• Journal of Plant Biology
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• 제5권2호
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• pp.1-5
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• 1962

Attempts were made in order to make clear the meiotic irregularities, male gametophyte formation and spermatogenesis for the purpose of applying these embryological facts to taxonomic works. Followings are the results obtained : 1. Meiosis is extremely irregular, giving rise to giant, micro, and empty, pollens. Meiotic division in each PMC is observed synchronizing. 2. In the microspore immediately before the vegetative-generative nuclear division, the nucleus locates appressed to the inner wall, most of the other part of the cell being occupied by large vacuoles. 3. Spindle symmetry in the microspore nuclear division is of intermediate type. 4. Nuclear divisions in each micropore are not synchronized as in meiosis. 5. Generative nucleus is located in close contact with the inner wall, while the vegetative nucleus occupies the central part of pollen grain. The pollen in this stage looks somewhat like broad bean. Afterward the generative nucleus loses contact with the wall, the nuclear shape changing from lenticular to roundsh. 6. The generative nucleus in fully matured pollen grain usually takes the shape of crescence, those in abortive pollen, lenticular or ellipsoidal, etc.

• Journal of Plant Biology
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• 제36권3호
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• pp.241-249
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• 1993

The pattern of RNA synthesis during male gametogenesis of Brassica napus was studied using 3H-uridine autoradiography. No incorporation of isotope occurred in the newly released microspore and the nonvacuolate, furrowed microspore. Peak incorporation of label during male gametogenesis occurred in the uninucleate, furrowed microspores showing various degrees of vacuolation. In this microspore stage, silver grains were localized in the nucleus and cytoplasm. Moderate incorporation of the isotope occurred in the nulceus of the vacuolated microspore. After the microspore mitosis, isotope incorporation occurred predominantly in the nucleus of the vegetative cell with little or no incorporation in the generative cell. In tricellular pollen, no incorporation of isotope was observed in both the vegetative nucleus and the sperms. Silver grains almost completely disappeared from tricellular mature pollen grains ready to germinate.

• Current Research on Agriculture and Life Sciences
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• 제31권2호
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• pp.75-82
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• 2013

Haploids are plants with a gametophytic number of chromosomes in their sporophytes. Androgenesis occurs from asymmetric division of pollen grains into generative cells and vegetative cells, followed by re-entry of the vegetative cell during S-phase, which causes microspores progress into G2/M transition in culture. One of the most interesting features of haploids is the possibility to produce doubled haploid (DH) individuals. Doubled haploidy is extremely useful to plant breeders because it enables shortened breeding periods and efficiency in selection of useful recessive agronomic traits. Doubled-haploid technology is not only applicable to breeding, but also to transformation programs of desired genes. In addition to practical breeding programs, DH lines provide useful materials of fundamental genetics including exploitation of QTLs and genes conferred with various agronomic traits by establishing DH populations. This paper provides historical overviews on androgenesis and describes several mechanisms associated with pollen embryogenesis, including mode of actions in pollen embryogenesis, mechanisms of chromosome doubling and factors affecting androgenesis. We also discuss recent progress in application of haploids to breeding, genes associated with in vitro response and drawbacks to anther culture for application of doubled haploids in crop breeding.

• 식물조직배양학회지
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• 제26권3호
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• pp.149-156
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• 1999

도라지 약배양에 있어서 저온처리가 화분 2형현상과 배형성에 미치는 영향을 조사하기 위해 1핵성 소포자기의 도라지 약을 0.5 mg/L NAA와 1.0mg/L BA가 첨가된 MS배지에 배양하였다. 저온전처리는 이상화분과 균등분열에 의한 B형 화분, 다핵 및 다세포 화분수를 현저하게 증가시켰는데 특히 8$^{\circ}C$에서 5일간 저온전처리는 배양 전 이상화분이 20.6%가 증가되었고 배양 중에는 54.9%의 캘러스와 9.9%의 배형성율을 나타내 가장 효과적이었다. 배양 중 소포자로부터 배는 첫째. 주로 영양세포가 분열하거나 둘째, 영양세포와 생식세포가 각각 분열하거나 셋째, 균등분열에 의한 영양핵과 동일한 크기의 두 낭세포 모두가 분열하여 배를 형성하는 3가지 경로가 관찰되었다.

• Journal of Plant Biotechnology
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• 제2권3호
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• pp.129-134
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• 2000

Clear visualization of pepper (Capsicum annuum L.) microspore nuclei with common stains such as acetocarmine or propionocarmine is difficult, hindering cytological analysis. The DAPI stain after the addition of ferric chloride solution to fixative resulted in clear visualization of nuclei. For clear visualization of nuclei and slight fluorescence of microspore wall, addition of 40-60 ${mu}ell$ of ferric chloride solution to the 1 $m\ell$ fixative was identified as most effective. At all stages of gametophytic development, the nuclei can be distinctly visualized. Starch granules does not intefere with the fluorochrome, and so the vegetative and generative nuclei were cleary visible in binucleate pollens. With its rapidity and reliability, this technique represents an efficient tool for routine staging or investigation of the nuclear status of the microspore during culture.

• 원예과학기술지
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• 제29권1호
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• pp.36-42
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• 2011

토마토 펄라이트 자루재배에서 급액방법으로 배액전극제어법 적용시 일회급액량이 작물의 생육과 수확량에 미치는 영향을 알아보고, 생육단계별 적정 일회급액량을 구명함으로써 적정 급액관리방법을 도출하기 위해 연구가 수행되었다. 처리로는 일회급액량을 적게 주는 처리(S-S)와 많이 주는 처리(L-L), 1화방수확기를 기점으로 적게 주다가 두 배로 급액량을 늘이는 처리(S-L)와 많이 주다가 반으로 줄이는 처리(L-S)를 두었다. 작물의 전 생육기간동안 일회급액량이 많거나 적은 처리에서는 배액율이 낮고 불안정해지며 생육이 좋지 않았다. 생육단계에 맞게 정식 후 1단 수확기 이전까지는 일회급액량을 약 145mL로 많이 주다가 이후에 70mL까지 줄여주는 L-S처리에서 뿌리분포가 좋고, 수확량이 가장 많았고, 배액율도 안정적이었다. 따라서 토마토 재배시 일회급액량을 생육단계에 따라 달리 해 주어 급액패턴을 다량소회에서 소량다회로 변화시켜 주는 것이 토마토의 생육과 수확량에 효과적임을 알 수 있었다. 또한, L-S처리로 일회급액량에 변화를 주었을 때, 토마토의 생장양상이 영양생장에서 생식생장으로 바뀌는 것을 식물생육지표 조사에서 확인하였다.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1158-1158
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• 2001

Fast and dynamic biochemical, enzymatic and morphological changes occur during the so-called generative development and during the vegetative processes in seeds. The most characteristic biochemical and compositional changes of this period are the formation and decline of storage components or their precursors, the change of their degree in polymerization and an extensive change in water content. The aim of the present study was to detect the maturation processes in seed nondestructively and to verify the applicability of near infrared spectroscopic methods in the measurement of physiological, chemical and biochemical changes in wheat seed. The amount and variation of different water “species” has been changed intensively during maturation. Characteristic changes of three water absorption bands (1920, 1420 and 1150 nm) during maturation were analysed. It was concluded that the free/bound transition of water molecules could be followed sensitively in different region of NIR spectra. Kinetic changes of carbohydrate reserves were characteristic during maturation. An intensive formation and decline of carbohydrate reserves were observed during early stage of maturation (0 -13 days, high energy demand). An accelerated formation of storage carbohydrates (starch) was detected in the second phase of maturation. Five characteristic absorption bands were analysed which were sensitive indicators the changes of carbohydrates occurred during maturation. Precursors of protein synthesis and the synthesis of reserve proteins and their kinetic changes during maturation were followed from NIR spectra qualitative and qualitatively. Dynamic formation of amino acids and the changes of N forms were detected by spectroscopic, chromatographic and by capillary electrophoresis methods. Calibration equations were developed and validated in order to measure the optimal maturation time protein and moisture content of developing wheat seeds. The spectroscopic methods are offering chance and measurement potential in order to detect fine details of physiological processes. The spectra have many hidden details, which can help to understand the biochemical background of processes.

• 농업과학연구
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• 제46권4호
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• pp.769-779
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• 2019

Chilli peppers are predominantly cultivated in open field systems under abiotic and biotic stress conditions. Abiotic and biotic factors have a considerable effect on plant performance, fruit quantity, and quality. Chilli peppers grow well in a tropical climate due to their adaptation to warm and humid regions with temperatures ranging from 18 to 30℃. Nowadays, chilli peppers are cultivated all around the world under different climatic conditions, and their production is gradually expanding. Expected climate changes will likely cause huge and complex ecological consequences; high temperature, heavy rainfall, and drought have adverse effects on the vegetative and generative development of all agricultural crops including chilli peppers. To gain better insight into the effect of climate change, the growth, photosynthetic traits, morphological and physiological characteristics, yield, and fruit parameters of chilli peppers need to be studied under simulated climate change conditions. Moreover, it is important to develop alternative agrotechnologies to maintain the sustainability of pepper production. There are many conceivable ideas and concepts to sustain crop production under the extreme conditions of future climate change scenarios. Therefore, this review provides an overview of the adverse impacts of climate change and discusses how to find the best solutions to obtain a stable chilli pepper yield.

• 대한약침학회지
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• 제22권1호
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• pp.16-27
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• 2019

Generative and vegetative parts of the cactuses have had a long-lasting position in folk medicine and their effects could partly be confirmed in scientific experiments. Nowadays, the cactus, fruits, and cladodes are the focus of many studies because of their desirable properties. Therefore, the summarized reports of valuable properties of medicinal plants may be a good way to familiarize researches with a new source of drugs with lower side effects and higher efficacy. Opuntia dillenii, a well-known member of the Cactaceae family, is used as a medicinal plant in various countries and grows in the desert, semi-desert, tropical and sub-tropical areas. It shows diverse pharmacological activities such as: antioxidant, anti-inflammatory, anti-tumor, neuroprotective, hepatoprotective, hypotensive etc. OD fruit also possesses valuable constitutes for instance: betalains, ascorbic acid, total phenol, protein as well as essential elements which suggest the significant potential of this plant as a complementary therapy against several pathological conditions. This review describes experimental evidence about pharmacological and therapeutic potential of OD in order to give the basis of its application in the prevention and treatment of some chronic diseases. More studies on OD can help better understanding of its pharmacological mechanism of action to explain its traditional uses and to identify its potential new therapeutic applications.

• 한국작물학회지
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• 제26권2호
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• pp.185-191
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• 1981

옥수수 생장발육의 한 Scheme을 작성하기 위하여 조생종 MIC-1과 만생종 수원 19호를 공시하여 1980년 4월 25일부터 6월 24일까지 12일 간격으로 6파종기를 두고, 휴폭 60cm에 주간 15cm로 파종하여 본시험을 실시하였다. 여기에서는 작물체의 주요기관 즉 엽, 기, 자종의 형태적 변화를 주로 조사하였으며 그 결과를 요약하면 다음과 같다. 1. 엽신의 발육을 그 전개정도에 따라 10등액으로 구분하여 Leaf Development Index(LDI)를 정의하였다. 2. 엽출현율(LAR)은 평균 3일로써 품종간, 재배시기간 큰 차가 없었다. 3. 출현 후 약 1개월 후에 절간이 신장하기 시작하여 절위별로 신장하며 지상부 제 1절이 벽출되는 시기는 제 8-9엽기에 해당되었다. 4. Silks의 형태적 변화는 종실의 발달과 밀접한 관계가 있었다(표 4참조). 5. 영양생장기간은 품종간 및 재배시기간에 차이가 현저하였지만 생식생장기간은 별차이가 없었다. 6. 완전전개된 엽수, 비대된 절수 그리고 Silks의 형태적 변화 및 종실발달 등으로 옥수수의 Scheme을 제시하였다.