2 hours when the shelf is 50-200km wide. The period of tsunami waves is invariant over changes in bathymetry and is in the range 2-30 minutes. Wavelengths for tsunamis (in 500-3000 m depth) are in the range 8.5 to over 200 km and on a shelf where the depth is about 50 m (as in the Great Barrier Reef) the wavelengths are in the range 2.5 - 30 km. It is shown that the phased array HF ocean surface radar being deployed in the Great Barrier Reef (GBR) and operating in a routine way for mapping surface currents, can resolve surface current squirts from tsunamis in the wave period range 20-30 minutes and in the wavelength range greater than about 6 km. There is a trade-off between resolution of surface current speed and time resolution. If the radar is actively managed with automatic intervention during a tsunami alert period (triggered from the global seismic network) then it is estimated that the time resolution of the GBR radar may be reduced to about 2 minutes, which corresponds to a capability to detect tsunamis at the shelf edge in the period range 5-30 minutes. It is estimated that the lower limit of squirt velocity detection at the shelf edge would correspond to a tsunami with water elevation of less than 5 cm in the open ocean. This means that the GBR HF radar is well-conditioned for use as a monitor of small and medium scale tsunamis, and has the potential to contribute to the understanding of tsunami genesis research.

• Journal of Environmental Science International
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• v.19 no.11
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• pp.1423-1436
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• 2010

The effects of meteorological and reclaiming conditions on the reduction of suspended particles are investigated using a computational fluid dynamics (CFD) model with the k-$\varepsilon$ turbulence closure scheme based on the renormalization group (RNG) theory. Twelve numerical experiments with different meteorological and reclaiming conditions are performed. For identifying the meteorological characteristics of the target area and providing the inflow conditions of the CFD model, the observed data from the automatic weather station (AWS) near the target area is analyzed. Complicated flow patterns such as flow distortion, horse-shoe vortex, recirculation zone, and channeling flow appeared due to the topography and buildings in the domain. Specially, the flow characteristics around the reclamation area are affected by the reclaiming height, reclaiming size and windbreak height. Reclaiming height affected the wind speed above the reclaiming area. Windbreak induces more complicated flow patterns around the reclaiming area as well as within the reclaiming area. In front of the windbreak, flow is distorted as it impinges on the windbreak. As a result, upward flow is generated there. Behind the windbreak, a secondary circulation, so called, a recirculation zone is generated and flow is reattached at the end of the recirculation zone (reattachment point). At the lower part of the recirculation zone, there is a reverse flow toward the windbreak. Flow passing to the reattachment point starts to be recovered. Total amounts of suspended particles are calculated using the frictional and threshold frictional velocities, erosion potential function, and the number of surface disturbance. In the case of a 10 m-reclaiming and northerly wind, the amount of suspended particles is largest. In the presence of 5 m windbreak, the friction velocity above the reclaiming area is largely reduced. As a result, the total amount of the suspended particles largely decreases, compared to the case with the same reclaiming and meteorological conditions except for the windbreak The calculated suspended particle amounts are used as the emission rate of the dispersion model simulations and the dispersion characteristics of the suspended particles are analyzed.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.6 no.2
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• pp.67-75
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• 1986

This paper introduces the nonlinear Stream Function Wave Theory for design waves efficiently to compute the wave energy and energy transport quantities and to analyze the effects of nonlinearities on them. The Stream Function Wave Theory was developed by Dean for case of the observed waves with assymmetric wave profiles and of the design waves with symmetric theoretical wave profiles. Dalrymple later improved the computational procedure by adding two Lagrangian constraints so that more efficient convergence of the iterative numerical method to a specified wave height and to a zero mean free surface displacement resulted. And the Stream Function coefficients are computed numerically by the improved Marquardt algorithm developed for this study. As the result of this study the effects of nonlinearities on the wave quantities of the average potential energy density, the average kinetic energy density result in overestimation by linear wave theory compared to the Stream Function Wave Theory and increase monotonically with decreasing $L^*/L_O$ and with increasing $H/H_B$. The effects of nonlinearities on the group velocity and the wavelength quantities result in underestimation by linear wave theory and increase monotonically with increasing $H/H_B$. Finally the effect of nonlinearity on the average total energy flux results in overestimation for shallow water waves and underestimation for deep water waves by linear wave theory.

• Journal of the Korean Applied Science and Technology
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• v.30 no.2
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• pp.320-332
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• 2013

The feed air to MSW incinerator influences on the residence time of combustion gas, removal of unburnt ash and exiting gas temperature. Thus the secondary air volume could present sufficient residence time which can maintain the exiting temperature over $850^{\circ}C$. The secondary air also relates directly with the turbulence in the inside of combustion chamber, which finally provide the stable combustion condition. The present study designed a modern incinerator for a field scale, and evaluation of the potential amount of primary air based on the daily combustible quantity. From the evaluated primary air volume, the secondary air flow rate could be estimated, and its dynamic behavior was verified. In addition, the obtained air volume enables to find an optimum operation condition of the combustion. As a result of the CFD simulation, the air ratio 75 : 25 between primary and secondary air amount was optimum ratio than design criteria 72 : 28. And the flow velocity ratio of front-back of secondary air jet nozzle was found excellent at 1 : 3. In addition, the result of applied to the plant, the removal efficiency of NOx and CO generation would concentration of CO.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.1
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• pp.127-131
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• 2020

Objective: This study was conducted to determine catalytic properties of wheat phytase with exopolyphosphatase activity toward medium-chain and long-chain inorganic polyphosphate (polyP) substrates for comparative purpose. Methods: Exopolyphosphatase assay of wheat phytase toward polyP75 (medium-chain polyP with average 75 phosphate residues) and polyP1150 (long-chain polyP with average 1150 phosphate residues) was performed at pH 5.2 and pH 7.5. Its activity toward these substrates was investigated in the presence of Mg²⁺, Ni²⁺, Co²⁺, Mn²⁺, or ethylenediaminetetraacetic acid (EDTA). Michaelis constant (K_m) and maximum reaction velocity (V_max) were determined from Lineweaver-Burk plot with polyP75 or polyP1150. Monophosphate esterase activity toward p-nitrophenyl phosphate (pNPP) was assayed in the presence of polyP75 or polyP1150. Results: Wheat phytase dephosphorylated polyP75 and polyP1150 at pH 7.5 more effectively than that at pH 5.2. Its exopolyphosphatase activity toward polyP75 at pH 5.2 was 1.4-fold higher than that toward polyP1150 whereas its activity toward polyP75 at pH 7.5 was 1.4-fold lower than that toward polyP1150. Regarding enzyme kinetics, K_m for polyP75 was 1.4-fold lower than that for polyP1150 while V_max for polyP1150 was 2-fold higher than that for polyP75. The presence of Mg²⁺, Ni²⁺, Co²⁺, Mn²⁺, or EDTA (1 or 5 mM) exhibited no inhibitory effect on its activity toward polyP75. Its activity toward polyP1150 was inhibited by 1 mM of Ni²⁺ or Co²⁺ and 5 mM of Ni²⁺, Co²⁺, or Mg²⁺. Ni²⁺ inhibited its activity toward polyP1150 the most strongly among tested additives. Both polyP75 and polyP1150 inhibited the monophosphate esterase activity of wheat phytase toward pNPP in a dose-dependent manner. Conclusion: Wheat phytase with an unexpected exopolyphosphatase activity has potential as a therapeutic tool and a next-generational feed additive for controlling long-chain polyP-induced inappropriate inflammation from Campylobacter jejuni and Salmonella typhimurium infection in public health and animal husbandry.

• Journal of the Korea institute for structural maintenance and inspection
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• v.16 no.6
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• pp.155-162
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• 2012

When RC (Reinforced Concrete) structures are exposed to sea water, steel corrosion can occur and this leads a degradation of structural performance. Referring the electro-deposition system with sea water from the 1st step research, durability and structural performance are evaluated in coated steel and RC members containing it in the 2nd research. In the durability performance test, Half Cell Potential test is performed and the coated steel is evaluated to have the high resistance to corrosion, which shows only 35% of corrosion velocity in normal (bare) steel. In the structural performance test, tensile strength, adhesive strength, and flexural/shear in RC member are performed. For the electro-deposit coated steel, increasing ratios of 3.2% and 8.8% are evaluated in the test of tensile strength and adhesive strength, respectively. For the structural test in RC member, there is no big difference between RC members with coated and non-coated steel in ultimate load and failure pattern It is evaluated that the chemical compound with $CaCO_3$ and $Mg(OH)_2$ from electro-deposition causes slightly increased structural performance. The electro-deposit coated steel can be more widely applied after performance verification from several tests like fatigue, resistance to impact, and long term-submerging test.

• Journal of the Korean Association of Geographic Information Studies
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• v.11 no.2
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• pp.38-50
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• 2008

The site-specific seismic responses and corresponding seismic hazards are influenced mainly by the subsurface geologic and geotechnical dynamic characteristics. To estimate reliably the seismic responses in this study, a geotechnical information system (GTIS) within GIS framework was developed by introducing new concepts, which consist of the extended area containing the study area and the additional site visit for acquiring surface geo-knowledge data. The GIS-based GTIS was built for Gyeongju area, which has records of abundant historical seismic hazards reflecting the high potential of future earthquakes. At the study area, Gyeongju, intensive site investigations and pre-existing geotechnical data collections were performed and the site visits were additionally carried out for assessing geotechnical characteristics and shear wave velocity ($V_S$) representing dynamic property. Within the GTIS for Gyeongju area, the spatially distributed geotechnical layers and $V_S$ in the entire study area were reliably predicted from the site investigation data using the geostatistical kriging method. Based on the spatial geotechnical layers and $V_S$ predicted within the GTIS, a seismic zoning map on site period ($T_G$) from which the site-specific seismic responses according to the site effects can be estimated was created across the study area of Gyeongju. The spatial $T_G$ map at Gyeongju indicated seismic vulnerability of two- to five-storied buildings. In this study, the seismic zonation based on $T_G$ within the GIS-based GTIS was presented as regional efficient strategy for seismic hazard prediction and mitigation.

• Korean Journal of Applied Biomechanics
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• v.17 no.4
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• pp.99-106
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• 2007

The gait instability in the elderly has been associated with age-related deterioration in physical strength and reducing the potential for elderly falls requires regular exercise. In 2005, National Center for Injury Prevention and Control(NCIPC) reported that most elderly falls occur during activities in daily living(ADL). To better reveal biomechanic mechanisms underlying age-related degeneration in gait stability, and to enhance the assessment of falls risk, an accurate quantification of a person's balance maintenance during locomotion is needed. Instantaneous orientation of the line connecting COP and COM can characterize whole body position with respect to the supporting foot during gait and the angle between this line and the vertical line passing through the COP known as a good assessment to detect the elderly gait instability. Therefore the purpose of this study was to investigate a 6-month walking exercise effects in reducing elderly fall risk factors by using COP-COM inclination angles. Twenty-two community-dwelling elderly participated this study. The participants performed a walking exercise(3 times/week, 1 hour/visit) for 6 months. Laboratory kinematics during walking was assessed at months 0, 3 and 6. Significant increased in gait velocity was found among periods(p=.011, $1.25{\pm}.03$, $1.32{\pm}.03$, and $1.39{\pm}.04\;m/s$ in 0-, 3-, and 6-month, respectively). Also, significant differences in anterior and posteriror inclination angles were found among the periods(p<.05; posterior inclination angles: $12.8{\pm}2.2$, $11.0{\pm}2.9$, & $10.9{\pm}1.9$; anterior inclination angles: $13.7{\pm}1.7$, $14.6{\pm}3.2$, & $1.46{\pm}.21$ in 0month, 3month, & 6month, respectively). These findings provide evidence of significant reduced fall risk factors of community-living older adults associated with a systematic walking program.