• Journal of Microbiology
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• v.44 no.5
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• pp.548-555
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• 2006

FaeG is the key factor in the infection process of K88ad enterotoxigenic Escherichia coli (ETEC) fimbrial adhesin. In an attempt to determine the possibility of expressing recombinant FaeG with immunogenicity for a new safe and high-production vaccine in E. coli, we constructed the recombinant strain, BL21 (DE3+K88), which harbors an expression vector with a DNA fragment of faeG, without a signal peptide. Results of 15% SDS-polyacrylamide slab gel analysis showed that FaeG can be stably over-expressed in BL21 (DE3+K88) as inclusion bodies without FaeE. Immunoglobulin G (IgG) and M (IgM) responses in pregnant pigs, with boost injections of the purified recombinant FaeG, were detected 4 weeks later in the sera and colostrum. An in vitro villius-adhesion assay verified that the elicited antibodies in the sera of vaccinated pigs were capable of preventing the adhesion of K88ad ETEC to porcine intestinal receptors. The protective effect on the mortality rates of suckling piglets born to vaccinated mothers was also observed one week after oral challenge with the virulent ETEC strain, $C_{83907}$ (K88ad, $CT^+,\;ST^+$). The results of this study proved that the adhesin of proteinaceous bacterial fimbriae or pili could be overexpressed in engineered E. coli strains, with protective immune responses to the pathogen.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.39C no.10
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• pp.948-956
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• 2014

Today, Intelligent Vehicle Detection System seeks to reduce the negative factors, such as accidents over to get the traffic information of existing system. This paper proposes detection algorithm for the illegal U-turn vehicles which can cause critical accident among violations of road traffic laws. We predicted that if calculated optical flow vectors were shown on the illegal U-turn path, they would be cause of the illegal U-turn vehicles. To reduce the high computational complexity, we use the algorithm of pyramid Lucas-Kanade. This algorithm only track the key-points likely corners. Because of the high computational complexity, we detect center lane first through the color information and progressive probabilistic hough transform and apply to the around of center lane. And then we select vectors on illegal U-turn path and calculate reliability to check whether vectors is cause of the illegal U-turn vehicles or not. Finally, In order to evaluate the algorithm, we calculate process time of the type of algorithm and prove that proposed algorithm is efficiently.

• IMMUNE NETWORK
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• v.4 no.1
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• pp.23-30
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• 2004

Background: A human orthologue of mouse S100A6-binding protein (CacyBP), Siah-1-interacting protein (SIP) had been shown to be a component of novel ubiquitinylation pathway regulating $\beta$-catenin degradation. The role of the protein seems to be important in cell proliferation and cancer evolution but the expression pattern of SIP in actively dividing cancer tissues has not been known. For the elucidation of the role of SIP protein in carcinogenesis, it is essential to produce monoclonal antibodies specific to the protein. Methods: cDNA sequence coding for ORF region of human SIP gene was amplified and cloned into an expression vector to produce His-tag fusion protein. Recombinant SIP protein and monoclonal antibody to the protein were produced. The N-terminal specificity of anti-SIP monoclonal antibody was conformed by immunoblot analysis and enzyme linked immunosorbent assay (ELISA). To study the relation between SIP and colon carcinogenesis, the presence of SIP protein in colon carcinoma tissues was visualized by immunostaining using the monoclonal antibody produced in this study. Results: His-tag-SIP (NSIP) recombinant protein was produced and purified. A monoclonal antibody (Korea patent pending; #2003-45296) to the protein was produced and employed to analyze the expression pattern of SIP in colon carcinoma tissues. Conclusion: The data suggested that anti-SIP monoclonal antibody produced here was valuable for the diagnosis of colon carcinoma and elucidation of the mechanism of colon carcinogenesis.

• Journal of the Korea Computer Graphics Society
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• v.16 no.1
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• pp.1-7
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• 2010

With the widespread use of vision-based surveillance systems, the capability for person identification is now an essential component. However, the CCTV cameras used in surveillance systems tend to produce relatively low-resolution images, making it difficult to use face recognition techniques for person identification. Therefore, an algorithm is proposed for person identification in CCTV camera images based on the clothing. Whenever a person is authenticated at the main entrance of a building, the clothing feature of that person is extracted and added to the database. Using a given image, the clothing area is detected using background subtraction and skin color detection techniques. The clothing feature vector is then composed of textural and color features of the clothing region, where the textural feature is extracted based on a local edge histogram, while the color feature is extracted using octree-based quantization of a color map. When given a query image, the person can then be identified by finding the most similar clothing feature from the database, where the Euclidean distance is used as the similarity measure. Experimental results show an 80% success rate for person identification with the proposed algorithm, and only a 43% success rate when using face recognition.

• The Journal of Engineering Geology
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• v.23 no.1
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• pp.47-55
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• 2013

To analyze lateral displacement of excavation walls exposed during the construction of Subway Line 1 in the Daegu region, inclinometer measurement data for sites D4, D5, and Y6 are investigated from the perspective of engineering geology. The study area, in the Banyawol Formation, Hayang Group, Gyeongsang Supergroup, is in the lower part of bedrock of andesitic volcanics, calcareous shale, sandstone, hornfels, and felsite dykes that are unconformably overlain by soil. The rock mass around the D4 site is classified as RMR-V grade and the maximum lateral displacement of 101.39 mm, toward N34W, was measured at a bedding-parallel fault, at a depth of 12 m. The rock mass around the D5 site is classified as RMR-IV grade and the maximum lateral displacement of 55.17 mm, toward the south, was measured at a lithologic contact between shale and felsite, at a depth of 14 m. The rock mass around the Y6 site is classified as RMR-III grade and the maximum lateral displacement of 12.65 mm, toward S52W, was measured at an unconformity between the soil and underlying bedrocks, at a depth of 7 m. The directions of lateral displacement in the excavation walls are vector sums of the directions perpendicular to the excavation wall and horizontally parallel to the excavation wall. Lateral displacement graphs according to depth in the soil profile show curvilinear trajectories, whereas those in bedrock show straight and rapid-displacement trajectories.

• Journal of Life Science
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• v.21 no.10
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• pp.1370-1376
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• 2011

To study the functional genomic analysis of a crenachaeon Sulfolobus acidocaldarius, we have constructed an auxotrophic mutant based on pyrEF, which encodes the pyrimidine biosynthetic enzymes orotate phosphoribosyltransferase and orotidine-5'-monophosphate decarboxylase. S. acidocaldarius was shown to be sensitive to 5-fluoroorotic acid (5-FOA), which can be selected for mutations in pyrEF genes within a pyrimidine biosynthesis cluster. Spontaneous 5-FOA-resistant mutants by ultraviolet, KH1U and KH2U, were found to contain two point mutations and a frame shift mutation in pyrE, respectively. Mutations at these sites from KH1U and KH2U decreased the activity of orotate phosphoribosyltransferase encoded by the pyrE gene and blocked the degradation of 5-FOA into toxic 5-FOMP and 5-FUMP that kill the cells. Therefore, KH1U and KH2U were uracil auxotrophs. Transformation of Sulfolobus-Escherichia coli shuttle vector pC bearing pyrEF genes from S. solfataricus P2 into S. acidocaldarius mutant KH2U restored 5-FOA sensitivity and overcame the uracil auxotrophy. This study establishes an efficient genetic strategy towards the systematic knockout of genes in S. acidocaldarius.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.3
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• pp.292-301
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• 2010

Follicle-stimulating hormone (FSH) is a pituitary glycoprotein hormone that is encoded by separate alpha- and betasubunit genes. It plays a key role in stimulating and regulating ovarian follicular development and egg production in chicken. FSH signal transduction is mediated by the FSH receptor (FSHR) that exclusively interacts with the beta-subunit of FSH, but characterization of prokaryotic expression of the FSHb gene and its effect on the expression of the FSHR gene in local chickens have received very little attention. In the current study, the cDNA fragment of the FSHb gene from Dagu chicken was amplified using reverse transcription polymerase chain reaction (RT-PCR), and inserted into the pET-28a (+) vector to construct the pET-28a-FSHb plasmid. After expression of the plasmid in E. coli BL21 (DE3) under inducing conditions, the recombination protein, $FSH{\beta}$ subunit, was purified and injected into the experimental hens and the effect on the mRNA expression levels of the FSHR gene was investigated. Sequence comparison showed that the coding region of the FSHb gene in the local chicken shared 99%-100% homology to published nucleotides in chickens; only one synonymous nucleotide substitution was detected in the region. The encoded amino acids were completely identical with the reported sequence, which confirmed that the sequences of the chicken FSHb gene and the peptides of the $FSH{\beta}$ subunit are highly conserved. This may be due to the critical role of the normal function of the FSHb gene in hormonal specificity and regulation of reproduction. The results of gene expression revealed that a recombinant protein with a molecular weight of about 19 kDa was efficiently expressed and it was identified by Western blotting analysis. After administration of the purified $FSH{\beta}$ protein, significantly higher expression levels were demonstrated in uterus, ovary and oviduct samples (p<0.05). These observations suggested that the expressed $FSH{\beta}$ protein possesses biological activity, and has a potential role in regulation of reproductive physiology in chickens.

• Journal of the Korean Association of Geographic Information Studies
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• v.14 no.1
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• pp.84-93
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• 2011

Levee line mapping is critical to the protection of environments in river zones, the prevention of river flood and the development of river zones. Use of the remote sensing data such as LiDAR and aerial orthoimage is efficient for river mapping due to their accessibility and higher accuracy in horizontal and vertical direction. Airborne laser scanning (LiDAR) has been used for river zone mapping due to its ability to penetrate shallow water and its high vertical accuracy. Use of image source is also efficient for extraction of features by analysis of its image source. Therefore, aerial orthoimage also have been used for river zone mapping tasks due to its image source and its higher accuracy in horizontal direction. Due to these advantages, in this paper, research on three dimensional levee line mapping is implemented using LiDAR and aerial orthoimage separately. Accuracy measurement is implemented for both extracted lines generated by each data using the ground truths and statistical comparison is implemented between two measurement results. Statistical results show that the generated 3D levee line using LiDAR data has higher accuracy than the generated 3D levee line using aerial orthoimage in horizontal direction and vertical direction.

• IMMUNE NETWORK
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• v.2 no.3
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• pp.175-181
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• 2002

Background: S100A6 is a calcium-binding protein overexpressed in several tumor cell lines including melanoma with high metastatic activity and involved in various cellular processes such as cell division and differentiation. To detect S100A6 protein in patient' samples (ex, blood or tissue), it is essential to produce a monoclonal antibody specific to the protein. Methods: First, cDNA coding for ORF region of human S100A6 gene was amplified and cloned into the expression vector for GST fusion protein. We have produced recombinant S100A6 protein and subsequently, monoclonal antibodies to the protein. The specificity of anti-S100A6 monoclonal antibody was confirmed using recombinant S100A recombinant proteins of other S100A family (GST-S100A1, GST-S100A2 and GST-S100A4) and the cell lysates of several human cell lines. Also, to identify the specific recognition site of the monoclonal antibody, we have performed the immunoblot analysis with serially deleted S100A6 recombinant proteins. Results: GST-S100A6 recombinant protein was induced and purified. And then S100A6 protein excluding GST protein was obtained and monoclonal antibody to the protein was produced. Monoclonal antibody (K02C12-1; patent number, 330311) has no cross-reaction to several other S100 family proteins. It appears that anti-S100A6 monoclonal antibody reacts with the region containing the amino acid sequence from 46 to 61 of S100A6 protein. Conclusion: These data suggest that anti-S100A6 monoclonal antibody produced can be very useful in development of diagnostic system for S100A6 protein.

• Journal of Digital Convergence
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• v.15 no.11
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• pp.245-250
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• 2017

Flash floods is defined as the flooding of intense rainfall over a relatively small area that flows through river and valley rapidly in short time with no advance warning. So that it can cause damage property and casuality. This study is to establish the flash-flood warning system using 38 accident data, reported from the National Disaster Information Center and Land Surface Model(TOPLATS) between 2009 and 2012. Three variables were used in the Land Surface Model: precipitation, soil moisture, and surface runoff. The three variables of 6 hours preceding flash flood were reduced to 3 factors through factor analysis. Decision tree, random forest, Naive Bayes, Support Vector Machine, and logistic regression model are considered as big data methods. The prediction performance was evaluated by comparison of Accuracy, Kappa, TP Rate, FP Rate and F-Measure. The best method was suggested based on reproducibility evaluation at the each points of flash flood occurrence and predicted count versus actual count using 4 years data.