• The Journal of the Acoustical Society of Korea
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• v.33 no.4
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• pp.248-254
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• 2014

Among acoustic signal analysis tasks, acoustic context awareness is one of the most formidable tasks in terms of complexity since it requires sophisticated understanding of individual acoustic events. In conventional context awareness methods, individual acoustic event detection or recognition is employed to generate a relevant decision on the impending context. However this approach may produce poorly performing decision results in practical situations due to the possibility of events occurring simultaneously or the acoustically similar events that are difficult to distinguish with each other. Particularly, the babble noise acoustic event occurring at a bus or subway environment may create confusion to context awareness task since babbling is similar in any environment. Therefore in this paper, a frequency-cepstral feature vector is proposed to mitigate the confusion problem during the situation awareness task of binary decisions: bus or metro. By employing the Support Vector Machine (SVM) as the classifier, the proposed feature vector scheme is shown to produce better performance than the conventional scheme.

• Development and Reproduction
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• v.7 no.1
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• pp.35-40
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• 2003

Gonadotropin releasing hormone(GnRH), which is secreted from the hypothalamus, has a pivotal role in the reproduction of mammals. Golden hamsters are seasonal breeding mammal and their sexual activity is determined by photoperiod(length of light per day). Long photoperiod(LP, $\leq$ 12.5 hours of light) maintains the reproductive activity and short photoperiod(SP, $\leq$ 12 hours of light) suppresses it. In order to investigate in detail, the sexual activity was individually examined in SP-housed male golden hamsters received a vector at three different concentrations which contains rat GnRH cDNA. The gonadal regression was significantly(P<0.05) accelerated by the highest concentrations of the vector at 8 and 10 weeks after the treatment in comparison to the other groups. In the light of pulsatile release of GnRH in maintaining reproductive activity, the vector containing GnRH cDNA might secrete the GnRH in a constant high level. These results suggest that the GnRH-containing vector might desensitize the anterior pituitary, leading to acceleration or testicular regression.

• Journal of the Korean Institute of Intelligent Systems
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• v.18 no.5
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• pp.718-723
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• 2008

Bearing is one of the important mechanical elements used in various industrial equipments. Most of failures occurred during the equipment operation result from bearing defects and breakages. Therefore, monitoring of bearings is essential in preventing equipment breakdowns and reducing unexpected loss. The purpose of this paper is to present an online monitoring method to predict bearing states using vibration signals. Bearing vibrations, which are collected as a form of profile signal, are first analyzed by a discrete wavelet transform. Next, some statistical features are obtained from the resultant wavelet coefficients. In order to select significant ones among them, analysis of variance (ANOVA) is employed in this paper. Statistical features screened in this way are used as input variables to support vector machine (SVM). An hierarchical SVM tree is proposed for dealing with multi-class problems. The result of numerical experiments shows that the proposed SVM tree has a competent performance for classifying bearing fault states.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.43 no.7
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• pp.601-608
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• 2015

The static aeroelastic analysis and optimization of flexible wings are conducted for steady state conditions while both aerodynamic and structural parameters can be used as optimization variables. The system of multidisciplinary design optimization as a robust methodology to couple commercial codes for a static aeroelastic optimization purpose to yield a convenient adaptation to engineering applications is developed. Aspect ratio, taper ratio, sweepback angle are chosen as optimization variables and the skin thickness of the wing. The real-coded adaptive range multi-objective genetic algorithm code, which represents the global multi-objective optimization algorithm, was used to control the optimization process. The support vector regression(SVR) is applied for optimization, in order to reduce the time of computation. For this multi-objective design optimization problem, numerical results show that several useful Pareto optimal designs exist for the flexible wing.

• Journal of Korea Multimedia Society
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• v.8 no.7
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• pp.889-897
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• 2005

This paper presents a new fast motion estimation algorithm for multi-reference frames. We first analyze the experimental results of the successive elimination algorithm, which is a fast version of full search algorithm, being applied to Multi-reference frames. Based on the analysis, a new scheme for alleviating its computational burden is introduced. In the proposed method, the motion vector for the immediately previous reference frame is found by applying the successive elimination algorithm, while the motion vector for other reference frames is estimated by extrapolation of the already obtained motion vector. Adaptively restricting the motion search area to the local area centered on the estimated motion vector, the proposed method provides dramatic computational complexity reduction but slight quality degradation. The proposed method is evaluated by experiments for some image sequences.

• Journal of Sericultural and Entomological Science
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• v.40 no.1
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• pp.52-59
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• 1998

For the construction of plasmid and bmNPV sarrying the FRT recognition site for the FLP recombinases, we synthesized the wild type FRT dligonucleotides. The target FRT sequences consist of three 13bp repeated DNA sequences; two repeats in a direct orientation and one inverted relative to the other two. In addition, there is an 8bp spacer region between the repeats which determune the orientation of the FRT recombination site. In order to place the FRT site both in target BmNPV genome and the transfer vector, we constructed a plasmid, FRT site both in the target BmNPv genome and the transfer vector, we constructed a plasmid, pFRT$\beta$-gal, carrying the FRT sites within the cloning sites of pSV vector and a recombinant BmNPV, vFRTPH, carrying the FRT sites at a downstream of polyhedrin promotor, respectively. In order to test the functionality of the FLP/FRT site-specific recombination system, vFRTPH, pFRT$\beta$-gal and pHsFLP DNA were co-transfected into BmN-4 cells. The resulting recombinant virus was designated a vFRT$\beta$2-gal. From construction analysis of the vFRT$\beta$2-gal with PCR technique it was concluded that the entire pFRT$\beta$-gal plasmid with $\beta$-galactosidase gene and origines of replication flanked by two functional hybrid FRT sequences. The efficiency of recombination was 8.7%, which was higher than that(2.2%) of recombination between a conventional transfer vector and the wild type BmNPV.

• BMB Reports
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• v.38 no.1
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• pp.41-48
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• 2005

In this study we developed the transposon-mediated shuttle vector 'Hanpvid', which composed of HaNPV (Heliothis armigera nuclear polyhedrosis virus) genomic DNA and a transposon cassette from Bacmid of Bac-to-Bac system. Hanpvid replicates in E. coli in the same way as Bacmid and retains infective function in cotton bollworm cells (Hz-AM1). Using Hanpvid we constructed a recombinant virus, which could infect Hz-AM1 cells and generate recombinant HaNPV (rHa-Bar) containing the barnase gene, a ribonuclease gene from Bacillus amyloliquefaciens. Since the expression vector carrying barnase gene cannot replicate in the absence of barstar, a specific inhibitor of barnase, we constructed a new cotton bollworm cell line (AM1-NB) using the marker rescue method. In AM1-NB barstar was integrated into the cellular chromosome to sustain the replication of rHa-Bar. To screen out recombinant HaNPV for potential use as biopesticide, Hz-AM1 and AM1-NB cell lines were infected with rHa-Bar, respectively. The results obtained indicate that Viral progenies in AM1-NB were 23 and 160 times greater than those in Hz-AM1 48 h and 72 h after infection, respectively. With additional insertion of the polyhedron gene from AcNPV (Autographa californica nuclear polyhedrosis virus) into the Hanpvid genome, rHa-Bar regained the polyhedron phenotype and its pest-killing rate greatly improved. Toxic analysis showed that the lethal dosages ($LD_{50}$) and the lethal time(s) ($LT_{50}$) of rHa-Bar were reduced by 20% and 30%, respectively, compared to wt-HaNPV in the third instar larvae of cotton bollworm. This study shows that in AM1-NB barnase can be effectively produced and used as pest-killing agent for the biological control of cotton pests.

• Journal of Microbiology and Biotechnology
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• v.19 no.3
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• pp.307-313
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• 2009

In order to systemically investigate the possibility of using coxsackievirus B3 (CVB3) to deliver foreign genes in vivo, a recombinant strain of CVB3 encoding the renilla gene (CVB3-renilla) was constructed. The recombinant CVB3 resulted in extensive and transient expression of the renilla protein within mouse organs, especially the pancreas. The level of expression was generally dependent upon the viral titer present. Moreover, the CVB3-renilla strain was completely attenuated. Interestingly, the recombinant CVB3 vector was expressed much more strongly in mouse organs than was a comparable adenoviral vector. The CVB3-renilla strain did not express the renilla gene in mice with pre-existing coxsackievirus-specific neutralizing antibodies, but direct organ-specific administration of the virus during open-peritoneum surgery was able to circumvent this immunity. This coxsackievirus vector may represent a useful means for delivering and expressing foreign genes in mouse models in an acute and extensive fashion.

• Journal of Microbiology and Biotechnology
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• v.17 no.7
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• pp.1213-1216
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• 2007

The vector pCW5 with plasmid pC7, originally isolated in Lactobacillus paraplantarum C7 derived from kimchi, was constructed using a p32 strong promoter, the pC7 replicon, and green fluorescent protein (GFP) as the reporter. The constructed vector was transformed into E. coli and Leuconostoc mesenteroides, and GFP expression detected using a Western blot analysis. GFP fluorescence was recognized in E. coli and Leuconostoc mesenteroides using a confocal microscope. In addition, GFP fluorescence was also clearly detected in several industrially important lactic acid bacteria (LAB), including Lactobacillus bulgaricus, Lactobacillus paraplantarum, and Lactobacillus plantarum. Thus, pCW5 was shown to be effective for Leuconostoc mesenteroides when using GFP as the reporter, and it can also be used as a broad-host-range vector for other lactic acid bacteria.

• The Korean Journal of Applied Statistics
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• v.18 no.2
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• pp.253-269
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• 2005

One of the basic assumptions of the regression models is that the parameter vector does not vary across sample observations. If the parameter vector is not constant for all observations in the sample, the statistical model is changed and the usual least squares estimators do not yield unbiased, consistent and efficient estimates. This study investigates the regression model with some or all parameters vary across partitions of the whole sample data when the model permits different response coefficients during unusual time periods. Since the usual test for overall homogeneity of regressions across partitions of the sample data does not explicitly identify the break points between the partitions, the testing the equality between subsets of coefficients in two or more linear regressions is generalized and combined with the test procedure to search the break point. The method is applied to find the possibility and the turning point of the structural change in the long-run unemployment rate in the usual static framework by using the regression model. The relationships between the variables included in the model are reexamined in the dynamic framework by using Vector Autoregression.