• Journal of Microbiology and Biotechnology
• /
• 제11권1호
• /
• pp.85-91
• /
• 2001

To construct an efficient Bacillus subtilis expression vector, strong promoters were isolated from the chromosomal DNA libraries of Clostridium acetobutylicum ATCC 4259, Thermoactinomyces sp. E79, and Bacillus thermoglucosidasius KCTC 3400. The $P_{C27}$ promoter cloned from the clostridial chromosmal DNA showed a 5-fold higher promoter strength than the $P_{SP02}$ promoter in the expression of the cat gene, and its sequence was estimated as an upstream region of the predicted hypothetical gene (tet-R family bacterial transcription regulator gene) in C. acetobutylicum. As a promoter element, $P_{C27}$ exhibited putative nucleotide sequences that can bind with bacterial RNAP and the 3'end of the 16S rRNA just upstream of the start codon. In addition, the promoter activity of $P_{C27}$ was distinctively repressed in the presence of glucose. Using $P_{C27}$ as the promoter element, a glucose controllable B. subtilis expression vector was constructed and the lipase gene from Staphylococcus haemolyticus KCTC 8957P was expressed in B. subtilis. When compared with the lipase expression by the T7 promoter induced by IPTG in E. coli, the $P_{C27}$ promoter showed about a 1.5-fold higher expression level in B. subtilis than that without induction.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2003년도 생물공학의 동향(XII)
• /
• pp.539-542
• /
• 2003

SDS-PAGE 결과 elution pH에 따라 분리되는 band pattern은 비슷하게 2band의 양상을 보이지만, FI값을 비교하여 보았을 때 다른 pH보다 8.0에서 가장 높은 수준을 보였으므로 GFPuv/cytochrome c-552 fusion Protein의 분리 ${\cdot}$ 정제에 가장 적합한 pH를 8.0으로 정하였다.

• 한국연초학회지
• /
• 제13권2호
• /
• pp.34-41
• /
• 1991

To explore the possibility of introducing Zea mays transposable element Ac(activator) which can be used as a mutagen and gene tag in tobacco plants other than maitre, we tried to introduce a cloned Ac element into tobacco cells by an Agrobacterium tumefaciens binary vector system. Transformation of N. babacum cv. Burley 21 tissues and regeneration to whole plant were carried out. The frequency of the transformed callus induced in shoot induction media was higher than that of transformed callus induced in callus induction media. However, the calli were not grown in the second selection media, and became yellow senescent calli. Regenerated tobacco plantlets with foreign gene were also obtained in shoot induction media containing 100 $\mu\textrm{g}$/ml kanamycin and 100$\mu\textrm{g}$/ml carbenicillin. The leaf tissues of transformant was also resistant to 1000 $\mu\textrm{g}$/ml kanamycin. The chromosomal DNAs of transformant and normal plant of N. tabacum were digested by EcoR I and Hind III but not by Pst I.

• 한국안전학회지
• /
• 제30권3호
• /
• pp.93-99
• /
• 2015

Economic burden of work-related musculoskeletal disorder(WMDs) is increasing. Known causes of WMDs include improper posture, repetition, load, and temperature of workplace. Among them, improper postures play an important role. A smart sensor called SensorTag is employed to estimate the trunk postures including flexion-extension, lateral bend, and the trunk rotational speeds. Measuring gravitational acceleration vector in the smart sensor along the tri-orthogonal axes offers an orientation of the object with the smart sensor attached to. The smart sensor is light in weight and has small form factor, making it an ideal wearable sensor for body posture measurement. Measured data from the smart senor is wirelessly transferred for analysis to a smartphone which has enough computing power, data storage and internet-connectivity, removing need for additional hardware for data post-processing. Based on the estimated body postures, WMDs risks can be conviently gauged by using existing WMDs risk assesment methods such as OWAS, RULA, REBA, etc.

• 한국미생물·생명공학회지
• /
• 제23권6호
• /
• pp.784-788
• /
• 1995

The lambda Integrase (Int) carries out site-specific recombination between the two partner DNA sequences, attachment P (attP) and attachment B (attB). In order to study the recombination mechanism, a large quantity of pure integrase is required. Then, we constructed an int gene inserted recombinant plasmid (pNYL3) by using the pQE31 HIS-Tag vector, and produced the fusion protein, 6xHIS-Int from the E. coli TG1 strain carrying the pNYL3 plasmid. The recombinant protein produced was purified by phosphocellulose and Ni$^{++}$-NTA affinity column chromatographies. The result of the in vitro recombination assay using the standard reaction mixture containing 6xHIS-Int and partially purified integration host factor (IHF) showed that the 6xHIS-Int tagged recombination Integrase had the full recombination activity.

• 한국정보과학회:학술대회논문집
• /
• 한국정보과학회 2006년도 한국컴퓨터종합학술대회 논문집 Vol.33 No.1 (A)
• /
• pp.79-81
• /
• 2006

커널(Kernel)을 이용한 분류 방법은 넓은 마진(large margin) 분류기로서 SVM(Support Vector Machine)을 주로 사용하게 된다 하지만, 이 방법은 라그랑제 파라미터(Lagrange Parameter)의 최적화 과정을 포함함으로써 학습 과정을 쉽지 않게 만든다. 이 최적화 과정은 특히 DNA computing과 같은 단순한 과정의 설계를 통해 결과를 얻어야 하는 새로운 계산 모델에 커널을 적용하고자 했을 경우 큰 장벽이 된다. 본 논문에서는 넓은 마진을 목표로 하는 최적화 과정이 아닌 다른 라벨(label)의 데이터간의 경계 파악을 위한 간단한 커널 갱신 방법의 도입을 통해 분류기를 설계한다. 이 방법을 가우시안 커널에 적용시켜 본 결과, 반복을 통해 데이터의 구조를 찾아갈 수 있는 특성을 보여주며, 결국 넓은 마진의 최적화된 파라미터를 찾게 됨을 보여준다. 본 논문에서는 이 최적화 방법을 DNA 분자를 이용한 커널 생성 모델인 DNA 커널에 적용시켰을 때 잘 알려진 AML/ALL 데이터를 잘 분류해 냄을 보여준다.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제2권2호
• /
• pp.86-89
• /
• 1997

Epidermal growth factor(EGF) known as a urgastrone is a powerful mitogen with a wide variety of possibilities for medical usages. A mature EGF coding region was isolated from human prepro-EGF sequence by a conventional PCR and cloned into pQE vector in which the gene product was supposed to be expressed with 6$\times$His tag for the subsequent purification. The recombinant mature EGF was expressed in M15[Rep4], an Escherichia coli host strain, in amount of 30-40% of total proteins pressent in E. coli extract by the addition of isopropylthio-$\beta$-galactopyranoside (IPTG). The recombinant EGF purified using a Ni2+-NTA affinity colume chromatography was active in its ability to induce phosphorylation on tyrosine residues of several substrate proteins when murine NH3T3 and human MRC-5 fibroblast cells were stimulated with it. This work may provide the basic technology and information for the production of recombinant EGF.

• 한국정보과학회:학술대회논문집
• /
• 한국정보과학회 2005년도 가을 학술발표논문집 Vol.32 No.2 (2)
• /
• pp.259-261
• /
• 2005

분류 방법으로서의 SVM(Support Vector Machine)은 커널 방법과 함께 사용됨으로써 그 유용성을 크게 향상시켰다. 커널 방법은 일반적으로 입력 데이터의 자질(feature)로 나타내는 공간으로부터 높은 차원의 공간으로 데이터를 사상(mapping)시키는 역할을 하게 되나, 기본적으로는 데이터간에 새로운 거리(metric)를 부설해주는 역할을 하는 것이다. 지금까지 나온 다양한 커널 방법은 구조화된(structured) 데이터에 대해 커널 형태로 거리를 부여하는 방법을 제시한다. 본 논문에서는 DNA의 작용을 모델링하여 만든 새로운 커널이 miRNA(micro RNA)와 mRNA(messenger RNA)쌍에 대한 발현 여부를 분류해 내기 위해 커널 형식으로 거리를 부여하는 방법을 보인다. 이 방법은 실리콘 컴퓨터가 아닌 실제 DNA분자로 실험할 수 있도록 설계된 것을 고려할 때 여러 종류의 DNA 코드를 분석하는 데 사용될 수 있는 새로운 분자컴퓨팅 방법이다.

• International Journal of Computer Science & Network Security
• /
• 제24권4호
• /
• pp.113-118
• /
• 2024

Pursuance Sentiment Analysis on Twitter is difficult then performance it's used for great review. The present be for the reason to the tweet is extremely small with mostly contain slang, emoticon, and hash tag with other tweet words. A feature extraction stands every technique concerning structure and aspect point beginning particular tweets. The subdivision in a aspect vector is an integer that has a commitment on ascribing a supposition class to a tweet. The cycle of feature extraction is to eradicate the exact quality to get better the accurateness of the classifications models. In this manuscript we proposed Term Frequency-Inverse Document Frequency (TF-IDF) method is to secure Principal Component Analysis (PCA) with Naïve Bayes Classifiers. As the classifications process, the work proposed can produce different aspects from wildly valued feature commencing a Twitter dataset.

• 미생물학회지
• /
• 제52권4호
• /
• pp.495-499
• /
• 2016

본 연구에서는 발광 세균에 존재하는 LuxG 단백질의 효소학적 성질을 알아내기 위하여 Photobacterium leiognathi ATCC 25521의 luxG 유전자를 중합효소연쇄반응으로 증폭시켜 T5 프로모터와 6X His-tag 시스템을 지닌 pQE 벡터에 삽입시킨 재조합플라스미드를 제조하여 대장균에 형질전환 후 과발현시켜 단백질을 분리, 정제 하였다. 정제된 단백질의 효소학적 실험 결과, 이 단백질은 FMN과 NADPH 기질에 대한 ferric iron reductase의 기능을 갖고 있음을 확인하였으며 이들 기질에 대한 효소 활성도 상수 $K_m$ 및 $V_{max}$ 값을 결정하였다.