• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.3
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• pp.306-316
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• 1997

Embryogenic calli were induced from mature seed scutella of anther culture-derived rice variety Zhonghua 8. Cell suspension cultures were initiated from friable embryogenic calli and utilized as source material for protoplast isolation. Generally, the older and finer cell suspensions gave higher protoplast yields than younger suspension cultures. Protoplasts exhibited sustained cell division and formed microcalli when cultured in KPR medium supplemented with 0.5 mg $l^{-1}$ 2,4-D, 1.0 mg $l^{-1}$ NAA and 0.5 mg $l^{-1}$ zeatin using the agarose embedding procedure without feeder cells. Protoplast plating efficiencies ranged from 0.20 to 0.54％. Microcalli were transferred to MS medium supplemented with 2.0 mg $l^{-1}$ kinetin and 0.5mg $l^{-1}$ NAA for plant regeneration. The regeneration frequencies were 2 to 12％, depending on the cell suspension lines of Zhonghua 8. The plants were transferred to the glasshouse and were fertile.

• Food Science and Biotechnology
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• v.16 no.1
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• pp.163-166
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• 2007

The cytotoxic effects of partially purified substances from Bacillus polylfermenticus SCD toward a variety tumor cell lines were studied. Cytotoxic activity was determined with regard to the A549 (human lung carcinoma), AGS (human stomach adenocarcinoma), DLD-1 (human colon adenocarcinoma), HEC-1-B (human uterus adenocarcinoma), SW-156 (human kidney carcinoma), and NIH/3T3 (murine normal fibroblast) cell lines using the MTT assay. Cytotoxic substances were partially purified through Diaion HP-20 columns and extracted with methanol or other organic solvents (n-hexane, chloroform, ethylacetate, and butanol). B. polyfermenticus SCD supernatant showed up to 60% inhibition of cell viability fer all five human cancer cell lines tested. When treated with 10 mg/mL of n-hexane, chloroform, ethylacetate, and butanol extract, HEC-1-B cells showed a 25,62,35, and 63% rate of inhibition respectively, and AGS cells showed a 72, 61, 44, and 67% rate of inhibition, respectively. At a concentration of 10 mg/mL, 100% methanol Diaion HP-20 extracts showed inhibition rates of 97.0% toward A-549 cells, 98.1% toward AGS cells, 81.6% toward DLD-1 cells, 83.5% toward HEC-1-B cells, and 92.7% toward SW-156 cells. These results indicate that partially purified fractions from B. polyfermenticus SCD have the potential to inhibit not only colon cancer cells, but also lung, stomach uterus, and kidney cancer cells. Further studies are needed to characterize the cytotoxic substances released in B. polyfermenticus SCD cultures.

• Culinary science and hospitality research
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• v.16 no.3
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• pp.32-50
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• 2010

The empirical objectives of this study include, firstly, to identify the underlying dimensions in food tourism, secondly, to identify and test the tourists' characteristic variables that explain participation in food tourism, and finally to examine the relationship between food tourism and the characteristic variables. Derived from existing food tourism literature, three underlying dimensions of activities in food tourism are given; (1) dining at restaurants serving local cuisine, (2) purchasing local food products, and (3) dining at high quality restaurants. Four valid conceptual variables were used to test whether there are significant relationships between them and food tourism variables. They are food neophobia, variety-seeking, hedonic consumption, and identity affirmation. Based on the survey responses from 164 tourists visiting Kanghwa-do, Namisum, and Yongjong-do, multi-regression analysis was employed. The findings suggested that there were negative relationships between food neophobia and all other dimensions in food tourism. In addition, variety seeking, identity affirmation, and hedonism have positive influence on the dimensions of food tourism. Therefore, utilizing various culinary cultures, food tourism activities with increasing the availability and branding of indigenous local dishes are strongly advised to the destinations concentrating in the food tourism market.

• Korean Institute of Interior Design Journal
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• v.18 no.4
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• pp.88-96
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• 2009

Contemporary hotels adopt distinctive designs in order to symbolize themes of various spaces they consist of. This phenomenon shows that the importance of spiritual values reflected through themes of spaces is emphasized as much as those of basic functions and structures of hotels. Above all, a variety of expression methods pervading throughout spaces of guest rooms consist of thematic spaces, visualization of emotions, as well as mixture of artistic genres, ones that are originated from unconsciousness of symbolic theory of C.G. Jung and widely perceived as variations of archetype. Meanings of symbolic expressions derived from the analysis of various hotels can be defined by attributes of local cultures as well as propensities of mythicism, which feature local pride enlightened by natural environments, the spirit of the times, historical events and other transcendental and fantastic topics. These symbolic expressions are metaphysical forms that are made unconsciously and such symbols are far beyond mere historical and cultural signs which require us profound and mature methods to approach. It is pleasing to know that we are living in the modern society in which progresses are being made to better understand minds and metal states of human beings. Given such a circumstantial advantage, researches on meanings of symbolic expressions should not be limited to only those of hotel designs, but also those of religious architectures and museums in which spiritual values are emphasized throughout designs of the spaces they consist of.

• Journal of Dairy Science and Biotechnology
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• v.18 no.1
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• pp.47-60
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• 2000

Over decades of work, the probiotic research has grown rapidly with a number of new cultures, which is claimed a variety of benefit. However, many of the specific effects attributed to the ingestion of probiotics remain convoluted and scientifically unsubstantiated. Accordingly, the scientific community faces a greater challenge and must objectively seek cause and effect relationships for many potential and currently investigated probiotic species. Rational selection and design of probiotics remains an important challenge and will require a solid information about the physiology and genetics of candidate strains relevant to their intestinal roles, functional activities, and interaction of with other resident micro flora. As far as beneficial culture of lactic acid bacteria (LAB) is concerned, simple, cost-effective, and exact identification of candidate strains is of foremost importance among others. Until recently, the relatedness of bacterial isolates has been determined sorely by testing for one or several phenotyphic markers, using methods such as serotyping, phage-typing, biotyping, and so forth. However, there are problems in the use of many of these phenotype-based methods. In contrast, some of newer molecular typing methods involving the analysis of DNA offer many advantages over traditional techniques. These DNA-based methods have the greater discriminatory power than that of phenotypic procedures. This review focuses on the importance and the basis of molecular typing methods along with some considerations on de-sign and selection of probiotic culture for human consumption.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.1
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• pp.131-138
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• 2009

Among rumen microbes, bacteria play important roles in the biological degradation of plant fiber due to their large biomass and high activity. To maximize the utilization of fiber components such as cellulose and hemicellulose by ruminant animals, the ecology and functions of rumen bacteria should be understood in detail. Recent genome sequencing analyses of representative fibrolytic bacterial species revealed that the number and variety of enzymes for plant fiber digestion clearly differ between Fibrobacter succinogenes and Ruminococcus flavefaciens. Therefore, the mechanism of plant fiber digestion is also thought to differ between these two species. Ecology of individual fibrolytic bacterial species has been investigated using pure cultures and electron microscopy. Recent advances in molecular biology techniques complement the disadvantages of conventional techniques and allow accurate evaluation of the ecology of specific bacteria in mixed culture, even in situ and in vivo. Molecular monitoring of fibrolytic bacterial species in the rumen indicated the predominance of F. succinogenes. Nutritive interactions between fibrolytic and non-fibrolytic bacteria are important in maintaining and promoting fibrolytic activity, mainly in terms of crossfeeding of metabolites. Recent 16S rDNA-based analyses suggest that presently recognized fibrolytic species such as F. succinogenes and two Ruminococcus species with fibrolytic activity may represent only a small proportion of the total fibrolytic population and that uncultured bacteria may be responsible for fiber digestion in the rumen. Therefore, characterization of these unidentified bacteria is important to fully understand the physiology and ecology of fiber digestion. To achieve this, a combination of conventional and modern techniques could be useful.

• Fashion & Textile Research Journal
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• v.20 no.3
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• pp.331-342
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• 2018

This paper enables stakeholders involved in textile industry to gain an overview of standards used for care labelling and help establish a common standard that could be used as a universal standard. This study provides a comprehensive and detailed analytical study of care labelling standards adopted by common countries in the textile market. It was found that the development of a universal system for care labeling could enhance the trade of textile articles and assist consumers in caring for textile articles. Universal care label systems could be characterized by two main features of inclusiveness and comprehensiveness. The range of instructions and symbols presented were found different among standards. Insignificant differences in symbols' shapes were found between standards for bleaching, ironing and professional cleaning. The washing process had the widest variety of instructions; in addition, options were provided by stated standards. Different meanings were found for similar shapes in some tumble drying symbols. The study findings show the importance of enhancing text based standards or the development of an understandable format across as many cultures as possible. The unification of symbols and meanings may be needed to provide global consumers consistent guidance. The efficiency of a detailed standard that provides and covers a wide range of instructions is an important aspect. The visibility and practicality of offering variable options/symbols in one standard is an important aspect for developing a universal care label system.

• Mycobiology
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• v.51 no.3
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• pp.157-163
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• 2023

Cordyceps fumosorosea is an important species in the genus of Cordyceps, containing a variety of bioactive compounds, including fumosorinone (FU). This study was a ground-breaking assessment of FU levels in liquid and solid cultures. The present study focused on the impacts of solid-state fermentation (SSF) using solid substrates (wheat, oat, and rice), as well as the effects of fermentation parameters (pH, temperature, and incubation period), on the generation of FU. All the fermentation parameters had significant effects on the synthesis of FU. In a study of 25 ℃, 5.5 pH, and 21 days of incubation period combinations calculated -to give maximal FU production, it was found that the optimal values were 25 ℃, 5.5 pH, and 21 days, respectively. In a solid substrate medium culture, FU could be produced from SSF. At 30 days, a medium composed of rice yielded the most FU (798.50 mg/L), followed by a medium composed of wheat and oats (640.50 and 450.50 mg/L), respectively. An efficient method for increasing FU production on a large scale could be found in this approach. The results of this study might have multiple applications in different industrial fermentation processes.

• Fashion & Textile Research Journal
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• v.8 no.5
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• pp.523-529
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• 2006

The purpose of this study is to investigate the costumes of the ruling class in the Asuka-Nara Period by looking at the change in the clothing law of the era. During the Asuka-Nara period, various cultures such as Buddhism, architecture, sculpture, paintings, music, and so on were introduced through vigorous exchange with Chosun and Tang. Contrary to the primitive-Kohun Period, the regulation about costumes was enacted as law in the Asuka-Nara Period this fact tells us that there was high interest in clothing. Frequent reorganization of clothing law had to do with the rank system of Japan as well as with the exchange with other countries. The clothing law of Asuka-Nara Period was mostly consisted of the regulations about costumes of the ruling class including the royal family and government officials. The law regulated different coronet and color for the clothing depending on the different rank of the government officials. The more classified the rank was, the more varied color was used. In addition, there was a variety in the costumes system; 2 piece clothing of the Kohun Period was continuously used while new types of clothing were also introduced. The royal family members and government officials wore different types of clothing such as Yebok, Jobok, and Jebok, depending on time, place, or the purpose of occasion. The costumes of this period could be inferred from the analysis of the relics.

• Journal of Dairy Science and Biotechnology
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• v.21 no.2
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• pp.88-96
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• 2003

Cheese flavor is derived from three main pathways, that are proteolysis, lipolysis and glycolysis, the extent of which varies according to the cheese variety. Proteolysis is the most complex of the three primary events during cheese ripening. The basis of EMC technology is the use of specific enzymes acting at optimum conditions to produce required cheese flavors from suitable substrates. These enzymes consist of proteinases, peptidases, lipases, esterases. The key factors in EMC production are the type of cheese flavor required, the type and specificity of enzyme or cultures used, their concentration and some processing parameters, such as pH, temperature, agitation, aeration, and incubation time. The emulsifiers, bacteriocins, flavor compounds, and precursors also effect to it importantly. The dosage of enzyme or starter culture used is dependent on the intensity of flavor required, processing time and temperature and the quality of the initial substrate. To produce a consistent EMC product it is necessary to have a highly controlled process, and a detailed knowledge of the enzymatic reactions under the conditions used must be fully understood.