• 한국미생물·생명공학회지
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• 제24권2호
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• pp.173-177
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• 1996

Bacillus thuringiensis subsp. morrisoni PG-14 is a gram-positive soil bacterium producing mosquitocidal parasporal inclusions composed of several crystal proteins. Among these crystal protein genes, cryIVD gene is one of major component which has 72 kDa in size. However, these parasporal inclusions sink quickly from the surface of water where mosquito larval feeding occurred. To develope mosquitocidal cyanobacterium, therefore, we constructed the expression vector, pCYASK 5-1 harboring cryIVD gene. The expression vector, pCYASK5-1 was transformed into the cyanobacterium Syne- chocystis PCC6803 reported as a natural mosquito larval food source and the transformants were selected with kanamycin. Expression of IVD gene in transformant was characterized by SDS-polyacrylamide gel electrophoresis (PAGE) and immunoblot analysis. The mosquitocidal activity of a transformant was determined with Culex tritaeniorhynchus. The results showed that, the transformed cyanobacterium is toxic to mosquito larvae and will be expected as a potential agent that is used for mosquito control.

• 한국임상수의학회지
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• 제29권1호
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• pp.63-67
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• 2012

Mosquitoes are the vectors of a number of viral diseases in cattle, such as Akabane disease, bovine ephemeral fever, Ainovirus infection, Chuzan virus infection, and Ibaraki disease. These diseases are transmitted from an infected animal to a non-infected host via the blood feeding of the vector. In Korea, the National Veterinary Research and Quarantine Services, Ministry for Food, Agriculture, Forestry and Fisheries is responsible for planning, implementation, laboratory investigations and reporting the results of the national surveillance program for mosquito-borne bovine diseases (MBD). The surveillance program, which was started in 1993, focused to determine the seroprevalence of each disease in cattle herds in space and time. From the epidemiological point of view, more important component of the surveillance program is to monitor infection rates in vectors for specific pathogens because this information is essential for a more precise understanding the dynamics of these diseases in a given environment and for determining risk of transmission. The aim of this study was to describe and compare methods for estimation of vector infection rates using maximum likelihood (MLE) and minimum infection rate in pooled samples. Factors affecting MLE such as number of pools, pooling size and diagnostic test performance are also discussed, assuming some hypothetical sampling scenarios for MBD.

• 대한임상검사과학회지
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• 제44권4호
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• pp.199-204
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• 2012

The purpose of this study is to examine the population density of the mosquito species, and to provide the basic data related to vector mosquitoes to manage the possible epidemic diseases. During a 4-year investigation (2001, 2002, 2004, and 2006), we collected the specimens of the mosquitoes at pier no. 1, 3 and 5 of Busan port with light traps from sunset until the next day morning from April to October each year. Through the research, we collected 5,985 mosquito specimens in total and found 7 mosquito species belonging to 3 genera. A total of 1,575, 1,473, 1,478 and 1,459 mosquito specimens were collected in 2001, 2002, 2004 and 2006, respectively. Among them, 5,568 (93.0%) specimens were Culex pipiens pallens, the West Nile virus infection vector, which showed the highest density among the specimens. The population densities of the other species were: 295(4.9%) Culex tritaeniorhynchus, 94(1.5%) Aedes togoi, 12(0.3%) Aedes albopictus, 10(0.2%) Anopheles sinensis, 4(0.1%) Aedes vexans nipponii, 2(0.1%) Culex inatomii. The monthly abundance of mosquitoes from July through September showed the highest values. Although it was a general mosquito monitoring study, this study could provide a base for securing the statistical data on spreading epidemic diseases by people infected outside Korea.

• 한국균학회지
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• 제43권2호
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• pp.77-87
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• 2015

곤충이 곰팡이병에 감염되는 것을 흔히 볼 수 있고, 대부분은 이들에 대해 감수성을 나타내고 있다. 최근 살충제 저항성이 문제가 되면서 곤충병원성 곰팡이를 이용한 모기 방제에 대한 연구가 더욱 주목을 받고 있고, Beauveria bassiana, Metarhizium anisopliae, Lagenidium giganteum와 같은 병원성 곰팡이를 모기 방제에 사용하기 위해 노력 중이다. 이 총설에서는 말라리아, 뎅기열, 사상충, 황열병 등을 옮기는 모기들의 방제에 사용되는 곤충병원성 곰팡이를 정리하고 이들의 활용방안을 논하였다.

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2004년도 춘계 학술발표회
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• pp.33-33
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• 2004

• 한국지리정보학회지
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• 제16권3호
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• pp.12-24
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• 2013

1980년대에 근절된 것으로 여겨졌던 말라리아는 재출현 이후 지난 10년간 증감을 거듭하고 있다. 우리나라의 경우에는, 휴전선에 인접한 지역이 말라리아 발생의 핫스팟으로 분류될 만큼 지역적 특징이 뚜렷하게 나타나고 있다. 특히, 휴전선 부근 시 군지역이 말라리아 발생의 위험지역으로 분류될 뿐만 아니라 지역 간 말라리아 발생률에서 차이가 있으며, 질병 발생의 중심이 변화하는 경향을 보이고 있다. 이는, 말라리아 발생이 지역별로 상이하게 펼쳐져 있는 자연 및 거주지 환경에 영향 받고 있음을 말해준다. 말라리아는 매개모기에 의해 병원체가 전달되는 것으로 이 과정은 특정 기후 및 인문환경 시스템 속에서 발현된다. 기후 요소는 매개모기와 병원체에 매우 다양하게 영향을 미치며 기온은 가장 대표적인 환경요인이다. 또한 매개모기와 인간과의 접촉 빈도와 접촉 거리는 말라리아 발생률에 가장 직접적인 영향을 미치는 요인이다. 본 연구에서는 지역별로 다른 국지적인 기후조건, 매개모기의 산란 및 서식환경의 비중, 매개모기와 거주지 간의 거리에 따라 말라리아 발생이 지역별로 차이가 나타난다는 점을 기상자료, 인공위성 기반의 토지피복 자료를 활용하여 확인하였다.

• Parasites, Hosts and Diseases
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• 제53권1호
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• pp.77-83
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• 2015

Wuchereria bancrofti, Dirofilaria immitis, and Dirofilaria repens are filarial nematodes transmitted by mosquitoes belonging to Culex, Aedes, and Anopheles genera. Screening by vector dissection is a tiresome technique. We aimed to screen filarial parasites in their vectors by single and multiplex PCR and evaluate the usefulness of multiplex PCR as a rapid xenomonitoring and simultaneous differentiation tool, in area where 3 filarial parasites are coexisting. Female mosquitoes were collected from 7 localities in Assiut Governorate, were microscopically identified and divided into pools according to their species and collection site. Detection of W. bancrofti, D. immitis, and D. repens using single PCR was reached followed by multiplex PCR. Usefulness of multiplex PCR was evaluated by testing mosquito pools to know which genera and species are used by filarial parasites as a vector. An overall estimated rate of infection (ERI) in mosquitoes was 0.6%; the highest was Culex spp. (0.47%). W. bancrofti, D. immitis, and D. repens could be simultaneously and differentially detected in infected vectors by using multiplex PCR. Out of 100 mosquito pools, 8 were positive for W. bancrofti (ERI of 0.33%) and 3 pools each were positive for D. immitis and D. repens (ERI 0.12%). The technique showed 100% sensitivity and 98% specificity. El-Nikhila, El-Matiaa villages, and Sahel Seleem district in Assiut Governorate, Egypt are still endemic foci for filarial parasites. Multiplex PCR offers a reliable procedure for molecular xenomonitoring of filariasis within their respective vectors in endemic areas. Therefore, it is recommended for evaluation of mosquito infection after lymphatic filariasis eradication programs.

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2004년도 춘계 학술발표회
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• pp.85-85
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• 2004

• Entomological Research
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• 제48권5호
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• pp.439-447
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• 2018

Adult mosquito surveillance was conducted from 2013 through 2014 at four cattle sheds, a wild bird refuge, and two residential areas located in Gyeongnam Province in the Republic of Korea. Adult mosquitoes were collected in black light traps from April 1, through November 30. Mosquito surveillance was conducted to figure out population densities of vector mosquitoes, possibly invaded mosquitoes and identify various virus infections at the selected sites. A total of 107,466 females comprising 14 species and 7 genera were collected from 2013 to 2014. The most common species collected were Culex tritaeniorhynchus Giles (63.8%), Anopheles sinensis s.l. (18.9%), Aedes vexans nipponii (Theobald) (7.7%), and Culex pipiens Coquillett (5.1%). Trap indices (TIs) varied widely for species over their range, due to geographical distribution and degree of association with rural and urban communities. The most collected An. sinensiss.l. and Cx. tritaeniorhynchus appeared at a cow shed in Hapcheon (TI 347.5) and a pigsty in Daejeo-1-dong, Busan (TI 1,040.8), respectively, due in part to their situation near breeding sites such as rice paddies. The bi-weekly population densities for mosquito species were variable for each of the years, apparently as a result of variable annual weather conditions. None of the mosquito species collected tested for the flavivirus including Japanese Encephalitis Virus, West Nile Virus, Dengue Virus, and Zika Virus infections by polymerase chain reaction (PCR) assay were positive.

• Parasites, Hosts and Diseases
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• 제40권1호
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• pp.45-54
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• 2002

Field trials evaluating selected commercially available mosquito traps variously baited with light, carbon dioxide, and/or octenol were conducted from 18-27 September 2000 in a malarious area near Paekyeon-ri (Tongil-Chon) and Camp Greaves in Paju County, Kyonggi Province, Republic of Korea. The host-seeking activity for common mosquito species, including the primary vector of Japanese encephalitis, Culex tritaeniorhynchus Giles. was determined using hourly aspirator collections from a human and propane lantern-baited Shannon trap doting hours when temperatures exceeded $15^{\circ}C$. The total number of mosquitoes and number of each species captured during the test was compared using a block design. Significant differences were observed for the total number of mosquitoes collected, such that, the Mosquito MagnetTM with octenol > Shannon trap > ABC light trap with light and dry ice > Miniature Black Light trap (manufactured by John W. Hock) $\geq$ New Jersey Trap > ABC light trap with light only. Significant differences in numbers collected among trapes were noted for several species including: Aedes vexans (Meigen), Anopheles lesteri Baisas and Hu. An. sinensis Weidemann, An. sineroides Yamada, An. yatsushiroensis Miyazaki. Culex pipiens pallets Coquillett L., Cx. orientalis Edwards and Cx. tritaeniorhynchus. Host-seeking activity for most common species showed a similar bimodal pattern. Results from these field trap evaluations can significantly enhance current vector and disease surveillance efforts especially for the primary vector of Japanese encephalitis, Cx. tritaeniorhunchus.