• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.30 no.6_2
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• pp.623-629
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• 2012

During the construction of crossing engineering one of the important measures to ensure the safety of subway operation is the implementation of deformation surveying to the existing subway tunnel. Guangzhou new subway line 2 engineering which crosses the existing tunnel is taken as the background. How to achieve intelligent and automatic deformation surveying forecast during the subway tunnel construction process is studied. Because large amount of surveying data exists in the subway construction, deformation analysis is difficult and prediction has low accuracy, a subway intelligent deformation prediction model based on the PBIL and support vector machine is proposed. The PBIL algorithm is used to optimize the exact key parameters combination of support vector machine though probability analysis and thereby the predictive ability of the model deformation is greatly improved. Through applications on the Guangzhou subway across deformation surveying deformation engineering the prediction method's predictive ability has high accuracy and the method has high practicality. It can support effective solution to the implementation of the comprehensive and accurate surveying and early warning under subway operation conditions with the environmental interference and complex deformation.

• Journal of Institute of Control, Robotics and Systems
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• v.20 no.6
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• pp.635-640
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• 2014

Detection and diagnosis of incipient bearing fault in an induction motor is important for the prevention of serious motor failure. This paper presents an analysis of the effect of a faulty bearing on the stator current of an induction motor. A bearing fault leads to torque oscillations which result in phase modulation of the stator current. Since the torque oscillations cause specific frequency components at the stator current spectrum to rise sharply, the bearing fault can be detected by checking out the faultrelated frequency. In this paper, a mathematical model of the load torque oscillation caused by a bearing fault is presented. The proposed model can be used to analyze the physical phenomenon of a bearing fault in an induction motor. In order to represent the bearing fault effect, the proposed model is combined with an existing model of vector-controlled induction motors. A set of simulation results demonstrate the effectiveness of the proposed model and represent that bearing fault detection using a stator current is useful for vector-controlled induction motors.

• Animal cells and systems
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• v.16 no.6
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• pp.455-461
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• 2012

Amongst the various antimicrobial peptides, lysozyme plays a central role in initiating and maintaining the antibacterial defense response of insect. Here we propose the biosynthesis and refolding of recombinant lysozyme in Escherichia coli expressed in inclusion body form. The Agrius lysozyme gene was amplified using gene specific primers and then ligated into the pGEX-4T-1 vector, which contained the glutathione S-transferase (GST) gene as a fusion partner. A recombinant lysozyme was expressed in E. coli Rosetta cells using a pGEX-4T-1 expression vector, and the fusion protein was induced by ioporpyl-${\beta}$-D-thiogalactopyranoside (IPTG). The recombinant protein produced as an inclusion body was resolubilized in solubilization buffer, and the resultant solution was dialyzed in refolding buffer. After thrombin cleavage, the recombinant lysozyme was purified by ion exchange chromatography and reverse phase chromatography. The recombinant lysozyme was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis and immunoreactivity against the anti-Agrius lysozyme was observed by western blot analysis of this protein. The recombinant lysozyme displayed antibacterial activity against Bacillus megaterium and Micrococcus luteus, which was confirmed by the inhibition zone assay.

• Animal cells and systems
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• v.12 no.3
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• pp.149-155
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• 2008

We have isolated and characterized Agrius convolvuli cDNA encoding a c-type lysozyme. The cDNA sequence encodes a processed protein of 139 amino acid residues with 19 amino acid residues amino-terminal signal sequence and 120 amino acid residues mature sequence. The amino acid residues responsible for the catalytic activity and the binding of the substrate are conserved. Agrius lysozyme has a high identity to Manduca sexta. Recombinant A. convolvuli lysozyme was expressed in Escherichia coli BL21(DE3) pLysS cells for pGEX 4T-1 expression vector. Their optimal conditions for the fusion protein expression and purification were screened. Lysozyme gene amplified with primers ACLyz BamHI and ACLyz XhoI was ligated into the pGEX 4T-1 vector, which contained the glutathione S-transferase(GST) gene for fusion partner. The fusion protein was induced by IPTG and identified by SDS-PAGE analysis. Molecular weight of the fusion protein was estimated to be about 45 kDa. Recombinant lysozyme, fused to GST, was purified by glutathion-Sepharose 4B affinity chromatography. Western blot analysis of this protein revealed an immunoreactivity with the anti-Agrius lysozyme.

• Journal of Digital Contents Society
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• v.5 no.4
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• pp.289-294
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• 2004

For the purpose of image analysis, we usually take the application method relying on the various mathematical theories. On the respect of image as two variable function one may uses the gradient vector or several type of energy functions induced by the conventional (partial) derivative. We also have used the tangent plane or curvature vector from the concept of differential geometry {**]. However, these mathematical tools my assume that the given function should be sufficiently smoothing enough to depict every local variation continuously. But the real application of these mathematical methods to the natural images or phenomena may occur the ill-posed problem. In this paper, we have defined the weak derivative as a loose form of the derivative so that it my applied to the irregular case with less ill-posed problem.

• Ocean and Polar Research
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• v.40 no.1
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• pp.49-58
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• 2018

This study aimed to analyze the causality of hairtail prices among import and domestic distribution channels using a Vector Error Correction Model(VECM). The results are as follows. First, since the ADF unit-root test suggests that each of the price variables, apart from retail price, has a unit root, the price variables should be 1st-differenced to secure the stability of the prices. Next, through the Johansen co-integration test, it was discovered that there are long-term relationships among the price variables. On the basis of the co-integration test, VECM analysis shows that the producer price has a long-run balance with the import and wholesale prices. In particular, when the prices deviate from the balance, the producer price dynamically adjusts to return to the long-term relationship among prices. It also indicates that the producer price has an impact on the import, wholesale, and retail prices in the short-term, and the import price has an influence on the producer and wholesale prices. In addition, the impulse response analysis demonstrates that the impulse of import and producer prices has a lasting impact on each of the prices.

• Magazine of the Korean Society of Agricultural Engineers
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• v.36 no.1
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• pp.83-94
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• 1994

The first part of this study dealt with the determination of soil parameters for Lade's double work-hardening model using the raw data obtained from cubical and cylinderal triaxial tests At present, it should be investigated which test can simulated satisfactorily the behavior of soft clayey foundation. In this regard, plate bearing test on the 2-dimentional model foundation(218cm long, 40cm wide, 19&m high) was performed, and finite element analysis carried out to abtain the behavior of the foundation. Settlement, lateral displacement, displacement vector and mode of failure were measured and these values were compared with numerical values in order to validate the numerical program developed by authors. The FEM technique was based on Christain-Boehmer's method, in which the displacement is obtained at each nodal point while stress and pore water pressure at each element.In this research, Biot's equation, which explains was elahorately the phisical meaning of consolidation, was selected, as a governing equation, coupled with Lade's double surface work-hardening constitutive model.

• Proceedings of the KOSOMBE Conference
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• v.1995 no.05
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• pp.227-229
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• 1995

This paper attempts to examine an optimal method for the chromosome specific vector extraction. Usually, represented method are used with a line segmentation on a chromosome Image. It is not Inaccurate but also needs a long time for the analysis. This paper purpose to aquire specific vector in the image with a using optimal ellipse estimation method. Normally, shapes of chromosomes are curved and too difficult to analyze automatically. A chromosome has a lot of band which looks like an ellipse. If we can estimate their bands with an ellipse, we can reconstruct the sample Which Is straight and can be analyzed easily. We have rearranged a chromosome Image with above proposed. Result shows a reconstructed sample which Is simple for chromosome analysis.

• Journal of Power Electronics
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• v.16 no.3
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• pp.1097-1109
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• 2016

Fault detection and isolation are related to system monitoring, identifying when a fault has occurred, and determining the type of fault and its location. Fault detection is utilized to determine whether a problem has occurred within a certain channel or area of operation. Fault detection and diagnosis have become increasingly important for many technical processes in the development of safe and efficient advanced systems for supervision. This paper presents an integrated technique for fault diagnosis and classification for open- and short-circuit faults in three-phase inverter circuits. Discrete wavelet transform and principal component analysis are utilized to detect the discontinuity in currents caused by a fault. The features of fault diagnosis are then extracted. A fault dictionary is used to acquire details about transistor faults and the corresponding fault identification. Fault classification is performed with a fuzzy logic system and relevance vector machine (RVM). The proposed model is incorporated with a set of optimization techniques, namely, evolutionary particle swarm optimization (EPSO) and cuckoo search optimization (CSO), to improve fault detection. The combination of optimization techniques with classification techniques is analyzed. Experimental results confirm that the combination of CSO with RVM yields better results than the combinations of CSO with fuzzy logic system, EPSO with RVM, and EPSO with fuzzy logic system.

• Journal of Korea Port Economic Association
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• v.21 no.1
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• pp.133-151
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• 2005

The purpose of this paper is to investigate the international competition power between Korean ports and Chinese ports according to the port efficiency scores of DEA(Data Envelopment Analysis) by newly introducing the priority vector of AHP(Analytic Hierarchy Process) to the DEA method. Empirical analysis shows the followings: First, there was not big changes of DEA rankings when we use the input-oriented CCR and BCC models after introducing the AHP priority vectors to the input variables. Yantian Port's competition power was declined, but that of Busan Port was up in the BCC model. Second, there was some changes of DEA rankings when we use the output-oriented CCR and BCC models after introducing the AHP priority vectors to the output variables. Rankings of Dalian, Qingdao, Shanghai Ports were up. But Shekou, Yantian Ports showed the declined ranking position in the CCR model. In the BBC model, rankings of Shanghai and Busan Ports were up. But those of Shekou and Yantian Ports were declined. The main policy implication based on the findings of this study is that The Ministry of Maritime Affairs & Fisheries in Korea and China should introduce AHP and DEA approaches when they measure the international competition power by using the porrt efficiency scores of DEA.