• IMMUNE NETWORK
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• v.4 no.1
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• pp.23-30
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• 2004

Background: A human orthologue of mouse S100A6-binding protein (CacyBP), Siah-1-interacting protein (SIP) had been shown to be a component of novel ubiquitinylation pathway regulating $\beta$-catenin degradation. The role of the protein seems to be important in cell proliferation and cancer evolution but the expression pattern of SIP in actively dividing cancer tissues has not been known. For the elucidation of the role of SIP protein in carcinogenesis, it is essential to produce monoclonal antibodies specific to the protein. Methods: cDNA sequence coding for ORF region of human SIP gene was amplified and cloned into an expression vector to produce His-tag fusion protein. Recombinant SIP protein and monoclonal antibody to the protein were produced. The N-terminal specificity of anti-SIP monoclonal antibody was conformed by immunoblot analysis and enzyme linked immunosorbent assay (ELISA). To study the relation between SIP and colon carcinogenesis, the presence of SIP protein in colon carcinoma tissues was visualized by immunostaining using the monoclonal antibody produced in this study. Results: His-tag-SIP (NSIP) recombinant protein was produced and purified. A monoclonal antibody (Korea patent pending; #2003-45296) to the protein was produced and employed to analyze the expression pattern of SIP in colon carcinoma tissues. Conclusion: The data suggested that anti-SIP monoclonal antibody produced here was valuable for the diagnosis of colon carcinoma and elucidation of the mechanism of colon carcinogenesis.

• Journal of KIISE:Software and Applications
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• v.37 no.6
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• pp.455-463
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• 2010

In this paper, we present an approach to sentence boundary detection for web documents that builds on statistical-based methods and uses rule-based correction. The proposed system uses the classification model learned offline using a training set of human-labeled web documents. The web documents have many word-spacing errors and frequently no punctuation mark that indicates the end of sentence boundary. As sentence boundary candidates, the proposed method considers every Ending Eomis as well as punctuation marks. We optimize engine performance by selecting the best feature, the best training data, and the best classification algorithm. For evaluation, we made two test sets; Set1 consisting of articles and blog documents and Set2 of web community documents. We use F-measure to compare results on a large variety of tasks, Detecting only periods as sentence boundary, our basis engine showed 96.5% in Set1 and 56.7% in Set2. We improved our basis engine by adapting features and the boundary search algorithm. For the final evaluation, we compared our adaptation engine with our basis engine in Set2. As a result, the adaptation engine obtained improvements over the basis engine by 39.6%. We proved the effectiveness of the proposed method in sentence boundary detection.

• KIPS Transactions on Software and Data Engineering
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• v.2 no.12
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• pp.889-898
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• 2013

Due to the popularization of high-performance capturing equipments and the emergence of powerful image-editing softwares, it is easy to make high-quality counterfeit money. However, the probability of detecting counterfeit money to the general public is extremely low and the detection device is expensive. In this paper, a counterfeit money detection algorithm using a general purpose scanner and computer system is proposed. First, the printing features of color printers are calculated using morphological operations and gray-level co-occurrence matrix. Then, these features are used to train a support vector machine classifier. This trained classifier is applied for identifying either original or counterfeit money. In the experiment, we measured the detection rate between the original and counterfeit money. Also, the printing source was identified. The proposed algorithm was compared with the algorithm using wiener filter to identify color printing source. The accuracy for identifying counterfeit money was 91.92%. The accuracy for identifying the printing source was over 94.5%. The results support that the proposed algorithm performs better than previous researches.

• IMMUNE NETWORK
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• v.2 no.3
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• pp.175-181
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• 2002

Background: S100A6 is a calcium-binding protein overexpressed in several tumor cell lines including melanoma with high metastatic activity and involved in various cellular processes such as cell division and differentiation. To detect S100A6 protein in patient' samples (ex, blood or tissue), it is essential to produce a monoclonal antibody specific to the protein. Methods: First, cDNA coding for ORF region of human S100A6 gene was amplified and cloned into the expression vector for GST fusion protein. We have produced recombinant S100A6 protein and subsequently, monoclonal antibodies to the protein. The specificity of anti-S100A6 monoclonal antibody was confirmed using recombinant S100A recombinant proteins of other S100A family (GST-S100A1, GST-S100A2 and GST-S100A4) and the cell lysates of several human cell lines. Also, to identify the specific recognition site of the monoclonal antibody, we have performed the immunoblot analysis with serially deleted S100A6 recombinant proteins. Results: GST-S100A6 recombinant protein was induced and purified. And then S100A6 protein excluding GST protein was obtained and monoclonal antibody to the protein was produced. Monoclonal antibody (K02C12-1; patent number, 330311) has no cross-reaction to several other S100 family proteins. It appears that anti-S100A6 monoclonal antibody reacts with the region containing the amino acid sequence from 46 to 61 of S100A6 protein. Conclusion: These data suggest that anti-S100A6 monoclonal antibody produced can be very useful in development of diagnostic system for S100A6 protein.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.37 no.5
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• pp.54-63
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• 2000

We present a multi-resolution block matching algorithm (BMA) for fast motion estimation At the coarsest level, a motion vector (MV) having minimum matching error is chosen via a full search, and a MV with minimum matching error is concurrently found among the MVs of the spatially adjacent blocks Here, to examine the spatial MVs accurately, we propose an efficient method for searching full resolution MV s without MV quantization even at the coarsest level The chosen two MV s are used as the initial search centers at the middle level At the middle level, the local search is performed within much smaller search area around each search center If the method used at the coarsest level is adopted here, the local searches can be done at integer-pel accuracy A MV having minimum matching error is selected within the local search areas, and then the final level search is performed around this initial search center Since the local searches are performed at integer-pel accuracy at the middle level, the local search at the finest level does not take an effect on the overall performance So we can skip the final level search without performance degradation, thereby the search speed increases Simulation results show that in comparison with full search BMA, the proposed BMA without the final level search achieves a speed-up factor over 200 with minor PSNR degradation of 02dB at most, under a normal MPEG2 coding environment Furthermore, our scheme IS also suitable for hardware implementation due to regular data-flow.

• Korean Journal of Agricultural Science
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• v.45 no.1
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• pp.38-42
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• 2018

Demand for tomatoes has been increasing every year as people desire more healthy food. In Korea, tomatoes are mainly grown in the Chungnam, Chunnam and Kyungnam provinces. Recently, reports of whitefly-transmitted viral diseases have increased due to newly emerging whitefly pressures caused by climate change in Korea. Specifically, in 2017, the main tomato growing areas, Buyeo and Nonsan in Chungnam, showed damage typical of viral infection; therefore, we investigated viral diseases in these areas. We collected samples with virus-like symptoms and found that not only whitefly transmitted Tomato yellow leaf curl virus (TYLCV) and Tomato chlorosis virus (ToCV) were detected but also Tomato mosaic virus (ToMV, for which no specific vector is known) and Tomato spotted wilt virus (TSWV, transmitted by thrips). The ToMV-infected samples were mostly co-infected with either TYLCV or ToCV. Mixed infections of different combinations of TYLCV, ToCV and ToMV were detected with the mixed infection of two whitefly-transmitted viruses (TYLCV and ToCV) causing the most severe symptoms. According to the CP sequence of each virus, the 100% identities were shown to be Mexico/ABG73017.1 (TYLCV), Greece/CDG34553.1 (ToCV), China/AKN79752 (TSWV), and Australia/NP078449.1 (ToMV). Based on the sequence data, we presumed that these tomato infecting viruses were transmitted through insects and seeds introduced from neighboring countries.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.11 no.1
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• pp.53-62
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• 2017

In this paper, we present the score classification accuracy of BBS(Berg Balance Scale) which is the most commonly used balance evaluation tool using machine learning. Data acquisition was performed using the Noraxon system and an inertial sensor of Noraxon system was attached to the body in 8 locations (left and right ankle, left and right upper buttocks, left and right wrists, back, forehead). Based on the 3-axis accelerometer of the inertial sensor, the feature vector STFT(Short Time Fourier Transform) and SAM(Signal Area Magnitude) were extracted. Then, the items of the BBS were divided into static movement and dynamic movement depending on the operation characteristics, and the feature vectors were selected according to the sensor attachment positions which affect the score for each item of the BBS. Feature vectors selected for each item of BBS were classified using GMM(Gaussian Mixture Model). As a result of the accuracy calculation for 40 subjects, 55.5%, 72.2%, 87.5%, 50%, 35.1%, 62.5%, 43.3%, 58.6%, 60.7%, 33.3%, 44.8%, 89.2%, 51.8%, 85.1%, respectively.

• The Korean Journal of Malacology
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• v.30 no.4
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• pp.429-431
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• 2014

Since we reported a BLAST server for the mollusk in 2004, no work has reported the usability or modification of the server. To improve its usability, the BLAST server for the mollusk has been updated as version II (http://www.malacol.or.kr/blast) in the present study. The database was constructed by using the Intel server Platform ZSS130 dual Xeon 3.20 GHz CPU and Linux CentOS system and with NCBI WebBLAST package. We downloaded the mollusk nucleotide, amino acid, EST, GSS and mitochondrial genome sequences which can be opened through NCBI web BLAST and used them to build up the database. The updated database consists of 520,977 nucleotide sequences, 229,857 amino acid sequences, 586,498 EST sequences, 23,112 GSS and 565 mitochondrial genome sequences. Total database size is 1.2 GB. Furthermore, we have added repeat sequences, Escherichia coli sequences and vector sequences to facilitate data validation. The newly updated BLAST server for the mollusk will be useful for many malacological researchers as it will save time to identify and study various molluscan genes.

• Journal of Korea Spatial Information System Society
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• v.1 no.2 s.2
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• pp.21-31
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• 1999

In accordance with the completion of the spatial database building works in the central and/or local government authorities as well as the rapid popularization of various information services through internet, it is heavily required to provide spatial information services through World Wide Web. To provide a qualified spatial information service, it is crucial to have a Web-based GIS (Geographic Information Service) service system equipped with the publicity, the convenient accessibility, and the easy-to-use user interface. In this paper, we introduce a new component system for Web-based spatial information services based on the OpenGIS Simple Feature specification for OLE/COM[3] and OLE DB specification[4]. The important functionality of a Web-based spatial information service system includes its accessibility of various existing GIS server system and huge databases in addition to the resolution of response-time delay problems caused by transmitting a large amount of digital maps via internet[6]. To cope with these problems, our component system has been designed to access heterogeneous databases in transparent manner and to support vector-based and/or image-based image production techniques for shortening transmission time.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.86-86
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• 2003

Research has been in progress for more than a decade to production of useful proteins by genetic modification in cattle. However, the levels of protein production in transgenic cattle have been reported very low. To enhance protein production in transgenic animal, we tried homologous recombination to donor cells for production of transgenic clone cattle through nuclear transfer procedure. Thus, we constructed the two targeting vectors of human thrombopoietin (TPO) at bovine $\beta$-casein locus using homologous recombination with 13.6 kb and 9.6 kb homology. In two targeting vectors, positive selection was through the neomycin resistance gene and negative selection was by the diphtheria toxin (DT). Gene targeting was attempted in bovine embryonic fibroblasts (bEF) and bovine ear skin fibroblasts (bESF). To determine the most appropriate concentration of neomycin for bEF and bESF, G4l8 resistance was confirmed by culturing the cells in various concentrations of the drug and both of the cells were optimally selected at $900 \mu g/ml$ of neomycin. The transfected bEF and bESF by the targeting vectors were colonized efficiently at the ratio of DNA to transfection reagent such as $4 \mu g$:2 ${mu}ell$ and $1 \mu g$:$2 \mu l$. Comparing number of healthy clones from passage 4 to passage 8, bESF (17%) persist in culture for much longer than bEF (6%). The two gene-targeted bESF clones of 30 random-integrated clones with 9.6 kb homology length were confirmed, however, nothing was out of 72 random integration clones with 13.6 kb homology length, The DT also worked more efficiently in clones transfected with the vector of 9.6 kb homology length. Our data suggests that the choice of donor cell for long culture period should be considered to obtain targeted cell clone, and the gene-targeting frequency and the DT working efficiency are dependent on the length of target homology.