• Proceedings of the KSRS Conference
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• 1999.11a
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• pp.485-490
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• 1999

Wind vector information over the ocean is currently obtained using multiple beam scatterometer data. The scatterometers on ERS-1/2 generate wind vector information with a spatial resolution of 50km and accuracies of $\pm$2m/s in wind speed and $\pm$20$^{\circ}$ in wind direction. Synthetic aperture radar (SAR) data over the ocean have the potential of providing wind vector information independent of weather conditions with finer resolution. Finer resolution wind vector information can often be useful particularly in coastal regions where the scatterometer wind information is often corrupted because of the lower resolution system characteristics which is often contaminated by the signal returns from the coastal areas or ice in the case of arctic environments. In this paper we tested CMOD_4 and CMOD_IFR2 algorithms for extracting the wind vector from SAR data. These algorithms require precise estimation of normalized radar cross-section and wind direction from the SAR data and the local incidence angle. The CMOD series algorithms were developed for the C-band, VV-Polarized SAR data, typically for the ERS SAR data. Since RADARSAT operates at the same C-band but with HH-Polarization, the CMOD series algorithms should not be used directly. As a preliminary approach of resolving with this problem, we applied the polarization ratio between the HH and VV polarizations in the wind vectors estimation. Two test areas, one in front of Inchon and several sites around Jeju island were selected and investigated for wind vector estimation. The new results were compared with the wind vectors obtained from CMOD algorithms. The wind vector results agree well with the observed wind speed data. However the estimation of wind direction agree with the observed wind direction only when the wind speed is greater than approximately 3.0m/s.

• YAKHAK HOEJI
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• v.49 no.3
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• pp.217-224
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• 2005

Human Immunodeficiency Virus type 1 (HIV-l) based lentivirus vector has demonstrated great potential as gene therapy vectors mediating efficient gene delivery and long-term transgene expression in both dividing and nondividing cells. However, for clinical studies it must be confirmed that vector preparations are safe and not contaminated by replication competent lentivirus (RCL) related to the parental pathogenic virus, HIV-l. In this study, we would like to establish the method for titration and RCL detection of lentivirus vector. The titration was determined by vector expression containing the green fluorescent protein, GFP in transduced cells. The titer was $1{\times}10^7$ Transducing Unit/ml in the GFP expression assay and $8.9{\times}10^7$ molecules/ml in the real-time PCR. Also, for the detection of RCL, we have used a combination method of PCR and p24 antigen detection. First, PBS/psi and VSV-G region in the genomic DNA of transduced cells was detected by PCR assay. Second, transfer and expression of the HIV-1 gag gene was detected by p24 ELISA. In an attempt to amplify any RCL, the transduced cells were cultured for 3 weeks (amplification phase) and the supernatant of amplified transduced cell was used for the second transduction to determine whether a true RCL was present (indicator phase). Analysis of cells and supernatant at day 6 in indicator phase were negative for PBS/psi, VSV-G, and p24 antigen. These results suggest that they are not mobilized and therefore there are no RCL in amplification phase. Thus, real-time PCR is a reliable and sensitive method for titration and RCL detection of lentivirus vector.

• Journal of Microbiology and Biotechnology
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• v.34 no.4
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• pp.804-811
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• 2024

Foamy viruses (FVs) are generally recognized as non-pathogenic, often causing asymptomatic or mild symptoms in infections. Leveraging these unique characteristics, FV vectors hold significant promise for applications in gene therapy. This study introduces a novel platform technology using a pseudo-virus with single-round infectivity. In contrast to previous vector approaches, we developed a technique employing only two vectors, pcHFV lacking Env and pCMV-Env, to introduce the desired genes into target cells. Our investigation demonstrated the efficacy of the prototype foamy virus (PFV) dual-vector system in producing viruses and delivering transgenes into host cells. To optimize viral production, we incorporated the codon-optimized Env (optEnv) gene in pCMV-Env and the Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE) at the 3' end of the transgene in the transfer vector. Consequently, the use of optEnv led to a significant enhancement in transgene expression in host cells. Additionally, the WPRE exhibited an enhancing effect. Furthermore, the introduced EGFP transgene was present in host cells for a month. In an effort to expand transgene capacity, we further streamlined the viral vector, anticipating the delivery of approximately 4.3 kbp of genes through our PFV dual-vector system. This study underscores the potential of PFVs as an alternative to lentiviruses or other retroviruses in the realm of gene therapy.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.10 no.1
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• pp.9-15
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• 2017

In this study we performed the experiment to detect the ERP using SVM with wavelet features. The EEG signal that is generated visual stimulated ERP database in SCCN applied for the experiment. The feature vectors for experiment are categorized frequency and continuous wavelet- based vectors. In experimental results, the detection rate of SVM with wavelet feature vectors improved above 10% comparing with frequency- based feature vector. Based on the experimental results we analyzed the relation between the activity degree of the ERP and the band split characteristics of the ERP by wavelet transform.

• Reproductive and Developmental Biology
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• v.40 no.1
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• pp.7-13
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• 2016

Effectiveness of transgene transfer into genome is crucially concerned in mass production of the bio-pharmaceuticals using genetically modified transgenic animals as a bioreactor. Recently, the mammary gland has been considered as a potential bioreactor for the mass production of the bio-pharmaceuticals, which appears to be capable of appropriate post-translational modifications of recombinant proteins. The mammary gland tissue specific vector system may be helpful in solving serious physiological disturbance problems which have been a major obstacle in successful production of transgenic animals. In this study, to minimize physiological disturbance caused by constitutive over-expression of the exogenous gene, we constructed new retrovirus vector system designed for mammary gland-specific expression of the hEPO gene. Using piggyBac vector system, we designed to express hEPO gene under the control of mammary gland tissue specific and lactogenic hormonal inducible goat ${\beta}$-casein or mouse Whey Acidic Protein (mWAP) promoter. Inducible expression of the hEPO gene was confirmed using RT-PCR and ELISA in the mouse mammary gland cells treated with lactogenic hormone. We expect the vector system may optimize production efficiency of transgenic animal and reduce the risk of global expression of transgene.

• Proceedings of the IEEK Conference
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• 2002.07b
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• pp.999-1002
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• 2002

In this paper, we proposes an adaptive method for reducing the computational overhead of fine-to-coarse MRME at the finest resolution level by considering for the spatial and spectral characteristics between wavelet decomposition levels simultaneously. As we know, there is high correlation between the adjacent blocks and it can give the very important clue to estimate motion at finest level. So, in this paper, using the initial motion vector and the adjacent motion vector in the coarsest level, we determine the optimal direction that will be minimized the estimation error in the finest level. In that direction, we define the potential searching region within the full searching region that is caused to increase much computational overhead in the FtC method. Last, in that region, we process the efficient 2-step motion estimation. and estimate the motion vector at finest resolution level. And then, this determined motion vector is scaled to coarser resolutions. As simulation result, this method is similar to computational complexity of the CtF MRME method and very significantly reduces that of the FtC MRME method. In addition, they provide higher quality than CtF MRME, both visually and quantitatively

• Journal of Communications and Networks
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• v.16 no.5
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• pp.523-533
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• 2014

Orthogonal frequency division multiple access (OFDMA) is a promising technique, which can provide high downlink capacity for the future wireless systems. The total capacity of OFDMA can be maximized by adaptively assigning subchannels to the user with the best gain for that subchannel, with power subsequently distributed by water-filling. In this paper, we propose the use of composite differential evolution (CoDE) algorithm to allocate the subchannels. The CoDE algorithm is population-based where a set of potential solutions evolves to approach a near-optimal solution for the problem under study. CoDE uses three trial vector generation strategies and three control parameter settings. It randomly combines them to generate trial vectors. In CoDE, three trial vectors are generated for each target vector unlike other differential evolution (DE) techniques where only a single trial vector is generated. Then the best one enters the next generation if it is better than its target vector. It is shown that the proposed method obtains higher sum capacities as compared to that obtained by previous works, with comparable computational complexity.

• Journal of Microbiology and Biotechnology
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• v.2 no.3
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• pp.174-182
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• 1992

The hemagglutinin-esterase glycoprotein (HE) gene of bovine coronavirus, coupled with a simian virus 40 early promoter and polyadenylation signal, was inserted into a human adenovirus transfer vector. The transfer vector was used to co-transfect 293 cells along with adenovirus genomic DNA. The hemagglutinin-esterase transcription unit was rescued into the adenovirus genome by homologous in vivo DNA recombination between the vector plasmid DNA and the adenovirus genomic DNA, and a recombinant adenovirus was isolated by several rounds of plaque assays. Thus the recombinant adenovirus carries the hemagglutinin-esterase gene in the early transcription region 3 (E3) of the adenovirus genome in the parallel orientation to the E3 transcription. The recombinant adenovirus synthesized the HE polypeptide in HeLa cells as demonstrated by immunoprecipitation with anti-coronavirus rabbit antisera. The recombinant HE polypeptide could be labelled by $[^3H]$glucosamine, demonstrating that the recombinant HE was glycosylated. Cells expressing the HE polypeptide exhibited hemadsorption activity when incubated with mouse erythrocytes. The HE was transported to the plasma membrane as shown by the cell surface immunofluorescence, indicating that the recombinant HE polypeptide retained its biological activities. Potential for the use of infectious recombinant adenovirus as a live virus-vectored vaccine candidate for bovine coronavirus disease is discussed.

• Steel and Composite Structures
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• v.19 no.3
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• pp.697-711
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• 2015

It is well known the importance of considering hysteretic energy demands for the seismic assessment and design of structures. In such a way that it is necessary to establish new parameters of the earthquake ground motion potential able to predict energy demands in structures. In this paper, several alternative vector-valued ground motion intensity measures (IMs) are used to estimate hysteretic energy demands in steel framed buildings under long duration narrow-band ground motions. The vectors are based on the spectral acceleration at first mode of the structure Sa($T_1$) as first component. As the second component, IMs related to peak, integral and spectral shape parameters are selected. The aim of the study is to provide new parameters or vector-valued ground motion intensities with the capacity of predicting energy demands in structures. It is concluded that spectral-shape-based vector-valued IMs have the best relation with hysteretic energy demands in steel frames subjected to narrow-band earthquake ground motions.

• Geophysics and Geophysical Exploration
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• v.25 no.1
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• pp.44-49
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• 2022

Closed-form expressions of vector gravity and gravity gradient tensor based on a line segment are derived. If a cylindrical object with axial symmetry is observed from a distance, it is possible to approximate it as a line segment; therefore, it is necessary to compute the gravity and the gravity gradient tensor due to a line source by using closed-form expressions. The gravitational potential for a line segment is defined as a one-dimensional integral, and this integral is differentiated with respect to the Cartesian coordinate system to derive the vector gravity. The expressions of the gravity gradient tensor are derived by differentiating the vector gravity once more in the same coordinate system.