• Biomolecules & Therapeutics
• /
• v.12 no.4
• /
• pp.221-227
• /
• 2004

Nonsteroidal anti-inflammatory drugs (NSAIDs), particularly the highly selective cyclooxygenase (COX)-2 inhibitors have been shown to decrease the growth of tumor, in part, by inhibition of neovascularization. Recently, besides mature endothelial cells, endothelial progenitor cells (EPCs) have been shown to contribute neovascularization in angiogenic tissues. In this study, we addressed a question whether nimesulide, a selective COX-2 inhibitor, could affect differentiation of EPCs into adhesive endothelial cells in vitro. Total mononuclear cells were isolated from cord blood by Ficoll density gradient centrifugation, and then the cells were incubated with nimesulide or vehicle control for 7 days. The number of adherent and spindle-shaped cells decreased by nimesulide treatment in a concentration-dependent fashion at a concentration range of 5 - 200 ${\mu}M$. Moreover, the adherent cells double positive for DiI-ac-LDL uptake and lectin binding significantly decreased upon nimesulide treatment. There was no change of expression of CD31 between treatment and control groups, whereas slight reduction was detected upon treatment in expression of VE-cadherin, ICAM-1, vWF, ${\alpha}v$, and ${\alpha}5$. Nimesulide also reduced cell viability during first 3 days' culture and induced apoptosis in adherent EPCs, resulting in increased annexin-V-positive and propidium iodide-negative cells. Taken together, these results suggest that nimesulide could be applied for the inhibition of new vessel formation, in part, by inhibiting differentiation and survival of EPCs.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.37 no.2
• /
• pp.397-407
• /
• 2017

This study investigated current status of rest area for drowsy drivers on the highways and drew the related issues to define specifications and design criteria regarding expressway rest area for drowsy drivers on the highways. Based on the investigation result, geometric structure specifications and improvement plans are suggested. The entry part of a rest area for drowsy drivers on the highways was divided into deceleration transition section, deceleration lane and entry connection road while the exit part was divided into exit connection road, acceleration lane and acceleration transition section. The optimum length was estimated by considering the main lane vehicle traveling speed, traveling speed at the beginning/end point of entry/exit connection roads, deceleration and acceleration. In addition, reasonable design criteria were suggested by dividing the parking section of rest area for drowsy drivers according to parking style and cross-section composition, and length of parking space and then considering the ratio of vehicles using rest area for drowsy drivers, the ratio of heavy vehicles, and the design speed within a rest area for drowsy drivers. It is believed that the suggested design criteria on rest area for drowsy drivers on the highways can be utilized in the future planning and maintenance of rest area for drowsy drivers. Additionally, the defined criteria on installing rest area for drowsy drivers on the highways will prevent traffic accidents in resting facilities and highways as well as improve usage and safety of them.

• Korean Journal of Environmental Agriculture
• /
• v.18 no.2
• /
• pp.135-139
• /
• 1999

A new effective and economic method was developed, removing interferences such as chlorophyll and lipid from leaves with small amounts of reagents and solvents in order to analyse PAHs(Polycyclic Aromatic Hydrocarbons). The extract from a soxhlet containing $4{\sim}5g$ of leaves and 100ml of dichlormethane and refluxed for 20 hrs was concentrated and eluted with 60ml of a hexane:dichloromethane (1:1) mixture through a column of 9mm wide inner diameter and 130mm long, packed from the bottom with 2.5g of $Al_2O_3$, 1.5g of $SiO_2$and 2g of anhydrous $Na_2SO_4$. The eluent was concentrated and loaded on a GPC column of 20mm wide inner diameter and 280mm long, packed with 12g of Bio-beads. The column was washed with 37ml of the hexane:dichloromethane(1:1) mixture. Another 43ml of the mixture was eluted as a PAH fraction and collected. This eluent was concentrated under gentle nitrogen to $50{\mu}l$ and analysed using GC-MS. The recoveries, obtained by comparing with the amounts of the internal standards of deuterated PAHs were $43.3{\sim}107.5%$(RSD $2.2{\sim}9.5%$).

• Korean Journal of Food Science and Technology
• /
• v.49 no.2
• /
• pp.192-202
• /
• 2017

In this study, factors involved in lipid and energy metabolism following treatment with ethanolic extract of the Polygonatum sibiricum rhizome (ID1216) were evaluated in high-fat diet-induced obese mice. ID1216-treated mice showed a significant reduction in weight gain compared to non-treated mice. ID1216 treatment increased the protein levels of AMP-dependent protein kinase, sirtuin1, peroxisome proliferator-activated receptor ${\gamma}$ coactivator 1-${\alpha}$ ($PGC1{\alpha}$), peroxisome proliferator-activated receptor ${\alpha}$ ($PPAR{\alpha}$) and uncoupling proteins in the adipose tissue, liver and muscle compared to vehicle treatment. Analysis of downstream signals of the sirtuin1 $PGC1{\alpha}$-$PPAR{\alpha}$ pathway showed that ID1216 regulates the expression of ${\beta}$-oxidation related genes such as acyl-CoA oxidase, carnitine palmitoyltransferase1, acyl-CoA dehydrogenase and adipocyte protein 2. In addition, ID1216 increased the expression of adipose triglyceride lipase. These results suggest that ID1216 has anti-obesity effects by regulating the genes involved thermogenesis, ${\beta}$-oxidation and lipolysis in a diet-induced obesity model.

• Applied Chemistry for Engineering
• /
• v.28 no.4
• /
• pp.490-494
• /
• 2017

With increasing the number of vehicles, the accident rate also goes up and the damaged vehicles should be painted as a final repair process. At the painting stage the solvent-based paint causes environmental problems. To overcome these problems waterborne refinish paint is frequently used recently. However, for waterborne refinish, the costs of insurance coverage are too expensive, and insurance reimbursement costs could be burdensome. Because of the high price of aquatic paint treatment, the service shop might charge the malicious service price. In this study, the surfactant of Surfynol 104, which is the component in the paint, was used as an indicator whether the vehicle was painted with waterborne paint. The specimen was quantitatively analyzed to contain 0.38% of the surfactant through the standard addition method with isotope substituted internal standard (IS) of fluranthene-d10 by curie point pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS).

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.41 no.3
• /
• pp.226-232
• /
• 2013

A compact hydrogen generation device of fuel cell system using chemical hydride storage technique was designed to fit the propulsion device requirement of a small unmanned aerial vehicle(SUAV). For high efficient, compact, and lightweight hydrogen generation control device, the Co-B catalyst hydrogen conversion rate by $NaBH_4$ aqueous solution flux is measured so that the proper amount of Co-B catalyst for maximum hydrogen generation of 100W stack was proposed. A compact hydrogen generation device is controlled by pump's on/off using its own internal pressure and consumes fuel in high efficiency through a dead-end type fuel cell. The fuel cell system has stable operation for a planed flight profile. The system operates up to maximum 7 hours and at least 4 hours for tough flight profiles.

• Journal of Korean Society of Transportation
• /
• v.24 no.3 s.89
• /
• pp.103-112
• /
• 2006

For recent innovation of If technology and the beginning of Digital Multimedia Broadcasting (DMB) service, it has been dramatically increased to setup TV system in a car for watching TV and receiving traffic Information. Watching TV while driving would distract a driver s cognitive and visual attention as eating food, operating the radio, using a cell phone. However, there is paucity of empirical researches and it is uncertain how watching TV in driving impacts on the driver's cognition in the concrete. Therefore, we surveyed domestic drivers on the attitude watching TV while driving as well as conducted experiments through a driving simulator. Especially, we recruited two groups of participants to explore the effects of watching TV on driving behavior. The result proved that the participants who watched TV while their driving had relatively narrower the attention span than the Participants who did not watch TV. Also, those who watched TV drove with less stability and more urgent operations of the brake and accelerator than those who did not watched TV Finally, we discussed limitations and implications of the study.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.18 no.11
• /
• pp.1217-1223
• /
• 2007

Advanced microwave absorbers for wide oblique incidence angles are required in many applications including wireless communication or vehicle identification in ITS(Intelligent Transport System) where 5.8 GHz DSRC(Dedicated Short Range Communication) system is applied. In this study, two-layered microwave absorber(with a laminate structure of dielectric/magnetic composites) has been designed for the achievement of low reflection coefficient over wide incidence angles at 5.8 GHz. Iron flake particles are used as the filler in the absorbing layer, and the magnetic composite sheet exhibits high magnetic loss due to ferromagnetic resonance in gigahertz frequencies. The surface layer of low dielectric constant containing small amount of carbon black is used as the impedance transformer. On the basis of transmission line theory, the reflection loss has been calculated for the two-layer structure with variation of incident angles for both TE(Transverse Electric) and TM(Transverse Magnetic) polarizations. At the optimum thickness of the composite layers, a low value of reflection loss(less than -10 dB) has been predicted for wide incidence angles up to $55^{\circ}$ which is in good agreement with the measured value determined by free-space measurement.

• Journal of Korean Society of Transportation
• /
• v.28 no.3
• /
• pp.169-183
• /
• 2010

Image-based traffic information collection systems have entered widespread adoption and use in many countries since these systems are not only capable of replacing existing loop-based detectors which have limitations in management and administration, but are also capable of providing and managing a wide variety of traffic related information. In addition, these systems are expanding rapidly in terms of purpose and scope of use. Currently, the utilization of image processing technology in the field of traffic accident management is limited to installing surveillance cameras on locations where traffic accidents are expected to occur and digitalizing of recorded data. Accurately recording the sequence of situations around a traffic accident in a freeway and then objectively and clearly analyzing how such accident occurred is more urgent and important than anything else in resolving a traffic accident. Therefore, in this research, existing technologies, this freeway attribute, velocity changes, volume changes, occupancy changes reflect judge the primary. Furthermore, We pointed out by many past researches while presenting and implementing an active and environmentally adaptive methodology capable of effectively reducing false detection situations which frequently occur even with the Gaussian Mixture model analytical method which has been considered the best among well-known environmental obstacle reduction methods. Therefore, in this way, the accident was the final decision. Also, environmental factors occur frequently, and with the index finger situations, effectively reducing that can actively and environmentally adaptive techniques through accident final judgment. This implementation of the evaluate performance of the experiment road of 12 incidents in simulated and the jang-hang IC's real-time accident experiment. As a result, the do well detection 93.33%, false alarm 6.7% as showed high reliability.

• International Journal of Highway Engineering
• /
• v.8 no.1 s.27
• /
• pp.99-106
• /
• 2006

During the dry periods, many types of pollutant are accumulating on the paved surface by vehicle activities. Particularly, the highways are stormwater intensive landuses because of high imperviousness and high pollutant mass emissions from vehicles. The accumulated pollutants in highways are washed-off during a rainfall event and are highly contributing on water quality of receiving water bodies. The stormwater runoff from the highways are containing various pollutants such as metals, oil & grease and toxic chemicals originated from vehicles. Therefore, this research is performed to find pollutant characteristics in the magnitude of statistical pollutant concentrations during storm periods. During the monitoring periods, the first-flush phenomenon is visibly occurred on most storm events, which is confirmed from hydro- and pollute-graphs. The 95% confidence intervals of washed-off pollutant concentration are ranged to 154.7-257.1 mg/L for 755,138.9-197.6 mg/L for COD, 3.5-6.4 mg/L for oil & grease, 6.3-9.2 mg/L for TN and 2.3-3.31 mg/L for TP. The first flush effect is mostly occurred within initial 30 min of storm duration.