• Mycobiology
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• 제49권3호
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• pp.258-266
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• 2021

The velvet regulators VosA and VelB are primarily involved in spore maturation and dormancy. Previous studies found that the VosA-VelB hetero-complex coordinates certain target genes that are related to fungal differentiation and conidial maturation in Aspergillus nidulans. Here, we characterized the VosA/VelB-inhibited developmental gene vidD in A. nidulans. Phenotypic analyses demonstrated that the vidD deleted mutant exhibited defect fungal growth, a reduced number of conidia, and delayed formation of sexual fruiting bodies. The deletion of vidD decreased the amount of conidial trehalose, increased the sensitivity against heat stress, and reduced the conidial viability. Moreover, the absence of vidD resulted in increased production of sterigmatocystin. Together, these results show that VidD is required for proper fungal growth, development, and sterigmatocystin production in A. nidulans.

• BMB Reports
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• 제47권5호
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• pp.274-279
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• 2014

Bortezomib has been known as the most promising anti-cancer drug for multiple myeloma (MM). However, recent studies reported that not all MM patients respond to bortezomib. To overcome such a stumbling-block, studies are needed to clarify the mechanisms of bortezomib resistance. In this study, we established a bortezomib-resistant cell line (U266/velR), and explored its biological characteristics. The U266/velR showed reduced sensitivity to bortezomib, and also showed cross-resistance to the chemically unrelated drug thalidomide. U266/velR cells had a higher proportion of CD138 negative subpopulation, known as stem-like feature, compared to parental U266 cells. U266/velR showed relatively less inhibitory effect of prosurvival NF-${\kappa}B$ signaling by bortezomib. Further analysis of RNA microarray identified genes related to ubiquitination that were differentially regulated in U266/velR. Moreover, the expression level of CD52 in U266 cells was associated with bortezomib response. Our findings provide the basis for developing therapeutic strategies in bortezomib-resistant relapsed and refractory MM patients.

• The Plant Pathology Journal
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• 제31권2호
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• pp.108-114
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• 2015

Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China, and several key pathogenic factors have been isolated. An yeast two-hybrid (Y2H) library is a very useful platform to further unravel novel pathogenic factors in C. lunata. To construct a high-quality full length-expression cDNA library from the C. lunata for application to pathogenesis-related protein-protein interaction screening, total RNA was extracted. The SMART (Switching Mechanism At 5' end of the RNA Transcript) technique was used for cDNA synthesis. Double-stranded cDNA was ligated into the pGADT7-Rec vector with Herring Testes Carrier DNA using homologous recombination method. The ligation mixture was transformed into competent yeast AH109 cells to construct the primary cDNA library. Eventually, a high qualitative library was successfully established according to an evaluation on quality. The transformation efficiency was about $6.39{\times}10^5$ transformants/$3{\mu}g$ pGADT7-Rec. The titer of the primary cDNA library was $2.5{\times}10^8cfu/mL$. The numbers for the cDNA library was $2.46{\times}10^5$. Randomly picked clones show that the recombination rate was 88.24%. Gel electrophoresis results indicated that the fragments ranged from 0.4 kb to 3.0 kb. Melanin synthesis protein Brn1 (1,3,8-hydroxynaphthalene reductase) was used as a "bait" to test the sufficiency of the Y2H library. As a result, a cDNA clone encoding VelB protein that was known to be involved in the regulation of diverse cellular processes, including control of secondary metabolism containing melanin and toxin production in many filamentous fungi was identified. Further study on the exact role of the VelB gene is underway.

• 천문학회지
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• 제32권2호
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• pp.109-117
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• 1999

UBVI CCD photometry has been obtained for a region around the Wolf-Rayet star WR 12. We found two young stellar associations in the observed field: the nearer one comprises the field members of Vela OBI association at d = 1.8kpc, while the farther one is the young open cluster Bochum 7 (Bo 7) at d = 4.8kpc. The stars associated with Bo 7 showed no central concentration which suggests that Bo 7 is not a young open cluster but simply a local concentration in the density of young stars belonging to the OB association (Vel OB3). These two associations have similar ages but remarkably different mass function slopes ($\Gamma$ = -2.1 $\pm$ 0.3 for Vel OBI and -1.0 $\pm$ 0.3 for Bo 7). The stars in Vel OBI shows an evident age spread (${\Delta}T\~ 9Myr$). We also found two strong H$\alpha$ emission stars - WR 12 and $\sharp$1066 - from narrow band H$\alpha$ photometry.

• Journal of Electrical Engineering and Technology
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• 제11권2호
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• pp.499-507
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• 2016

Medical imaging techniques such as Magnetic Resonance Imaging (MRI), Computed Tomography (CT) and Ultrasound (US) produce a large amount of digital medical images. Hence, compression of digital images becomes essential and is very much desired in medical applications to solve both storage and transmission problems. But at the same time, an efficient image compression scheme that reduces the size of medical images without sacrificing diagnostic information is required. This paper proposes a novel threshold-based medical image compression algorithm to reduce the size of the medical image without degradation in the diagnostic information. This algorithm discusses a novel type of thresholding to maximize Compression Ratio (CR) without sacrificing diagnostic information. The compression algorithm is designed to get image with high optimum compression efficiency and also with high fidelity, especially for Peak Signal to Noise Ratio (PSNR) greater than or equal to 36 dB. This value of PSNR is chosen because it has been suggested by previous researchers that medical images, if have PSNR from 30 dB to 50 dB, will retain diagnostic information. The compression algorithm utilizes one-level wavelet decomposition with threshold-based coefficient selection.

• 한국자원식물학회지
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• 제4권2호
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• pp.39-45
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• 1991

Proximate compositions of the roots of cultured and wild Codonopsis lanceolata were examined as tile basic reasearch for the study of their source of processed foods.No significant difference in the contents was found between the wild root and thecultivated at 3$0^{\circ}C$ in biotronroom. In view of the results to have measured mineralswhich is included in 14 sorts of Codonopsis lanceolata and surveyed their distribution.12 kinds of minerals including T1, Co, Ge, Sm, Mo, Sc, Be, Cd, As, Ga, Bi, ph are ne-vel or little included in almost source. Other twenty-one sorts of minerals (Ni, Se,Ba, Sb, Si, Ti, B, Li, Ifs, Ca, Sr, Mn, Fe, Cu, Zn, p, Al, Na, V, Cr, K) are more or less in-cluded in all source and Ca, Mg, p, K, and Fe are metals that are included in large qu-entities in comparison with others. No minerals difference in the contents was foundbetween the cultivated temperature. The content of elements of inorganic metal differsaccording to the part of C. lanceolata.

• Nuclear Engineering and Technology
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• 제52권3호
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• pp.589-596
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• 2020

Many components in the assembly section of Sodium-cooled Fast Reactor are made of good corrosionresistant 316 LN Stainless Steel material. To avoid self-welding of the components with the coolant sodium at elevated temperature, hardfacing is inevitable. Ni-based colmonoy-5 is used for hardfacing due to its lower dose rate by Plasma Transferred Arc process due to its low dilution. Since Ni-Cr-B-Si alloy becomes very fluidic while depositing, the major height of the weld overlay rests inside the groove. Hardfacing is also done over the plain surface where grooving is not possible. Therefore, grooved and ungrooved hardfaced specimens were prepared at different travel speeds. Fe content at every 100 ㎛ of the weld overlay was studied by Energy Dispersive Spectroscopy and also the micro hardness was determined at those locations. A correlation between iron dilution from the base metal and the micro hardness was established. Therefore, if the Fe content of the weld overlay is known, the hardness at that location can be obtained using the correlation and vice-versa. A new correlation between micro hardness and dilution coefficient is obtained at different locations. A comparative study between those specimens is carried out to recommend the optimum travel speed for lower dilution.

• Bulletin of the Korean Chemical Society
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• 제33권11호
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• pp.3755-3760
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• 2012

Single crystals of lithium potassium phthalate (LiKP) were successfully grown from aqueous solution by solvent evaporation technique. The grown crystals were characterized by single crystal X-ray diffraction. The lithium potassium phthalate $C_{16}\;H_{12}\;K\;Li_3\;O_{11}$ belongs to triclinic system with the following unit-cell dimensions at 298(2) K;$a=7.405(5){\AA}$;$b=9.878(5){\AA}$;$c=13.396(5){\AA}$;${\alpha}=71.778(5)^{\circ}$;${\beta}=87.300(5)^{\circ}$;${\gamma}=85.405(5)^{\circ}$; having a space group P1. Mass spectrometric analysis provides the molecular weight of the compound and possible ways of fragmentations occurs in the compound. Thermal stability of the crystal was also studied by both simultaneous TGA/DTA analyses. The UV-Vis-NIR spectrum shows a good transparency in the whole of Visible and as well as in the near IR range. Third order nonlinear optical studies have also been studied by Z-scan technique. Nonlinear absorption and nonlinear refractive index were found out and the third order bulk susceptibility of compound was also estimated. The results have been discussed in detail.

• 한국미생물·생명공학회지
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• 제44권4호
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• pp.409-419
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• 2016

사상성 진균인 Aspergillus spp.은 환경 속에서 흔히 찾을 수 있는 진균으로 인류에 유익한 역할을 주기도 하지만 병원성을 일으키기도 한다. 대부분의 Aspergillus spp.는 무성 포자를 생성하여 번식을 하며 다양한 이차 대사 산물을 합성한다. 여러 연구에 따르면 Velvet 조절자들은 진균 특이적 전사 인자로 모델 진균인 Aspergillus nidulans의 성장, 분화 및 이차 대사산물 생성 등 다양한 부분에서 중요한 역할을 한다고 보고되었다. 또한 Velvet 단백질들은 다양한 복합체를 형성하며, 이 복합체들은 Aspergillus nidulans에서 다양한 역할을 한다. 다른 Aspergillus spp.에서 Velvet 단백질들은 매우 유사한 구조를 가지며 진균의 무성 생식과 이차 대사를 조절한다. 이번 논문에는 Aspergillus spp.의 Velvet 단백질들의 기능에 대하여 요약하였다.

• Current Photovoltaic Research
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• 제7권4호
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• pp.103-110
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• 2019

Cu(In,Ga)₃Se₅ (β-CIGS) has a band gap of 1.35 eV, which is an optimum value for high solar-energy conversion efficiency. The effects of Cu and Ga content on the cell performance were investigated previously. However, the effect of Se content on the cell performance is not well understood yet. In this work, β-CIGS films were fabricated by three-stage co-evaporation of elemental sources with various Se fluxes at the third stage instead of at all stages. The average composition of five samples was Cu_1.05(In_0.59,Ga_0.41)3Se_y, where the stoichiometric y value is 5.03 and the stoichiometric Se/metal (Se/M) ratio is 1.24. We varied the Se/metal ratio in a range from 1.18 to 1.28. We found that the best efficiency was achieved when the Se/M ratio was 1.24, which is exactly the stoichiometric value where the CIGS grains on the CIGS surface were tightly connected and faceted. With the optimum Se/M ratio, we were able to enhance the cell efficiency of a β-CIGS solar cell from 9.6% to 12.0% by employing a Na₂S post deposition treatment. Our results indicate that Na₂S post deposition treatment is very effective to enhance the cell efficiency to a level on par with that in α-CIGS cell.