• KSBB Journal
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• v.23 no.5
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• pp.403-407
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• 2008

Recently, Candida antarctica lipase B (CalB) draws attention from industries for various applications for food, detergent, fine chemical, and biodiesel, because of its characteristics as an efficient biocatalyst. Since many industrial processes carry out in organic solvent and at high temperature, CalB, which is stable under harsh condition, is in demand from many industries. In order to reform CalB promptly, the expression system which has advantages of ease to use and low cost for gene libraries screening was developed using E. coli. The E. coli strains, Rosettagami with competence for enhanced disulfide bond formation, Novablue, and $DH5{\alpha}$, were exploited in this study. To obtain the soluble CalB, the pCold I vector expressing the cloned gene at $15^{\circ}C$ and the chaperone plasmids containing groES/groEL, groES/groEL/tig, tig, dnaK/dnaJ/grpE, and dnaK/dnaJ/grpE/groES/groEL were used for coexpression of CalB and chaperones. The colonies expressing functional lipase were selected by employing the halo plate containing 1% tributyrin, and the CalB expression was confirmed by SDS-PAGE. E. coli Rosettagami and $DH5{\alpha}$ harbouring groES/groEL chaperones were able to express soluble CalB effectively. From a facilitative point of view, E. coli $DH5{\alpha}$ is more suitable for further mutation study.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.37 no.2
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• pp.303-310
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• 2017

Evapotranspiration is a significant hydrologic quantity for understanding the amount of available water resource evaluation, water balance analysis, water circulation and energy circulation. Various methods have been developed for estimating the evapotranspiration using data observed at meteorological observatories. Especially, the focus of methods has been on the complementary relationship that the actual evapotranspiration is equal to the difference between the twice of evapotranspiration in the wet condition and the potential evapotranspiration. The Granger and Gary (GG) method is an empirical formula that can be used to estimate the evapotranspiration using only empirical parameters based on the complementary relationship and using only the net radiation and temperature of the region. In this study, we compared the evapotranspiration data observed at 10 sites in Asia within the dataset of FLUXNET2015, with the evapotranspiration calculated by GG method. The evapotranspiration in inland area was estimated more accurately than that of coastal area. Simulated Annealing (SA) was used for the coastal area to modify the parameters. Using the modified GG method, we could improve the statistics such as root mean square error, the coefficient of determination ($R^2$), and the mean absolute ${\mid}BIAS{\mid}$ of the evapotranspiration estimation in coastal area.

• Korean Journal of Food Science and Technology
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• v.52 no.5
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• pp.510-515
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• 2020

In recent years, there has been an increasing interest in research on gluten-free bread. Most studies have focused on substituting or imitating the gluten network. However, less attention has been paid to technological approaches to improve the quality of gluten-free rice bread (GFRB). This study analyzed the influence of the temperature of water used for kneading and fermentation time on the quality of GFRB. Water at various amounts and different temperatures was added for kneading, and fermentation was performed for 0, 2, and 4 h. GFRB produced by kneading using water at 95℃ showed the highest specific volume and the lowest hardness among test groups, regardless of other factors. In conclusion, mixing equal volumes of water at 95℃ and rice flour, followed by a 2 h fermentation process, produced the best-quality GFRB in terms of cross-sectional area, hardness, and appearance.

• Journal of the Korean Wood Science and Technology
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• v.45 no.1
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• pp.107-115
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• 2017

In this study, the surface check reduction effect of preservative-treated Korean larch round-wood was investigated by applying three physical treatments, such as incising, kerfing, and kiln-drying. Moreover, the possibility of long-term service life was also checked by comparing the depth of surface check and the penetration depth of preservative. A rapid gradient of wood moisture content between surface and center causes a surface check. Thus, the effect of reducing surface check was carried out at the lowest equilibrium moisture content (EMC) condition, temperature ($2^{\circ}C$), humidity (44.6%), in outdoor locations in Korea until the test specimens reach to the 8.6% EMC. As a result, the preservative-treated specimens without incising, kerfing, kiln-drying (Type C) could not ensure the long-term service life due to a large surface check. Because the surface check depth of all specimens was deeper than the penetration depth of preservative. In case of the incising treated specimens (Type I), 80% of them, the depth of surface check was not deeper than the penetration depth of preservative. However, when a kerfing was additionally treated (Type B), a possibility of happening deeper surface check than penetration depth of preservative was increased rather than Type I.

• Korean Journal of Materials Research
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• v.21 no.2
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• pp.111-114
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• 2011

As a growth-template of ZnO nanorods (NR), a hexagonal $\beta-Ni(OH)_2$ nanosheet (NS) was synthesized with the low temperature hydrothermal process and its microstructure was investigated using a high resolution scanning electron microscope and transmission electron microscope. Zinc nitrate hexahydrate was hydrolyzed by hexamethylenetetramine with the same mole ratio and various temperatures, growth times and total concentrations. The optimum hydrothermal processing condition for the best crystallinity of hexagonal $\beta-Ni(OH)_2$ NS was determined to be with 3.5 mM at $95^{\circ}C$ for 2 h. The prepared $Ni(OH)_2$ NSs were two dimensionally arrayed on a substrate using an air-water interface tapping method, and the quality of the array was evaluated using an X-ray diffractometer. Because of the similarity of the lattice parameter of the (0001) plane between ZnO (wurzite a = 0.325 nm, c = 0.521 nm) and hexagonal $\beta-Ni(OH)_2$ (brucite a = 0.313 nm, c = 0.461 nm) on the synthesized hexagonal $\beta-Ni(OH)_2$ NS, ZnO NRs were successfully grown without seeds. At 35 mM of divalent Zn ion, the entire hexagonal $\beta-Ni(OH)_2$ NSs were covered with ZnO NRs, and this result implies the possibility that ZnO NR can be grown epitaxially on hexagonal $\beta-Ni(OH)_2$ NS by a soluble process. After the thermal annealing process, $\beta-Ni(OH)_2$ changed into NiO, which has the property of a p-type semiconductor, and then ZnO and NiO formed a p-n junction for a large area light emitting diode.

• Journal of Aquaculture
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• v.19 no.1
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• pp.47-51
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• 2006

Experiments were performed to find out the physico-chemical properties of milt and sperm motility in various storage conditions using starry flounder (Platichthys stellatus). The average sperm concentration and spermatocrit in stripped milt were $6.00{\pm}0.98{\times}10^8/mL\;and\;72{\pm}5$, respectively. The osmolality and pH were $337{\pm}9mmol/kg$ and $7.7{\pm}0.1$, respectively. The sperm of starry flounder was preserved with artificial seminal plasma (ASP), Stein's solution (SS) and marine fish Ringer's solution (MFRS) at $0^{\circ}C,\;2^{\circ}C,\;and\;4^{\circ}C$. The most effective condition for cold storage was SS at $0^{\circ}C$, and the preserved sperm remained motile for 30 days.

• International Journal of Highway Engineering
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• v.9 no.1 s.31
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• pp.1-15
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• 2007

The objective of this paper is the establishment of finite element analysis frame work for pavement research. Finite element analysis results simulating various loading experiments are verified with sensor measurements obtained from the KHC Test Road. The accuracy of the finite element analysis can be supported by these efforts so that it helps spread out the finite element analysis to pavement research and design processes. The finite element model used in this research is the full 3D nonlinear model including concrete slab, lean concrete base, subbase, shoulder, dowel, and tie-bar. In order to accomplish the accurate verification, the loading condition and the pavement temperature distribution are exactly simulated with field measured data. The curling behavior and the strain distribution are compared with measured responses from the loading tests with a truck and the FWD. Strain and curling predictions from the concrete slab are matched well with measured responses but the strain prediction from the lean concrete base is not matched with measured response. In addition, the magnitude of permanent curling is evaluated with the finite element analysis.

• Journal of the Mineralogical Society of Korea
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• v.18 no.4 s.46
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• pp.267-276
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• 2005

The study is to determine the feasibility of activated red mud as an anti-red tide material. The red mud, a byproduct of Bayer process for the production of alumina from bauxite, contained hematite, boehmite, calcite, sodalite, quartz, zircon, anatase and an unknown phase. In the adsorption study of the red mud, its adsorption efficiencies for heavy elements were close to $100\%$ except $92\%$ In As. These results seem to be attributed by the high adsorption ability of iron oxides for heavy elements. As a result of leaching tests with the red mud at various pHs (pH $1\∼13$), the high leaching efficiencies for As, Cu and Zn at low pHs (at acidic condition) were obtained. It indicated that removal efficiency of heavy elements could be excellent in acidic treatment of red mud. The activated red mud, red mud reacted with acid, contained hematite, boehmite and so on, and desorption of heavy metals from the activated red mud increased with increasing temperature. The grain of the activated red mud was tens nm in size. The removal efficiency for 5 types of plankton was generally in inverse proportion to pH, especially to final pH. Of five plankton types, Prorocentrum minimum and Alexandrium tamarense promptly were removed more than $90\%$ as soon as the activated red mud was sprayed and $100\%$ after 30 minutes. These results indicated that the activated red mud seems to be a promising anti-red tide material.

• Journal of the Korean earth science society
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• v.35 no.1
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• pp.41-53
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• 2014

The special observation using Radiosonde was performed to investigate precipitation events over the east coast of Korea during the winter season from 5 January to 29 February 2012. This analysis focused on the various indices to describe the characteristics of the atmospheric instability. Equivalent Potential Temperature (EPT) from surface (1000 hPa) to middle level (near 750 hPa) was increased when the precipitation occurred and these levels (1000~750 hPa) had moisture enough to cause the instability of atmosphere. The temporal evolution of Convective Available Potential Energy (CAPE) appeared to be enhanced when the precipitation fell. Similar behavior was also observed for the temporal evolution of Storm Relative Helicity (SRH), indicating that it had a higher value during the precipitation events. To understand a detailed structure of atmospheric condition for the formation of precipitation, the surface remote sensing data and Automatic Weather System (AWS) data were analyzed. We calculated the Total Precipitable Water FLUX (TPWFLUX) using TPW and wind vector. TPWFLUX and precipitation amount showed a statistically significant relationship in the north easterly winds. The result suggested that understanding of the dynamical processes such as wind direction be important to comprehend precipitation phenomenon in the east coast of Korea.

• Journal of the Korea Organic Resources Recycling Association
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• v.25 no.1
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• pp.15-21
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• 2017

In the present work, the synergistic effect of seaweed addition on organic acid production from sludge was investigated. The batch experiment was conducted at various mixing ratios of sewage sludge and seaweed (100:0, 75:25, 50:50, 25:75, 0:100 on a COD basis) under the substrate concentration of 20 g COD/L. The fermentation temperature was conducted under mesophilic condition ($35^{\circ}C$) and a heat-treated ($90^{\circ}C$ for 20 min) anaerobic digester sludge was used as a seeding source to suppress the methanogenic activity, The results showed that the amount of organic acid production increased as the content of seaweed increased: organic acids were 1.45, 3.22, 4.28, 5.24 and 4.82 g COD/L for the mixing ratio of 100:0, 75:25, 50:50, 25:75 and 0:100 respectively. The synergistic effect was calculated based on the organic acid production of individual sludge and seaweed, and was found to be 0.92, 1.14, 1.26 g COD/L at the mixing ratio of 75:25, 50:50 and 25:75, which indicates that 40% of synergy was obtained when 25% of seaweed was added. The synergistic effect could be ascribed to the high C/N ratio and biodegradability of seaweed.