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http://dx.doi.org/10.7845/kjm.2016.6059

Ferric iron reductase activity of LuxG from Photobacterium leiognathi  

Lee, Eui Ho (Department of Biochemistry, Chungnam National University)
Nam, Ki Seok (Department of Biochemistry, Chungnam National University)
Lee, Seon Kwang (Department of Biochemistry, Chungnam National University)
Oh, Eugeney (Department of Biochemistry, Chungnam National University)
Lee, Chan Yong (Department of Biochemistry, Chungnam National University)
Publication Information
Korean Journal of Microbiology / v.52, no.4, 2016 , pp. 495-499 More about this Journal
Abstract
In order to identify the biochemical characteristics of LuxG, the luxG gene from bioluminescence bacteria of Photobacterium leiognathi ATCC 25521 was isolated by PCR-Amplification and inserted into pQE30 vector containing the T5 promoter and 6X His-tag system. The resulting recombinant plasmid was transformed into Escherichia coli to over-express the luxG gene and purify the gene product. The purified LuxG protein demonstrated ferric iron reductase activity and the kinetic parameters of $K_m$ and $V_{max}$ for FMN as well as the NADPH substrates of ferric iron reductase were determined, respectively.
Keywords
bioluminescence; ferric iron reductase; flavin; LuxG; Photobacterium;
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